Based on data collected by 3 basic weather stations in Fushun City and 47 automatic weather stations in the whole region,we selected indexes suitable for agro-climatic division of Rana chensinensis,and carried out a s...Based on data collected by 3 basic weather stations in Fushun City and 47 automatic weather stations in the whole region,we selected indexes suitable for agro-climatic division of Rana chensinensis,and carried out a study on agro-climatic division of Rana chensinensis in this region.Annual active accumulated temperature ≥10 ℃ and precipitation during April and September are used as basic calculation factors,and we use climatic aridity to make climatic division of Rana chensinensis in Fushun City.According to relevant indexes,we divide Fushun City into 3 regions,namely most appropriate,appropriate and general regions.Finally,countermeasures and suggestions are put forward for providing reference for agricultural production.展开更多
[Objective] The aim was to analyze and explore division of agro-climatic zones of Beibei District in Chongqing municipality. [Method] According to major triple-cropping crops in Beibei District, such as wheat (spring...[Objective] The aim was to analyze and explore division of agro-climatic zones of Beibei District in Chongqing municipality. [Method] According to major triple-cropping crops in Beibei District, such as wheat (spring and winter), rice (midmaturation early season rice, later-maturation early rice, later-maturation late rice and middle-season rice), heat demanded by rapeseed, different cultivation methods and cultivation regions with different crop varieties, the accumulated temperatures in four cultivation regions featured by special cultivation methods and variety-matching are concluded. Based on average and accumulated temperatures in different locations in 30 y and other factors, such as terrain, elevation, rainfall and soil property, Beibei District can be classified into four agro-climatic zones. [Result] Four cultivation regions are as follows: the 1st cultivation region where accumulated temperature in T y is ≥6 530 ℃, the 2nd cultivation region where 6 530 ℃ accumulated temperature in T y≥6 350 ℃, the 3rd cultivation region where 6 530 ℃ accumulated temperature in T y≥5 990 ℃, and the 4th cultivation region where accumulated temperature in T y is ≤5 990 ℃. Beibei District can be classified into four agro-climatic zones, involving lower hill temperate zone, higher hill temperate zone, single-slope lower hills and karst temperate zone, and lower ridge cool zone. [Conclusion] Based on characters of four agro-climatic zones and accumulated temperatures required in four cultivation regions, agro-climatic zones can be classified in Beibei District, providing references for rational arrangement of crops and is of significance for stabilization of agricultural production, improvement of crops and increase of farmers’ income.展开更多
This paper provides a method of the process of computation called the cumulative method, it is based upon repeated cumulative process. The cumulative method is being adapted to the purposes of computation, particularl...This paper provides a method of the process of computation called the cumulative method, it is based upon repeated cumulative process. The cumulative method is being adapted to the purposes of computation, particularly multiplication and division. The operations of multiplication and division are represented by algebraic formulas. An advantage of the method is that the cumulative process can be performed on decimal numbers. The present paper aims to establish a basic and useful formula valid for the two fundamental arithmetic operations of multiplication and division. The new cumulative method proved to be more flexible and made it possible to extend the multiplication and division based on repeated addition/subtraction to decimal numbers.展开更多
Landslide susceptibility assessment is an essential tool for disaster prevention and management. In areas with multiple fault zones, the impact of fault zone on slope stability cannot be disregarded. This study perfor...Landslide susceptibility assessment is an essential tool for disaster prevention and management. In areas with multiple fault zones, the impact of fault zone on slope stability cannot be disregarded. This study performed qualitative analysis of fault zones and proposed a zoning method to assess the landslide susceptibility in Chengkou County, Chongqing Municipality, China. The region within a distance of 1 km from the faults was designated as sub-zone A, while the remaining area was labeled as sub-zone B. To accomplish the assessment, a dataset comprising 388 historical landslides and 388 non-landslide points was used to train the random forest model. 10-fold cross-validation was utilized to select the training and testing datasets for the model. The results of the models were analyzed and discussed, with a focus on model performance and prediction uncertainty. By implementing the proposed division strategy based on fault zone, the accuracy, precision, recall, F-score, and AUC of both two sub-zones surpassed those of the whole region. In comparison to the results obtained for the whole region, sub-zone B exhibited an increase in AUC by 6.15%, while sub-zone A demonstrated a corresponding increase of 1.66%. Moreover, the results of 100 random realizations indicated that the division strategy has little effect on the prediction uncertainty. This study introduces a novel approach to enhance the prediction accuracy of the landslide susceptibility mapping model in areas with multiple fault zones.展开更多
BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M phase.Recent research has shown that CDC25C could be a pot...BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M phase.Recent research has shown that CDC25C could be a potential therapeutic target for cancers,particularly for hepatocellular carcinoma(HCC).However,the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely understood.AIM To explore the impact of CDC25C on cell proliferation and apoptosis,as well as its regulatory mechanisms in HCC development.METHODS Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences(LV-CDC25C shRNA)to knock down CDC25C.Subsequently,a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in vivo.Cell proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays,respectively.The expression of endoplasmic reticulum(ER)stress-related molecules(glucose-regulated protein 78,X-box binding protein-1,and C/EBP homologous protein)was measured in both cells and subcutaneous xenografts using quantitative real-time PCR(qRT-PCR)and western blotting.Additionally,apoptosis was investigated using flow cytometry,qRT-PCR,and western blotting.RESULTS CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction.A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in mice.CDC25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response,ultimately promoting ER stress-induced apoptosis in HCC cells.CONCLUSION The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signaling pathway.展开更多
Rice production is crucial for food security in China,and its relationship with rural labor migration has been studied extensively.Labor migration in rural China has taken new forms in recent years.There has been a di...Rice production is crucial for food security in China,and its relationship with rural labor migration has been studied extensively.Labor migration in rural China has taken new forms in recent years.There has been a discernible trend wherein adult children have started migrating to cities while their elderly parents return to villages to re-engage in on-farm work.The phenomenon has notably shaped the intergenerational division of labor(IDL)within households.However,it remains to be seen how farmers adjust their rice production systems in response to the IDL.The age of 60 years for employment injury insurance is the eligibility threshold for off-farm employment and is used to obtain a source of exogenous variation in the IDL.Based on a representative household survey of 1,752 rice farmers in the Hubei Province of Central China,our fuzzy regression discontinuity analysis reveals that farmers in IDL households are more likely to adopt ratoon rice(RR)than single cropping rice(SR)or double cropping rice(DR).The effect of the IDL varies under different levels of operational scales and specialized agricultural service availability.Further analysis suggests that farmers’arrangements are associated with two potential mechanisms of downward intergenerational transfer.Monetary transfer for urban housing purchases increases RR in IDL households,and time transfer for intergenerational childcare significantly promotes SR in IDL households.This study enhances the understanding of the relationship between rural labor migration and rice production in China,providing a reference for adjusting rice production systems to ensure food security.展开更多
The Zambezi Delta basin is a passive marginal basin located on the East African coast that has good oil and gas exploration potential.Due to the special geological evolutionary background of the Beira High in the Zamb...The Zambezi Delta basin is a passive marginal basin located on the East African coast that has good oil and gas exploration potential.Due to the special geological evolutionary background of the Beira High in the Zambezi Delta basin,it has a low gravity anomaly,and the existing seismic survey lines do not cover the whole basin;therefore,it is difficult to interpret the structural characteristics of the whole basin based solely on gravity or seismic data.Based on satellite altimetry gravity anomaly data,this study infers the distribution characteristics of faults in the Zambezi Delta basin by using the normalized vertical derivative of the total horizontal derivative(NVDR-THDR)technique.Then,constrained by seismic data,the gravity anomaly at the Moho interface is extracted by using the fast forward method of the double-interface model of the gravity anomaly,and this anomaly is then removed from the Bouguer gravity anomaly to obtain the sedimentary layer gravity anomaly.The thickness of the sedimentary strata is obtained by inversing the sedimentary basement depth of the whole basin.Then,uplifts and depressions are divided based on a sedimentary layer thickness of 3 km.This research demonstrates that the Zambezi Delta basin mainly features nearly SN-trending and NE-trending faults and that these faults exhibit east-west partitioning.The nearly SN-trending strike-slip faults controlled the sedimentary development of the basin,and the NE-trending tensile faults may have acted as migration channels for oil,gas and magma.The“overcompensation”effect of the Moho interface gravity anomaly on the gravity anomaly of the sedimentary layer is caused by the depression of the Moho interface beneath the Beira High,which results in a low gravity anomaly value for the Beira High.The pattern of uplifts and depressions trends NE and has the structural characteristics of east-west blocks.展开更多
