Objective:To investigate the mechanism of JAK2/STAT3/SOCS1 signaling pathway in electroacupuncture of different acupoints on acute colitis rats.Methods:36 SPF SD rats were randomly divided into 6 groups,with 6 rats in...Objective:To investigate the mechanism of JAK2/STAT3/SOCS1 signaling pathway in electroacupuncture of different acupoints on acute colitis rats.Methods:36 SPF SD rats were randomly divided into 6 groups,with 6 rats in each group.The rat model of acute colitis was prepared by enema with glacial acetic acid solution.After the model was established,electroacupuncture was given to each acupoint group,with density wave,frequency 2Hz-50 Hz,intensity 2 mA,muscle tremor as the degree 20 min/time,1 time/day,for 3 consecutive days.Observe the general condition of rats;the pathological changes of colonic mucosa in rats were observed by HE method.The contents of serum interleukin-4(IL-4)and interleukin-8(IL-8)were detected by ELISA.Western blot and RT-PCR were used to detect the expression of JAK2,STAT3,SOCS1 protein and mRNA in rat colon tissue.Results:In contrast to the normal group,the overall condition of the model group was worse,the colonic mucosa was severely damaged,even necrotic,and the ulcer surface was obvious.The content of IL-4 in serum was obviously reduced,and the content of IL-8 was obviously go up(P<0.01).The protein content of JAK2,STAT3 and the expression of JAK2,STAT3 mRNA in colon tissue of rats were obviously go up,while the protein content of SOCS1 and the expression of SOCS1 mRNA were obviously reduced(P<0.01).In contrast to the model group,the general condition of rats in each acupoint group was significantly improved,the damage and necrosis of colonic mucosa and ulcer surface were obviously alleviated,the content of IL-4 in serum was obviously go up,and the content of IL-8 was significantly decreased(P<0.01).The protein content of JAK2,STAT3 and the expression of JAK2,STAT3 mRNA in colon tissue of rats were obviously reduced,while the protein content of SOCS1 and the expression of SOCS1 mRNA were obviously go up(P<0.05,P<0.01).Comparison of different acupoint groups,the colonic mucosal injury in the Zusanli group was significantly reduced,the content of serum IL-4 was significantly increased,and the content of IL-8 was significantly decreased(P<0.05,P<0.01).The protein content and mRNA expression of JAK2 and STAT3 in colon tissue were significantly down-regulated,while the protein content and mRNA expression of SOCS1 were significantly go up(P<0.05,P<0.01).Conclusion:Electroacupuncture at each acupoint can improve the damage of colonic mucosa and reduce the inflammatory response.The therapeutic effect of Zusanli(ST36)is better than that of Tianshu(ST25),Dachangshu(BL25)and Shangjuxu(ST37).The mechanism may be related to the regulation of JAK2/STAT3/SOCS1 signaling pathway related proteins and inflammatory cytokines IL-4 and IL-8.展开更多
Peripheral nerve injury not only affects the site of the injury, but can also induce neuronal apop- tosis at the spinal cord. However, many acupuncture clinicians still focus only on the injury site, selecting acupoin...Peripheral nerve injury not only affects the site of the injury, but can also induce neuronal apop- tosis at the spinal cord. However, many acupuncture clinicians still focus only on the injury site, selecting acupoints entirely along the injured nerve trunk and neglecting other regions; this may delay onset of treatment efficacy and rehabilitation. Therefore, in the present study, we compared the clinical efficacy of acupuncture at Governor vessel and local meridian acupoints combined (GV/LM group) with acupuncture at local meridian acupoints alone (LM group) in the treatment of patients with peripheral nerve injury. In the GV/LM group (n = 15), in addition to meridian acupoints at the injury site, the following acupoints on the Governor vessel were stimulated: Baihui (GV20), Fengfu (GV16), Dazhui (GV14), and Shenzhu (GV12), selected to treat nerve injury of the upper limb, and Jizhong (GV6), Mingmen (GV4), Yaoyangguan (GV3), and Yaoshu (GV2) to treat nerve injury of the lower limb. In the LM group (n = 15), only me- ridian acupoints along the injured nerve were selected. Both groups had electroacupuncture treatment for 30 minutes, once a day, 5 times per week, for 6 weeks. Two cases dropped out of the LM group. A good or excellent clinical response was obtained in 80% of the patients in the GV/ LM group and 38.5% of the LM group. In a second study, an additional 20 patients underwent acupuncture with the same prescription as the GV/LM group. Electomyographic nerve conduc- tion tests were performed before and after acupuncture to explore the mechanism of action of the treatment. An effective response was observed in 80.0% of the patients, with greater motor nerve conduction velocity and amplitude after treatment, indicating that electroacupuncture on specific Governor vessel acupoints promotes functional motor nerve repair after peripheral nerve injury. In addition, electromyography was performed before, during and after electroacu- puncture in one patient with radial nerve injury. After a single session, the patient's motor nerve conduction velocity increased by 23.2%, indicating that electroacupuncture at Governor vessel acupoints has an immediate therapeutic effect on peripheral nerve injury. Our results indicate that Governor vessel and local meridian acupoints used simultaneously promote functional repair after peripheral nerve injury. The mechanism of action may arise from an improvement of the local microenvironment in injured nervous tissue, as well as immediate effects of Governor vessel and local meridian acupoint stimulation to ensure the continuity between the peripheral and central nervous systems.展开更多
Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model...Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui(GV14),Jizhong(GV6),Houhui,Guanyuan(CV4)and Zhongwan(CV12).The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter(GLAST)and glial glutamate transporter-1(GLT-1)expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured(left)side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.展开更多
