APPLICATION OF ACUPUNCTURE ANESTHESIA TO APPENDECTOMY AND COMMENTS ON THE OPERATION AND ITS POSSIBLE MECHANISMS

Objectives: To observe the analgesic effect and to analyze the related mechanisms of acupuncture anesthesia (AA) for appendectomy. Methods: A total of 41 cases of appendicitis patients (24 males and 17 females) were subjected into this observation. Electroacupuncture (AA, 60-100 Hz, a bearable stimulus strength) of Pishu (BL 20), Weishu (BL 21), Zhangmen (LR 13) and Wushu (GB 27) was performed beginning 30 minutes before operation. Adjuvants used were luminal (100 mg, intra muscular injection), dolantin (1 mg/kg), 0.5% procaine (1-2 mL). Results: Of the 41 cases, the successful rate was 87.80% and the excellent plus good rate was 75.61% respectively. Conclusion: Acupuncture anesthesia is applicable for appendectomy in clinic but is still to be improved further in operation.

SURVEY ON CLINICAL STUDY OF COMPOUND ACUPUNCTURE ANESTHESIA IN RECENT 10 YEARS IN CHINA

In the present paper, the authors make a review on the progresses of acupuncture anesthesia (AA) from ① historical development, ② research on acupuncture combined with local anesthesia; ③ research on acupuncture combined with epidural anesthesia; and ④ research on acupuncture combined with general anesthesia. Compound acupuncture anesthesia provides a new anesthetic measure for surgical operations and has a definite analgesic effect and many advantages, and should be investigated further.

The history of acupuncture anesthesia for pneumonectomy in Shanghai during the 1960s

The success of acupuncture anesthesia （AA） for pneumonectomy in Shanghai in 1960 was a key event for AA gaining practical clinical application. The effort was a close collaboration between the Shanghai First Tuberculosis Hospital and the Shanghai Institute of Acupuncture and Moxibustion. One of the most important factors of AA success was the great financial and political support provided by the Chinese central government and Shanghai local government. In December 1965 the State Science and Technology Commission of China issued a formal document acknowledging AA as an important first-leve national achievement of the integration of Chinese and Western medicine, and a collaborative effort of the whole scientific community in China. AA was an important influential factor that helped acupuncture spread across the world.

Effect of acupuncture anesthesia on acne vulgaris of pricking-blood-letting cupping: a single-blind randomized clinical trail

OBJECTIVE: To evaluate the effect on acne vulgaris of pricking-bloodletting cupping at Dazhui(GV 14) under acupuncture anesthesia, and establish whether providing anesthesia to the treatment area by manipulating Hegu(LI 4) and Quchi(LI 11) might have an additional therapeutic benefit. METHODS: Thirty-eight patients were recruited and randomized into a control group and an intervention group with a single-blind(observer-blind) method. The control group was treated by pricking-bloodletting cupping at Dazhui(GV 14) and the studied group by pricking-bloodletting cupping at Dazhui(GV 14) under acupuncture anesthesia at Hegu(LI 4) and Quchi(LI 11). Both groups were treated twice weekly for 6 weeks. The analgesic and therapeutic effects of acupuncture were evaluated on a visual analog scale(VAS) and global acne grading system(GAGS), respectively.RESULTS: There were differences in the VAS scores of pain on pricking and in the pricked area, and the duration of pain between the groups. After 12 treatments, there was a significant reduction in GAGS scores from baseline in both groups, but there was no significant difference between the groups. CONCLUSION: Acupuncture anesthesia at Hegu(LI 4) and Quchi(LI 11) is an effective means of alleviating the pain of pricking-bloodletting cupping and reducing the duration of pain in the treatment area. Pricking-bloodletting cupping at Dazhui(GV 14) improves the skin lesions of patients with moderate acne vulgaris, but acupuncture anesthesia does not appear to have an additional therapeutic effect.

Experience and Thinking on Study of Pneumonectomy by Acupuncture Anesthesia

Acupuncture anesthesia is a great breakthrough in the development history of acupuncture science in China. It has promoted the development of acupuncture science, promoted the study on acupuncture from multiple subjects of modem natural sciences and has become an important component part in the studies of modem life scienceIll. Pneumonectomy under acupuncture anesthesia is an important component part of the research outcomes in acupuncture anesthesia, and the research professionals of Shanghai Lung Hospital and Shanghai Research Institute of Acupuncture and Meridians used to cooperate in the study of pneumonectomy under acupuncture anesthesia and to be in the leading position domestically. This article reviews the research process of pneumonectomy under acupuncture anesthesia, analyzes the issues existing in the study and the difficult points needed to be solved and explores the solving measures and research orientation.

