The safety of oxyclozanidc suspension was preliminarily evaluated through acute toxicity test in mice. Administration dose, formal trial grouping and group interval were determined in pre-trial using incremental metho...The safety of oxyclozanidc suspension was preliminarily evaluated through acute toxicity test in mice. Administration dose, formal trial grouping and group interval were determined in pre-trial using incremental method. Formal test was performed using simplified karber＇s method. Changes in sign of mice after ad- ministration were observed; the mortality rate was statistically calculated, and the time of death was recorded; the median lethal dose （LD50） and 95% confidence limit of oxyclozanide suspension were calculated. The results showed the LD50 of oxyclozanide suspension in mice by oral administration was 1. 679 g/kg, and the 95% confidence interval was 1. 439 - 1. 947 g/kg. According to toxicity grading of chemicals, oxyclozanide suspension was low toxic substance.展开更多
To observe the acute toxicity of recombinant porcine interferen-alpha （IFN-alpha） in mice and thus provide a basis for the clinical safety. [Method] According to the principles of acute toxicity, all the mice were d...To observe the acute toxicity of recombinant porcine interferen-alpha （IFN-alpha） in mice and thus provide a basis for the clinical safety. [Method] According to the principles of acute toxicity, all the mice were divided into two major groups （intraperitoneally injected group and intramuscularly injected group） respectively at high dose, moderate dose and low dose. And the normal control group was also set up. Within 14 d after administration, the behavior of mouse and the degree of toxicity were continuously observed. The hematological indexes and biochemical indexes of blood were detected to obtain the preliminary toxicity data of the recombinant porcine IFN-alpha. And at the end of the experiment, mice were sacrificed for autopsy. [ Result] There was not significant difference in external performance, behavioral characteristics, body temperature, weight, pathological anatomy of visceral organs, hematological indexes and biochemical indexes between the experimental groups and the control group. [ Conclusion] The highest dose of porcine interferon （5.0 x 10s IU per mouse） in this experiment or the dose lower than this dosage should not have significant toxic effects on mice, and the recombinant porcine IFN-alpha is safe in clinical application.展开更多
[Objective] The toxicity of Qiangli Bupi Paste was investigated, to provide a test basis for its further development and safe use. [Method] Forty Kunming mice, half male and half female, were selected and divided into...[Objective] The toxicity of Qiangli Bupi Paste was investigated, to provide a test basis for its further development and safe use. [Method] Forty Kunming mice, half male and half female, were selected and divided into the CK group and the Qiangli Bupi Paste group, each of which included 20 mice. The acute toxicity of Qiangli Bupi Paste was observed by the maximum administration dosage method. No mice and abnormal response were observed within 14 d in various groups. The tested animals were also subjected to anatomical observation. [Result] All the tested animals survived in the test, and behaved normally, with glossy hair. Their body weights accorded with the growth regularity. During the anatomy, no important vis- ceral organs showed pathological changes, and there were no significant differences in body weight between the two groups before administration, on the 7~ after ad- ministration and the 14 d after administration （P〉0.05）. [Conclusion] In the acute toxicity test of Qiangli Bupi Paste, the maximum administration dosage was 48 g/kg （equivalent to 96 times of the daily dose of adult in clinic）, and no obvious toxic response was observed.展开更多
The use of medicinal plants in South Africa is cultural. Withania somnifera is one of the medicinal plants used to treat various ailments in the country. The plant species has been used by traditional medicine practit...The use of medicinal plants in South Africa is cultural. Withania somnifera is one of the medicinal plants used to treat various ailments in the country. The plant species has been used by traditional medicine practitioners to treat inflammation and painful conditions like rheumatism. It is also known to be used as a sedative and hypnotic drug. Despite the claims, there is no information in literature to corroborate the therapeutic success of Withania somnifera in the treatment of inflammation and insomnia. The study, therefore, investigated the anti-inflammatory and central nervous system depressant activities of the leaf aqueous extract of the plant species in mice and rats. Fresh leaves of W. somnifera were collected from Kirstenbosch Botanical Gardens, South Africa, authenticated by a taxonomist and a voucher specimen （UWC 005） deposited in the University＇s Herbarium. Leaf aqueous extract was prepared using standard extraction methods. The carrageenan-induced rat paw oedema test was used to determine the anti-inflammatory effects while pentobarbitone-induced sleep and locomotor activity tests were used to evaluate the sedative effect of the plant species. Phytochemical qualitative analysis, acute toxicity and HPLC studies of the plant species were also carried out using standard methods. The phytochemical qualitative analysis carried out on the dried powdered leaves of W. somnifera showed the presence of saponins, tannins and triterpene steroids. Leaf aqueous extract of IV. somnifera （100-200 mg/kg IP） significantly prolonged pentobarbitone （40 mg/kg, i.p.）-induced sleep in mice in a dose dependant manner. Diazepam （0.5 mg/kg, i.p.） significantly prolonged pentobarbitone （40 mg/kg, i.p.）-induced sleep in mice. The doses of 100 and 200 mg/kg （i.p.） of the plant species and 0.5 mg/kg （i.p.） of diazepam significantly reduced the locomotor activity of mice. Leaf aqueous extract of the plant species （50-200 mg/kg, i.p.） significantly reduced the oedema produced by carrageenan （1%） in rats over 90 min period of testing. Indomethacin （20 mg/kg, i.p.） significantly reduced carrageenan （1%）-induced oedema in rats over 120 min period of testing. The LDs0 value obtained for the leaf aqueous extract of the plant species following inter-peritoneal injection was 1,600 mg/kg while that following oral administration was probably over 4,000 mg/kg. The HPLC finger-print of the aqueous extract showed distinct peaks at the following retention times 2.977, 3.594, 4.154, 4.406, 4.660 and 15.267 min. The results obtained show that leaf aqueous extract of W. somnifera has both sedative and anti-inflammatory effects.展开更多
基金Supported by National Key Technology R&D Program(2015BAD1101)Special Project of China Agricultural Industry Research System(CAR-38)
文摘The safety of oxyclozanidc suspension was preliminarily evaluated through acute toxicity test in mice. Administration dose, formal trial grouping and group interval were determined in pre-trial using incremental method. Formal test was performed using simplified karber＇s method. Changes in sign of mice after ad- ministration were observed; the mortality rate was statistically calculated, and the time of death was recorded; the median lethal dose （LD50） and 95% confidence limit of oxyclozanide suspension were calculated. The results showed the LD50 of oxyclozanide suspension in mice by oral administration was 1. 679 g/kg, and the 95% confidence interval was 1. 439 - 1. 947 g/kg. According to toxicity grading of chemicals, oxyclozanide suspension was low toxic substance.