BACKGROUND: Based on clinical findings and syndromes, it has been previously shown that therapeutic methods for cerebral palsy can be greatly improved by selecting fewer, but more accurate acupoints. Therefore, great...BACKGROUND: Based on clinical findings and syndromes, it has been previously shown that therapeutic methods for cerebral palsy can be greatly improved by selecting fewer, but more accurate acupoints. Therefore, greater therapeutic efficacy for cerebral palsy could be obtained by selecting the five Shu acupoints, which are located at the qi-intersecting point of the twelve meridians below the elbow and knee. OBJECTIVE: To treat children with spastic cerebral palsy using acupuncture and massage at the Shu and He points through a combination of rehabilitative training, and to compare the effects with routine acupuncture and massage. DESIGN: A randomized controlled clinical trial. SETTING: Rehabilitation Center for Cerebral Palsy, Department of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine. PARTICIPANTS: Sixty children with spastic cerebral palsy were selected from the Rehabilitation Center for Cerebral Palsy, Department of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from May 2003 to February 2006. There were 38 boys and 22 girls, aged 10 months to 4 years old, muscle strength ranging from grade I to grade IV. The children were randomly divided into a treatment group (n =30) and a control group (n =30). Informed consent for the therapeutic program was obtained from the relatives of all enrolled children. The study was approved by the Hospital's Ethical Committee. METHODS: All children were given exercise and occupational therapy based on standard treatments. (1) In the control group, the children were additionally treated with routine acupuncture and massage during the one-month basic rehabilitative training. The acupuncture needles were manipulated alternately at the acupoints of Jianyu, Shousanli, Binao, Hegu, Huantiao, Xuanzhong, and Biguan. They were treated once per day, 6 days a week, followed by one day of rest. The children's limbs were first massaged by pressing, stretching, rolling, and kneading. Then, the same acupuncture points were pressed with the thumb for 30 minutes each time, once a day, 6 days a week, followed by one day of rest. The treatment continued for 1 month. (2) In the treatment group, the children were administered acupuncture and massage at the Shu and He points during the one-month basic rehabilitative treatment. For acupuncture of the upper limbs, the following acupoints were used: Sanjian and Quchi of the Large Intestine Meridian of Hand-Yangming, and Houxi and Xiaohai of the Small Intestine Meridian of Hand-Taiyang. The acupoints were acupunctured alternately every other day. For acupuncture of the lower limbs, the following acupoints were used; Zulinqi and Yanglingquan of the Gall bladder Meridian of Foot-Shaoyang, Taibai and Yinlingquan of the Spleen Meridian of Foot-Taiyang, or Xiangu and Zusanli of the Stomach Meridian of Foot-Yangming. The acupoints were acupunctured alternately every other day. Three needles were for the medial malleolus and three needles for the lateral malleolus. They were treated once a day, 6 days a week, followed by one day of rest. The children's limbs were first massaged by means of pressing, stretching, rolling, and kneading. The same acupuncture points were then pressed with the thumb for 0.5-1 minute at each point, 30 minutes each time, once a day, 6 days a week, followed by one day of rest. MAIN OUTCOME MEASURES: Therapeutic efficacy was evaluated using gross motor function measurements, as well as activity of daily living scale before and after treatment. RESULTS: All 60 enrolled children with spastic cerebral palsy were included in the final analysis of results. (1) Comparison of gross motor function scores: The gross motor function score after treatment was significantly higher in both the treatment group and control group than before treatment (t = -3.86 and -8.882, respectively, P 〈 0.05). The scores in the treatment group were significantly higher than the control group scores (t = -7.166, P 〈 0.01). (2) Changes in activity of daily living scores: The scores for activity of daily living in the treatment group was significantly higher after treatment than before treatment (t = -3.933, P 〈 0.01), and was also significantly higher than the control group (t = -0.671, P 〈 0.05). CONCLUSION: Acupuncture and massage at the Shu and He acupoints can significantly ameliorate motor function deficits and movement disabilities in children with spastic cerebral palsy. The therapeutic efficacy at these acupoints is better than routine acupuncture and massage.展开更多
Experiments were carried out on rats anaesthetized with uraethane. The sponta-neous discharges and nociceptive responses of convergent neurons in the right trigerninal nucleus cau-dalis(TNC) to noxious stimuli at rece...Experiments were carried out on rats anaesthetized with uraethane. The sponta-neous discharges and nociceptive responses of convergent neurons in the right trigerninal nucleus cau-dalis(TNC) to noxious stimuli at receptive field (cheek) were recorded extracellularly with glass mi-cro-electrode. Electroacupuncture (EA ) was applied at bilateral " Xiaguan" (ST 7 on face ) or "Zusanli" (ST 36 on shank) acupoint with Iow (2V) and high (18V) intensity. The noclceptive re-sponse of convergent neurons in TNC could be inhihited by low intensity EA applied at "Xiaguan" butnot "Zusanlil", showing the specificity of acupoints. High intensity EA at either "Xiaguan" or "Zusan-li" also reduced the nociceptive responses, showing the analgesic extensiveness of acupoints. We sug-gest that "the gate of control" mechanism plays a main role in low intensity EA and "diffuse noxiousinhibitory controls" (DNIC) rnechanism does so in high intensity EA.The results suggest that we should pay attention to the location of acupoints,展开更多
In this paper, 148 cases of cerebral infarction were treated with acupuncture at acupoints of Governor meridian (treatment group), and the other 97 cases were treated simultaneously with conventional body acupuncture ...In this paper, 148 cases of cerebral infarction were treated with acupuncture at acupoints of Governor meridian (treatment group), and the other 97 cases were treated simultaneously with conventional body acupuncture (control group). The results show that the marked improvement rate was 42. 6 % and the total effective rate was 96. 6 % in the treatment group; while in the control group, the marked improvement rate and the total effective rate were 28. 9 % and 89. 1 % respectively.It suggests that the therapeutic effect in the treatment group is better than that of the control group.The roles of regulation and ameliorating hemorheology of acupuncture at acupoints of Governor meridian are the foundation of its treatment mechanism.展开更多
In this article, the concentration of calcium ion(Ca<sup>21</sup> ) in rabbits’ acupoints hasbeen observed in vivo by means of the Ca<sup>21</sup> selective acupuncture electrode (Ca<sup>...In this article, the concentration of calcium ion(Ca<sup>21</sup> ) in rabbits’ acupoints hasbeen observed in vivo by means of the Ca<sup>21</sup> selective acupuncture electrode (Ca<sup>21</sup> SAE). We discov-ered that stimulating the acupoints in the meridian could cause the Ca<sup>21</sup> concentration increasing inother points of the same meridian but reducing in the control points (lateral to the meridian points).The results indicate that Ca<sup>21</sup> may involve in the activities of the meridians.展开更多