Guiding Role of Muscle Region Theory in Acupuncture Anesthesia

As a part of acupuncture system, acupuncture anesthesia can promote the development of acupuncture theory. Muscle region is also a part of acupuncture system and plays a guiding role in clinical application of acupuncture anesthesia. The correlation between the distribution of muscle regions and nerves acts as the substantial foundation of the guiding role. At the same time, acupuncture anesthesia has been promoting the continuous development of muscle region theory. The cyclic development relation between the two （practice-theory-practice） is consistent with the dialectical development view.

Acupuncture Anesthesia in China:Retrospect and Prospect

Acupuncture treatment is a great creation of ancient physicians in China, and acupuncture analgesia is the most influential therapy. After the founding of the People＇s Republic of China in 1949, the acupuncture analgesia of integrated traditional Chinese and Western medicine has obtained great development.

Meta analysis of application of acupuncture combined with drug anesthesia in anorectal surgery

Objective:To evaluate the effectiveness and safety of acupuncture combined drug anesthesia and simple drug anesthesia in anorectal surgery.Methods:The randomized controlled trials of acupuncture combined with drug anesthesia was evaluated using Cochrane systematic review method.The search scope was China National Knowledge Internet,wanfang database,China Biomedical Literature,Pubmed and Embase.The deadline was December 31,2018.The randomized controlled trials of acupuncture combined with anesthesia that met the inclusion criteria were selected.In terms of trial quality evaluation,Cochrane risk bias was used for evaluation.Finally,RevMan 5.2 software was used for statistical analysis.Results:This study finally included 15 articles,a total of 1321 patients.The heterogeneity of significant rate was good,and the combined effect amount OR was 3.55(95%CI[2.06,6.12]).The combined effect quantitative test(Z=4.57,P<0.00001)showed that there was a statistical significance,indicating that acupuncture combined drug anesthesia was better than simple anesthesia in anorectal surgery.The heterogeneity of adverse reactions was good,and the combined effect test(Z=8.88,P<0.00001)showed that there was a statistical significance,indicating that the adverse reaction rate of acupuncture combined with drug anesthesia was lower than that of simple drug anesthesia in the anorectal surgery.Conclusion:Compared with simple drug anesthesia,acupuncture combined with drug anesthesia can improve the anesthesia effect and reduce the occurrence of adverse reactions in anorectal surgery.

Ear Acupuncture Anesthesia for In Vitro Fertilization and Embryo Transfer Egg Retrieval

Objective：To observe the anesthesia effect of ear acupuncture for in vitro fertilization-embryo transfer （IVF-ET） egg retrieval.Method：All of 160 IVF-ET cases were allocated into a treatment group （120 cases） and a control group （40 cases）.Ear acupuncture and Pethidine Hydrochloride （Dolantin） were employed respectively to alleviate pain.After that,the anesthesia effects in two groups were compared.Results：There were no significant differences in anesthesia effect and retrieved eggs between the treatment group and the control group （P〉0.05）.Conclusion：Ear acupuncture can obtain similar effects as Pethidine Hydrochloride;however,it is safer and more effective.

CLINICAL SUMMARY ON ACUPUNCTURE COMBINED WITH ENFLURANE-INHALATION ANESTHESIA FOR RESECTION OF ESOPHAGEAL CARCINOMA

Objective: To observe the clinical effect of acupuncture combined with general anesthesia by inhaling enflurane for esophageal carcinoma resection. Methods: 120 cases of esophageal carcinoma patients were randomly divided into acupuncture+inhalation of enflurane (A+IE, n=40), electrical stimulation (ES)+IE (n=41) and IE (control, n=39) groups. In all the 3 groups, vecuronium, fentanyl, propofol (same dose) were given intravenously to induce anesthesia before operation, and during operation, fentanyl (2 ug/kg/hr) and vecuronium (0.03 mg/kg/0.5hr) and appropriate concentration of enflurane were given to the patients for maintaining anesthesia and muscular relaxation. In group A, Xiayifeng (below TE 17), Sanyangluo (TE 8) to Ximen (PC 4) were punctured and stimulated electrically, and in group B, these acupoints were stimulated electrically via cutaneous plate electrodes. Results: The anesthetic effect grade I rates of A+IE, ES+IE and IE groups were 67.50%, 68.29% and 28.21% respectively. There was a significant difference between A+IE or ES+IE and IE groups (P<0.001). In the process of anesthesia, the mean concentrations of the inhaled enflurane in A +IE, ES+IE and IE groups were 0.55% and 0.50% and 0.83% separately. The average concentrations of A+IE and ES+IE groups were lower 33.73% and 39.76% than that of group IE (P<0.005). Conclusion: Acupuncture combined with enflurane inhalation can enhance anesthetic effect, reduce the dose of the inhaled enflurane.

