Bowel preparation protocol for hospitalized patients ages 50 years or older:A randomized controlled trial

BACKGROUND The incidence and mortality rate of colorectal cancer progressively increase with age and become particularly prominent after the age of 50 years.Therefore,the population that is≥50 years in age requires long-term and regular colonoscopies.Uncomfortable bowel preparation is the main reason preventing patients from undergoing regular colonoscopies.The standard bowel preparation regimen of 4-L polyethylene glycol(PEG)is effective but poorly tolerated.AIM To investigate an effective and comfortable bowel preparation regimen for hospitalized patients≥50 years in age.METHODS Patients were randomly assigned to group 1(2-L PEG+30-mL lactulose+a lowresidue diet)or group 2(4-L PEG).Adequate bowel preparation was defined as a Boston bowel preparation scale(BBPS)score of≥6,with a score of≥2 for each segment.Non-inferiority was prespecified with a margin of 10%.Additionally,the degree of comfort was assessed based on the comfort questionnaire.RESULTS The proportion of patients with a BBPS score of≥6 in group 1 was not significantly different from that in group 2,as demonstrated by intention-to-treat(91.2%vs 91.0%,P=0.953)and per-protocol(91.8%vs 91.0%,P=0.802)analyses.Furthermore,in patients≥75 years in age,the proportion of BBPS scores of≥6 in group 1 was not significantly different from that in group 2(90.9%vs 97.0%,P=0.716).Group 1 had higher comfort scores(8.85±1.162 vs 7.59±1.735,P<0.001),longer sleep duration(6.86±1.204 h vs 5.80±1.730 h,P<0.001),and fewer awakenings(1.42±1.183 vs 2.04±1.835,P=0.026)than group 2.CONCLUSION For hospitalized patients≥50 years in age,the bowel preparation regimen comprising 2-L PEG+30-mL lactulose+a low-residue diet produced a cleanse that was as effective as the 4-L PEG regimen and even provided better comfort.

Effect of Epoxy Resin on the Properties of Recycled Asphalt

To promote the recycling of reclaimed asphalt pavement(RAP),epoxy resin was used to prepare the epoxy-recycled asphalt mixtures.The effect of epoxy resin on the properties of aged asphalt binder was investigated based on the tensile test,flexural creep test,and laser scanning confocal microscopy.The curing characteristics and the mechanical performance of recycled asphalt with different epoxy contents were explored.The results show that the low-temperature performance,ductility,and strength of the aged asphalt binder were significantly improved when the epoxy content reached 40%.The curing time of epoxy-recycled asphalt should be at least 4 d to ensure the formation of good internal spatial network structure.

Evaluation of Compatibilizers for Improving Compatibility between Waste Vegetable Oil and Aged Asphalt

Modifying agents 2,2-Bis(4-glycidyloxyphenyl)propane(2BPE)and dibutyl phthalate(DBP)were selected to enhance the compatibility.By using molecular simulation software(Materials Studio,MS),nine systems were constructed,including molecular models of aged asphalt and WVO monomers with 2BPE and/or DBP.The solubility parameters,Flory-Huggins parameters,and interaction energies of these systems were calculated to determine the impact of 2BPE and DBP on the compatibility of WVO and aged asphalt.Results showed that the addition of 2BPE and DBP reduced the difference in the solubility parameters between WVO and aged asphalt,thus improving the compatibility between WVO and aged asphalt.Additionally,using a combination of 2BPE and DBP in both aged asphalt and rejuvenator was found to be more effective than using either 2BPE or DBP alone.Finally,it was determined that evaluating the compatibility of WVO and aged asphalt using Van der Waals potential and non-bonding energy as evaluation indicators was more accurate than using electrostatic potential energy.

