Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary...Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary to establish a model to screen biomarkers to characterize and evaluate aging degree. In this study,an in vitro aging model was set up by formaldehdye and human serum albumin( HSA),the most abundant protein in human plasma,based on Maillard Reaction. The liquid chromatography tandem mass spectrometry( LC-MS /MS) method with ^18O-labeling technique was employed to quantify modification degree of peptides cleaved from HSA. This model was established and optimized for further quantitative biomarker study.展开更多
基金Supported by the National Natural Science Foundation of China(21205005,81471919)MOST China(2011YQ0900502)1000 Plan
文摘Aging refers to a multidimensional process that all changes were accumulated in a person over time.These aging changes are associated with progressive increases in the chance of disease and death. Thus,it is necessary to establish a model to screen biomarkers to characterize and evaluate aging degree. In this study,an in vitro aging model was set up by formaldehdye and human serum albumin( HSA),the most abundant protein in human plasma,based on Maillard Reaction. The liquid chromatography tandem mass spectrometry( LC-MS /MS) method with ^18O-labeling technique was employed to quantify modification degree of peptides cleaved from HSA. This model was established and optimized for further quantitative biomarker study.