Administrative regions are an important environment for the operation of China's market economy.The relevant economic subjects cannot predict the policy of adjustment of administrative divisions and carry out cons...Administrative regions are an important environment for the operation of China's market economy.The relevant economic subjects cannot predict the policy of adjustment of administrative divisions and carry out conscious migration behavior,adjustment of administrative divisions can be regarded as a quasi-natural experiment.The three cities of Hefei,Wuhu,and Ma'anshan,which are directly related to the adjustment of the administrative division of Chaohu,are taken as the treatment group,and the seven adjacent cities of Lu'an,Huainan,Chuzhou,Bengbu,Anqing,Chizhou,and Tongling are taken as the control group.Differences-in-Differences method and relevant control variables affecting the upgrading of industrial structure are used to test.The test results show that"Partitions of Chaohu"has a significant industrial structure upgrading effect by promoting the optimization of spatial layout,the cross-regional flow of production factors and the effective management of Chaohu Lake Basin.At the same time,the increase of total retail sales of consumer goods,urban fixed assets investment,public utility expenses in science,education,culture and health,and population plays a significant positive role in promoting the upgrading of industrial structure,while foreign direct investment plays a certain inhibition role in the upgrading of industrial structure.In order to meet the ever-developing space demands and enhance the impact on surrounding areas,the Hefei metropolitan area should be driven by technological innovation,strengthen the integration of industrial chains,improve the business environment and transportation network,and continuously promote the upgrading of industrial structure and the formation and development of new productive forces.展开更多
In this work, we present a general theoretical study leading to analytical expression of the seasonal temperature at the near surface that is expected to evaluate any area seasonal temperature of the world using the l...In this work, we present a general theoretical study leading to analytical expression of the seasonal temperature at the near surface that is expected to evaluate any area seasonal temperature of the world using the least square method to fit the hourly data to the theoretical curve of the temperature. It is shown that the temperature is globally the result of two contributions: the contribution of the revolution movement of the terrestrial globe on its elliptical orbit around the sun, the contribution of the spin-orbit coupling for the rotation movement of the terrestrial globe around its polar axis and its revolution movement. The orbital behavior of the temperature is used to find the seasonal divisions of the climate for the local area considered. The whole expression of the temperature is very useful for the meteorological needs. The contribution of the human activities and natural instabilities are the results of discrepancies which increase errors (standard deviations).展开更多
As an important group of plant cellular organelles, the molecular mechanism of plastid division is poorly understood. Recent studies have revealed that the homologs of ftsZ gene, an essential prokaryotic cell division...As an important group of plant cellular organelles, the molecular mechanism of plastid division is poorly understood. Recent studies have revealed that the homologs of ftsZ gene, an essential prokaryotic cell division gene, are involved in plastid division process of plant cells. Antisense and sense expression constructions were employed to investigate the functions of the two ftsZ genes, NtFtsZ1 and NtFtsZ2, in transgenic Nicotiana tabacum L. plants. Although antisense expression of,NtFtsZs reduced the native protein level obviously, the size and number of chloroplasts in transgenic tobacco plants had no effect. In contrast, overexpression of NtFtsZs in transgenic plants strikingly changed the number and morphology of chloroplasts. Even only 1 - 2 huge chloroplasts could be seen in the mesophyll cells of some overexpression transgenic plants. Analyses of chloroplast ultrastructures and chlorophyll content of different transgenic plants suggested that NtFtsZs gene have no direct influence on the normal development and function of chloroplasts. ne changes in chloroplast morphology must be a compensation for the change in chloroplast number. The different phenotypes of chloroplasts in antisense and sense transgenic plants implied that different members from the same ftsZ gene family may have similar function in controlling plastid division. Meanwhile, the changes of chloroplast morphology in sense transgenic plants represented the possible plastoskeleton function of ftsZ in higher plant.展开更多
The average rainfall in whole growth period of winter wheat to plus or minus several times of sample variance was used to define different grades of drought.The reduction rate of production in meteorological which was...The average rainfall in whole growth period of winter wheat to plus or minus several times of sample variance was used to define different grades of drought.The reduction rate of production in meteorological which was equal to or greater than the rate of 3% was taken as standards to define wheat drought disaster years.The average reduction ratio of production in the wheat drought years in Hebei-Shandong-Henan was calculated.The frequency distribution rule of wheat in different drought disaster strength of winter wheat in Hebei-Shandong-Henan was analyzed.According to the conception and calculation method of drought disaster risk index of winter wheat,the regional distribution law of drought disaster risk index of winter wheat was analyzed.The division technical methods of winter wheat drought disaster risk zones in Hebei-Shandong-Henan under the irrigated conditions were put forward.Taking the average reduction rate of production in drought years,drought disaster risk index and precipitation of winter wheat in growth periods as indices,using the statistical analysis and overlay function of GIS,the production reduction risk of winter wheat caused by drought disaster in winter wheat zones of Hebei-Shandong-Henan was divided and evaluated.The risk evaluation of production reduction of winter wheat in different risk zones under different climate conditions was realized.The disaster prevention and mitigation measures of winter wheat drought were given.展开更多