Microbiomics and metabolomics insights into the microbial regulation on the formation of flavor components in the traditional fermentation process of Chinese Hongqu aged vinegar

This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavor components.Microbiome analysis demonstrated that Lactobacillus,Acetobacter,Bacillus,Enterobacter,Lactococcus,Leuconostoc and Weissella were the predominant bacterial genera,while Aspergillus piperis,Aspergillus oryzae,Monascus purpureus,Candida athensensis,C.xylopsoci,Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal species.Correlation analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine,acetoin and acetic acid,Lactococcus showed positive correlation with the production of 2-nonanone,2-heptanone,ethyl caprylate,ethyl caprate,1-hexanol,1-octanol and 1-octen-3-ol,C.xylopsoci and C.rugosa were positively associated with the production of diethyl malonate,2,3-butanediyl diacetate,acetoin,benzaldehyde and tetramethylpyrazine.Correspondingly,non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged vinegar.Correlation analysis revealed that Lactobacillus was significantly associated with DL-lactate,indolelactic acid,D-(+)-3-phenyllactic acid,pimelic acid,pregabalin and 3-aminobutanoic acid.This study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.

Geriatric nursing competence of clinical nursing staff at different hospital levels in Chongqing,China:A cross-sectional study

Objectives:This study aimed to survey the geriatric nursing competencies of clinical nursing staff in Chongqing City,China,and provide suggestions to enhance these competencies.Methods:This study was conducted in 204 hospitals in Southwest China from December 24,2022 to January 7,2023.The“Geriatric Nursing Competence of Clinical Nurse Investigation Tool”was used to explore factors that influence geriatric nurses’competencies via stratified sampling.The survey was conducted by distributing and collecting questionnaires through the online platform Wenjuanxing.Results:A total of 10,692 nurses answered the questionnaires.Of these questionnaires,9,442 were valid.The total geriatric nursing competence score of the clinical nursing staff was 2.29±0.81,the secondary hospital score was 2.23±0.78,and the tertiary hospital’s overall mean score was 2.33±0.83.The factors that influenced secondary hospitals included the department of medicine,age of nurses and total length of career(P<0.05).The factors that influenced tertiary hospitals included the department of medicine,age of nurses,nurses’professional title,and geriatric practical advanced nurses’certification(P<0.05).Conclusions:Geriatric nursing competence among clinical nursing staff is imbalanced at a lower-middle level and is influenced by various factors.Thefindings highlight the need for further clinical training in geriatric nursing.The training of geriatric nurses should focus on necessary clinical skills and on preparing them to adequately manage comprehensive geriatric syndromes.

Characteristics of the HIV/AIDS Epidemic among People Aged≥50 Years in China during 2018–2021

Objective This study aimed to determine the current epidemiological status of PLWHA aged≥50 years in China from 2018 to 2021.It also aimed to recommend targeted interventions for the prevention and treatment of HIV/AIDS in elderly patients.Methods Data on newly reported cases of PLWHA,aged≥50 years in China from 2018 to 2021,were collected using the CRIMS.Trend tests and spatial analyses were also conducted.Results Between 2018 and 2021,237,724 HIV/AIDS cases were reported among patients aged≥50 years in China.The main transmission route was heterosexual transmission(91.24%).Commercial heterosexual transmission(CHC)was the primary mode of transmission among males,while non-marital non-CHC([NMNCHC];60.59%)was the prevalent route in women.The proportion of patients with CHC decreased over time(Z=67.716,P<0.01),while that of patients with NMNCHC increased(Z=153.05,P<0.01).The sex ratio varied among the different modes of infection,and it peaked at 17.65 for CHC.The spatial analysis indicated spatial clustering,and the high-high clustering areas were mainly distributed in the southwestern and central-southern provinces.Conclusion In China,PLWHA,aged≥50 years,were predominantly infected through heterosexual transmission.The primary modes of infection were CHC and NMNCHC.There were variations in the sex ratio among different age groups,infected through various sexual behaviors.HIV/AIDS cases exhibited spatial clustering.Based on these results,the expansion of HIV testing,treatment,and integrated behavioral interventions in high-risk populations is recommended to enhance disease detection in key regions.

