The End of the Road is John Barth's second novel, and it is the first novel that brought abortion to the focus of public attention, which proves to be a huge success. This paper intends to study the alienation in ...The End of the Road is John Barth's second novel, and it is the first novel that brought abortion to the focus of public attention, which proves to be a huge success. This paper intends to study the alienation in The End of the Road based on the alienation as a literary style and the alienation embodied in the literary context. Through the analysis of the alienated plot, the paper aims to emphasize the internal cause for the end of the different character's road.展开更多
“Alienated labor”came from Marx’s Manuscript of Philosophy and Economics in 1844.This theory was formed on the basis of Marx’s critical inheritance of predecessors’thoughts on alienation and the reality under the...“Alienated labor”came from Marx’s Manuscript of Philosophy and Economics in 1844.This theory was formed on the basis of Marx’s critical inheritance of predecessors’thoughts on alienation and the reality under the capitalist system.It is of great significance to understand the final formation of Marx’s historical materialism.展开更多
Alienated labor, also known as labor alienation, was a concept with historical creativity, which was first raised in Marx' s Economic and Philosophical Manuscripts of 1844. The Manuscripts was important works of Marx...Alienated labor, also known as labor alienation, was a concept with historical creativity, which was first raised in Marx' s Economic and Philosophical Manuscripts of 1844. The Manuscripts was important works of Marx, in which the social relations of capitalism private ownership were analyzed by Marx using the concept of alienated labor. A deep analysis was conducted by Marx from the specific historical facts, in which the rational kernel of the alienated problem in the social relations of capitalism was mentioned. Specifically, the alienated labor theory was analyzed from the relationship of laborers with their labor products and labor itself, human' s essence, and other people under the condition of the capitalist private ownership; the "ought to be" ethical characteristics of labors were expounded from the perspective of ethics, and the abnormal labor relations in the process of capitalist production were ethically reviewed and criticized. Therefore, rich economic ethics thoughts were contained in Marx' s analysis of the alienated labor theory.展开更多
J. D. Salinger, American novelist and short story writer, is recognized by critics and readers alike as one of the most popular and influential of contemporary writers.His only novel,"The Catcher in the Rye"...J. D. Salinger, American novelist and short story writer, is recognized by critics and readers alike as one of the most popular and influential of contemporary writers.His only novel,"The Catcher in the Rye",so vividly captured the spirit of the fifties and early sixties that those years have been called"The Age of Holden Caulfield."Salinger draws a rich psychological portrait of Holden Caulfield through vivid, colloquial style,using slang and ungrammatical or sloppy pattern of speech. Drawing on an analysis of Salinger's life and his age,this paper seeks to explore Salinger's portrayal of a disillusioned youth and the exposure of the fraudulence of contemporary America in combination with his manipulation of colloquial style and dramatization of interior monologue.展开更多
Relict subtropical coniferous forests in China face severe fragmentation,resulting in declining populations,and some are under significant threat from invasive alien species.Despite the crucial importance of understan...Relict subtropical coniferous forests in China face severe fragmentation,resulting in declining populations,and some are under significant threat from invasive alien species.Despite the crucial importance of understanding forest dynamics,knowledge gaps persist,particularly regarding the impact of invasive plants on vulnerable natives like Keteleeria evelyniana.In this study,we investigated the impact of invasive plants on the regeneration of forests dominated by K.evelyniana,a subtropical relict species in southwestern China.For this purpose,we characterized forest dynamics of 160 forest plots featuring K.evelyniana as the primary dominant species and determined whether the presence of invasive plants was correlated with regeneration of K.evelyniana.We identified four distinct forest types in which K.evelyniana was dominant.We found that radial growth of K.evelyniana trees is faster in younger ageclasses today than it was for older trees at the same age.The population structure of K.evelyniana in each forest type exhibited a multimodal age-class distribution.However,three forest types lacked established saplings younger than 10 years old,a situation attributed to the dense coverage of the invasive alien Ageratina adenophora.This invasive species resulted in a reduction of understory species diversity.Additionally,our analysis uncovered a significant negative correlation in phylogenetic relatedness(net relatedness index)between native and invasive alien plant species in eastern Yunnan.This suggests closely related invasive species face heightened competition,hindering successful invasion.Taken together,our findings indicate that successful establishment and habitat restoration of K.evelyniana seedling/saplings require effective measures to control invasive plants.展开更多