Ground roll waves interfere with seismic data. The suppression of ground roll waves based on the division of wavelet frequencies considers the low-frequency characteristics of ground roll waves. However, this method w...Ground roll waves interfere with seismic data. The suppression of ground roll waves based on the division of wavelet frequencies considers the low-frequency characteristics of ground roll waves. However, this method will not be effective when the ground roll wave and the effective signal have the same frequency bands because of overlapping. The radial trace transform (RTT) considers the apparent velocity difference between the effective signal and the ground roll wave to suppress the latter, but affects the low-frequency components of the former. This study proposes a ground roll wave suppression method by combining the wavelet frequency division and the RTT based on the difference between the ground roll wave velocity and the effective signal and their energy difference in the wavelet domain, thus making full use of the advantages of both methods. First, we decompose the seismic data into different frequency bands through wavelet transform. Second, the RTT and low-cut filtering are applied to the low-frequency band, where the ground roll waves are appearing. Third, we reconstruct the seismic record without ground roll waves by using the inverse RTT and the remaining frequency bands. The proposed method not only improves the ground roll wave suppression, but also protects the signal integrity. The numerical simulation and real seismic data processing results suggest that the proposed method has a strong ability to denoise while preserving the amplitude.展开更多
Two plastid division genes, NtFtsZ1 and NtFtsZ2 isolated from Nicotiana tabacum L. were fused with gfp and expressed in Escherichia coli . The regular localizations of full length NtFtsZs∶GFP along the fil...Two plastid division genes, NtFtsZ1 and NtFtsZ2 isolated from Nicotiana tabacum L. were fused with gfp and expressed in Escherichia coli . The regular localizations of full length NtFtsZs∶GFP along the filamentous bacteria indicated that the NtFtsZs could recognize the potential division sites in E. coli and be polymerized with heterogeneous FtsZ from bacteria. The overexpression of NtFtsZs ∶ gfp inhibited the division of host strain cells and resulted in the long filamentous bacterial morphology. These results suggested that eukaryotic ftsZs have similar function to their prokaryotic homologs. Meanwhile, the different deletions of motifs of NtFtsZs are also employed to investigate the functions of these proteins in E. coli . The results showed that the C_terminal domains of NtFtsZs were related to the correct localization of NtFtsZs in E. coli and the N_terminal domains of NtFtsZs were responsible for the polymerization of homogeneous and heterogeneous FtsZ proteins. The significance of these results in understanding the functions of NtFtsZs in plastid division were discussed.展开更多
With the aim to improve the level of monitoring and warning as well as the comprehensive control of rice blast disease, and to feasibly reduce the disease threat in Nanchong City, the methods of GPS and GIS, systemati...With the aim to improve the level of monitoring and warning as well as the comprehensive control of rice blast disease, and to feasibly reduce the disease threat in Nanchong City, the methods of GPS and GIS, systematical monitoring and field survey, rice blast resistance identification, physiologic races of rice blast monitoring, and meteorological data analysis were performed to study the occurrence and epidemic region division, precise demarcation and occurrence and epidemic regularity of rice blast in Nanchong City. This study first completed the epidemic region division and precise demarcation; first clarified the initial affection(beginning period) locations, occurrence characteristics, epidemic trends and characteristics; explicated the existence of four epidemic peak periods of rice blast in the field, where the damage areas of first peak period played a decisive role during the blast epidemic years; in late May, the cumulative occurrence areas and annual occurrence areas presented higher positive correlation with the correlation coefficient of 0.817;and established a prediction model of occurrence areas per year based on the disease field rate at the end of boot stages and the diseased plant rate at dough stages. The results of investigation on the impact factors investigation of blast disease in Nanchong in recent years suggested that the internal causes were the decrease or loss of blast resistance of the rice cultivars, as well as the increase of physiological races with strong resistance to rice blast and the emergence of new virulent physiologic varieties; the external causes were suitable temperature, too much rainy, and sunlight shortage. Between 2010 and 2015, the short-term forecast accuracy for rice blast in Nanchong was up to 100%, and medium-and long-term forecast accuracy was also up to 98% and 95%, respectively, which increased by 5-15% than that before 1997, thereby making the control effect of rice blast in Nanchong increased by 15-30%.展开更多
Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was m...Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding.展开更多
文摘Based on data collected by 3 basic weather stations in Fushun City and 47 automatic weather stations in the whole region,we selected indexes suitable for agro-climatic division of Rana chensinensis,and carried out a study on agro-climatic division of Rana chensinensis in this region.Annual active accumulated temperature ≥10 ℃ and precipitation during April and September are used as basic calculation factors,and we use climatic aridity to make climatic division of Rana chensinensis in Fushun City.According to relevant indexes,we divide Fushun City into 3 regions,namely most appropriate,appropriate and general regions.Finally,countermeasures and suggestions are put forward for providing reference for agricultural production.