Laboratory Evaluation of Performance Recovery of Aged SBS-modified Asphalt Using Regenerants with Different Base Oil Components

In this study,the regenerative effects of different regenerants on aged SBS-modified asphalt from different perspectives were investigated,including their conventional properties,viscoelastic behavior,creep-related properties,and micromorphology.Base oils composed of different proportions of aromatic and saturated hydrocarbons as well as the styrene-butadiene-styrene(SBS)restorer were used to prepare the regenerants.The results showed that the components of the base oil of the regenerant played a crucial role in determining the characteristics and performance of the recycled SBSmodified asphalt.Regenerants containing a high proportion of aromatics dissolved the hard segment in the SBS restorer,thereby delaying the effect of a reduction in the regenerants on the performance of the aged asphalts at a high temperature.Regenerants containing a high proportion of saturates dissolved the soft segment in the SBS restorer to enhance the lowtemperature performance of the recycled asphalts.In addition,the stress sensitivity of the recycled asphalts increased with the fraction of aromatics in the regenerant.As the aromatic content of the base oil components of the regenerants increased and their saturate content decreased,the state of dispersion of the SBS phase in the recycled SBS-modified asphalts improved.The optimal content of aromatics in the base oil of the regenerants should be set in the range of 33%to 47%to ensure the adequate performance of the recycled asphalts and a high efficiency of the SBS restorer.

Physical-Rheological Properties and Performances of Rejuvenated(Styrene-Butadiene-Styrene)Asphalt with Polymerized-MDI and Aromatic Oil

Traditional asphalt rejuvenators,like aromatic oil(AO),are known to be effective in improving the low-temperature properties and fatigue performances of aged SBS(styrene-butadiene-styrene)modified asphalt(SBSMA)binders and mixtures.However,these rejuvenators inevitably compromise their high-temperature properties and deformation resistances because they dilute asphalt binder but do not fix the damaged structures of aged SBS.In this study,a highly-active chemical called polymerized 4,4-diphenylmethane diisocyanate(PMDI)was used to assist the traditional AO asphalt rejuvenator.The physical and rheological characteristics of rejuvenated SBSMA binders and the moisture-induced damage and rut deformation performances of corresponding mixtures were comparatively evaluated.The results showed that the increasing proportion of AO compromises the hightemperature property and hardness of aged SBSMA binder,and an appropriate amount of PMDI works to compensate such losses;3%rejuvenator at mass ratio of AO:PMDI=70:30 can have a rejuvenated SBSMA binder with a high-temperature performance similar to that of fresh binder,approximately at 71.4°C;the use of AO can help reduce the viscosity of PMDI rejuvenated SBSMA binder for improving its workability;PMDI can help improve the resistance of AO rejuvenated SBSMA binder to deformation,especially at elevated temperatures,through its chemical reactions with aged SBS;moisture induction can enhance the resistance to damage of rejuvenated mixtures containing AO/PMDI or only PMDI;and the rejuvenator with a mass ratio of AO:PMDI=70:30 can lead the rejuvenated mixture to meet the application requirement,with a rut depth of only 2.973 mm,although more PMDI can result in a higher resistance of rejuvenated mixtures to high-temperature deformation.

Application of real-time shear wave elastography to Achilles tendon hardness evaluation in older adults

BACKGROUND Real-time shear wave elastography(SWE)is a non-invasive imaging technique used to measure tissue stiffness by generating and tracking shear waves in real time.This advanced ultrasound-based method provides quantitative information regarding tissue elasticity,offering valuable insights into the mechanical properties of biological tissues.However,the application of real-time SWE in the musculoskeletal system and sports medicine has not been extensively studied.AIM To explore the practical value of real-time SWE for assessing Achilles tendon hardness in older adults.METHODS A total of 60 participants were enrolled in the present study,and differences in the elastic moduli of the bilateral Achilles tendons were compared among the following categories:(1)Age:55-60,60-65,and 65-70-years-old;(2)Sex:Male and female;(3)Laterality:Left and right sides;(4)Tendon state:Relaxed and tense state;and(5)Tendon segment:Proximal,middle,and distal.RESULTS There were no significant differences in the elastic moduli of the bilateral Achilles tendons when comparing by age or sex(P>0.05).There were,however,significant differences when comparing by tendon side,state,or segment(P<0.05).CONCLUSION Real-time SWE plays a significant role compared to other examination methods in the evaluation of Achilles tendon hardness in older adults.

Weldability of steel used in an aged bridge

A series of experiments was performed to investigate the weldability of steel used in an aged bridge.A steel material used in an aged railway bridge constructed in 1912 was extracted for this investigation.The chemical compositions of this steel were suitable for welding.However,the aged steel contained much sulfur.Cruciform welded joints were fabricated with this aged steel.Welding defects or cracks were not observed in the joints.The Vickers hardness test on the welded part did not confirm extreme hardening or softening.After yielding by the static tensile test,the cruciform joints were fractured at the welded parts.One of the specimens was fractured in the middle of the thickness of the aged steel.The Sulfur contained in the aged steel might cause this type of fracture.The results show that there may be a risk of brittle fracture not only from the welded part but also from the base metal.The chemical compositions of aged steel must be analyzed when repair welding is applied to the steel.

