The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli...The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli at all those frequencies have a larger total number and a larger total length of roots and a smaller permeability of root plasma-membrane, compared with those cultivated in an environment without vibration stress. Vibration at respectively 1 Hz, 2 Hz, 3 Hz and 4 Hz enhances root activity and the 3 Hz vibration is the most favorable. There is an obvious negative correlation between root activity and permeability of root plasma-membrane. The effects may be explained by the likelihood that mechanical vibration at an appropriate frequency facilitates roots’ absorbing water and minerals which are indispensable to inducing and synthesizing in roots some active substances favorable to growth. Nevertheless, overstress damages the integrity of root plasm-membrane, increases the permeability, and results in the disability of protecting root cells.展开更多
This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-ins...This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-instigated dyslipidemia, hepatic steatosis, oxidative stress, insulin resistance, and fatty acid metabolism disorders in rats. In general, the above abnormalities were improved after 10 weeks intervention of ACF, ACP, and ACFP. Especially, ACFP considerably ameliorated HF-induced abnormal changes in body weight gain, serum TC, TG, LDL-C and HDL-C levels, as well as serum and hepatic SFAs, MUFAs and PUFAs contents. ACFP also alleviated HF-induced hyperglycemia and hyperinsulinemia, stabilized HF-caused increase in hepatic MDA and serum ALT, AST levels, and restored HF-declined hepatic T-SOD and GSH-Px activities. Besides, histopathology of the liver further endorsed the protective effects of ACFP on hepatocellular injury. Moreover, ACFP increased HF-dropped acetic, propionic and butyric acid levels. Overall, ACFP employs more efficacious protective effects against HF-induced metabolic disorders and liver damage than ACF and ACP. This study delivers a scientific foundation for developing kiwifruit(counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.展开更多
Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To inves...Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.展开更多
A new polysaccharide compound (ACPS-R) has recently been isolated from the root of Actinidia Chinensis Planch. When given inttaperitoneally to the transplantable tumor bearing mice at dose of 75-125 mg/kg, the tumor i...A new polysaccharide compound (ACPS-R) has recently been isolated from the root of Actinidia Chinensis Planch. When given inttaperitoneally to the transplantable tumor bearing mice at dose of 75-125 mg/kg, the tumor inhibition rate was more than 88.8% in Ehrilich ascitic cancer (EAC) or ascitic from of hepatoma (HepA) and more than 49.6% in solid hepatoma (HepS). The treatment effect of ACPS-R on EAC at dose of 80-100 mg kg and 125 mg/kg were comparable to that of cyclophosphamide at dose of 15 mg/kg and 22.5 mg/kg, respectively. ACPS-R could also prolong the life of EAC- or P388-bearing mice, and increase the percentage of EAC-free mice. In addition, when ACPS-R was used in combination with 5-Fu, the antitumor effect was enhanced as compared with 5-Fu alone. A marked increase in cAMP levels end cAMP cGMP ratio of spleen of EAC-bearing mice were observed after treatment of ACPS-R. The increase of both para meters nearly reached the normal levels of healthy mice. The increases of cAMP, cAMP cGMP and tumor remission had statistical significance. It showed an intermediate inhibitory effect of ACPS-R on DNA synthesis by incorporating 3H-TdR into EAC cells.The results indicated that ACPS-R acts as a new antitumor polysaccharide, and the treatment effect of Actinidia root in folk medicine is probably related to ACPS-R.展开更多
The effects of ethephon and abscisic acid(ABA) application on ripening-related genes of pre-harvest ‘Kohi' kiwifruit(Actinidia chinensis) were studied to clarify the possibility of the fruit ripening on the vine....The effects of ethephon and abscisic acid(ABA) application on ripening-related genes of pre-harvest ‘Kohi' kiwifruit(Actinidia chinensis) were studied to clarify the possibility of the fruit ripening on the vine. The fruits were treated on the vine at mature stage with 250 μL·L^(-1)ethephon or 100 μmol·L^(-1)ABA, and the expression levels of chlorophyll synthase(Ac CLS), chlorophyllase1(Ac CLH1), polygalacturonase(Ac PG), expansin(Ac EXP),β-amylase(Acβ-AM), sucrose synthase(Ac SUSY), MADS-box SEPALLATA4/RIPENING INHIBITOR-like(Ac SEP4/RIN) and FRUITFUL-like(Ac TDR4/FUL) genes were analyzed. The expression levels of Ac PG, Ac EXP, Acβ-AM, and Ac SUSY increased in the ethephon-treated fruit, but those of Ac CLH1 at 6and 9 days after treatment and Ac CLS decreased. Moreover, the expression levels of Ac SEP4/RIN and Ac TDR4/FUL, the latter of which is associated with ethylene biosynthesis, were higher in the ethephon-treated fruit. The expression level of each gene in ABA-treated fruit was not significantly different from that of the untreated control. The results suggest that ethephon application increases the expression levels of Ac PG,Ac EXP, Acβ-AM, Ac SUSY, Ac SEP4/RIN, and Ac TDR4/FUL in ‘Kohi' kiwifruit on the vine.展开更多