基金Supported by Key Technology Research and Development Program of Beibei District in Chongqing municipality (2012-11)~~
文摘[Objective] The aim was to analyze and explore division of agro-climatic zones of Beibei District in Chongqing municipality. [Method] According to major triple-cropping crops in Beibei District, such as wheat (spring and winter), rice (midmaturation early season rice, later-maturation early rice, later-maturation late rice and middle-season rice), heat demanded by rapeseed, different cultivation methods and cultivation regions with different crop varieties, the accumulated temperatures in four cultivation regions featured by special cultivation methods and variety-matching are concluded. Based on average and accumulated temperatures in different locations in 30 y and other factors, such as terrain, elevation, rainfall and soil property, Beibei District can be classified into four agro-climatic zones. [Result] Four cultivation regions are as follows: the 1st cultivation region where accumulated temperature in T y is ≥6 530 ℃, the 2nd cultivation region where 6 530 ℃ accumulated temperature in T y≥6 350 ℃, the 3rd cultivation region where 6 530 ℃ accumulated temperature in T y≥5 990 ℃, and the 4th cultivation region where accumulated temperature in T y is ≤5 990 ℃. Beibei District can be classified into four agro-climatic zones, involving lower hill temperate zone, higher hill temperate zone, single-slope lower hills and karst temperate zone, and lower ridge cool zone. [Conclusion] Based on characters of four agro-climatic zones and accumulated temperatures required in four cultivation regions, agro-climatic zones can be classified in Beibei District, providing references for rational arrangement of crops and is of significance for stabilization of agricultural production, improvement of crops and increase of farmers’ income.
文摘This paper provides a method of the process of computation called the cumulative method, it is based upon repeated cumulative process. The cumulative method is being adapted to the purposes of computation, particularly multiplication and division. The operations of multiplication and division are represented by algebraic formulas. An advantage of the method is that the cumulative process can be performed on decimal numbers. The present paper aims to establish a basic and useful formula valid for the two fundamental arithmetic operations of multiplication and division. The new cumulative method proved to be more flexible and made it possible to extend the multiplication and division based on repeated addition/subtraction to decimal numbers.
基金Postdoctoral Research Foundation of China (2021M700608)Natural Science Foundation Project of Chongqing, Chongqing Science and Technology Commission (cstc2021jcyj-bsh0047)+1 种基金Scientific Project Supported by the Bureau of Planning and Natural Resources, Chongqing (2301DH09002)Sichuan Transportation Science and Technology Project (2018ZL-01)。
文摘Landslide susceptibility assessment is an essential tool for disaster prevention and management. In areas with multiple fault zones, the impact of fault zone on slope stability cannot be disregarded. This study performed qualitative analysis of fault zones and proposed a zoning method to assess the landslide susceptibility in Chengkou County, Chongqing Municipality, China. The region within a distance of 1 km from the faults was designated as sub-zone A, while the remaining area was labeled as sub-zone B. To accomplish the assessment, a dataset comprising 388 historical landslides and 388 non-landslide points was used to train the random forest model. 10-fold cross-validation was utilized to select the training and testing datasets for the model. The results of the models were analyzed and discussed, with a focus on model performance and prediction uncertainty. By implementing the proposed division strategy based on fault zone, the accuracy, precision, recall, F-score, and AUC of both two sub-zones surpassed those of the whole region. In comparison to the results obtained for the whole region, sub-zone B exhibited an increase in AUC by 6.15%, while sub-zone A demonstrated a corresponding increase of 1.66%. Moreover, the results of 100 random realizations indicated that the division strategy has little effect on the prediction uncertainty. This study introduces a novel approach to enhance the prediction accuracy of the landslide susceptibility mapping model in areas with multiple fault zones.
基金Supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region,China,No.2023GXNSFAA026070 and No.2018GXNSFAA281071.