Objective:To explore the function of cluster needling at scalp points therapy on regulating differential protein's expression at different time points in middle cerebral artery occlusion(MCAO)model rats.Methods:Fi...Objective:To explore the function of cluster needling at scalp points therapy on regulating differential protein's expression at different time points in middle cerebral artery occlusion(MCAO)model rats.Methods:Fifty-four rats were divided into three groups randomly and 18 rats in each group.The groups respectively were the model group(group M,n=18),cluster needling at scalp points group(group C,n=18),false operation group(group F,n=18).Each group was then assigned in three subgroups,including 24-h,7-day,and 14-day subgroups.Six rats in each subgroup.Acupuncture at Baihui(GV20)and 2 points beside Baihui,which was 3 e4 mm away from the midline.Longa score was used to evaluated neurological effects.Proteomics methods were used to identify differentially expression proteins with a standard of fold change greater than 1.5 and P<.05 at different times.Results:1.Nerve function scoring:The nerve function scores at 7 and 14 days decreased in group C,which showed better neural function than group M(P<.05).2.Fold change in proteins:Group M showed932 differentially expressed proteins compared with group F,and among them,414 proteins showed significant changes in expression after acupuncture.The expression levels of Cdc42 and GFAP were increased,and Mag,Shank2,and MBP levels were decreased.In the Gene Ontology analysis,the cellular component consisted of the terms cytoplasm,cytoskeleton,lysosome,and plasma membrane.The main related biological processes were cellecell signaling,protein transport,aging,and cell adhesion.Many synaptic and metabolic pathways were found by KEGG analysis.Conclusion:Cluster needling at scalp acupoints can improve the nerve function score and improve dyskinesia in MCAO model rats.Cluster needling at scalp acupoints can regulate the expression of 414 proteins,including Cdc42,GFAP,Mag,Shank2,and MBP,which are related to cerebral ischemia.The differential proteins are major concentration in cytoplasm,cytoskeleton,lysosomes,and plasma membrane,participate in cellecell signaling,protein transport,aging,and cell adhesion,and act through multiple synaptic and metabolic pathways to exert their biological functions.展开更多
Background: It is an urgent matter to relieve the side effects induced by chemotherapy. In recent years, auricular acupoints therapy, which is a nursing technique used in traditional Chinese medicine (TCM), has bee...Background: It is an urgent matter to relieve the side effects induced by chemotherapy. In recent years, auricular acupoints therapy, which is a nursing technique used in traditional Chinese medicine (TCM), has been used on many patients who are undergoing chemotherapy without causing any side effects. Furthermore, it has a good efficacy and is inexpensive. Aim: The aim of this study was to provide evidence for the need of further research in the treatment and nursing of patients during chemotherapy. Methods: We conducted an integrated review of the reports in CNK1, WanFang Data, SinoMed, and PubMed databases for journal articles published after 1990. The search terms included auricular acupoints taping and pressing, auricular acupoints therapy, auricular point sticking, ear points, chemotherapy, and side effects and relevant Medical Subject Heading terms. We then analyzed the mechanism, acu- points, and operation method and summarized the application of auricular acupoints therapy in patients suffering from chemotherapy side effects. Results: This review included 102 articles published between 1990 and 2016, and we brought 63 other articles into the study. Eighteen articles focused on auricular acupoints therapy, combined with other therapy, to relieve patient side effects during chemotherapy. Conclusions: Auricular acupoints therapy can prevent and relieve the side effects induced by chemotherapy. It deserves further clinical applications.展开更多
Backgroud:Clinical studies on acupuncture treatment of hyperplasia of mammary gland(HMG)have proved its effectiveness,but most studies have paid little attention to acupoints prescription and acupoint compatibility.Th...Backgroud:Clinical studies on acupuncture treatment of hyperplasia of mammary gland(HMG)have proved its effectiveness,but most studies have paid little attention to acupoints prescription and acupoint compatibility.The clinical prescription is not identical,the curative effect also has the difference.Therefore,through data mining and network analysis,this study explored the core acupoints and the compatibility law of acupoints in acupuncture treatment of HMG.Methods:To search and select qualified literature according to inclusion and exclusion criteria for relevant clinical research literature on acupuncture treatment of HMG in CNKI,VIP database,WanFang database and PubMed,etc.Then extract relevant information and establish a database.Using the method of statistical and complex network analysis,this paper studies the core acupoints and the law of acupoint compatibility.Results:A total of 104 Chinese literatures and 0 English literatures were included and 106 acupuncture prescriptions were extracted.The core acupoints in the treatment of HMG are Danzhong(CV 17),Wuyi(ST 15),Zusanli(ST 36),Jianjing(GB 21).Danzhong(CV 17)and Zusanli(ST 36),Danzhong(CV 17)and Wuyi(ST 15),Jianjing(GB 21)and Tianzong(SI 11),Jianjing(GB 21)and Wuyi(ST 15)have the highest correlation degree.The method of acupoint matching mainly consists of local-remote acupoints,upper-lower acupoints and front-rear acupoints.Conclusion:The results of a network analysis substantially accord with the general rules of acupuncture theories in traditional Chinese medicine,able to reflect the points-selection principles and features in acupuncture treatment of HMG and provide evidence for the acupoints selection in the treatment of HMG in acupuncture clinic.展开更多
With the help of computers,we have made statistics as to the frequency of se-lecting points for the eight categories of patterns of imbalance.We suggest that for the heat syn-drome,the points in the upper part should ...With the help of computers,we have made statistics as to the frequency of se-lecting points for the eight categories of patterns of imbalance.We suggest that for the heat syn-drome,the points in the upper part should usually be selected,for the cold syndrome,the points inthe lower part should generally be adopted;for the exterior syndrome,the points on the yang surfaceshould mostly be selected,for the interior syndrome,the points on the yin surface should generally beadopted;for the asthenic syndrome,the points in the trunk should mostly be selected,for the sthenicsyndrome,the points at the extremities generally be adopted.展开更多