Objective Radix of Actinidiae chinensis,a medicinal plant used in China,is the dry root of Actini-diaceae plant and has been extensively employed to treat cancers of various organs,including the lung,liver and digesti...Objective Radix of Actinidiae chinensis,a medicinal plant used in China,is the dry root of Actini-diaceae plant and has been extensively employed to treat cancers of various organs,including the lung,liver and digestive system.However,up to now,its active antitumor and antiviral fractions remain unclear.The main purpose of this study is to identify the antitumor and antiviral sites of Radix of Actinidiae chinensis,which provides evidences for its further development.Methods Radix of Actinidiae chinensis was extracted by the refluxing sequentially with acetidin,ethanol and water,followed by the column chromatography and thin layer chromatography.The antitumor effects on Bel-7402,SW-620 and MDA-MB-231 tumor cells were investigated to screen the active antitumor sites by cell inhibition,the growth curve and the apoptosis staining.Meanwhile,to screen the active anti-hepatovirus fractions,the cell growth and the secretion of HBsAg and HBeAg in HepG2.2.15 cells were evaluated by the MTT test and enzyme-linked immunosorbent assay respectively.Results The acetidin fraction Y(Y1+Y2),the ethanol fraction A(A1+A3+A4) and B(A1+A4+A6),and the fraction compound C(A1+A4+Y1) have strong inhibitive effects on Bel-7402,SW-620 and MDA-MB-231 tumor cell lines.Moreover,the tumor cell apoptosis could be induced by the ethanol fraction B and the fraction compound C.In HepG2.2.15 cells,the treatment indexes of the acetidin fraction Y,the ethanol fraction A and the fraction compound C all exceeded 2 on the secretion of HBsAg,indicating the suppre-ssive effects of them on hepatitis B virus.The fraction yields of A,B and C exceeded 50%(60.28%,54.35% and 62.64% respectively),while that of Y did not(17.7%).Conclusion The ethanol fraction B and the fraction compound C are the antitumor sites of Actinidiae chinensis Radix,while the ethanol fraction A and the fraction compound C are its antiviral sites.展开更多
Tlie inhibitory actions of ethyl acetate,n-butanol and water fractions from Actinidia chinensis Radix (A. chinensis Radix) ethanol extracts on glucosidase were investigated. We found that the inhibitory activity of di...Tlie inhibitory actions of ethyl acetate,n-butanol and water fractions from Actinidia chinensis Radix (A. chinensis Radix) ethanol extracts on glucosidase were investigated. We found that the inhibitory activity of different ethanol extract fractions on α-glucosidase is concentration-dependent. At the same concentration, the ethyl acetate and n-butaiiol fractions showed higher inliibitory activities on gglucosidase in comparison witli acarbose. We then determined the chemical constituents of tlie ethyl acetate and n-butanol fractions through ultra performance liquid cliromatography-triple-time of flight/mass spectrometry(UPLC-Triple-TOF/MS). Based on the mass spectrometry analysis and the databases Scitinder and Reaxys, 21 compoiinds(A1-A21) were identified from the ethyl acetate fraction. In addition, 11 of those compounds(A2, A3, A5, A6, A8, A10, A11, A13, A15, A19, A20) were identified for the first time from A. chinensis Radix. We also identified 14 compounds(A22-A32 and A4-A6) from tlie w-butanol fraction and compound A29 is a new natural product. From the n-butanol fraction, 7 additional compounds (A22, A25, A27, A28, A30-A32) were identified from A. chinensis Radix for the first time.展开更多
The fruit development rules of two Chinensis Actinidia cultivars were studied in this paper. ‘Fengyue’has the same fruit development cycle as‘Cuiyu’. According to soluble solids content, there are four stages duri...The fruit development rules of two Chinensis Actinidia cultivars were studied in this paper. ‘Fengyue’has the same fruit development cycle as‘Cuiyu’. According to soluble solids content, there are four stages during fruit development phase named comparatively stable period, slow increase period, rapid increase period and stagnating increase period. Fruit firmness change exhibits two periods, i.e. slowly declining period and rapidly declining period. There is an obvious corresponding relation between the changes of fruit firmness and the soluble solids content, i.e. the rapidly declining time of fruit firmness corresponds with the rapidly increasing time of soluble solids content. According to the experiment, the best picking periods of ‘Fengyue’and ‘Cuiyu’are mid September to mid October and early October to early November, respectively.展开更多
基金Funded by the Natural Science Foundation of China (No. 39770206).