Evaluation of the Integrated Management Program for Acute Malnutrition in the Douentza Health District, Mopti Region, Mali

Introduction: Malnutrition is a major public health problem in Mali, despite the efforts of the government, its technical and financial partners. The aim of this study was to evaluate the integrated management program for acute malnutrition (IMPAM). Methodology: This was a descriptive cross-sectional study that took place from January to December 2020 in the Douentza health district. The study included anyone with at least one malnourished child aged 6 to 59 months in their care who agreed to take part in the study, community health center staff who had given their consent, and the URENI manager at the Douentza reference health center. Data collected via questionnaires were entered into Excel and then analyzed using Epi-Info version 7 software. Results: A total of 138 acutely malnourished children aged 6 - 59 months, including 71 girls (51.45%), 138 accompanying mothers and 11 health workers, were included in our case study. Among the malnourished, 54.34% were in the 12 - 23 months age group and 69.57% had the severe form. 93% of the mothers interviewed were satisfied with the care provided, and all the health staff interviewed stated that community conflicts had an impact on IMPAM’s activities. Cure rates were 81% in Moderate Outpatient Nutritional Recovery and Education Unit (URENAM), 84% in Severe Ambulatory Nutritional Recovery and Education Unit (URENAS) and 92% in Recovery and Intensive Nutritional Education Unit (URENI). Drop-out rates were 19% in URENAM, 16% in URENAS and 0% in URENI. The death rate was 8% in URENI and 0% in URENAM and URENAS. Conclusion: This study confirms the high prevalence of malnutrition in the district (10.54%). It also reveals that factors such as inter-community conflict and insecurity have seriously affected the IMPAM program.

Feeding Practices, Nutritional and Hemoglobin Status of Children Aged 6 to 59 Months in the Republic of Guinea: A Case Study of the Prefecture of Fria

Malnutrition is a major public health concern in developing countries,contributing to 45%of deaths among children under five years old.In the Republic of Guinea,the prevalence of acute malnutrition is estimated at 6.7%,largely influenced by inadequate breastfeeding and complementary feeding practices.A descriptive cross-sectional study was conducted in the Prefecture of Fria to assess the impact of feeding practices on the growth and development of children aged 6 to 59 months.A sample of 450 children residing in the prefecture was included in the study.The findings revealed a prevalence of acute malnutrition of 8.9%,while 4.4%of the children were underweight,and 7.1%suffered from chronic malnutrition.

Contraception among Women Aged 35 and over at the University Hospital Center of Brazzaville

Objective: To study contraception among women aged 35 and over at the University Hospital of Brazzaville. Population and Methods: This was a retrospective descriptive study, conducted from April 1st 2017 to January 31st 2022 in the Obstetrics and Gynecology Department of the University Hospital of Brazzaville, including women aged 35 years and over who had received a contraceptive method. The study variables were sociodemographic, reproductive, clinical and contraceptive method related. Results: Two hundred and thirty customers were collected during the study period, i.e., 10.8% of the patients who received contraception. They were 38.4 ± 3 years old on average, had secondary education (46.1%), were of low socioeconomic status (54.3%), lived with a partner (98.7%) and came from an urban area (97.4%). They were multigravida (85.2%), and multiparous (63.9%) with an average of 4 living children. The indications were of two types: convenience (26.2%) and medical (73.8%). The most commonly used contraceptives were implants (72.2%) and injectable progestin (20.5%). Conclusion: The indication for contraception for women over 35 years of age at the University Hospital Center of Brazzaville is more medical, with the use of long-acting contraceptives.