This paper discusses the selection of abdominal area kidney and meridian acupoints120 patients suffering from lower lumbar pain using the fingers to press the acupoints. Althoughthe types of ailments, mildness or seve...This paper discusses the selection of abdominal area kidney and meridian acupoints120 patients suffering from lower lumbar pain using the fingers to press the acupoints. Althoughthe types of ailments, mildness or severity of the patients’ conditions f and duration of illness var-ied, in all cases satisfactory treatment results were achieved. The average effective rate was 97.5%. The average rate of cure (complete recovery rate) was 73.3%. Patients who had sufferedfrom symptoms for a period of less than one year and patients afflicted with mild or moderate con-ditions enjoyed the most outstanding treatment results. The advantages of this treatment method or technique are as follows: 1. This method ortechnique brings the distinctive dynamics of traditional Chinese medicine theory and meridian the-ory into full play. 2. This method of treatment uses neither acupuncture needles nor moxibus-tion. It merely utilizes the fingers to press certain acupoints. It is simple, convenient and easy toimplement.It is展开更多
Differentiation of syndromes can be done by three steps:First to differentiate thenature of disease,second to differentiate the location of disease,third to analyse comprehensively thenature and location of disease.De...Differentiation of syndromes can be done by three steps:First to differentiate thenature of disease,second to differentiate the location of disease,third to analyse comprehensively thenature and location of disease.Depend on complete differentiation of syndromes,the acupoints are selected.Three cases are analysed to explain how to dominate preceding contents.展开更多
98 patients with sciatica are treated.by stimubting acupoints using a self-made electro-thermal moxibustion instrument.Results show that 82 cases arecured,8 improved and 5 effective,with the total effective rate of 9...98 patients with sciatica are treated.by stimubting acupoints using a self-made electro-thermal moxibustion instrument.Results show that 82 cases arecured,8 improved and 5 effective,with the total effective rate of 96.9%.The temperature relesed from this instrument is adjustable.The electro-thermal moxibustion therapy is short in treatment duration and rapid in taking effect.Ithas no needling-induced pain and no side-effects,and is accepted preferably by patients.展开更多
At present, the specificity of meridians and acupoints has been studied using functional brain imaging techniques from many standpoints, including meridians, acupoints, and sham acupoints, as well as different meridia...At present, the specificity of meridians and acupoints has been studied using functional brain imaging techniques from many standpoints, including meridians, acupoints, and sham acupoints, as well as different meridians and acupoints, coordination of acupoints, and factors influencing meridian and acupoint specificity Preliminary experimental data have demonstrated that acupuncture at meridians and acupoints is specific with regard to brain neural information. However, research findings are contradictory, which may be related to brain functional complexity, resolution of functional brain imaging techniques, and experimental design. Future studies should further improve study method, and should strictly control experimental conditions to better analyze experimental data and acquire more beneficial data. Because of its many advantages, the functional brain imaging technique is a promising method for studying meridian and acupoint specificity.展开更多
Disorder of consciousness (DOC) is one of the most serious sequelae of brain injury, and is challenging for neurologists and rehabilitation special- ists to manage because of its refractory nature (Whyte et al., 2...Disorder of consciousness (DOC) is one of the most serious sequelae of brain injury, and is challenging for neurologists and rehabilitation special- ists to manage because of its refractory nature (Whyte et al., 2013). Acu- puncture is a traditional Chinese medicine technique that is often used to help improve the level of consciousness in patients with DOC. However, the responses to stimulation of acupoints in patients with DOC are not fully understood.展开更多
[Objectives] This study aimed to explore the effects of moxibustion at the Yanglingquan and Yinlingquan points on thickness of the left foot and synovial tissue inflammation of collagen-induced arthritis( CIA) rats,an...[Objectives] This study aimed to explore the effects of moxibustion at the Yanglingquan and Yinlingquan points on thickness of the left foot and synovial tissue inflammation of collagen-induced arthritis( CIA) rats,and to explore the therapeutic effect of moxibustion at complementary acupoints on rheumatoid arthritis( RA). [Methods] Total 32 Wistar rats were selected. Among them,8 rats were selected randomly using the randomized digital method as the blank control( K),and the remaining were subjected to modeling. After the successful establishment of the CIA model,the 24 rats were randomly and evenly divided into model group( M),Yanglingquan and Yinlingquan group( AB) and Shenshu and Zusanli group( CD). The rats in the moxibustion groups were subjected to bilateral synchronous moxibustion once every two days( 2 mg refined moxa/cone,5 cones/( point·d)),for total 10 times. The left plantar thickness of each rat was measured before modeling,after modeling,and after the last intervention,respectively. After the last intervention,the situation of synovial tissue inflammation in each rat of the treatment group was inspected. [Results] Compared with that of the control group,the left foot thickness of each group of rats that were modeled all increased significantly( P < 0. 05). And the synovial tissue showed different degrees of inflammatory infiltration,fibrous hyperplasia,and angiogenes. After the intervention ended,compared with that in the M group,the left foot thickness in the AB and CD treatment groups reduced significantly( P < 0. 05). The inflammatory infiltration,fibrous hyperplasia,and angiogenes of the synovial tissue decreased. Between the two moxibustion groups,the thickness of the left foot in group AB decreased compared with group CD( P < 0. 05). The inflammatory infiltration,fibrous hyperplasia and angiogenes in group AB decreased compared with those in group CD. [Conclusions] Moxibustion at the complementary acupoints of Yanglingquan and Yinlingquan,Shenshu and Zusanli both showed good improvement effect on CIA rats. In terms of left foot thickness and synovial tissue inflammation,the treatment group AB was superior to the group CD,and the specific mechanism needs further study.展开更多
Using Psychopysical method 126 receptive units in 36 acupoints at human limbs wereclassified.It was shown that the groups of the receptive units were related closely to their peripheralanatomical structures.