文摘The root development of Actinidia chinensis plantlets was studied in exposure to environmental stress of mechanical vibration at respectively 1 Hz, 2 Hz, 3 Hz, 4 Hz and 5 Hz. The plantlets exposed to vibration stimuli at all those frequencies have a larger total number and a larger total length of roots and a smaller permeability of root plasma-membrane, compared with those cultivated in an environment without vibration stress. Vibration at respectively 1 Hz, 2 Hz, 3 Hz and 4 Hz enhances root activity and the 3 Hz vibration is the most favorable. There is an obvious negative correlation between root activity and permeability of root plasma-membrane. The effects may be explained by the likelihood that mechanical vibration at an appropriate frequency facilitates roots’ absorbing water and minerals which are indispensable to inducing and synthesizing in roots some active substances favorable to growth. Nevertheless, overstress damages the integrity of root plasm-membrane, increases the permeability, and results in the disability of protecting root cells.
基金funded by the National Natural Science Foundation of China (C31901702,and 32072175)the China Postdoctoral Science Foundation (2019M653535,2020T130395)+2 种基金the Fundamental Research Funds for the Central Universities of Shaanxi Normal University in China (GK202003083)the grants of Sci-Tech Innovation Team of Shaanxi Province (2019TD-035)the Development Program for Innovative Research Team of Shaanxi Normal University (GK202101006)。
文摘This study aimed to investigate the protective effects of fleshes from two Actinidia chinensis(ACF), pericarps from two A. chinensis(ACP), and fleshes with pericarps from two A. chinensis(ACFP)on high fructose(HF)-instigated dyslipidemia, hepatic steatosis, oxidative stress, insulin resistance, and fatty acid metabolism disorders in rats. In general, the above abnormalities were improved after 10 weeks intervention of ACF, ACP, and ACFP. Especially, ACFP considerably ameliorated HF-induced abnormal changes in body weight gain, serum TC, TG, LDL-C and HDL-C levels, as well as serum and hepatic SFAs, MUFAs and PUFAs contents. ACFP also alleviated HF-induced hyperglycemia and hyperinsulinemia, stabilized HF-caused increase in hepatic MDA and serum ALT, AST levels, and restored HF-declined hepatic T-SOD and GSH-Px activities. Besides, histopathology of the liver further endorsed the protective effects of ACFP on hepatocellular injury. Moreover, ACFP increased HF-dropped acetic, propionic and butyric acid levels. Overall, ACFP employs more efficacious protective effects against HF-induced metabolic disorders and liver damage than ACF and ACP. This study delivers a scientific foundation for developing kiwifruit(counting peel)-based dietary supplements for those with glucolipid-metabolic disorders and liver damage.
基金funded by the National Natural Science Foundation of China(Project No.81973615,No.81803910).
文摘Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.
文摘A new polysaccharide compound (ACPS-R) has recently been isolated from the root of Actinidia Chinensis Planch. When given inttaperitoneally to the transplantable tumor bearing mice at dose of 75-125 mg/kg, the tumor inhibition rate was more than 88.8% in Ehrilich ascitic cancer (EAC) or ascitic from of hepatoma (HepA) and more than 49.6% in solid hepatoma (HepS). The treatment effect of ACPS-R on EAC at dose of 80-100 mg kg and 125 mg/kg were comparable to that of cyclophosphamide at dose of 15 mg/kg and 22.5 mg/kg, respectively. ACPS-R could also prolong the life of EAC- or P388-bearing mice, and increase the percentage of EAC-free mice. In addition, when ACPS-R was used in combination with 5-Fu, the antitumor effect was enhanced as compared with 5-Fu alone. A marked increase in cAMP levels end cAMP cGMP ratio of spleen of EAC-bearing mice were observed after treatment of ACPS-R. The increase of both para meters nearly reached the normal levels of healthy mice. The increases of cAMP, cAMP cGMP and tumor remission had statistical significance. It showed an intermediate inhibitory effect of ACPS-R on DNA synthesis by incorporating 3H-TdR into EAC cells.The results indicated that ACPS-R acts as a new antitumor polysaccharide, and the treatment effect of Actinidia root in folk medicine is probably related to ACPS-R.