SUMMARY OF CLINICAL STUDY ON ACUPUNCTURE COMBINED WITH EPIDURAL ANESTHESIA FOR CHOLECYSTECTOMY

Objective: TO study clinical effect and anesthetic method of acupuncture anesthesia combined with epidural administration of small dose of anesthetic for cholecystectomy. Methods: A total of 194 cases of cholecystectomy patients were randomly divided into acupuncture combined with epidural anesthesia group (group A, n = 66), acupoint-skin electrical stimulation combined with epidural anesthesia group (group B, n = 63) and simple epidural anesthesia group (group C, n=65). Observations were conducted using single-bland method. Bilateral Neiguan (PC 6) and Zusanli (ST 36) were punctured and stimulated electrically in group A and only stimulated electrically via cutaneous electrodes in group B. Epidural anesthetic used was 1.5% Lidocaine and the anesthetic level was controlled to reach T4-11 . Results: The class-I (excellent) rates of group A, B and C were 75.76%, 60.32% and 13.85% respectively, showing significant differences between group A and C and group B and C (P <0.001). The initial dose, doses of every hour and every case of group A and B were lower than those of group C. The dose of every hour of group C was 36.23% and 3. 75% higher then group A and B respectively (P < 0. 001), suggesting that acupuncture or acupoint-skin electrical stimulation could strengthen anesthetic effect and reduce the dose of epidural anesthetic. During operation, indexes of the life signs as HR, MAP, RR, TV, MV, SpO2 and ECG kept basically stable and all patients in group A passed surgical operation safely. Conclusion: Acupuncture or acupoint-skin electrical stimulation combined with epidural anesthesia can be used as one of the anesthetic methods for cholecystectomy.

INFLUENCE OF LOCAL INFILITRATION ANESTHESIA OF SCALP-POINT ON ACUPUNCTURE INDUCED CHANGES OF CEREBRAL BLOOD PERFUSION DETECTED BY SPECT

Objective: To observe the influence of local infiltration anesthesia at the scalp point on acupuncture stimulation induced changes of cerebral blood perfusion in the brain. Methods: Experiments were conducted in 10 healthy volunteer subjects (8 males and 2 females) who were ordered to take a lying position on a specific bed with their heads keeping in a fixed position. Scalp point used was Motor Area (MS 6). The first syringe needle (gauge 5) was inserted into the scalp from the upper 1/5 of MS 6 and the second syringe needle inserted into the scalp from the middle 2/5 of MS 6 and advanced downward, with the two needles connected to a HAN’s Therapeutic Apparatus. Images of the brain were then taken before and after electroacupuncture (EA) stimulation, and after local injection of 1% lidocaine [mixed with epinephrine (1∶200,000, 3 mL)] plus EA by using Siemens ECAM/ICON Single Photo Emission Computed Tomography (SPECT). Intravenous injection of Ethyl cysteinate dimmer 555 MBq was performed before displaying cerebral images. Data of blood functional changing rat (BFCR%) were analyzed quantitatively using a mathematic model. Results: Before EA stimulation, the blood perfusion and function of cerebral cortex, thalamus, basal ganglion and cerebellum on both sides of the brain were basically symmetry. Following EA of MS 6, BFCR% of the contralateral thalamus, parietal lobe and the frontal lobe increased significantly. Following local infiltration anesthesia, BFCR% of the contralatral thalamus declined markedly (P<0.05). The results of quantitative analysis were in agreement with those of visual observation. Conclusion: Local infiltration anesthesia of the scalp point can significantly weaken or block EA stimulation induced changes of BFCR%.