文摘BACKGROUND Cell division cyclin 25C(CDC25C)is a protein that plays a critical role in the cell cycle,specifically in the transition from the G2 phase to the M phase.Recent research has shown that CDC25C could be a potential therapeutic target for cancers,particularly for hepatocellular carcinoma(HCC).However,the specific regulatory mechanisms underlying the role of CDC25C in HCC tumorigenesis and development remain incompletely understood.AIM To explore the impact of CDC25C on cell proliferation and apoptosis,as well as its regulatory mechanisms in HCC development.METHODS Hepa1-6 and B16 cells were transduced with a lentiviral vector containing shRNA interference sequences(LV-CDC25C shRNA)to knock down CDC25C.Subsequently,a xenograft mouse model was established by subcutaneously injecting transduced Hepa1-6 cells into C57BL/6 mice to assess the effects of CDC25C knockdown on HCC development in vivo.Cell proliferation and migration were evaluated using a Cell Counting Kit-8 cell proliferation assays and wound healing assays,respectively.The expression of endoplasmic reticulum(ER)stress-related molecules(glucose-regulated protein 78,X-box binding protein-1,and C/EBP homologous protein)was measured in both cells and subcutaneous xenografts using quantitative real-time PCR(qRT-PCR)and western blotting.Additionally,apoptosis was investigated using flow cytometry,qRT-PCR,and western blotting.RESULTS CDC25C was stably suppressed in Hepa1-6 and B16 cells through LV-CDC25C shRNA transduction.A xenograft model with CDC25C knockdown was successfully established and that downregulation of CDC25C expression significantly inhibited HCC growth in mice.CDC25C knockdown not only inhibited cell proliferation and migration but also significantly increased the ER stress response,ultimately promoting ER stress-induced apoptosis in HCC cells.CONCLUSION The regulatory mechanism of CDC25C in HCC development may involve the activation of ER stress and the ER stress-induced apoptosis signaling pathway.
基金supported by the National Natural Science Foundation of China(42207529)the China Postdoctoral Science Foundation(2022M721289).
文摘Rice production is crucial for food security in China,and its relationship with rural labor migration has been studied extensively.Labor migration in rural China has taken new forms in recent years.There has been a discernible trend wherein adult children have started migrating to cities while their elderly parents return to villages to re-engage in on-farm work.The phenomenon has notably shaped the intergenerational division of labor(IDL)within households.However,it remains to be seen how farmers adjust their rice production systems in response to the IDL.The age of 60 years for employment injury insurance is the eligibility threshold for off-farm employment and is used to obtain a source of exogenous variation in the IDL.Based on a representative household survey of 1,752 rice farmers in the Hubei Province of Central China,our fuzzy regression discontinuity analysis reveals that farmers in IDL households are more likely to adopt ratoon rice(RR)than single cropping rice(SR)or double cropping rice(DR).The effect of the IDL varies under different levels of operational scales and specialized agricultural service availability.Further analysis suggests that farmers’arrangements are associated with two potential mechanisms of downward intergenerational transfer.Monetary transfer for urban housing purchases increases RR in IDL households,and time transfer for intergenerational childcare significantly promotes SR in IDL households.This study enhances the understanding of the relationship between rural labor migration and rice production in China,providing a reference for adjusting rice production systems to ensure food security.
基金The Petrochina Basic Prospective Science and Technology Research Project–Overseas deepwater ultra-deepwater oil and gas exploration technology research topic,under contract No.2021DJ2403。
文摘The Zambezi Delta basin is a passive marginal basin located on the East African coast that has good oil and gas exploration potential.Due to the special geological evolutionary background of the Beira High in the Zambezi Delta basin,it has a low gravity anomaly,and the existing seismic survey lines do not cover the whole basin;therefore,it is difficult to interpret the structural characteristics of the whole basin based solely on gravity or seismic data.Based on satellite altimetry gravity anomaly data,this study infers the distribution characteristics of faults in the Zambezi Delta basin by using the normalized vertical derivative of the total horizontal derivative(NVDR-THDR)technique.Then,constrained by seismic data,the gravity anomaly at the Moho interface is extracted by using the fast forward method of the double-interface model of the gravity anomaly,and this anomaly is then removed from the Bouguer gravity anomaly to obtain the sedimentary layer gravity anomaly.The thickness of the sedimentary strata is obtained by inversing the sedimentary basement depth of the whole basin.Then,uplifts and depressions are divided based on a sedimentary layer thickness of 3 km.This research demonstrates that the Zambezi Delta basin mainly features nearly SN-trending and NE-trending faults and that these faults exhibit east-west partitioning.The nearly SN-trending strike-slip faults controlled the sedimentary development of the basin,and the NE-trending tensile faults may have acted as migration channels for oil,gas and magma.The“overcompensation”effect of the Moho interface gravity anomaly on the gravity anomaly of the sedimentary layer is caused by the depression of the Moho interface beneath the Beira High,which results in a low gravity anomaly value for the Beira High.The pattern of uplifts and depressions trends NE and has the structural characteristics of east-west blocks.