Acupuncture at certain acupoints (specific sites for needle insertion) can induce the activation of specific regions of the brain. This activated brain region influences the target organ or tissue in order to treat ...Acupuncture at certain acupoints (specific sites for needle insertion) can induce the activation of specific regions of the brain. This activated brain region influences the target organ or tissue in order to treat the disease. Acupoint specificity is the basis of acupuncture therapy.展开更多
基金Natural Science Foundation of Shanxi Province(No.201801D121358)"Basic Research Special Project"of Shanxi University of Tradit Chin Med Science and Technology Innovation Ability Cultivation Program(No.2021PY-JC-13)。
文摘Objective:To investigate the mechanism of JAK2/STAT3/SOCS1 signaling pathway in electroacupuncture of different acupoints on acute colitis rats.Methods:36 SPF SD rats were randomly divided into 6 groups,with 6 rats in each group.The rat model of acute colitis was prepared by enema with glacial acetic acid solution.After the model was established,electroacupuncture was given to each acupoint group,with density wave,frequency 2Hz-50 Hz,intensity 2 mA,muscle tremor as the degree 20 min/time,1 time/day,for 3 consecutive days.Observe the general condition of rats;the pathological changes of colonic mucosa in rats were observed by HE method.The contents of serum interleukin-4(IL-4)and interleukin-8(IL-8)were detected by ELISA.Western blot and RT-PCR were used to detect the expression of JAK2,STAT3,SOCS1 protein and mRNA in rat colon tissue.Results:In contrast to the normal group,the overall condition of the model group was worse,the colonic mucosa was severely damaged,even necrotic,and the ulcer surface was obvious.The content of IL-4 in serum was obviously reduced,and the content of IL-8 was obviously go up(P<0.01).The protein content of JAK2,STAT3 and the expression of JAK2,STAT3 mRNA in colon tissue of rats were obviously go up,while the protein content of SOCS1 and the expression of SOCS1 mRNA were obviously reduced(P<0.01).In contrast to the model group,the general condition of rats in each acupoint group was significantly improved,the damage and necrosis of colonic mucosa and ulcer surface were obviously alleviated,the content of IL-4 in serum was obviously go up,and the content of IL-8 was significantly decreased(P<0.01).The protein content of JAK2,STAT3 and the expression of JAK2,STAT3 mRNA in colon tissue of rats were obviously reduced,while the protein content of SOCS1 and the expression of SOCS1 mRNA were obviously go up(P<0.05,P<0.01).Comparison of different acupoint groups,the colonic mucosal injury in the Zusanli group was significantly reduced,the content of serum IL-4 was significantly increased,and the content of IL-8 was significantly decreased(P<0.05,P<0.01).The protein content and mRNA expression of JAK2 and STAT3 in colon tissue were significantly down-regulated,while the protein content and mRNA expression of SOCS1 were significantly go up(P<0.05,P<0.01).Conclusion:Electroacupuncture at each acupoint can improve the damage of colonic mucosa and reduce the inflammatory response.The therapeutic effect of Zusanli(ST36)is better than that of Tianshu(ST25),Dachangshu(BL25)and Shangjuxu(ST37).The mechanism may be related to the regulation of JAK2/STAT3/SOCS1 signaling pathway related proteins and inflammatory cytokines IL-4 and IL-8.
基金supported by the Guangdong Province Science and Technology Plan,No.2010B030700008the Guangzhou Science and Technology Plan Application Foundation,No.2012J4300062the Major Project of Science and Technology Plan of Dongguan City in 2012,No.2012105102022
文摘Peripheral nerve injury not only affects the site of the injury, but can also induce neuronal apop- tosis at the spinal cord. However, many acupuncture clinicians still focus only on the injury site, selecting acupoints entirely along the injured nerve trunk and neglecting other regions; this may delay onset of treatment efficacy and rehabilitation. Therefore, in the present study, we compared the clinical efficacy of acupuncture at Governor vessel and local meridian acupoints combined (GV/LM group) with acupuncture at local meridian acupoints alone (LM group) in the treatment of patients with peripheral nerve injury. In the GV/LM group (n = 15), in addition to meridian acupoints at the injury site, the following acupoints on the Governor vessel were stimulated: Baihui (GV20), Fengfu (GV16), Dazhui (GV14), and Shenzhu (GV12), selected to treat nerve injury of the upper limb, and Jizhong (GV6), Mingmen (GV4), Yaoyangguan (GV3), and Yaoshu (GV2) to treat nerve injury of the lower limb. In the LM group (n = 15), only me- ridian acupoints along the injured nerve were selected. Both groups had electroacupuncture treatment for 30 minutes, once a day, 5 times per week, for 6 weeks. Two cases dropped out of the LM group. A good or excellent clinical response was obtained in 80% of the patients in the GV/ LM group and 38.5% of the LM group. In a second study, an additional 20 patients underwent acupuncture with the same prescription as the GV/LM group. Electomyographic nerve conduc- tion tests were performed before and after acupuncture to explore the mechanism of action of the treatment. An effective response was observed in 80.0% of the patients, with greater motor nerve conduction velocity and amplitude after treatment, indicating that electroacupuncture on specific Governor vessel acupoints promotes functional motor nerve repair after peripheral nerve injury. In addition, electromyography was performed before, during and after electroacu- puncture in one patient with radial nerve injury. After a single session, the patient's motor nerve conduction velocity increased by 23.2%, indicating that electroacupuncture at Governor vessel acupoints has an immediate therapeutic effect on peripheral nerve injury. Our results indicate that Governor vessel and local meridian acupoints used simultaneously promote functional repair after peripheral nerve injury. The mechanism of action may arise from an improvement of the local microenvironment in injured nervous tissue, as well as immediate effects of Governor vessel and local meridian acupoint stimulation to ensure the continuity between the peripheral and central nervous systems.