基金Supported by Major Program of Natural Science Foundation of AnhuiProvince ( KJ2008A085)Anhui Key Technology R&D Program( 08010302179)~~
文摘To observe the acute toxicity of recombinant porcine interferen-alpha （IFN-alpha） in mice and thus provide a basis for the clinical safety. [Method] According to the principles of acute toxicity, all the mice were divided into two major groups （intraperitoneally injected group and intramuscularly injected group） respectively at high dose, moderate dose and low dose. And the normal control group was also set up. Within 14 d after administration, the behavior of mouse and the degree of toxicity were continuously observed. The hematological indexes and biochemical indexes of blood were detected to obtain the preliminary toxicity data of the recombinant porcine IFN-alpha. And at the end of the experiment, mice were sacrificed for autopsy. [ Result] There was not significant difference in external performance, behavioral characteristics, body temperature, weight, pathological anatomy of visceral organs, hematological indexes and biochemical indexes between the experimental groups and the control group. [ Conclusion] The highest dose of porcine interferon （5.0 x 10s IU per mouse） in this experiment or the dose lower than this dosage should not have significant toxic effects on mice, and the recombinant porcine IFN-alpha is safe in clinical application.
文摘[Objective] The toxicity of Qiangli Bupi Paste was investigated, to provide a test basis for its further development and safe use. [Method] Forty Kunming mice, half male and half female, were selected and divided into the CK group and the Qiangli Bupi Paste group, each of which included 20 mice. The acute toxicity of Qiangli Bupi Paste was observed by the maximum administration dosage method. No mice and abnormal response were observed within 14 d in various groups. The tested animals were also subjected to anatomical observation. [Result] All the tested animals survived in the test, and behaved normally, with glossy hair. Their body weights accorded with the growth regularity. During the anatomy, no important vis- ceral organs showed pathological changes, and there were no significant differences in body weight between the two groups before administration, on the 7~ after ad- ministration and the 14 d after administration （P〉0.05）. [Conclusion] In the acute toxicity test of Qiangli Bupi Paste, the maximum administration dosage was 48 g/kg （equivalent to 96 times of the daily dose of adult in clinic）, and no obvious toxic response was observed.
文摘The use of medicinal plants in South Africa is cultural. Withania somnifera is one of the medicinal plants used to treat various ailments in the country. The plant species has been used by traditional medicine practitioners to treat inflammation and painful conditions like rheumatism. It is also known to be used as a sedative and hypnotic drug. Despite the claims, there is no information in literature to corroborate the therapeutic success of Withania somnifera in the treatment of inflammation and insomnia. The study, therefore, investigated the anti-inflammatory and central nervous system depressant activities of the leaf aqueous extract of the plant species in mice and rats. Fresh leaves of W. somnifera were collected from Kirstenbosch Botanical Gardens, South Africa, authenticated by a taxonomist and a voucher specimen （UWC 005） deposited in the University＇s Herbarium. Leaf aqueous extract was prepared using standard extraction methods. The carrageenan-induced rat paw oedema test was used to determine the anti-inflammatory effects while pentobarbitone-induced sleep and locomotor activity tests were used to evaluate the sedative effect of the plant species. Phytochemical qualitative analysis, acute toxicity and HPLC studies of the plant species were also carried out using standard methods. The phytochemical qualitative analysis carried out on the dried powdered leaves of W. somnifera showed the presence of saponins, tannins and triterpene steroids. Leaf aqueous extract of IV. somnifera （100-200 mg/kg IP） significantly prolonged pentobarbitone （40 mg/kg, i.p.）-induced sleep in mice in a dose dependant manner. Diazepam （0.5 mg/kg, i.p.） significantly prolonged pentobarbitone （40 mg/kg, i.p.）-induced sleep in mice. The doses of 100 and 200 mg/kg （i.p.） of the plant species and 0.5 mg/kg （i.p.） of diazepam significantly reduced the locomotor activity of mice. Leaf aqueous extract of the plant species （50-200 mg/kg, i.p.） significantly reduced the oedema produced by carrageenan （1%） in rats over 90 min period of testing. Indomethacin （20 mg/kg, i.p.） significantly reduced carrageenan （1%）-induced oedema in rats over 120 min period of testing. The LDs0 value obtained for the leaf aqueous extract of the plant species following inter-peritoneal injection was 1,600 mg/kg while that following oral administration was probably over 4,000 mg/kg. The HPLC finger-print of the aqueous extract showed distinct peaks at the following retention times 2.977, 3.594, 4.154, 4.406, 4.660 and 15.267 min. The results obtained show that leaf aqueous extract of W. somnifera has both sedative and anti-inflammatory effects.