EFFECTS OF COMPOUND ACUPUNCTURE AND MEDICINE ANESTHESIA ON TNF AND INF-Γ CONTENTS IN THE PATIENT OF PNEUMONECTOMY

Objective To explore effects of acupuncture on senJm tumor necrosis factor （TNF） and ,γ-inter- feron （INF-γ） contents in the operative patient of lung cancer. Methods In the first stage, electroacupuncture （EA） stimulation was given to 25 cases of lung cancer before operation, once daily for 3 consecutive days, with continuous EA stimulation during operation; EA was given once each day from the 5^th-7^th days after operation. In the second stage, EA was added from the 8^th- 10^th days after operation in 30 cases, once daily. Serum TNF and INF-γ contents were compared before and after operation. Results Serum TNF content on the 8th day after operation in the first stage study was lower （P〈0.05） and on the 12th day after operation in the second stage study was higher （P〈0.05） in the compound anesthesia group than that in the general anesthesia group; serum INF-γ contents in the compound anesthesia group on the 8^th day after operation in the first stage and on the 12^th day after operation in the second stage were significantly higher than those in the general anesthesia group （P〈0.01, P〈0.05）. Conclusion Acupuncture can regulate serum TNF and INF-γ contents in the patient of operation of lung cancer, with dual-directional regulation on immunologic function.

EFFECT OF ACUPUNCTURE COMBINED WITH EPIDURAL ANESTHESIA ON PLASMA CATECHOLAMINE CONTENT IN CHOLECYSTECTOMY PATIENTS

Objective: To observe changes of plasma catecholamine (CA) level in patients experiencing cholecystectomy under acupuncture anesthesia combined with epidural administration of small dose of anesthetics. Methods: 33 cholecystectomy patients were randomly divided into acupuncture combined with epidural anesthesia (A) group (n=11), acupoint skin electrical stimulation combined with epidural anesthesia (B) group (n=11) and simple epidural anesthesia (C) group (n=11). Acupoints used were bilateral Zusanli (ST 36) and Neiguan (PC 6) and stimulated with parameters of frequency 2/15 Hz, intermittent waves, electric current 2～3 mA for group A and 13mA for group B. Extradural anesthetic administered was 1.5% Lidocaine 5 mL. Venous blood samples were collected one day before, during and 3 days after operation for detecting plasma NE, E and DA contents. Results: ① During operation, plasma NE of group A and B lowered in comparison with pre operation, particularly group A (P<0.01), while in group C, plasma NE level increased slightly; plasma E of group A and B increased significantly compared with pre operation (P< 0.01). Plasma DA in the 3 groups all raised during operation. ② Three days after operation, plasma NE, E and DA levels recovered basically in comparison with those of one day before operation. It indicates that acupuncture or acupoint skin electrical stimulation is capable of regulating sympathetic activity during epidural anesthesia. The anesthetic effect has a closer relation with changes of plasma NE level rather than changes of plasma E or DA levels. Conclusion: Acupuncture or acupoint surface electrical stimulation combined with epidural anesthesia may be of reducing or releasing surgical operation generated stress response during cholecystectomy.

Protecting action of acupuncture-drug compound anesthesia with different frequency electroacupuncture on stress reaction in patients of pneumonectomy

Objective To observe the protective effect of acupuncture-drug compound anesthesia with different frequency electroacupuncture（EA） on stress reaction in patients of pneumonectomy and to explore potential mechanisms.Methods Eighty patients scheduled for pneumonectomy were randomly divided into four groups,group A,B,C and D,20 cases in each group.General anesthesia and single lung protective mechanical ventilation were carried out in all the groups.Acupuncture was given at Hòuxī（后溪 SI 3）,Zhīgōu（支沟TE 6）,Nèiguān（内关 PC 6）,and Hégǔ（合谷 LI 4） 30 min before general anesthesia,and the acupuncture needles were connected with Han＇s acupoint nerve stimulator（HANS-200） in all the groups,but the acupuncture needles without needle bodies were pasted on the acupoints and EA was not given in the group A.2 Hz continuous wave,100 Hz continuous wave and 2 Hz/100 Hz EA were given in the group B,C and D respectively.The supplementary amount of anesthesia medicine,heart rate（HR） and mean arterial pressure（MAP） during pneumonectomy,and CD4^＋/CD8^＋ in venous blood before and one day after the surgery,and the contents of epinephrine（E） and cortisol（Cor） in plasma after entering the operating room and before turning out the operating room were detected.Results ① During the operation,supplementary amounts of Fentanyl in the group B and C were lower than those in the group A and D（P〈0.05,P〈0.01）.② The MAP in the four groups at tracheal intubation（T1） all were higher than those before anesthesia（T0）（all P〈0.01）,and the ascending extents in the group B,C and D were lower than that in the group A（all P〈0.01）;HR at T1 in the group A was higher than that at T0（P〈0.05）,while no significant change in the other groups（all P〈0.05）,and the ascending extents in the group B and D were lower than that in the group A（both P〈0.05）;MAP and HR at the other moments in all the groups were stable.③ CD4^＋/CD8^＋ in the group A after pneumonectomy was lower than that before the surgery（P〈0.05）,while no significant change in the other groups（all P〈0.05）.④ Contents of E and Cor after the operation were significantly increased in all the groups（all P〈0.01）,and the ascending extent of E content in the group D was lower than that in the group A（P〈0.05）;the ascending level of Cor in the group B and D was lower than that in the group A（P〈0.01）,and in the group B was lower that in the group C and D.Conclusion Acupuncture-drug compound anesthesia can attenuate the stress reaction so as to protect organs under the condition of less or same narcotic amount and can alleviate the fluctuation of MAP and HR at tracheal intubation and stabilize CD4^＋/CD8^＋ after pneumonectomy.Among them,2 Hz and 2 Hz/100 Hz EA have better effects.