基金supported by the National Natural Science Foundation of China,No.14202225,&.81574042the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,No.2000202a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,No.JDZX2015314
文摘Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui(GV14),Jizhong(GV6),Houhui,Guanyuan(CV4)and Zhongwan(CV12).The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter(GLAST)and glial glutamate transporter-1(GLT-1)expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured(left)side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
基金the Grants from State Administration of Traditional Chinese Medicine of the People's Republic of China, No.Z1200313
文摘BACKGROUND: Based on clinical findings and syndromes, it has been previously shown that therapeutic methods for cerebral palsy can be greatly improved by selecting fewer, but more accurate acupoints. Therefore, greater therapeutic efficacy for cerebral palsy could be obtained by selecting the five Shu acupoints, which are located at the qi-intersecting point of the twelve meridians below the elbow and knee. OBJECTIVE: To treat children with spastic cerebral palsy using acupuncture and massage at the Shu and He points through a combination of rehabilitative training, and to compare the effects with routine acupuncture and massage. DESIGN: A randomized controlled clinical trial. SETTING: Rehabilitation Center for Cerebral Palsy, Department of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine. PARTICIPANTS: Sixty children with spastic cerebral palsy were selected from the Rehabilitation Center for Cerebral Palsy, Department of Pediatrics, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from May 2003 to February 2006. There were 38 boys and 22 girls, aged 10 months to 4 years old, muscle strength ranging from grade I to grade IV. The children were randomly divided into a treatment group (n =30) and a control group (n =30). Informed consent for the therapeutic program was obtained from the relatives of all enrolled children. The study was approved by the Hospital's Ethical Committee. METHODS: All children were given exercise and occupational therapy based on standard treatments. (1) In the control group, the children were additionally treated with routine acupuncture and massage during the one-month basic rehabilitative training. The acupuncture needles were manipulated alternately at the acupoints of Jianyu, Shousanli, Binao, Hegu, Huantiao, Xuanzhong, and Biguan. They were treated once per day, 6 days a week, followed by one day of rest. The children's limbs were first massaged by pressing, stretching, rolling, and kneading. Then, the same acupuncture points were pressed with the thumb for 30 minutes each time, once a day, 6 days a week, followed by one day of rest. The treatment continued for 1 month. (2) In the treatment group, the children were administered acupuncture and massage at the Shu and He points during the one-month basic rehabilitative treatment. For acupuncture of the upper limbs, the following acupoints were used: Sanjian and Quchi of the Large Intestine Meridian of Hand-Yangming, and Houxi and Xiaohai of the Small Intestine Meridian of Hand-Taiyang. The acupoints were acupunctured alternately every other day. For acupuncture of the lower limbs, the following acupoints were used; Zulinqi and Yanglingquan of the Gall bladder Meridian of Foot-Shaoyang, Taibai and Yinlingquan of the Spleen Meridian of Foot-Taiyang, or Xiangu and Zusanli of the Stomach Meridian of Foot-Yangming. The acupoints were acupunctured alternately every other day. Three needles were for the medial malleolus and three needles for the lateral malleolus. They were treated once a day, 6 days a week, followed by one day of rest. The children's limbs were first massaged by means of pressing, stretching, rolling, and kneading. The same acupuncture points were then pressed with the thumb for 0.5-1 minute at each point, 30 minutes each time, once a day, 6 days a week, followed by one day of rest. MAIN OUTCOME MEASURES: Therapeutic efficacy was evaluated using gross motor function measurements, as well as activity of daily living scale before and after treatment. RESULTS: All 60 enrolled children with spastic cerebral palsy were included in the final analysis of results. (1) Comparison of gross motor function scores: The gross motor function score after treatment was significantly higher in both the treatment group and control group than before treatment (t = -3.86 and -8.882, respectively, P 〈 0.05). The scores in the treatment group were significantly higher than the control group scores (t = -7.166, P 〈 0.01). (2) Changes in activity of daily living scores: The scores for activity of daily living in the treatment group was significantly higher after treatment than before treatment (t = -3.933, P 〈 0.01), and was also significantly higher than the control group (t = -0.671, P 〈 0.05). CONCLUSION: Acupuncture and massage at the Shu and He acupoints can significantly ameliorate motor function deficits and movement disabilities in children with spastic cerebral palsy. The therapeutic efficacy at these acupoints is better than routine acupuncture and massage.
文摘Experiments were carried out on rats anaesthetized with uraethane. The sponta-neous discharges and nociceptive responses of convergent neurons in the right trigerninal nucleus cau-dalis(TNC) to noxious stimuli at receptive field (cheek) were recorded extracellularly with glass mi-cro-electrode. Electroacupuncture (EA ) was applied at bilateral " Xiaguan" (ST 7 on face ) or "Zusanli" (ST 36 on shank) acupoint with Iow (2V) and high (18V) intensity. The noclceptive re-sponse of convergent neurons in TNC could be inhihited by low intensity EA applied at "Xiaguan" butnot "Zusanlil", showing the specificity of acupoints. High intensity EA at either "Xiaguan" or "Zusan-li" also reduced the nociceptive responses, showing the analgesic extensiveness of acupoints. We sug-gest that "the gate of control" mechanism plays a main role in low intensity EA and "diffuse noxiousinhibitory controls" (DNIC) rnechanism does so in high intensity EA.The results suggest that we should pay attention to the location of acupoints,
文摘In this paper, 148 cases of cerebral infarction were treated with acupuncture at acupoints of Governor meridian (treatment group), and the other 97 cases were treated simultaneously with conventional body acupuncture (control group). The results show that the marked improvement rate was 42. 6 % and the total effective rate was 96. 6 % in the treatment group; while in the control group, the marked improvement rate and the total effective rate were 28. 9 % and 89. 1 % respectively.It suggests that the therapeutic effect in the treatment group is better than that of the control group.The roles of regulation and ameliorating hemorheology of acupuncture at acupoints of Governor meridian are the foundation of its treatment mechanism.
