Synthesis of functional nanostructures with the least number of tests is paramount towards the propelling materials development. However, the synthesis method containing multivariable leads to high uncertainty, exhaus...Synthesis of functional nanostructures with the least number of tests is paramount towards the propelling materials development. However, the synthesis method containing multivariable leads to high uncertainty, exhaustive attempts, and exorbitant manpower costs. Machine learning (ML) burgeons and provokes an interest in rationally designing and synthesizing materials. Here, we collect the dataset of nano-functional materials carbon dots (CDs) on synthetic parameters and optical properties. ML is applied to assist the synthesis process to enhance photoluminescence quantum yield (QY) by building the methodology named active adaptive method (AAM), including the model selection, max points screen, and experimental verification. An interactive iteration strategy is the first time considered in AAM with the constant acquisition of the furnished data by itself to perfect the model. CDs exhibit a strong red emission with QY up to 23.3% and enhancement of around 200% compared with the pristine value obtained through the AAM guidance. Furthermore, the guided CDs are applied as metal ions probes for Co^(2+) and Fe^(3+), with a concentration range of 0–120 and 0–150 µM, and their detection limits are 1.17 and 0.06 µM. Moreover, we also apply CDs for dental diagnosis and treatment using excellent optical ability. It can effectively detect early caries and treat mineralization combined with gel. The study shows that the error of experiment verification gradually decreases and QY improves double with the effective feedback loops by AAM, suggesting the great potential of utilizing ML to guide the synthesis of novel materials. Finally, the code is open-source and provided to be referenced for further investigation on the novel inorganic material prediction.展开更多
Natural enzymes,owing to their outstanding catalytic efficiency and substrate specificity,have been used in a variety of applications including clinical diagnosis,environmental monitoring and wastewater treatment.Howe...Natural enzymes,owing to their outstanding catalytic efficiency and substrate specificity,have been used in a variety of applications including clinical diagnosis,environmental monitoring and wastewater treatment.However,they face inevitable problems such as relatively high cost and lack of stability,dramatically hindering their practical applications in the industry.Recently,a class of nanomaterial that possesses intrinsic enzyme-like properties,nanozyme,has emerged exhibiting numerous advantages over its natural counterpart and has been used as a viable enzyme alternative.In the past decade there are many reviews on nanozyme.The previous discussions tend to view nanozyme as a type of nanomaterial rather than an enzyme.However,it is the enzyme-like activity of nanozymes that provides foundation for their application and nanozymes with the same enzymatic activity usually have some regularity in application.Herein,in this review,we attempt to classify nanozymes by their enzyme-like activity to explain the application principle and relevant cases of nanozymes in clinical diagnosis,environmental monitoring and wastewater treatment,expecting to promote deeper thinking of nanozymes as enzyme mimics and provide useful guidance for future research.展开更多
Tumor vascular normalization has emerged as a promising strategy for synergistic therapy recently.Based on the strategy of“fluorescence turn on-controllable release”,a novel bifunctional candidate was con-structed b...Tumor vascular normalization has emerged as a promising strategy for synergistic therapy recently.Based on the strategy of“fluorescence turn on-controllable release”,a novel bifunctional candidate was con-structed based on previous developed vascular normalization inducer QDAU5,which could self-assemble to form functional enzyme infrared QDAU5 nanoparticles(FEIRQ NPs).Subsequently,biological evaluation demonstrated that the FEIRQ NPs could induce ferroptosis,endoplasmic reticulum stress,and antigen pre-conditioning and maturation of dendritic cells and CD8^(+)T cells,leading to excellent antitumor efficacy in the absence of cytotoxic drugs.Additionally,FEIRQ NPs show high fluorescence intensity upon expo-sure to theβ-galactosidase(β-Gal)enzyme expressed in ovarian cancer,enabling real-time monitoring of therapeutic effects.Overall,our findings suggest a prospering strategy to early diagnosis and efficient therapy for ovarian cancer without cytotoxicity.展开更多