文摘Administrative regions are an important environment for the operation of China's market economy.The relevant economic subjects cannot predict the policy of adjustment of administrative divisions and carry out conscious migration behavior,adjustment of administrative divisions can be regarded as a quasi-natural experiment.The three cities of Hefei,Wuhu,and Ma'anshan,which are directly related to the adjustment of the administrative division of Chaohu,are taken as the treatment group,and the seven adjacent cities of Lu'an,Huainan,Chuzhou,Bengbu,Anqing,Chizhou,and Tongling are taken as the control group.Differences-in-Differences method and relevant control variables affecting the upgrading of industrial structure are used to test.The test results show that"Partitions of Chaohu"has a significant industrial structure upgrading effect by promoting the optimization of spatial layout,the cross-regional flow of production factors and the effective management of Chaohu Lake Basin.At the same time,the increase of total retail sales of consumer goods,urban fixed assets investment,public utility expenses in science,education,culture and health,and population plays a significant positive role in promoting the upgrading of industrial structure,while foreign direct investment plays a certain inhibition role in the upgrading of industrial structure.In order to meet the ever-developing space demands and enhance the impact on surrounding areas,the Hefei metropolitan area should be driven by technological innovation,strengthen the integration of industrial chains,improve the business environment and transportation network,and continuously promote the upgrading of industrial structure and the formation and development of new productive forces.
文摘In this work, we present a general theoretical study leading to analytical expression of the seasonal temperature at the near surface that is expected to evaluate any area seasonal temperature of the world using the least square method to fit the hourly data to the theoretical curve of the temperature. It is shown that the temperature is globally the result of two contributions: the contribution of the revolution movement of the terrestrial globe on its elliptical orbit around the sun, the contribution of the spin-orbit coupling for the rotation movement of the terrestrial globe around its polar axis and its revolution movement. The orbital behavior of the temperature is used to find the seasonal divisions of the climate for the local area considered. The whole expression of the temperature is very useful for the meteorological needs. The contribution of the human activities and natural instabilities are the results of discrepancies which increase errors (standard deviations).
文摘As an important group of plant cellular organelles, the molecular mechanism of plastid division is poorly understood. Recent studies have revealed that the homologs of ftsZ gene, an essential prokaryotic cell division gene, are involved in plastid division process of plant cells. Antisense and sense expression constructions were employed to investigate the functions of the two ftsZ genes, NtFtsZ1 and NtFtsZ2, in transgenic Nicotiana tabacum L. plants. Although antisense expression of,NtFtsZs reduced the native protein level obviously, the size and number of chloroplasts in transgenic tobacco plants had no effect. In contrast, overexpression of NtFtsZs in transgenic plants strikingly changed the number and morphology of chloroplasts. Even only 1 - 2 huge chloroplasts could be seen in the mesophyll cells of some overexpression transgenic plants. Analyses of chloroplast ultrastructures and chlorophyll content of different transgenic plants suggested that NtFtsZs gene have no direct influence on the normal development and function of chloroplasts. ne changes in chloroplast morphology must be a compensation for the change in chloroplast number. The different phenotypes of chloroplasts in antisense and sense transgenic plants implied that different members from the same ftsZ gene family may have similar function in controlling plastid division. Meanwhile, the changes of chloroplast morphology in sense transgenic plants represented the possible plastoskeleton function of ftsZ in higher plant.
基金Supported by The Ministry of Finance,Meteorological Industry Spe-cific(GYHY200706030)China Meteorological Administration 2009 Budget Item(CMATG2009M32)~~
文摘The average rainfall in whole growth period of winter wheat to plus or minus several times of sample variance was used to define different grades of drought.The reduction rate of production in meteorological which was equal to or greater than the rate of 3% was taken as standards to define wheat drought disaster years.The average reduction ratio of production in the wheat drought years in Hebei-Shandong-Henan was calculated.The frequency distribution rule of wheat in different drought disaster strength of winter wheat in Hebei-Shandong-Henan was analyzed.According to the conception and calculation method of drought disaster risk index of winter wheat,the regional distribution law of drought disaster risk index of winter wheat was analyzed.The division technical methods of winter wheat drought disaster risk zones in Hebei-Shandong-Henan under the irrigated conditions were put forward.Taking the average reduction rate of production in drought years,drought disaster risk index and precipitation of winter wheat in growth periods as indices,using the statistical analysis and overlay function of GIS,the production reduction risk of winter wheat caused by drought disaster in winter wheat zones of Hebei-Shandong-Henan was divided and evaluated.The risk evaluation of production reduction of winter wheat in different risk zones under different climate conditions was realized.The disaster prevention and mitigation measures of winter wheat drought were given.