Biological invasion is triggered by human development activities such as the construction and expansion of road networks.Road verges serve as important habitats and corridors for the distribution of invasive alien pla...Biological invasion is triggered by human development activities such as the construction and expansion of road networks.Road verges serve as important habitats and corridors for the distribution of invasive alien plant species(IAPS)between geographically distant habitats.However,the trajectory of plant invasion and the data regarding the impact of roads on IAPS distribution are relatively poor in Nepal.Here,we surveyed two road types(main roads and feeder road)in the Middle Mountain region of central Nepal in order to investigate how different road types are driving the dispersal of IAPS along road verges and the adjacent natural habitats.Systematic sampling was conducted at ca 2.5 km intervals along the roads.At each sampling site,paired plots(25 m×4 m)were sampled:one adjacent to and along the road,and another 20 m away and parallel to it in the interior habitat.Our results revealed that the main road verges had a higher cover(33%)and a larger number of IAPS(14 species)than the feeder road(25%;10 species).The IAPS cover and richness were significantly higher along verges than in the adjacent interior habitats for both road types,indicating that roads are contributing as corridors for the dispersal of IAPS in the Middle Mountain areas of central Nepal.Further,elevation,tree canopy,and disturbances(grazing/mowing/trampling)were found to be the key factors that determine spatial distribution of IAPS along road verges.We emphasize that regular monitoring of vegetation along the road verges can help with the early detection and control of potential IAPS in the region before they become problematic.展开更多
Charismatic species are often reported by the media,providing information for detecting population status and public perception.To identify the number and distribution of free-living Black Swan(Cygnus atratus),a chari...Charismatic species are often reported by the media,providing information for detecting population status and public perception.To identify the number and distribution of free-living Black Swan(Cygnus atratus),a charismatic alien species in Chinese mainland and to detect the public and the media attitudes to the species,we analyzed the reports and emotional tendency from media coverage in 2000-2022 using manual reading,crawler extraction and latent Dirichlet allocation.A total of 6654 Black Swans were reported at 711 sites,including 147 individuals at 30 nature reserves.Successful breeding was reported at one-fourth of the total sites,including five nature reserves.The proportion of positive emotional tendency to Black Swans was overwhelming in the reports and was higher than that to alien species in general,suggesting that the public and the media are unaware of the risk of biological invasion.Effective management of invasive species requires the media clarifies the invasion risk of charismatic alien species.Promoting the unity between the harmfulness of abstract concept of alien species and the charisma of a specific alien species among the public help effective management.展开更多
Based on the biological characteristics of Solenopsis invicta and the structural characteristics of its ant nest,a fast and efficient closed treatment device was developed.Compared with the simple chemical treatment c...Based on the biological characteristics of Solenopsis invicta and the structural characteristics of its ant nest,a fast and efficient closed treatment device was developed.Compared with the simple chemical treatment commonly used at present,the developed treatment device(the ant nest control cover)is a fast and efficient method to exterminate S.invicta in 7 d,featured by short course,quick results and good effect.展开更多
In the poems created by the renowned American poet Emily Dickinson,those related to nature constitute a considerable proportion.However,Dickinson’s attitude toward nature is,in fact,contradictory.On the one hand,Dick...In the poems created by the renowned American poet Emily Dickinson,those related to nature constitute a considerable proportion.However,Dickinson’s attitude toward nature is,in fact,contradictory.On the one hand,Dickinson perceives nature as gentle and kind,akin to a mother.Nature is regarded as“heaven”and embodies harmony.On the other hand,nature possesses its own operational principles and harbors inherent cruelty.According to Dickinson,humans should refrain from excessive interference in the natural order.This article attempts to analyze the similarities in attitude towards nature between Dickinson’s poetry and Taoist philosophy.展开更多