Risk Factors for Geo-Helminthiasis in Children Aged 6 - 36 Months in a Rural Health District in Cameroon

Introduction and Objectives: Soil-Transmitted-Helminthiasis (STH) is a public health problem in Cameroon. The control strategies currently in place, particularly chemoprevention, has shortcomings linked to the target population, which are school-age children. The objective was to determine the prevalence and the risk factors associated with geo-helminthiasis in children aged 0 to 3 years in a rural health district. Method: From December 2020 to May 2021, a descriptive and analytical cross-sectional study of 376 children between 6 and 36 months was carried out in the Akonolinga health district. This was a cluster sampling in 4 health areas. Stool samples were collected and analysed using the mini-FLOTAC method. The results expressed as the number of eggs per gram of stool. A questionnaire on socio-demographic and lifestyle data was administered to the parents. The Chi-squared test was used to measure the association between geo-helminth infection and the data collected. A multivariate analysis using logistic regression was performed (p 0.05). Results: The prevalence of STH was 19.4% (Ascaris lumbricoides: 16% and Trichuris trichiura: 8%). Risk factors were: consumption of contaminated water (AOR = 1.93 [1.03 - 3.6];p = 0.040), early contact of the child with the ground (before age of 4 months) (AOR = 4.9 [2.1 - 11.37];p .001), habit of walking barefoot (AOR = 2.91 [1.1 - 7.97];p = 0.038), and living in a habitat with unpaved ground (AOR = 7.4 [1.55 - 35.7];p = 0.012). Conclusion: The prevalence of STHs in infants was high. Preventive chemotherapy should be extended to this age-group, and other measures intensified.

Factors Associated with Acute Respiratory Infections in Children Aged 0 - 5 Years in the Yénawa District of Cotonou (Benin) in 2023

Introduction: Acute respiratory infections remain one of the main causes of mortality in children aged 0 to 5. This work aimed to study the associated factors with the occurrence of acute respiratory infections in children 0 to 5 years old in Yénawa, Cotonou in 2023. Subjects and Method: It was an analytical cross-sectional study of children aged 0 - 5 years and their mothers in Yénawa, selected by four-degree random sampling. The sampling size, calculated using the Schwartz formula, was 126 children and 126 mothers. The dependent variable was the occurrence of acute respiratory infections. The independent variables were classified into four groups: socio-demographic and economic characteristics, behavioral factors, child-related factors, and environmental factors. Data collected by observation and questionnaire survey were analyzed using STATA version 15 software. Associated factors were investigated by bivariate analysis and multiple logistic regression, at the 5% significance level. Results: A total of 126 children aged 0 - 5 years and 126 mothers were surveyed, aged 23.5 (11 - 36) months and 30 (18 - 48) years respectively. The prevalence of acute respiratory infections was 74.60% (CI95% = 66.89 to 82.30). The associated factors were the mother’s age between 18 and 28 (OR = 10.77;CI95% = 1.89 to 61.27;p = 0.007), the use of charcoal/wood for cooking (OR = 7.36;IC = 1.99 to 27.10;p = 0.003)), children's poor personal hygiene (OR = 8.87;IC = 2.92 to 26.97;p 0.001)), and cohabitation with domestic animals (OR = 7.27;IC = 1.67 to 31.71;p = 0.015). Conclusion: Communicating with mothers about the factors identified will help reduce the prevalence of acute respiratory infections in children aged 0 to 5.

Epidemio-Clinical Profile and Factors Associated with Performance Indicators of Integrated Management of Severe Acute Malnutrition in Children Aged 6 - 59 Months Hospitalized at the Kalaban Coro Reference Health Center

Introduction: Malnutrition is an important reason for consultation in Mali’s health facilities and remains a major public health problem. The aim of this study was to describe the epidemioclinical profile and associated factors with performance indicators of integrated management of severe acute malnutrition in children aged 06 to 59 months. Methodology: this was a cross-sectional study with retrospective data collection (January 2021 to December 2022). All children hospitalized for severe acute malnutrition in the pediatric department and whose medical records were usable were included. Data collected using a standardized questionnaire was analyzed with SPSS Version 20 software. Results: A total of 534 children were included. The 12 to 23 months age group (49.1%) and the female sex (53.18%) were the most affected. Fully vaccinated children by age represented 49.4%. The predominant form of malnutrition was marasmus (77.7%). Diarrhea/vomiting (30.3%), fever (18.4%) and cough (15.5%) were the main reasons for consultations. Cure, discontinuation and death rates were 78.5%, 2.1% and 9.2%, respectively. On univariate analysis, the factors statistically associated with performance indicators (cure, drop-out, death) were gastroenteritis (P-value Conclusion: This study reveals that the frequency of severe acute malnutrition remains high at the Kalaban Coro reference health center. Better prevention of illnesses such as malaria, gastroenteritis, and respiratory infections, as well as timely referral, could help facilitate its management.