基金supported by the National Science and Technology Major Project(No.2011ZX05007-006)the 973 Program of China(No.2013CB228604)the major Project of Petrochina(No.2014B-0610)
文摘Ground roll waves interfere with seismic data. The suppression of ground roll waves based on the division of wavelet frequencies considers the low-frequency characteristics of ground roll waves. However, this method will not be effective when the ground roll wave and the effective signal have the same frequency bands because of overlapping. The radial trace transform (RTT) considers the apparent velocity difference between the effective signal and the ground roll wave to suppress the latter, but affects the low-frequency components of the former. This study proposes a ground roll wave suppression method by combining the wavelet frequency division and the RTT based on the difference between the ground roll wave velocity and the effective signal and their energy difference in the wavelet domain, thus making full use of the advantages of both methods. First, we decompose the seismic data into different frequency bands through wavelet transform. Second, the RTT and low-cut filtering are applied to the low-frequency band, where the ground roll waves are appearing. Third, we reconstruct the seismic record without ground roll waves by using the inverse RTT and the remaining frequency bands. The proposed method not only improves the ground roll wave suppression, but also protects the signal integrity. The numerical simulation and real seismic data processing results suggest that the proposed method has a strong ability to denoise while preserving the amplitude.
文摘Two plastid division genes, NtFtsZ1 and NtFtsZ2 isolated from Nicotiana tabacum L. were fused with gfp and expressed in Escherichia coli . The regular localizations of full length NtFtsZs∶GFP along the filamentous bacteria indicated that the NtFtsZs could recognize the potential division sites in E. coli and be polymerized with heterogeneous FtsZ from bacteria. The overexpression of NtFtsZs ∶ gfp inhibited the division of host strain cells and resulted in the long filamentous bacterial morphology. These results suggested that eukaryotic ftsZs have similar function to their prokaryotic homologs. Meanwhile, the different deletions of motifs of NtFtsZs are also employed to investigate the functions of these proteins in E. coli . The results showed that the C_terminal domains of NtFtsZs were related to the correct localization of NtFtsZs in E. coli and the N_terminal domains of NtFtsZs were responsible for the polymerization of homogeneous and heterogeneous FtsZ proteins. The significance of these results in understanding the functions of NtFtsZs in plastid division were discussed.
基金Supported by Notice on the First Batch of National Modern Agricultural Demonstration Zone by the Ministry of Agriculture([2010]22)the Research and Application Project for the Early Warning and Comprehensive Control of the Major Pests and Diseases for Main Grain and Oil Crops(N1997-ZC002)the Fundamental Research Funds for the Central Universities(XDJK2015C060,SWU114046,2362015xk04)~~
文摘With the aim to improve the level of monitoring and warning as well as the comprehensive control of rice blast disease, and to feasibly reduce the disease threat in Nanchong City, the methods of GPS and GIS, systematical monitoring and field survey, rice blast resistance identification, physiologic races of rice blast monitoring, and meteorological data analysis were performed to study the occurrence and epidemic region division, precise demarcation and occurrence and epidemic regularity of rice blast in Nanchong City. This study first completed the epidemic region division and precise demarcation; first clarified the initial affection(beginning period) locations, occurrence characteristics, epidemic trends and characteristics; explicated the existence of four epidemic peak periods of rice blast in the field, where the damage areas of first peak period played a decisive role during the blast epidemic years; in late May, the cumulative occurrence areas and annual occurrence areas presented higher positive correlation with the correlation coefficient of 0.817;and established a prediction model of occurrence areas per year based on the disease field rate at the end of boot stages and the diseased plant rate at dough stages. The results of investigation on the impact factors investigation of blast disease in Nanchong in recent years suggested that the internal causes were the decrease or loss of blast resistance of the rice cultivars, as well as the increase of physiological races with strong resistance to rice blast and the emergence of new virulent physiologic varieties; the external causes were suitable temperature, too much rainy, and sunlight shortage. Between 2010 and 2015, the short-term forecast accuracy for rice blast in Nanchong was up to 100%, and medium-and long-term forecast accuracy was also up to 98% and 95%, respectively, which increased by 5-15% than that before 1997, thereby making the control effect of rice blast in Nanchong increased by 15-30%.
基金Supported by the Project of Meteorological Service Website Construction for HainanBreeding(Qiong Nong Ji Cai No.[2013]18 of Hainan Provincial Department of Agriculture)~~
文摘Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding.