The stream function and the velocity potential can be easily computed by solving the Poisson equations in a unique way for the global domain. Because of the var- ious assumptions for handling the boundary conditions, ...The stream function and the velocity potential can be easily computed by solving the Poisson equations in a unique way for the global domain. Because of the var- ious assumptions for handling the boundary conditions, the solution is not unique when a limited domain is concerned. Therefore, it is very important to reduce or eliminate the effects caused by the uncertain boundary condition. In this paper, an iterative and ad- justing method based on the Endlich iteration method is presented to compute the stream function and the velocity potential in limited domains. This method does not need an explicitly specifying boundary condition when used to obtain the effective solution, and it is proved to be successful in decomposing and reconstructing the horizontal wind field with very small errors. The convergence of the method depends on the relative value for the distances of grids in two different directions and the value of the adjusting factor. It is shown that applying the method in Arakawa grids and irregular domains can obtain the accurate vorticity and divergence and accurately decompose and reconstruct the original wind field. Hence, the iterative and adjusting method is accurate and reliable.展开更多
目的研究旨在探讨痤疮合剂对辅助性T细胞(Helper T Cell,Th)1、Th2、Th17、调节性T细胞(Regulatory T cell,Treg)平衡的调节作用。方法除对照组,其他干预组用Kligman造模法制造兔耳痤疮模型2周,取病理,判断造模成功后,痤疮合剂组灌服痤...目的研究旨在探讨痤疮合剂对辅助性T细胞(Helper T Cell,Th)1、Th2、Th17、调节性T细胞(Regulatory T cell,Treg)平衡的调节作用。方法除对照组,其他干预组用Kligman造模法制造兔耳痤疮模型2周,取病理,判断造模成功后,痤疮合剂组灌服痤疮合剂17.52 g/(kg·d);阳性药物组灌服清热暗疮胶囊0.12 g/(kg·d);对照组及模型组灌服等量生理盐水,1次/d,共30 d。取静脉血及患处组织、耳根淋巴结,苏木精-伊红染色法(Hematoxylin-eosin staining,HE)检测毛囊面积和皮脂腺直径。酶联免疫吸附测定法(Enzyme linked immunosorbent assay,ELISA)检测血清中干扰素(Interferon,IFN)-γ、白介素(Interleukin,IL)-5、IL-17、IL-12、IL-22、IL-10、转化生长因子(Transforming growth factor,TGF)-β含量。蛋白质印迹法(Western blot)、免疫组织化学法检测耳根淋巴结中T盒转录因子(T-box expressed in T cell,T-bet)、GATA结合蛋白3(GATA binding protein 3,GATA3)、视黄酸受体相关孤儿核受体γt(Retineic-acid-receptor-related orphan nuclear receptor gamma,RORγt)、叉头盒转录因子p3(Forkhead box P3,Foxp3)表达水平。结果痤疮合剂能明显改善兔耳表皮层的结节、脓疱、组织厚度、囊肿及毛囊口扩张程度,减少炎症细胞的增殖和浸润,表皮和内壁角质层变薄松软。与对照组相比,其余各组IFN-γ、IL-12、IL-17、IL-22含量显著增高,IL-5、TGF-β、IL-10含量显著降低(P<0.01)。与模型组比较,痤疮合剂和阳性组IFN-γ、IL-17、IL-12、IL-22含量明显下降,IL-5、TGF-β、IL-10含量显著增高(P<0.01)。与对照组比较,其余各组T-bet、RORγt的表达及平均光密度值(Integral-Optimistically,IOD)显著增大,GATA3、Foxp3表达及IOD下降(P<0.01)。与模型组相比,痤疮合剂和阳性组T-bet、RORγt表达及IOD下调,GATA3、Foxp3表达及IOD上调(P<0.01)。结论痤疮合剂通过调控炎性因子平衡Th1/Th2、Th17/Treg的极化偏移,实现免疫细胞内稳态,改善皮损病理表现,对痤疮有明显的治疗效果。展开更多
基金Project supported by the National Natural Science Foundation of China (No.40975031)
文摘The stream function and the velocity potential can be easily computed by solving the Poisson equations in a unique way for the global domain. Because of the var- ious assumptions for handling the boundary conditions, the solution is not unique when a limited domain is concerned. Therefore, it is very important to reduce or eliminate the effects caused by the uncertain boundary condition. In this paper, an iterative and ad- justing method based on the Endlich iteration method is presented to compute the stream function and the velocity potential in limited domains. This method does not need an explicitly specifying boundary condition when used to obtain the effective solution, and it is proved to be successful in decomposing and reconstructing the horizontal wind field with very small errors. The convergence of the method depends on the relative value for the distances of grids in two different directions and the value of the adjusting factor. It is shown that applying the method in Arakawa grids and irregular domains can obtain the accurate vorticity and divergence and accurately decompose and reconstruct the original wind field. Hence, the iterative and adjusting method is accurate and reliable.
