The levels of thyroxine (T3, T4) and thyroid stimulating hormone (TSH) in rat serum were measured by radioimmunoassay, and also ultrastructure of the thyroid gland and adenohypophyseal cell were examined by transmissi...The levels of thyroxine (T3, T4) and thyroid stimulating hormone (TSH) in rat serum were measured by radioimmunoassay, and also ultrastructure of the thyroid gland and adenohypophyseal cell were examined by transmission electronic microscopy (TEM) after low concentration (0. 05 mg/kg) of samarium chloride (SmCl3) administration by intraperitoneal, oral and tail intravenous routes. The results showed that the level of T4 in rat serum increased and the level of TSH unchanged by the intraperitoneal administration, and the levels of T3, T4 and TSH did not increase by the oral and tail intravenous administration. The evidences mentioned above were in accord with the morphological changes of thyroid follicular epithelial cells andadenohypophysis thyrotroph observed under electronic microscope in a sense.展开更多
In cases of death caused by hypothermia,histological analysis can be used to determine the cause of death.Certain histological alterations of the pituitary glands in hypothermia have been reported in the literature,in...In cases of death caused by hypothermia,histological analysis can be used to determine the cause of death.Certain histological alterations of the pituitary glands in hypothermia have been reported in the literature,including haemorrhage,hyperaemia and cellular vacuolization of cells in the anterior lobe.In the present study,the validity of these morphological alterations as markers for fatal accidental hypothermia was investigated in autopsy material.A total of 34 pituitary glands in cases of verified fatal accidental hypothermia were examined histologically(haematoxylin and eosin,ferric,azan)and immunohistochemically(LCA,ACTH,C5b-9).The findings were compared with 61 cases in a control group.Hyperaemia was found in 50.0%of the study group cases and 59.0% of the control group cases.Cellular vacuolization was observed in one case(2.9%)in the study group and one case(1.6%)in the control group.Acute or recent haemorrhage in the glandular tissue was never detected.In our study,the histopathological characteristics described in the literature as pathognomonic for hypothermia could not be confirmed.Furthermore,histological differences in the pituitary glands between fatal hypothermia cases and control group cases were not observed.展开更多
文摘The levels of thyroxine (T3, T4) and thyroid stimulating hormone (TSH) in rat serum were measured by radioimmunoassay, and also ultrastructure of the thyroid gland and adenohypophyseal cell were examined by transmission electronic microscopy (TEM) after low concentration (0. 05 mg/kg) of samarium chloride (SmCl3) administration by intraperitoneal, oral and tail intravenous routes. The results showed that the level of T4 in rat serum increased and the level of TSH unchanged by the intraperitoneal administration, and the levels of T3, T4 and TSH did not increase by the oral and tail intravenous administration. The evidences mentioned above were in accord with the morphological changes of thyroid follicular epithelial cells andadenohypophysis thyrotroph observed under electronic microscope in a sense.
文摘In cases of death caused by hypothermia,histological analysis can be used to determine the cause of death.Certain histological alterations of the pituitary glands in hypothermia have been reported in the literature,including haemorrhage,hyperaemia and cellular vacuolization of cells in the anterior lobe.In the present study,the validity of these morphological alterations as markers for fatal accidental hypothermia was investigated in autopsy material.A total of 34 pituitary glands in cases of verified fatal accidental hypothermia were examined histologically(haematoxylin and eosin,ferric,azan)and immunohistochemically(LCA,ACTH,C5b-9).The findings were compared with 61 cases in a control group.Hyperaemia was found in 50.0%of the study group cases and 59.0% of the control group cases.Cellular vacuolization was observed in one case(2.9%)in the study group and one case(1.6%)in the control group.Acute or recent haemorrhage in the glandular tissue was never detected.In our study,the histopathological characteristics described in the literature as pathognomonic for hypothermia could not be confirmed.Furthermore,histological differences in the pituitary glands between fatal hypothermia cases and control group cases were not observed.