Effectiveness of acupuncture versus spinal-epidural anesthesia on labor pain： a randomized controlled trial

OBJECTIVE: To evaluate the effectiveness of acupuncture analgesia(AA) compared with combined spinal-epidural anesthesia(CSEA) for labor pain relief and labor outcomes.METHODS: We evaluated 131 primiparous women who received respiratory guidance during maternal uterine contractions and received either AA(n =43), CSEA(n = 45), or no additional treatment(control, n = 43). The groups were compared regarding visual analog scale(VAS) scores for abdominal and back pain, and labor outcomes.RESULTS: The abdominal VAS scores of the AA and CSEA groups were significantly lower than that of the control group. In addition, the VAS scores of the CSEA group were significantly lower than that of the AA group at 10 and 60 min after intervention.The back pain VAS scores of the AA and CSEA groups were significantly lower than that of the control group at 5, 10, and 60 min after intervention. The duration of the active phase of labor in the CSEA group was significantly longer than that of the AA and control groups. The rates of oxytocin use(4.70%), urinary retention(4.70%), and postpartum hemorrhage [(273.7 ± 53.6) m L] in the AA group were significantly lower than in the CSEA group [46.70%, 24.20%, and(320.0 ± 85.6) m L, respectively].CONCLUSION: Both AA and CSEA were effective for labor pain relief, CSEA provided more effective pain relief, while AA was associated with a shorter duration of labor and fewer adverse effects.and each has its advantages and disadvantages.

Effects of electroacupuncture plus drug anesthesia on pain and stress response in patients after radical surgery for stomach cancer

Objective:To observe the effect of electroacupuncture plus drug anesthesia on pain and stress response in patients after radical surgery for stomach cancer.Methods:A total of 60 patients were randomized into a control group and an observation group by the random number table,with 30 cases in each group.The control group was given conventional drug anesthesia.The observation group was given additional electroacupuncture intervention.Before anesthesia and 2 h,12 h and 24 h after surgery,the visual analog scale(VAS)was scored,the heart rate,the mean arterial pressure,and the levels of serumβ-endorphin(β-EP)and adrenocorticotrophic hormone(ACTH)were measured.Results:Two hours,12 h and 24 h after surgery,the VAS scores of both groups were higher than those before anesthesia(all P<0.05),and the VAS scores in the observation group were lower than those in the control group at the same time points(all P<0.05).Two hours,12 h and 24 h after surgery,the heart rates and mean arterial pressures in the control group were significantly higher than those before anesthesia(all P<0.05),while there were no significant intra-group differences in the observation group(all P>0.05),and the indicators were lower than those in the control group at the same time points(all P<0.05).Two hours,12 h and 24 h after surgery,the serumβ-EP levels in the observation group were significantly higher than those before anesthesia(all P<0.05),and significantly higher than those in the control group at the same time points(all P<0.05).Two hours,12 h and 24 h after surgery,the serum ACTH levels in the control group were significantly higher than those before anesthesia(all P<0.05),and were significantly higher than those in the observation group at the same time points(all P<0.05).Conclusion:Electroacupuncture plus drug anesthesia can significantly relieve pain and stress response in patients after radical surgery for stomach cancer.