The Last Quarter of the Moon is a novel written by Chi Zijian,which describes the life of the Ewenki ethnic minority in northeast China.The novel has received wide attention for its profound theme and unique narrative...The Last Quarter of the Moon is a novel written by Chi Zijian,which describes the life of the Ewenki ethnic minority in northeast China.The novel has received wide attention for its profound theme and unique narrative style.Defamiliarization is a technique commonly used in literary creation.By breaking the conventional expression mode and changing the conventional view,readers can have new feelings and understandings.The original text of The Last Quarter of the Moon describes the life of the Ewenki minority in China,which has a strong defamiliarization effect.In the English version,the translator adopts various methods to reproduce this defamiliarization.Through the analysis of its English translation,this paper discusses the defamiliarization embodied in it and the two translation methods used by the translator to reproduce the defamiliarization of the original text:alienizing translation and hybridization.It is found that the translation successfully retains the unique charm of the original text by means of language and cultural defamiliarization,enabling English readers to obtain a new reading experience,and revealing the important reasons why the English translation of The Last Quarter of the Moon can be successfully translated and spread in the Western world.展开更多
The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6V...The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6VS was microdissected with a needle and transferred into a 0.5 mL Ep tube. In the 'single tube', all the subsequence steps were conducted. After two round of LA (Linker adaptor)_PCR amplification, the size of PCR bands ranged from 100 to 3 000 bp, with predominate bands 600-1 500 bp. The products were confirmed by Southern blotting analysis using Haynaldia villosa (L.) Schur. genomic DNA labeled with 32 P as probe. The PCR products were purified and ligated into clone vector-pGEM_T easy vector. Then, the plasmids were transformed into competence E. coli JM109 with cool CaCl 2. It was estimated that there were more than 17 000 white clones in the library. The size of insert fragments distributed from 100-1 500 bp, with average of 600 bp. Using H. villosa genomic DNA as probe, dot blotting results showed that 37% clones displayed strong and medium positive signals, and 63% clones had faint or no signals. It is demonstrated that there were about 37% repeat sequence clones and 67% single/unique sequence clones in the library. Eight H. villosa_specific clones were screened from the library, and two clones pHVMK22 and pHVMK134 were used for RFLP analysis and sequencing. Both of them were H. villosa specific clones. The pHVMK22 was a unique sequence clone, and the pHVMK134 was a repeat sequence clone. When the pHVMK22 was used as a probe for Southern hybridization, all the powdery mildew resistance materials showed a special band of 2 kb, while all the susceptible ones not. The pHVMK22 may be applied to detect the existence of Pm21.展开更多
[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling pl...[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling plots investigation and route survey,and the whole Dongjiang River was covered.52 sampling points were set in the main streams,and 87 sampling points in tributaries streams,624 quadrats were set in total;multi-index comprehensive evaluation method was adopted for the risk assessment with 6 first-level indexes and 25 second-level indexes.[Result] Through the investigation on the riparian zone of Dongjiang River for 2 years,51 species of alien invasive plants were found,belonging to 17 families and 38 genera.The risk level assessment results of these 51 species showed that 18 species,such as Ageratum conyzoides,belonged to high-level risk with over 60 points;26 species,such as Chenopodium ambrosioides,were between 30 and 60 points which belonged to middle-level risk;7 species,such as Paspalum dilatatum,with less than 30 points belonged to low-level risk.[Conclusion] Basic data on the protection,water environmental management and functional zoning of riparian zone of Dongjiang River were provided.展开更多
异己手综合征(alien hand syndrome,AHS)是一种复杂的临床表现各异的现象,是以患手不受患者意愿支配,或误把患肢当作外人肢体,以及可观察到的非意愿性肢体活动为主要特征的临床综合征^([1])。通常被界定为一侧上肢无意愿的(unwille...异己手综合征(alien hand syndrome,AHS)是一种复杂的临床表现各异的现象,是以患手不受患者意愿支配,或误把患肢当作外人肢体,以及可观察到的非意愿性肢体活动为主要特征的临床综合征^([1])。通常被界定为一侧上肢无意愿的(unwilled)、不可控的(uncontrollable)但看似有目的的(purposeful)动作^([2])。展开更多