目的探究在神经病学临床实习教学中实施多学科诊疗(Multidisciplinary Diagnosis and Treatment,MDT)模式下问题为导向(Problem-based Learning,PBL)教学法的效果。方法选取2023年1—12月东台市人民医院神经病学科的60名规培生为研究对...目的探究在神经病学临床实习教学中实施多学科诊疗(Multidisciplinary Diagnosis and Treatment,MDT)模式下问题为导向(Problem-based Learning,PBL)教学法的效果。方法选取2023年1—12月东台市人民医院神经病学科的60名规培生为研究对象,以教学方法的不同分为观察组、参照组,各30名。参照组行PBL教学法,观察组行MDT模式下PBL教学法。比较两组带教前后的考核成绩(理论知识和实践能力)、临床工作能力(自学能力、临床分析能力、临床思维能力及团队合作能力)及带教满意程度。结果观察组的考核成绩、临床工作能力及带教满意程度均高于参照组,差异有统计学意义(P均<0.05)。结论MDT模式下PBL教学法能够改善神经病学临床实习教学中规培生考核成绩,改善临床工作能力,提高带教满意程度。展开更多
目的探究重症监护室(Intensive Care Unit,ICU)脑出血术后应用亚低温治疗的临床效果。方法方便选择2020年1月—2023年12月福建医科大学附属三明市第一医院脑出血术后患者152例为研究对象,依据奇偶数法分为两组,各76例。对照组术后予以...目的探究重症监护室(Intensive Care Unit,ICU)脑出血术后应用亚低温治疗的临床效果。方法方便选择2020年1月—2023年12月福建医科大学附属三明市第一医院脑出血术后患者152例为研究对象,依据奇偶数法分为两组,各76例。对照组术后予以常规治疗、研究组术后予以常规治疗+亚低温治疗。比较两组治疗效果、并发症、神经功能、炎症因子、血清指标、活动能力。结果研究组治疗有效率为97.37%,高于对照组的86.84%,差异有统计学意义(χ^(2)=5.790,P=0.016)。研究组并发症总发生率为3.95%,对照组为2.63%,两组比较,差异无统计学意义(P>0.05)。治疗后,研究组神经功能优于对照组,差异有统计学意义(P<0.05)。治疗后,研究组C反应蛋白、白细胞介素-6水平均低于对照组,差异有统计学意义(P均<0.05)。治疗后,研究组血管升压素及内皮素均低于对照组,差异有统计学意义(P均<0.05)。治疗后,研究组上肢、下肢活动能力均优于对照组,差异有统计学意义(P均<0.05)。结论亚低温治疗在ICU脑出血术后治疗中应用效果显著。展开更多
慢性活动性EB病毒感染(Chronic active EBV infection,CAEBV)是一种少见的发生在无明确免疫缺陷个体的综合征,临床表现多种多样,其病理改变几乎可涉及到各个器官。主要表现为EBV感染后出现慢性或复发性传单样症状,伴随EBV抗体的异常改...慢性活动性EB病毒感染(Chronic active EBV infection,CAEBV)是一种少见的发生在无明确免疫缺陷个体的综合征,临床表现多种多样,其病理改变几乎可涉及到各个器官。主要表现为EBV感染后出现慢性或复发性传单样症状,伴随EBV抗体的异常改变或病毒载量的升高,病程中可出现严重的或致死的并发症。文章就其发病机制、临床表现、实验室检查及诊治方案进展作一介绍,以提高临床对慢性活动性EBV的诊治水平。展开更多
基金the support from Beijing National Science Foundation(No.L222109)the Military Health Care Project(No.22BJZ22)+1 种基金Q.X.acknowledges the support from the National Natural Science Foundation of China(No.52211530034)the Beijing National Science Foundation(No.3222018).
文摘Synthesis of functional nanostructures with the least number of tests is paramount towards the propelling materials development. However, the synthesis method containing multivariable leads to high uncertainty, exhaustive attempts, and exorbitant manpower costs. Machine learning (ML) burgeons and provokes an interest in rationally designing and synthesizing materials. Here, we collect the dataset of nano-functional materials carbon dots (CDs) on synthetic parameters and optical properties. ML is applied to assist the synthesis process to enhance photoluminescence quantum yield (QY) by building the methodology named active adaptive method (AAM), including the model selection, max points screen, and experimental verification. An interactive iteration strategy is the first time considered in AAM with the constant acquisition of the furnished data by itself to perfect the model. CDs exhibit a strong red emission with QY up to 23.3% and enhancement of around 200% compared with the pristine value obtained through the AAM guidance. Furthermore, the guided CDs are applied as metal ions probes for Co^(2+) and Fe^(3+), with a concentration range of 0–120 and 0–150 µM, and their detection limits are 1.17 and 0.06 µM. Moreover, we also apply CDs for dental diagnosis and treatment using excellent optical ability. It can effectively detect early caries and treat mineralization combined with gel. The study shows that the error of experiment verification gradually decreases and QY improves double with the effective feedback loops by AAM, suggesting the great potential of utilizing ML to guide the synthesis of novel materials. Finally, the code is open-source and provided to be referenced for further investigation on the novel inorganic material prediction.
基金supported by the National Natural Science Foundation of China(grant No.81930050,No.82122037,No.31900981)the Strategic Priority Research Program of Chinese Academy of Sciences(CAS)(grant No.XDB29040101)+4 种基金Chinese Academy of Sciences(grant No.YJKYYQ20180048)the Key Research Program of Frontier Sciences,CAS(grant No.QYZDYSSW-SMC013)National Key Research and Development Program of China(grant No.2017YFA0205501)Youth Innovation Promotion Association of Chinese Academy of Sciences(grant No.2019093)CAS Interdisciplinary Innovation Team(grant No.JCTD-2020-08).
