Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects....Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects.展开更多
背景:磷酸钙骨水泥具有良好的自固定性、易成型性,良好的生物相容性和骨传导性,是一种出色的骨移植材料,但其仍然有机械强度差、降解速率慢、生物相容性比较低等问题。目的:探讨磷酸钙骨水泥改性相关的研究与临床应用。方法:通过计算机...背景:磷酸钙骨水泥具有良好的自固定性、易成型性,良好的生物相容性和骨传导性,是一种出色的骨移植材料,但其仍然有机械强度差、降解速率慢、生物相容性比较低等问题。目的:探讨磷酸钙骨水泥改性相关的研究与临床应用。方法:通过计算机检索Web of Science、PubMed、万方、中国知网,以“磷酸钙骨水泥、改性;Calcium phosphate bone cement、modification”为中、英文关键词,检索2014至2020年的文献。排除无关及重复文献,最终纳入72篇文献进行综述。结果与结论:目前为止,磷酸钙骨水泥与生物活性物质、金属与非金属元素、有机物、干细胞以不同的方式混合可提高其机械强度、生物相容性、促进骨再生等,虽然通过在骨水泥添加不同物质可以改善其部分性状,但目前还处于实验阶段,制备的磷酸钙骨水泥还不理想。在临床应用过程中,磷酸钙骨水泥应用于骨折、骨髓炎及骨肿瘤的缺损修补相较于传统治疗方式具有较好的疗效,然而在应用过程中发生了新生骨生长速率及骨水泥降解速率缓慢、注射部位严重感染、填充物塌陷等并发症。展开更多
基金the National Key R&D Program of China(Grant No.2018YFC1106300 and 2017YFC1105000)the National Natural Science Foundation of China(Grant No.52072398,51802340,31870956,81860385,81672227,U2001221,51772210)+2 种基金the Frontier Science Key Research Programs of CAS(Grant No.QYZDB-SSW-JSC030)the Shenzhen Significant Strategy Layout Project(Grant No.JCYJ20170413162104773 and JCYJ20200109114620793)Beijing Municipal Health Commission(Grant No.BMHC-2018-4,BMHC-2019-9,PXM2020_026275_000002).
文摘Calcium phosphate cements(CPC)are widely anticipated to be an optimum bone repair substitute due to its satisfied biocompatibility and degradability,suitable to be used in minimally invasive treatment of bone defects.However the clinical application of CPC is still not satisfied by its poor cohesiveness and mechanical properties,in particular its osteoinductivity.Hyaluronic acid reinforced calcium phosphate cements(HA/CPC)showed extroadinary potential not only enhancing the compressive strength of the cements but also significantly increasing its osteoinductivity.In our study,the compressive strength of HA/CPC increased significantly when the cement was added 1%hyaluronic acid(denoted as 1-HA/CPC).In the meantime,hyaluronic acid obviously promoted ALP activity,osteogenic related protein and mRNA expression of hBMSCs(human bone marrow mesenchymal stem cells)in vitro,cement group of HA/CPC with 4%hyaluronic acid adding(denoted as 4-HA/CPC)showed optimal enhancement in hBMSCs differentiation.After being implanted in rat tibial defects,4-HA/CPC group exhibited better bone repair ability and bone growth promoting factors,comparing to pure CPC and 1-HA/CPC groups.The underlying biological mechanism of this stimulation for HA/CPC may be on account of higher osteogenic promoting factors secretion and osteogenic genes expression with hyaluronic acid incorporation.These results indicate that hyaluronic acid is a highly anticipated additive to improve physicochemical properties and osteoinductivity performance of CPCs for minimally invasive healing of bone defects.
文摘背景:磷酸钙骨水泥具有良好的自固定性、易成型性,良好的生物相容性和骨传导性,是一种出色的骨移植材料,但其仍然有机械强度差、降解速率慢、生物相容性比较低等问题。目的:探讨磷酸钙骨水泥改性相关的研究与临床应用。方法:通过计算机检索Web of Science、PubMed、万方、中国知网,以“磷酸钙骨水泥、改性;Calcium phosphate bone cement、modification”为中、英文关键词,检索2014至2020年的文献。排除无关及重复文献,最终纳入72篇文献进行综述。结果与结论:目前为止,磷酸钙骨水泥与生物活性物质、金属与非金属元素、有机物、干细胞以不同的方式混合可提高其机械强度、生物相容性、促进骨再生等,虽然通过在骨水泥添加不同物质可以改善其部分性状,但目前还处于实验阶段,制备的磷酸钙骨水泥还不理想。在临床应用过程中,磷酸钙骨水泥应用于骨折、骨髓炎及骨肿瘤的缺损修补相较于传统治疗方式具有较好的疗效,然而在应用过程中发生了新生骨生长速率及骨水泥降解速率缓慢、注射部位严重感染、填充物塌陷等并发症。