[Objective]This study was to reveal the essence of mechanism about how the alien invasive plants spread.[Method]Species niche and material/energy flow were used as basic research indicators to analyze the intrinsic me...[Objective]This study was to reveal the essence of mechanism about how the alien invasive plants spread.[Method]Species niche and material/energy flow were used as basic research indicators to analyze the intrinsic mechanism of alien plants invasion.[Result]Most of the invasive plants have not been explicitly defined and their effective control methods not brought forward.[Conclusion]Overrun of alien invasive plants depends on whether the niche of a species could be continuously met at spatial level.Based on this we put forward corresponding control measures,proposed an assumption to establish a cylinder-network model and discussed the definition of alien invasive plants.展开更多
[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition lin...[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition line were studied.[Method] The phenotype analysis was used to study the separation proportion of progeny.Moreover,SSR and the methylation analysis were used to study the transmission behavior of nonhomologous chromosome.[Result] 78 plants of 145 backcross progenies preserved the rolled leaf mark trait of MAAL8.In 32 anther culture plants,five plants had the normal rolled leaves,and two plants had the extremely rolled leaves.The rest had the flat leaves.14 couples of SSR markers were used to analyze,and it indicated that all the rolled-leaf plants could obtain the characteristic band type of O.officinalis,but the flat-leaf plants showed none of them.11 polymorphic RFLP markers were used to carry out Methylation-Sensitive Southern analysis.It showed that the methylation variation manners of eight markers between AA and CC genomes were different.[Conclusion] The nonhomologous chromosome of MAAL8 could pass to the progenies independently and integrally via the meiosis,and the phenotype characteristics didn't change.Moreover,the methylation manner of O.officinalis could inherit stably in the hybrid progeny as the addition of single chromosome.The stability of methylation might have the certain effect on the relatively independent inheritance of nonhomologous chromosome in the genome environment of O.sativa.展开更多
The situation of intercepted alien pests from imported hides in Hebei during 2013-2014 was systematically analyzed. The results showed that 163 batches of alien pests were intercepted, including 2 batches of quarantin...The situation of intercepted alien pests from imported hides in Hebei during 2013-2014 was systematically analyzed. The results showed that 163 batches of alien pests were intercepted, including 2 batches of quarantine weeds and they both were Italian cocklebur. According to the statistics of original countries, the intercepted hides from 21 countries all had alien pests, in which Australia was the largest number of batches and species of alien pests. The analysis results showed that there was severe risk in the imported hides carrying alien pests, thus to effectively prevent the invasion of alien pests to our country, several quarantine measures should be taken in the future, such as enhancing the quarantine inspection in ports, and promoting the personnel ability of interception and species identification of alien pests.展开更多
异己手综合征(alien hand syndrome,AHS)是由不同中枢神经损害引起的复杂的临床综合征,主要特点为患侧上肢或手不受意识支配,双手执行动作时存在明显地不协调或拮抗[1]。近年来国内报道较少,加之该病的临床表现复杂多样,症状辨别非常...异己手综合征(alien hand syndrome,AHS)是由不同中枢神经损害引起的复杂的临床综合征,主要特点为患侧上肢或手不受意识支配,双手执行动作时存在明显地不协调或拮抗[1]。近年来国内报道较少,加之该病的临床表现复杂多样,症状辨别非常困难,故非常容易漏诊、误诊。现将我院收治的一例AHS患者报道如下。1临床资料患者男性,77岁,右利手,展开更多
This thesis,by analyzing the wolf nature,the dog nature and the human nature,and their relations,expounds the author's personal interpretation of the theme of Sherwood Anderson's short story Death in the Woods...This thesis,by analyzing the wolf nature,the dog nature and the human nature,and their relations,expounds the author's personal interpretation of the theme of Sherwood Anderson's short story Death in the Woods.The author maintains that Anderson reveals to readers the ugliness of life and the strange beauty of life,condemns the loss of love and the dehumanization of human life in the modern world and implies Anderson's deep concern on the human alienation,an important theme in the 20th century modern literature.展开更多
文摘The End of the Road is John Barth's second novel, and it is the first novel that brought abortion to the focus of public attention, which proves to be a huge success. This paper intends to study the alienation in The End of the Road based on the alienation as a literary style and the alienation embodied in the literary context. Through the analysis of the alienated plot, the paper aims to emphasize the internal cause for the end of the different character's road.