Factors Associated with Mortality in Children Aged 1 Month to 15 Years Hospitalized in the Pediatric Ward of the Kalaban-Coro Reference Health Center: Cross-Sectional Study

Introduction: Infant and child mortality is a worldwide concern, but developing countries such as Mali are more affected. The aim of this study was to investigate morbidity and factors associated with mortality in children aged 1 month to 15 years. Methodology: This was a cross-sectional study which took place from January 1 to December 31, 2020 covering children aged 1 month to 15 years hospitalized at the Kalaban-Coro CSRéf. Data were entered into Excel and analyzed using SPSS version 20 software. Results: Five hundred children aged 1 months to 15 years were included. The age range 1 to 5 years (53.6%) and male sex (58.2%) were the most represented. Malaria (72.2%), acute respiratory infections (6.2%) and diarrhea/dehydration (3%) were the main morbidities. Mortality was estimated at 10.6%, and the two main causes of death were malaria (56.6%) and acute respiratory infections (7.54%). Univariate analysis revealed a statistically significant association between the dependent variable (death) and age (p Conclusion: This study confirms the high rate of infant and child morbidity and mortality in our health facilities. Strengthening human resources and intensifying behavior-change communication can help reverse the trend.

Constructing Online Open Courses in Geriatric Nursing for Secondary Vocational Schools Using the OBE Framework

Based on the Outcome-Based Education(OBE)concept,the design of the secondary vocational online open course“Geriatric Nursing”plays a crucial role in enabling students to effectively acquire relevant knowledge and skills in geriatric nursing.First,an integrated design of learning outcomes should be established in line with the OBE framework,clearly outlining the competency levels students must achieve in geriatric nursing.Second,a structured curriculum must be developed to address the practical needs of geriatric nursing roles,ensuring course content aligns with real-world applications.Lastly,it is essential to implement a student-centered approach that combines online and offline teaching,prioritizing the learning experience and personalized needs of students.Adhering to the principles of“integrated design,structured curriculum,and blended teaching,”the development of the“Geriatric Nursing”online open course facilitates the transition from discipline-oriented to goal-oriented instruction and shifts the focus from teacher-centered to student-centered learning,ultimately advancing the training of geriatric nursing professionals.

Research Progress on the Relationship Between Dietary Inflammation Index and Geriatricrelated Diseases

Along with the acceleration of aging in our country and the increased prioritization on healthy living,the prevention and treatment of senile diseases have become a hot topic.The Dietary Inflammation Index(DII)is a new tool for assessing dietary inflammation.This article reviewed the research processes on the association between DII and age-related diseases such as osteoporosis,cognitive impairment,cardiovascular disease,cancer,and so on.It is expected that DII can provide guidance on the daily diet of older people,prevent and treat age-related diseases,and improve their quality of life.

EB病毒抗体检测在鼻咽癌早诊应用中的评价

作者在广东鼻咽癌高发区四会市、广州市的５４４２５名。年龄在３０－５９岁自然人群中，应用多项ＥＢ病毒（ＥＢＶ）血清学指标结合鼻咽光导纤维镜、病理组织学检查等手段，进行前瞻性观察、验证及评价这些鼻咽癌“高危人群”血清学指标的应用价值。我们认为：在鼻咽癌高发区人群筛查中，应用ＥＥＶ抗体检测，凡具下列指标之一者。为患鼻咽癌高危人群，应给予鼻咽光纤镜及病理检查，以期发现早期病人。①ＥＢＶＩｇＡ／ＶＣＡ≥１：８０或ＥＤＡｂ≥５０％。②ＥＢＶＩｇＡ／ＶＣＡ、ＩｇＡ／ＥＡ、ＥＤＡｂ３项中任何两项阳性。③ＥＢＶＩｇＡ／ＶＣＡ、ＩｇＡ／ＥＡ、ＥＤＡｂ３项中任何单项持续高滴度或滴度持续上升。