Acupuncture Mechanisms: Anesthesia, Analgesia and Protection on Organ Functions

Acupuncture, as a healing art in traditional Chinese medicine, has been widely used to treat various diseases. In the history of acupuncture anesthesia, in the past decades, mechanisms of acupuncture analgesia has been widely investigated, and in recent years, acupuncture protection on organ functions has attracted great interest. This review summarized the research progress on mechanisms of acupuncture for analgesia and its protection against organ function injury in anesthesia, and its perspective of analgesia, immunomodulation, neuroendocrine regulation and multiple organ protection. The current evidence supports that acupuncture analgesia and its organ protection in anesthesia is associated with the integration of neuroendocrine-immune networks in the level of neurotransmitters, cytokines, hormones, neuronal ensembles,lymphocytes, and endocrine cells. Although the mechanisms of acupuncture analgesia and its organ protection are still not completely understood, basic as well as clinic researches on the mechanisms and applications of acupuncture and related techniques are being carried out.

Electroacupuncture compound anesthesia in radiofrequency ablation for hypertrophic inferior turbinate:a randomized controlled trial

Objective To explore the feasibility of electroacupuncture compound anesthesia in radiofrequency ablation for hypertrophic inferior turbinate.Methods The patients confirmed to the enrolled criteria were randomly divided into an observation group（n=31） and a control group（n=30）.In the observation group,electroacupuncture was applied to Sìbái（四白 ST 2）,Xiàguān（下关 ST 7）,Hégǔ（合谷 LI 4） and Zhīgōu（支沟 TE 6） on the left side for the anesthesia and the routine local anesthesia was done on the right side.In the control group,the routine local anesthesia was adopted on both sides.The feelings of pain,circulatory index and operation effect were observed and compared between the two groups.Results During radiofrequency ablation,the pain grades of two measurements on the left side and the 2nd measurement on the right in the observation group were all lower than those in the control group（all P〈0.05）.In the observation group,the pain grade on the left side was lower than that on the right side（P〈0.05）,and the systolic blood pressure and the heart rate were lower than those in the control group when undergoing the 2nd radiofrequency ablation on the right side and on the left side,respectively（all P〈0.05）.There was no significant difference in operation effect between the two groups.Conclusion Electroacupuncture compound anesthesia can meet the analgesia requirement of radiofrequency ablation for hypertrophic inferior turbinate,and would be helpful to prevent cyclic fluctuation during the operation at the same time.

Study on Acupuncture for Side Effects of Etomidate Anesthesia

Objective: To evaluate the effect of acupuncture therapy on the side effect of Etomidate anesthesia and to determine the best timing of acupuncture intervention during Etomidate anesthesia. Methods: Female patients with rectal cancer scheduled to receive radical resection were randomly divided into four groups: a control group(group Ⅰ), a preoperative acupuncture group(group Ⅱ), an intraoperative acupuncture group(group Ⅲ), and a postoperative acupuncture group(group Ⅳ), 25 subjects in each group. The levels of serum cortisol, blood glucose, and gastrin of patients were monitored at the following time-points: preoperative(T0, when having entered the operating room), intraoperative(T1, 30 min after skin cutting), the end of operation(T2, after suturing skin), and postoperative(T3, postoperative 24 h). The cases with massive hemorrhage, unsteady vital sign during operation or operation time longer than 5 h were eliminated, and 92 cases were enrolled into the results of the study. Results: The blood sugar in the four groups increased significantly at T2 and T3, compared to T0(P < 0.05); the blood sugar reached((.(((1.5() mmol(( at T2 in group Ⅲ, and the increase was statistically significant compared to those of the other groups(P<0.05). The levels of serum cortisol of all patients were in normal range. Compare to T0, the cortisol level decreased in group Ⅰ, Ⅲ, at each point, and the decreases Ⅳ were statistically significant(P<0.05). Compared to the other groups, the cortisol level was increased at T1 and T3 in group Ⅱ, and the change was statistically significant(P<0.05). Compared to T0, the gastrin level at T3 increased in group Ⅰ, Ⅲand Ⅳ, but decreased in group Ⅱ, and the changes had statistical significance(P<0.05). Compared to the other groups, the decrease of gastrin level in group Ⅱat T3 was statistically significant(P<0.05). Conclusion: Acupuncture before operation can keep blood sugar and serum cortisol steady during Etomidate anesthesia and reduce gastrin after Etomidate anesthesia, hence regulating the Etomidate-related stress responses effectively.