文摘Natural enzymes,owing to their outstanding catalytic efficiency and substrate specificity,have been used in a variety of applications including clinical diagnosis,environmental monitoring and wastewater treatment.However,they face inevitable problems such as relatively high cost and lack of stability,dramatically hindering their practical applications in the industry.Recently,a class of nanomaterial that possesses intrinsic enzyme-like properties,nanozyme,has emerged exhibiting numerous advantages over its natural counterpart and has been used as a viable enzyme alternative.In the past decade there are many reviews on nanozyme.The previous discussions tend to view nanozyme as a type of nanomaterial rather than an enzyme.However,it is the enzyme-like activity of nanozymes that provides foundation for their application and nanozymes with the same enzymatic activity usually have some regularity in application.Herein,in this review,we attempt to classify nanozymes by their enzyme-like activity to explain the application principle and relevant cases of nanozymes in clinical diagnosis,environmental monitoring and wastewater treatment,expecting to promote deeper thinking of nanozymes as enzyme mimics and provide useful guidance for future research.
基金supported by the National Natural Science Foundation of China(NSFC,Nos.82373793,82173742)the Science Fund for Distinguished Young Scholars of Shaanxi Province(No.2022JC-54)the Key Research and Development Program of Shaanxi Province(No.2023-YBSF-131).
文摘Tumor vascular normalization has emerged as a promising strategy for synergistic therapy recently.Based on the strategy of“fluorescence turn on-controllable release”,a novel bifunctional candidate was con-structed based on previous developed vascular normalization inducer QDAU5,which could self-assemble to form functional enzyme infrared QDAU5 nanoparticles(FEIRQ NPs).Subsequently,biological evaluation demonstrated that the FEIRQ NPs could induce ferroptosis,endoplasmic reticulum stress,and antigen pre-conditioning and maturation of dendritic cells and CD8^(+)T cells,leading to excellent antitumor efficacy in the absence of cytotoxic drugs.Additionally,FEIRQ NPs show high fluorescence intensity upon expo-sure to theβ-galactosidase(β-Gal)enzyme expressed in ovarian cancer,enabling real-time monitoring of therapeutic effects.Overall,our findings suggest a prospering strategy to early diagnosis and efficient therapy for ovarian cancer without cytotoxicity.
文摘目的探究在神经病学临床实习教学中实施多学科诊疗(Multidisciplinary Diagnosis and Treatment,MDT)模式下问题为导向(Problem-based Learning,PBL)教学法的效果。方法选取2023年1—12月东台市人民医院神经病学科的60名规培生为研究对象,以教学方法的不同分为观察组、参照组,各30名。参照组行PBL教学法,观察组行MDT模式下PBL教学法。比较两组带教前后的考核成绩(理论知识和实践能力)、临床工作能力(自学能力、临床分析能力、临床思维能力及团队合作能力)及带教满意程度。结果观察组的考核成绩、临床工作能力及带教满意程度均高于参照组,差异有统计学意义(P均<0.05)。结论MDT模式下PBL教学法能够改善神经病学临床实习教学中规培生考核成绩,改善临床工作能力,提高带教满意程度。
文摘目的探究重症监护室(Intensive Care Unit,ICU)脑出血术后应用亚低温治疗的临床效果。方法方便选择2020年1月—2023年12月福建医科大学附属三明市第一医院脑出血术后患者152例为研究对象,依据奇偶数法分为两组,各76例。对照组术后予以常规治疗、研究组术后予以常规治疗+亚低温治疗。比较两组治疗效果、并发症、神经功能、炎症因子、血清指标、活动能力。结果研究组治疗有效率为97.37%,高于对照组的86.84%,差异有统计学意义(χ^(2)=5.790,P=0.016)。研究组并发症总发生率为3.95%,对照组为2.63%,两组比较,差异无统计学意义(P>0.05)。治疗后,研究组神经功能优于对照组,差异有统计学意义(P<0.05)。治疗后,研究组C反应蛋白、白细胞介素-6水平均低于对照组,差异有统计学意义(P均<0.05)。治疗后,研究组血管升压素及内皮素均低于对照组,差异有统计学意义(P均<0.05)。治疗后,研究组上肢、下肢活动能力均优于对照组,差异有统计学意义(P均<0.05)。结论亚低温治疗在ICU脑出血术后治疗中应用效果显著。
文摘慢性活动性EB病毒感染(Chronic active EBV infection,CAEBV)是一种少见的发生在无明确免疫缺陷个体的综合征,临床表现多种多样,其病理改变几乎可涉及到各个器官。主要表现为EBV感染后出现慢性或复发性传单样症状,伴随EBV抗体的异常改变或病毒载量的升高,病程中可出现严重的或致死的并发症。文章就其发病机制、临床表现、实验室检查及诊治方案进展作一介绍,以提高临床对慢性活动性EBV的诊治水平。
