Effects of Some Materials Extracted from Ajuga Species on the Larvae of Hyphantria cunea and its Natural Enemies

Six kinds of cxtracts drawn from Ajuga multiflora and .A. multliflora var. brevispicala and A. multiflora var.serotina using methanol and acetone were used in this experiment to test their killing activity to the second instar larvae ofHypantria cunea and their influence on its natural enemics. The average death rate caused by those extracts on the secondinstar larvae was 85.70%. The mortality rate caused by the extracts drawn with methanol was from 88.89% to 96.33%,which was significal1tly l1igl1er tl1a11 tl1at caused by acetol1e extracts. 'I11osc extracts were satt to TrchogranInIa `Ie)uI,xiIinIi,Coccinella septempunctata, the natural enelnies of H. cunea. We did not found any evidence shown that those extracts hadany influence the emergence and the devclopment of T. dendrolimi. Those methanolic extracts gotten from A. multiflora andA. multiflora var. brevispicata had no significant effects on the mortality of the larvae and adults of C. septenrpunctala.Those eXtracts could be used in the control of H cunea sattly.

多花筋骨草在郑州市园林景观中的应用效果初报

为增加郑州市园林地被植物的应用范围,在郑州市开展了多花筋骨草的引种栽培技术研究,结果表明:多花筋骨草在郑州地区可粗放管理,整体表现良好,是一种喜光又耐阴,耐寒、耐热性强,抗性强,繁殖速度快且人工繁殖成活率高的优秀多年生常绿地被植物,观赏效果良好,既可观花又可观叶且花期长,群开效果好,适宜在郑州市园林景观中大量应用。

筋骨草内蜕皮激素类似物对一些刺吸式口器昆虫及其天敌的影响

提取多花筋骨草、多花筋骨草短穗变种、多花筋骨草莲座变种和线叶筋骨草蜕皮激素类似物，稀释成一定浓度，将新鲜枝条在提取液中培养，而后在室内接种桃蚜、柳沫蝉、糖槭盔蚧等，昆虫通过刺吸取食后，对其生命活动产生很大影响。多数提取液的２５或５０倍液对柳沫蝉的杀虫率都在９０％以上，但对其寄生性小蜂的羽化率和捕食性天敌异色瓢虫存活状况影响不大。

两种筋骨草中蜕皮激素类物质的分析

对两种筋骨草甲醇提取物进行了分离、分析，确定了其中含有的蜕皮激素类物质主要为２０－ＯＨｅｃｄｙｓｏｎｅ、Ｃｙａｓｔｅｒｏｎ及少量２０－ＯＨｅｃｄｙｓｏｎｅ－３－ａｃｅｔａｔｅ、２０－ＯＨｅｃｄｙｓｏｎｅ－２－ａｃｅｔａｔｅ、Ａｊｕｙａｌａｅｔｏｎｅ，并测得了各物质含量。

筋骨草提取液对几种鳞翅目幼虫的杀虫效果

为确定蜕皮激素对昆虫的生长、发育、繁殖的影响，利用多花筋骨草、多花筋骨草短穗变种、多花筋骨草莲座变种和线叶筋骨草提取液，采取浸枝叶饲养舞毒蛾、天幕毛虫和树粉蝶幼虫的方法进行了杀虫效果试验。结果表明：对其生长发育有较大的影响，多数提取液对这些幼虫的杀虫效果均在９０％以上。

多花筋骨草的显微鉴定

目的:为准确鉴定药材,保证用药安全有效,并进一步制定多花筋骨草的质量标准及开发利用提供科学依据。方法:石蜡切片技术及数码显微成像技术。结果:明确了多花筋骨草根、根茎及叶的显微特征。结论:这些显微特征可作为多花筋骨草的鉴定及区别同科同属植物的依据。

多花筋骨草高频再生体系的建立

为了建立一个高效的多花筋骨草组织培养再生体系,以多花筋骨草嫩叶为外植体,进行了不同激素配比对愈伤组织诱导,不定芽增殖及根诱导.结果表明:6-BA,KT及2,4-D组合从外植体诱导出愈伤;6-BA和NAA组合可从外植体诱导出苗;6-BA可诱导不定芽及不定芽增殖;IBA可诱导根系发生.

多花筋骨草提取物抗氧化活性研究

目的:采用体外抗氧化实验,对多花筋骨草醇提物的抗氧化活性进行研究,为多花筋骨草的开发和应用提供参考数据。方法:用甲醇冷浸提取多花筋骨草,减压浓缩得到多花筋骨草醇提物,采用1,1-二苯基-2-三硝基苯肼(DPPH·)法、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS+)法和总还原能力测定法,研究其提取物的抗氧化活性。结果:多花筋骨草醇提物对DPPH·、ABTS+均有清除活性,以及较强还原能力,且DPPH·清除活性(IC50值)为0.348 mg/mL、ABTS+清除活性(IC50值)为0.292 mg/mL,总还原能力(IC50值)为2.736 mg/mL。结论:多花筋骨草醇提物有较强的抗氧化活性,且抗氧化活性与醇提物质量浓度两者间存在一定量效关联。