Flower shaped antimony oxide (Sb2O3) microstructures were synthesized in a large quantity via simple solution method using aqueous mixtures of antimony chloride and hexamethylene diamine (HMDA). The morphological char...Flower shaped antimony oxide (Sb2O3) microstructures were synthesized in a large quantity via simple solution method using aqueous mixtures of antimony chloride and hexamethylene diamine (HMDA). The morphological characterizations were done by field emission scanning electron microscopy (FESEM), which revealed that the synthesized products possess flower-shaped microstructures. The detailed structural characterizations performed by X-ray diffraction (XRD), Fourier transform infrared spectrophotometer (FT-IR) and Raman spectrophotometer confirmed that the synthesized microstructures are well-crystalline antimony oxide. The Energy dispersive spectroscopy (EDS) shows that the grown products are composed of Sb and O. Optical properties of the synthesized products were characterized by UV-Visible spectrophotometer which exhibits a well defined peak at ~ 291.0 nm. The photo-catalytic activity of the Sb2O3 microstructures was evaluated by degradation of acridine orange (AO), which mineralized almost 63.0% in 150 min. The chemical sensing properties of Sb2O3 microstructures was also studied by I-V technique using chloroform as a detecting solvent. The fabricated chloroform sensor demonstrates good sensitivity of 0.1154 μA cm–2 mM–1, lower-detection limit (~0.1 mM), large-linear dynamic range (LDR, 0.122 mM to 1.22 M) with linearity (R = 0.7898) in short response time (10.0 sec).展开更多
In this work, noncovalent functionalization of multiwalled carbon nanotube （MWNT） with acridine orange （AO） by electropolymerization is studied. The obtained composite film is a viable alternate electrode material...In this work, noncovalent functionalization of multiwalled carbon nanotube （MWNT） with acridine orange （AO） by electropolymerization is studied. The obtained composite film is a viable alternate electrode material, non-toxic, chemical inert, not volatile, using to construct modified electrode. The new type modified electrode has both of unique properties of MWNT and poly acridine orange （POAO）, can provide good sensitivity, low limits of detection, good response precision, and superb response stability.展开更多
We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a h...We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a high-performance liquid chromatography tandem diode array detector, electrospray ionization-ion-trap time-of-flight mass spectrometry and DNA- acridine orange fluorescence detection （HPLC-DAD-MSn-DNA-AO-FLD）. A large amount of information could be simultaneously obtained during one run, which included HPLC fingerprint, ultraviolet spectra, total ion chromatograms, MSn data of high-resolution mass spectrometry and activity profile of each compound binding with DNA. The method also provided information on structureactivity relationships and mechanism of interaction. We used this on-line method to identify five DNA-binding activity components from Lithospermum erythrorhizon sample for the first time. The result showed that the parent nucleus of shikonin derivatives could bind with DNA. The structure-activity relationship showed that the parent nucleus of shikonin derivatives plays a major role in DNA binding, not the carboxyl group on the side chain. This simple, rapid, high precision and good stability on-line method should be useful for compound separation, structural identification and screening of DNA-binding compounds in herbal medicines.展开更多
The surface enhanced fluorescence effect of acridine orange fluorophore in the proximity of Au nanoparticles has been investigated experimentally in the system of aqueous solution.Significant enhancement of the fluore...The surface enhanced fluorescence effect of acridine orange fluorophore in the proximity of Au nanoparticles has been investigated experimentally in the system of aqueous solution.Significant enhancement of the fluorescence intensity was observed when the system was excited with 532 nm or 442 nm CW lasers.The influence of the distances between neighboring Au particles as well as that between the fluorophore molecules and the Au surface were explored experimentally.The results demonstrated that a compact distribution of metallic particles was able to produce stronger fluorescence enhancement.Proper separation between the fluorophore molecules and the metal surface was favorable for a better enhancement.展开更多
A simple and sensitive method for detection of captopril was established based on its obstructive effect on nanomaterial sur- face energy transfer （NSET）. It was found that the acridine orange （AO） could be adsorb...A simple and sensitive method for detection of captopril was established based on its obstructive effect on nanomaterial sur- face energy transfer （NSET）. It was found that the acridine orange （AO） could be adsorbed onto the surface of citrated-gold nanoparticles （AuNPs） through electrostatic interaction. Incidentally, the fluorescence of AO was quenched owing to the dipole-dipole interaction of NSET between AO fluorophore and the AuNPs. However, captopril could obstruct the occurrence of NSET between AO and AuNPs effectively with the formation of Au-S covalent bonds between it and the AuNPs. Consequently, AO molecules were moved away from the surface of AuNPs leading to a decline of the energy transfer efficiency. Moreover, the fluorescence of AO could be gradually restored with the addition of captopril. Under the optimal conditions, the recovered fluorescence intensity correlated linearly with the concentration of captopril in the range of 400 nmol/L-2.0μmol/L with a detection limit of 71 μmol/L. Besides, the proposed method was successfully applied for the detection of captopril in troches with the recovery of 93%-102% and the RSD lower than 2.24%. The results were in good agreement with those obtained from the HPLC method,展开更多
文摘Flower shaped antimony oxide (Sb2O3) microstructures were synthesized in a large quantity via simple solution method using aqueous mixtures of antimony chloride and hexamethylene diamine (HMDA). The morphological characterizations were done by field emission scanning electron microscopy (FESEM), which revealed that the synthesized products possess flower-shaped microstructures. The detailed structural characterizations performed by X-ray diffraction (XRD), Fourier transform infrared spectrophotometer (FT-IR) and Raman spectrophotometer confirmed that the synthesized microstructures are well-crystalline antimony oxide. The Energy dispersive spectroscopy (EDS) shows that the grown products are composed of Sb and O. Optical properties of the synthesized products were characterized by UV-Visible spectrophotometer which exhibits a well defined peak at ~ 291.0 nm. The photo-catalytic activity of the Sb2O3 microstructures was evaluated by degradation of acridine orange (AO), which mineralized almost 63.0% in 150 min. The chemical sensing properties of Sb2O3 microstructures was also studied by I-V technique using chloroform as a detecting solvent. The fabricated chloroform sensor demonstrates good sensitivity of 0.1154 μA cm–2 mM–1, lower-detection limit (~0.1 mM), large-linear dynamic range (LDR, 0.122 mM to 1.22 M) with linearity (R = 0.7898) in short response time (10.0 sec).
文摘In this work, noncovalent functionalization of multiwalled carbon nanotube （MWNT） with acridine orange （AO） by electropolymerization is studied. The obtained composite film is a viable alternate electrode material, non-toxic, chemical inert, not volatile, using to construct modified electrode. The new type modified electrode has both of unique properties of MWNT and poly acridine orange （POAO）, can provide good sensitivity, low limits of detection, good response precision, and superb response stability.
基金Peking University Comprehensive Platform for Innovative Drug Research and Development(Grant No.2009ZX--09301--010)
文摘We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a high-performance liquid chromatography tandem diode array detector, electrospray ionization-ion-trap time-of-flight mass spectrometry and DNA- acridine orange fluorescence detection （HPLC-DAD-MSn-DNA-AO-FLD）. A large amount of information could be simultaneously obtained during one run, which included HPLC fingerprint, ultraviolet spectra, total ion chromatograms, MSn data of high-resolution mass spectrometry and activity profile of each compound binding with DNA. The method also provided information on structureactivity relationships and mechanism of interaction. We used this on-line method to identify five DNA-binding activity components from Lithospermum erythrorhizon sample for the first time. The result showed that the parent nucleus of shikonin derivatives could bind with DNA. The structure-activity relationship showed that the parent nucleus of shikonin derivatives plays a major role in DNA binding, not the carboxyl group on the side chain. This simple, rapid, high precision and good stability on-line method should be useful for compound separation, structural identification and screening of DNA-binding compounds in herbal medicines.
基金supported by the Foundation for the Key Project of the Chinese Ministry of Education (Grant No. 108118)the Fundamental Research Funds for the Central Universities (Grant No. GK200901022)
文摘The surface enhanced fluorescence effect of acridine orange fluorophore in the proximity of Au nanoparticles has been investigated experimentally in the system of aqueous solution.Significant enhancement of the fluorescence intensity was observed when the system was excited with 532 nm or 442 nm CW lasers.The influence of the distances between neighboring Au particles as well as that between the fluorophore molecules and the Au surface were explored experimentally.The results demonstrated that a compact distribution of metallic particles was able to produce stronger fluorescence enhancement.Proper separation between the fluorophore molecules and the metal surface was favorable for a better enhancement.
基金the National Natural Science Foundation of China(21175109)the special fund of Chongqing key laboratory(CSTC)for financial assistance
文摘A simple and sensitive method for detection of captopril was established based on its obstructive effect on nanomaterial sur- face energy transfer （NSET）. It was found that the acridine orange （AO） could be adsorbed onto the surface of citrated-gold nanoparticles （AuNPs） through electrostatic interaction. Incidentally, the fluorescence of AO was quenched owing to the dipole-dipole interaction of NSET between AO fluorophore and the AuNPs. However, captopril could obstruct the occurrence of NSET between AO and AuNPs effectively with the formation of Au-S covalent bonds between it and the AuNPs. Consequently, AO molecules were moved away from the surface of AuNPs leading to a decline of the energy transfer efficiency. Moreover, the fluorescence of AO could be gradually restored with the addition of captopril. Under the optimal conditions, the recovered fluorescence intensity correlated linearly with the concentration of captopril in the range of 400 nmol/L-2.0μmol/L with a detection limit of 71 μmol/L. Besides, the proposed method was successfully applied for the detection of captopril in troches with the recovery of 93%-102% and the RSD lower than 2.24%. The results were in good agreement with those obtained from the HPLC method,