文摘The effects of ethephon and abscisic acid(ABA) application on ripening-related genes of pre-harvest ‘Kohi' kiwifruit(Actinidia chinensis) were studied to clarify the possibility of the fruit ripening on the vine. The fruits were treated on the vine at mature stage with 250 μL·L^(-1)ethephon or 100 μmol·L^(-1)ABA, and the expression levels of chlorophyll synthase(Ac CLS), chlorophyllase1(Ac CLH1), polygalacturonase(Ac PG), expansin(Ac EXP),β-amylase(Acβ-AM), sucrose synthase(Ac SUSY), MADS-box SEPALLATA4/RIPENING INHIBITOR-like(Ac SEP4/RIN) and FRUITFUL-like(Ac TDR4/FUL) genes were analyzed. The expression levels of Ac PG, Ac EXP, Acβ-AM, and Ac SUSY increased in the ethephon-treated fruit, but those of Ac CLH1 at 6and 9 days after treatment and Ac CLS decreased. Moreover, the expression levels of Ac SEP4/RIN and Ac TDR4/FUL, the latter of which is associated with ethylene biosynthesis, were higher in the ethephon-treated fruit. The expression level of each gene in ABA-treated fruit was not significantly different from that of the untreated control. The results suggest that ethephon application increases the expression levels of Ac PG,Ac EXP, Acβ-AM, Ac SUSY, Ac SEP4/RIN, and Ac TDR4/FUL in ‘Kohi' kiwifruit on the vine.
基金Important National Science & Technology Specific Project (2009Z10603)Hunan Science andTechnology Project (2009FJ3209)
文摘Objective Radix of Actinidiae chinensis,a medicinal plant used in China,is the dry root of Actini-diaceae plant and has been extensively employed to treat cancers of various organs,including the lung,liver and digestive system.However,up to now,its active antitumor and antiviral fractions remain unclear.The main purpose of this study is to identify the antitumor and antiviral sites of Radix of Actinidiae chinensis,which provides evidences for its further development.Methods Radix of Actinidiae chinensis was extracted by the refluxing sequentially with acetidin,ethanol and water,followed by the column chromatography and thin layer chromatography.The antitumor effects on Bel-7402,SW-620 and MDA-MB-231 tumor cells were investigated to screen the active antitumor sites by cell inhibition,the growth curve and the apoptosis staining.Meanwhile,to screen the active anti-hepatovirus fractions,the cell growth and the secretion of HBsAg and HBeAg in HepG2.2.15 cells were evaluated by the MTT test and enzyme-linked immunosorbent assay respectively.Results The acetidin fraction Y(Y1+Y2),the ethanol fraction A(A1+A3+A4) and B(A1+A4+A6),and the fraction compound C(A1+A4+Y1) have strong inhibitive effects on Bel-7402,SW-620 and MDA-MB-231 tumor cell lines.Moreover,the tumor cell apoptosis could be induced by the ethanol fraction B and the fraction compound C.In HepG2.2.15 cells,the treatment indexes of the acetidin fraction Y,the ethanol fraction A and the fraction compound C all exceeded 2 on the secretion of HBsAg,indicating the suppre-ssive effects of them on hepatitis B virus.The fraction yields of A,B and C exceeded 50%(60.28%,54.35% and 62.64% respectively),while that of Y did not(17.7%).Conclusion The ethanol fraction B and the fraction compound C are the antitumor sites of Actinidiae chinensis Radix,while the ethanol fraction A and the fraction compound C are its antiviral sites.
文摘Tlie inhibitory actions of ethyl acetate,n-butanol and water fractions from Actinidia chinensis Radix (A. chinensis Radix) ethanol extracts on glucosidase were investigated. We found that the inhibitory activity of different ethanol extract fractions on α-glucosidase is concentration-dependent. At the same concentration, the ethyl acetate and n-butaiiol fractions showed higher inliibitory activities on gglucosidase in comparison witli acarbose. We then determined the chemical constituents of tlie ethyl acetate and n-butanol fractions through ultra performance liquid cliromatography-triple-time of flight/mass spectrometry(UPLC-Triple-TOF/MS). Based on the mass spectrometry analysis and the databases Scitinder and Reaxys, 21 compoiinds(A1-A21) were identified from the ethyl acetate fraction. In addition, 11 of those compounds(A2, A3, A5, A6, A8, A10, A11, A13, A15, A19, A20) were identified for the first time from A. chinensis Radix. We also identified 14 compounds(A22-A32 and A4-A6) from tlie w-butanol fraction and compound A29 is a new natural product. From the n-butanol fraction, 7 additional compounds (A22, A25, A27, A28, A30-A32) were identified from A. chinensis Radix for the first time.
文摘The fruit development rules of two Chinensis Actinidia cultivars were studied in this paper. ‘Fengyue’has the same fruit development cycle as‘Cuiyu’. According to soluble solids content, there are four stages during fruit development phase named comparatively stable period, slow increase period, rapid increase period and stagnating increase period. Fruit firmness change exhibits two periods, i.e. slowly declining period and rapidly declining period. There is an obvious corresponding relation between the changes of fruit firmness and the soluble solids content, i.e. the rapidly declining time of fruit firmness corresponds with the rapidly increasing time of soluble solids content. According to the experiment, the best picking periods of ‘Fengyue’and ‘Cuiyu’are mid September to mid October and early October to early November, respectively.