文摘“Alienated labor”came from Marx’s Manuscript of Philosophy and Economics in 1844.This theory was formed on the basis of Marx’s critical inheritance of predecessors’thoughts on alienation and the reality under the capitalist system.It is of great significance to understand the final formation of Marx’s historical materialism.
文摘Alienated labor, also known as labor alienation, was a concept with historical creativity, which was first raised in Marx' s Economic and Philosophical Manuscripts of 1844. The Manuscripts was important works of Marx, in which the social relations of capitalism private ownership were analyzed by Marx using the concept of alienated labor. A deep analysis was conducted by Marx from the specific historical facts, in which the rational kernel of the alienated problem in the social relations of capitalism was mentioned. Specifically, the alienated labor theory was analyzed from the relationship of laborers with their labor products and labor itself, human' s essence, and other people under the condition of the capitalist private ownership; the "ought to be" ethical characteristics of labors were expounded from the perspective of ethics, and the abnormal labor relations in the process of capitalist production were ethically reviewed and criticized. Therefore, rich economic ethics thoughts were contained in Marx' s analysis of the alienated labor theory.
文摘J. D. Salinger, American novelist and short story writer, is recognized by critics and readers alike as one of the most popular and influential of contemporary writers.His only novel,"The Catcher in the Rye",so vividly captured the spirit of the fifties and early sixties that those years have been called"The Age of Holden Caulfield."Salinger draws a rich psychological portrait of Holden Caulfield through vivid, colloquial style,using slang and ungrammatical or sloppy pattern of speech. Drawing on an analysis of Salinger's life and his age,this paper seeks to explore Salinger's portrayal of a disillusioned youth and the exposure of the fraudulence of contemporary America in combination with his manipulation of colloquial style and dramatization of interior monologue.
基金funded by the Major Program for Basic Research Project of Yunnan Province,China,grant number 202101BC070002the Special Foundation for National Science and Technology Basic Resources Investigation of China,grant number 2019FY202300.
文摘Relict subtropical coniferous forests in China face severe fragmentation,resulting in declining populations,and some are under significant threat from invasive alien species.Despite the crucial importance of understanding forest dynamics,knowledge gaps persist,particularly regarding the impact of invasive plants on vulnerable natives like Keteleeria evelyniana.In this study,we investigated the impact of invasive plants on the regeneration of forests dominated by K.evelyniana,a subtropical relict species in southwestern China.For this purpose,we characterized forest dynamics of 160 forest plots featuring K.evelyniana as the primary dominant species and determined whether the presence of invasive plants was correlated with regeneration of K.evelyniana.We identified four distinct forest types in which K.evelyniana was dominant.We found that radial growth of K.evelyniana trees is faster in younger ageclasses today than it was for older trees at the same age.The population structure of K.evelyniana in each forest type exhibited a multimodal age-class distribution.However,three forest types lacked established saplings younger than 10 years old,a situation attributed to the dense coverage of the invasive alien Ageratina adenophora.This invasive species resulted in a reduction of understory species diversity.Additionally,our analysis uncovered a significant negative correlation in phylogenetic relatedness(net relatedness index)between native and invasive alien plant species in eastern Yunnan.This suggests closely related invasive species face heightened competition,hindering successful invasion.Taken together,our findings indicate that successful establishment and habitat restoration of K.evelyniana seedling/saplings require effective measures to control invasive plants.
文摘Biological invasion is triggered by human development activities such as the construction and expansion of road networks.Road verges serve as important habitats and corridors for the distribution of invasive alien plant species(IAPS)between geographically distant habitats.However,the trajectory of plant invasion and the data regarding the impact of roads on IAPS distribution are relatively poor in Nepal.Here,we surveyed two road types(main roads and feeder road)in the Middle Mountain region of central Nepal in order to investigate how different road types are driving the dispersal of IAPS along road verges and the adjacent natural habitats.Systematic sampling was conducted at ca 2.5 km intervals along the roads.At each sampling site,paired plots(25 m×4 m)were sampled:one adjacent to and along the road,and another 20 m away and parallel to it in the interior habitat.Our results revealed that the main road verges had a higher cover(33%)and a larger number of IAPS(14 species)than the feeder road(25%;10 species).The IAPS cover and richness were significantly higher along verges than in the adjacent interior habitats for both road types,indicating that roads are contributing as corridors for the dispersal of IAPS in the Middle Mountain areas of central Nepal.Further,elevation,tree canopy,and disturbances(grazing/mowing/trampling)were found to be the key factors that determine spatial distribution of IAPS along road verges.We emphasize that regular monitoring of vegetation along the road verges can help with the early detection and control of potential IAPS in the region before they become problematic.
基金financially supported by the National Key Research and Development Program of China(Number 2022YFC2601100)。
文摘Charismatic species are often reported by the media,providing information for detecting population status and public perception.To identify the number and distribution of free-living Black Swan(Cygnus atratus),a charismatic alien species in Chinese mainland and to detect the public and the media attitudes to the species,we analyzed the reports and emotional tendency from media coverage in 2000-2022 using manual reading,crawler extraction and latent Dirichlet allocation.A total of 6654 Black Swans were reported at 711 sites,including 147 individuals at 30 nature reserves.Successful breeding was reported at one-fourth of the total sites,including five nature reserves.The proportion of positive emotional tendency to Black Swans was overwhelming in the reports and was higher than that to alien species in general,suggesting that the public and the media are unaware of the risk of biological invasion.Effective management of invasive species requires the media clarifies the invasion risk of charismatic alien species.Promoting the unity between the harmfulness of abstract concept of alien species and the charisma of a specific alien species among the public help effective management.
基金Science and Technology Research Program of Xiamen Customs(2020XK08).
文摘Based on the biological characteristics of Solenopsis invicta and the structural characteristics of its ant nest,a fast and efficient closed treatment device was developed.Compared with the simple chemical treatment commonly used at present,the developed treatment device(the ant nest control cover)is a fast and efficient method to exterminate S.invicta in 7 d,featured by short course,quick results and good effect.
文摘In the poems created by the renowned American poet Emily Dickinson,those related to nature constitute a considerable proportion.However,Dickinson’s attitude toward nature is,in fact,contradictory.On the one hand,Dickinson perceives nature as gentle and kind,akin to a mother.Nature is regarded as“heaven”and embodies harmony.On the other hand,nature possesses its own operational principles and harbors inherent cruelty.According to Dickinson,humans should refrain from excessive interference in the natural order.This article attempts to analyze the similarities in attitude towards nature between Dickinson’s poetry and Taoist philosophy.
基金The study reported here was supported by the Zhejiang Social Science Planning Project(Fund No.22NDJC046YB).
文摘The Last Quarter of the Moon is a novel written by Chi Zijian,which describes the life of the Ewenki ethnic minority in northeast China.The novel has received wide attention for its profound theme and unique narrative style.Defamiliarization is a technique commonly used in literary creation.By breaking the conventional expression mode and changing the conventional view,readers can have new feelings and understandings.The original text of The Last Quarter of the Moon describes the life of the Ewenki minority in China,which has a strong defamiliarization effect.In the English version,the translator adopts various methods to reproduce this defamiliarization.Through the analysis of its English translation,this paper discusses the defamiliarization embodied in it and the two translation methods used by the translator to reproduce the defamiliarization of the original text:alienizing translation and hybridization.It is found that the translation successfully retains the unique charm of the original text by means of language and cultural defamiliarization,enabling English readers to obtain a new reading experience,and revealing the important reasons why the English translation of The Last Quarter of the Moon can be successfully translated and spread in the Western world.
