The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water ...The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water （WtF） from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl （DPPH） free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence （CL） assay and hydroxyl free radical-initiated chemiluminescence （CL） assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.展开更多
根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上...根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。展开更多
The Agrobacterium sp.HN1 was isolated from the soil and used to adsorb rare earth ions La3＋ and Ce3＋.The results showed that the pretreatments of Agrobacterium sp.HN1 did not enhance the adsorption of La3＋ and Ce3...The Agrobacterium sp.HN1 was isolated from the soil and used to adsorb rare earth ions La3＋ and Ce3＋.The results showed that the pretreatments of Agrobacterium sp.HN1 did not enhance the adsorption of La3＋ and Ce3＋.The pH,temperature,time and bacterial age affected the dynamics of adsorption of La3＋ and Ce3＋.The optimum adsorption conditions for Agrobacterium sp.HN1 were as follows：15 mg/L La3＋ of initial concentration,10 mg/L Ce3＋,300 mg/L（dry wt.cell） biomass for sorption,6.8 pH,30 oC temperature,150 r/min rotational speed,2 h adsorption time and 28 h bacterial age.The adsorption kinetics of La3＋ and Ce3＋ for Agrobacterium sp.HN1 followed the pseudo-second order equation.展开更多
Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan ...Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreosci/la hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide （CO） dif- ference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physi- ological changes included its elevated respiration rate and cellular invertase activity.展开更多
文摘The three water-soluble polysaccharides （PI, PII and Pill）, the ethyl acetate extraction （EA） and its five fractions of petroleum ether （PeF）, ethyl acetate （EaF）, butanol （BuF）, methanol （MeF） and water （WtF） from marine agrobacterium 1202 were prepared and then subjected to antioxidant activity evaluation. The antioxidant activity was tested using the 1, 1-Diphenyl-2-picryl-hydrazyl （DPPH） free radical-scavenging assay, the hydrogen peroxide-induced luminot chemiluminescence （CL） assay and hydroxyl free radical-initiated chemiluminescence （CL） assay. All the fractions exerted significant inhibitory effects on hydrogen peroxide and hydroxyl free radical. The extracts of EA, EaF and BuF could also showed inhibition on DPPH. Among of them, the extracts of EaF showed full of antioxidant activity on the three tested system. The result suggested that the marine agrobacterium 1202 is a potential source of natural antioxidantagent.
文摘根据《实施卫生与植物卫生措施协定》(Agreementon the Application of Sanitary and Phytosanitary Measures,简称《SPS协定》)中的透明度原则,成员应保证迅速公布所有拟采用的但尚没有对应的国际标准、指南或建议或与其在内容实质上不同的,且可能对其他成员贸易产生重大影响的卫生与植物卫生措施,以使利益相关方知晓。
基金supported by the Natural Science Research Program of Henan Province,China(2007180025)
文摘The Agrobacterium sp.HN1 was isolated from the soil and used to adsorb rare earth ions La3＋ and Ce3＋.The results showed that the pretreatments of Agrobacterium sp.HN1 did not enhance the adsorption of La3＋ and Ce3＋.The pH,temperature,time and bacterial age affected the dynamics of adsorption of La3＋ and Ce3＋.The optimum adsorption conditions for Agrobacterium sp.HN1 were as follows：15 mg/L La3＋ of initial concentration,10 mg/L Ce3＋,300 mg/L（dry wt.cell） biomass for sorption,6.8 pH,30 oC temperature,150 r/min rotational speed,2 h adsorption time and 28 h bacterial age.The adsorption kinetics of La3＋ and Ce3＋ for Agrobacterium sp.HN1 followed the pseudo-second order equation.
基金supported by the Fundamental Research Funds for the Central Universities of China(No.30920130121013)the National Natural Science Foundation of China(No.31300111)
文摘Salecan is a novel exopolysaccharide produced by the strain Agrobacterium sp. 7__X09, and it is composed of only glucose monomers. The unique chemical composition and excellent physicochemical properties make Salecan a promising material for applications in coagulation, lubrication, protection against acute liver injury, and alleviating constipation. In this study, we cloned the Vitreosci/la hemoglobin gene into a broad-host-range plasmid pCM158. Without antibiotic selection, there was negligible loss of the plasmid in the host Agrobacterium sp. ZX09 after one passage of cultivation. The expression of Vitreoscilla hemoglobin was demonstrated by carbon monoxide （CO） dif- ference spectrum. The engineered strain Agrobacterium sp. ZX09 increased Salecan yield by 30%. The other physi- ological changes included its elevated respiration rate and cellular invertase activity.