文摘In this article, the concentration of calcium ion(Ca<sup>21</sup> ) in rabbits’ acupoints hasbeen observed in vivo by means of the Ca<sup>21</sup> selective acupuncture electrode (Ca<sup>21</sup> SAE). We discov-ered that stimulating the acupoints in the meridian could cause the Ca<sup>21</sup> concentration increasing inother points of the same meridian but reducing in the control points (lateral to the meridian points).The results indicate that Ca<sup>21</sup> may involve in the activities of the meridians.
基金National Natural Science Foundation of China(No.81473775)。
文摘Objective:To explore the function of cluster needling at scalp points therapy on regulating differential protein's expression at different time points in middle cerebral artery occlusion(MCAO)model rats.Methods:Fifty-four rats were divided into three groups randomly and 18 rats in each group.The groups respectively were the model group(group M,n=18),cluster needling at scalp points group(group C,n=18),false operation group(group F,n=18).Each group was then assigned in three subgroups,including 24-h,7-day,and 14-day subgroups.Six rats in each subgroup.Acupuncture at Baihui(GV20)and 2 points beside Baihui,which was 3 e4 mm away from the midline.Longa score was used to evaluated neurological effects.Proteomics methods were used to identify differentially expression proteins with a standard of fold change greater than 1.5 and P<.05 at different times.Results:1.Nerve function scoring:The nerve function scores at 7 and 14 days decreased in group C,which showed better neural function than group M(P<.05).2.Fold change in proteins:Group M showed932 differentially expressed proteins compared with group F,and among them,414 proteins showed significant changes in expression after acupuncture.The expression levels of Cdc42 and GFAP were increased,and Mag,Shank2,and MBP levels were decreased.In the Gene Ontology analysis,the cellular component consisted of the terms cytoplasm,cytoskeleton,lysosome,and plasma membrane.The main related biological processes were cellecell signaling,protein transport,aging,and cell adhesion.Many synaptic and metabolic pathways were found by KEGG analysis.Conclusion:Cluster needling at scalp acupoints can improve the nerve function score and improve dyskinesia in MCAO model rats.Cluster needling at scalp acupoints can regulate the expression of 414 proteins,including Cdc42,GFAP,Mag,Shank2,and MBP,which are related to cerebral ischemia.The differential proteins are major concentration in cytoplasm,cytoskeleton,lysosomes,and plasma membrane,participate in cellecell signaling,protein transport,aging,and cell adhesion,and act through multiple synaptic and metabolic pathways to exert their biological functions.
文摘Background: It is an urgent matter to relieve the side effects induced by chemotherapy. In recent years, auricular acupoints therapy, which is a nursing technique used in traditional Chinese medicine (TCM), has been used on many patients who are undergoing chemotherapy without causing any side effects. Furthermore, it has a good efficacy and is inexpensive. Aim: The aim of this study was to provide evidence for the need of further research in the treatment and nursing of patients during chemotherapy. Methods: We conducted an integrated review of the reports in CNK1, WanFang Data, SinoMed, and PubMed databases for journal articles published after 1990. The search terms included auricular acupoints taping and pressing, auricular acupoints therapy, auricular point sticking, ear points, chemotherapy, and side effects and relevant Medical Subject Heading terms. We then analyzed the mechanism, acu- points, and operation method and summarized the application of auricular acupoints therapy in patients suffering from chemotherapy side effects. Results: This review included 102 articles published between 1990 and 2016, and we brought 63 other articles into the study. Eighteen articles focused on auricular acupoints therapy, combined with other therapy, to relieve patient side effects during chemotherapy. Conclusions: Auricular acupoints therapy can prevent and relieve the side effects induced by chemotherapy. It deserves further clinical applications.
文摘Backgroud:Clinical studies on acupuncture treatment of hyperplasia of mammary gland(HMG)have proved its effectiveness,but most studies have paid little attention to acupoints prescription and acupoint compatibility.The clinical prescription is not identical,the curative effect also has the difference.Therefore,through data mining and network analysis,this study explored the core acupoints and the compatibility law of acupoints in acupuncture treatment of HMG.Methods:To search and select qualified literature according to inclusion and exclusion criteria for relevant clinical research literature on acupuncture treatment of HMG in CNKI,VIP database,WanFang database and PubMed,etc.Then extract relevant information and establish a database.Using the method of statistical and complex network analysis,this paper studies the core acupoints and the law of acupoint compatibility.Results:A total of 104 Chinese literatures and 0 English literatures were included and 106 acupuncture prescriptions were extracted.The core acupoints in the treatment of HMG are Danzhong(CV 17),Wuyi(ST 15),Zusanli(ST 36),Jianjing(GB 21).Danzhong(CV 17)and Zusanli(ST 36),Danzhong(CV 17)and Wuyi(ST 15),Jianjing(GB 21)and Tianzong(SI 11),Jianjing(GB 21)and Wuyi(ST 15)have the highest correlation degree.The method of acupoint matching mainly consists of local-remote acupoints,upper-lower acupoints and front-rear acupoints.Conclusion:The results of a network analysis substantially accord with the general rules of acupuncture theories in traditional Chinese medicine,able to reflect the points-selection principles and features in acupuncture treatment of HMG and provide evidence for the acupoints selection in the treatment of HMG in acupuncture clinic.
文摘With the help of computers,we have made statistics as to the frequency of se-lecting points for the eight categories of patterns of imbalance.We suggest that for the heat syn-drome,the points in the upper part should usually be selected,for the cold syndrome,the points inthe lower part should generally be adopted;for the exterior syndrome,the points on the yang surfaceshould mostly be selected,for the interior syndrome,the points on the yin surface should generally beadopted;for the asthenic syndrome,the points in the trunk should mostly be selected,for the sthenicsyndrome,the points at the extremities generally be adopted.
文摘This paper discusses the selection of abdominal area kidney and meridian acupoints120 patients suffering from lower lumbar pain using the fingers to press the acupoints. Althoughthe types of ailments, mildness or severity of the patients’ conditions f and duration of illness var-ied, in all cases satisfactory treatment results were achieved. The average effective rate was 97.5%. The average rate of cure (complete recovery rate) was 73.3%. Patients who had sufferedfrom symptoms for a period of less than one year and patients afflicted with mild or moderate con-ditions enjoyed the most outstanding treatment results. The advantages of this treatment method or technique are as follows: 1. This method ortechnique brings the distinctive dynamics of traditional Chinese medicine theory and meridian the-ory into full play. 2. This method of treatment uses neither acupuncture needles nor moxibus-tion. It merely utilizes the fingers to press certain acupoints. It is simple, convenient and easy toimplement.It is
文摘Differentiation of syndromes can be done by three steps:First to differentiate thenature of disease,second to differentiate the location of disease,third to analyse comprehensively thenature and location of disease.Depend on complete differentiation of syndromes,the acupoints are selected.Three cases are analysed to explain how to dominate preceding contents.
文摘98 patients with sciatica are treated.by stimubting acupoints using a self-made electro-thermal moxibustion instrument.Results show that 82 cases arecured,8 improved and 5 effective,with the total effective rate of 96.9%.The temperature relesed from this instrument is adjustable.The electro-thermal moxibustion therapy is short in treatment duration and rapid in taking effect.Ithas no needling-induced pain and no side-effects,and is accepted preferably by patients.
基金Major State Basic Research Development Program of China (973 Program), No.2006CB504501
文摘At present, the specificity of meridians and acupoints has been studied using functional brain imaging techniques from many standpoints, including meridians, acupoints, and sham acupoints, as well as different meridians and acupoints, coordination of acupoints, and factors influencing meridian and acupoint specificity Preliminary experimental data have demonstrated that acupuncture at meridians and acupoints is specific with regard to brain neural information. However, research findings are contradictory, which may be related to brain functional complexity, resolution of functional brain imaging techniques, and experimental design. Future studies should further improve study method, and should strictly control experimental conditions to better analyze experimental data and acquire more beneficial data. Because of its many advantages, the functional brain imaging technique is a promising method for studying meridian and acupoint specificity.
基金supported by the National Natural Science Foundation of China,No.81171852
文摘Disorder of consciousness (DOC) is one of the most serious sequelae of brain injury, and is challenging for neurologists and rehabilitation special- ists to manage because of its refractory nature (Whyte et al., 2013). Acu- puncture is a traditional Chinese medicine technique that is often used to help improve the level of consciousness in patients with DOC. However, the responses to stimulation of acupoints in patients with DOC are not fully understood.
基金Supported by Natural Science Foundation of Hebei Province(H2014406047)Natural Science Project of Chengde Medical University(201812)
文摘[Objectives] This study aimed to explore the effects of moxibustion at the Yanglingquan and Yinlingquan points on thickness of the left foot and synovial tissue inflammation of collagen-induced arthritis( CIA) rats,and to explore the therapeutic effect of moxibustion at complementary acupoints on rheumatoid arthritis( RA). [Methods] Total 32 Wistar rats were selected. Among them,8 rats were selected randomly using the randomized digital method as the blank control( K),and the remaining were subjected to modeling. After the successful establishment of the CIA model,the 24 rats were randomly and evenly divided into model group( M),Yanglingquan and Yinlingquan group( AB) and Shenshu and Zusanli group( CD). The rats in the moxibustion groups were subjected to bilateral synchronous moxibustion once every two days( 2 mg refined moxa/cone,5 cones/( point·d)),for total 10 times. The left plantar thickness of each rat was measured before modeling,after modeling,and after the last intervention,respectively. After the last intervention,the situation of synovial tissue inflammation in each rat of the treatment group was inspected. [Results] Compared with that of the control group,the left foot thickness of each group of rats that were modeled all increased significantly( P < 0. 05). And the synovial tissue showed different degrees of inflammatory infiltration,fibrous hyperplasia,and angiogenes. After the intervention ended,compared with that in the M group,the left foot thickness in the AB and CD treatment groups reduced significantly( P < 0. 05). The inflammatory infiltration,fibrous hyperplasia,and angiogenes of the synovial tissue decreased. Between the two moxibustion groups,the thickness of the left foot in group AB decreased compared with group CD( P < 0. 05). The inflammatory infiltration,fibrous hyperplasia and angiogenes in group AB decreased compared with those in group CD. [Conclusions] Moxibustion at the complementary acupoints of Yanglingquan and Yinlingquan,Shenshu and Zusanli both showed good improvement effect on CIA rats. In terms of left foot thickness and synovial tissue inflammation,the treatment group AB was superior to the group CD,and the specific mechanism needs further study.
文摘Using Psychopysical method 126 receptive units in 36 acupoints at human limbs wereclassified.It was shown that the groups of the receptive units were related closely to their peripheralanatomical structures.
文摘Acupuncture at certain acupoints (specific sites for needle insertion) can induce the activation of specific regions of the brain. This activated brain region influences the target organ or tissue in order to treat the disease. Acupoint specificity is the basis of acupuncture therapy.