基金国家"8 6 3"计划资助项目 (Z 17 0 4 0 1) 国家转基因植物研究与产业化资助项目 (J0 0 A 0 0 2 )~~
文摘The material T240_6 derived from SC 2 young embryo of the combination CA9211/RW15 (6D/6V alien substitution) was telosomic substitution line of 6VS identified by GISH (genomic in situ hybridization) analysis. The 6VS was microdissected with a needle and transferred into a 0.5 mL Ep tube. In the 'single tube', all the subsequence steps were conducted. After two round of LA (Linker adaptor)_PCR amplification, the size of PCR bands ranged from 100 to 3 000 bp, with predominate bands 600-1 500 bp. The products were confirmed by Southern blotting analysis using Haynaldia villosa (L.) Schur. genomic DNA labeled with 32 P as probe. The PCR products were purified and ligated into clone vector-pGEM_T easy vector. Then, the plasmids were transformed into competence E. coli JM109 with cool CaCl 2. It was estimated that there were more than 17 000 white clones in the library. The size of insert fragments distributed from 100-1 500 bp, with average of 600 bp. Using H. villosa genomic DNA as probe, dot blotting results showed that 37% clones displayed strong and medium positive signals, and 63% clones had faint or no signals. It is demonstrated that there were about 37% repeat sequence clones and 67% single/unique sequence clones in the library. Eight H. villosa_specific clones were screened from the library, and two clones pHVMK22 and pHVMK134 were used for RFLP analysis and sequencing. Both of them were H. villosa specific clones. The pHVMK22 was a unique sequence clone, and the pHVMK134 was a repeat sequence clone. When the pHVMK22 was used as a probe for Southern hybridization, all the powdery mildew resistance materials showed a special band of 2 kb, while all the susceptible ones not. The pHVMK22 may be applied to detect the existence of Pm21.
基金Supported by National Water Pollution Control and Management Technology Major Project of ChinaEcological Function Partition Research of Level Three and Level Four in Key Watershed(2012ZX07501002)the Fundamental Work Project of Ministry ofScience and Technology (2006FY111000)~~
文摘[Objective] The aim was to conduct investigation and risk assessment of the alien invasive plants in riparian zone of Dongjiang River.[Method] The field survey was carried out by the combination methods of sampling plots investigation and route survey,and the whole Dongjiang River was covered.52 sampling points were set in the main streams,and 87 sampling points in tributaries streams,624 quadrats were set in total;multi-index comprehensive evaluation method was adopted for the risk assessment with 6 first-level indexes and 25 second-level indexes.[Result] Through the investigation on the riparian zone of Dongjiang River for 2 years,51 species of alien invasive plants were found,belonging to 17 families and 38 genera.The risk level assessment results of these 51 species showed that 18 species,such as Ageratum conyzoides,belonged to high-level risk with over 60 points;26 species,such as Chenopodium ambrosioides,were between 30 and 60 points which belonged to middle-level risk;7 species,such as Paspalum dilatatum,with less than 30 points belonged to low-level risk.[Conclusion] Basic data on the protection,water environmental management and functional zoning of riparian zone of Dongjiang River were provided.
文摘异己手综合征(alien hand syndrome,AHS)是一种复杂的临床表现各异的现象,是以患手不受患者意愿支配,或误把患肢当作外人肢体,以及可观察到的非意愿性肢体活动为主要特征的临床综合征^([1])。通常被界定为一侧上肢无意愿的(unwilled)、不可控的(uncontrollable)但看似有目的的(purposeful)动作^([2])。
基金Supported by Joint Research Fund from National Natural Science Foundation of China(NSFC)-Yunnan Province(U0933601)Students Research Fund from Southwest Forestry University(1001)~~
文摘[Objective]This study was to reveal the essence of mechanism about how the alien invasive plants spread.[Method]Species niche and material/energy flow were used as basic research indicators to analyze the intrinsic mechanism of alien plants invasion.[Result]Most of the invasive plants have not been explicitly defined and their effective control methods not brought forward.[Conclusion]Overrun of alien invasive plants depends on whether the niche of a species could be continuously met at spatial level.Based on this we put forward corresponding control measures,proposed an assumption to establish a cylinder-network model and discussed the definition of alien invasive plants.
基金Supported by Hubei Natural Science Fund Item(2008CDB392)Natural Science Fund of South-Central University for Nationalities(yzz08005)~~
文摘[Objective] By carrying out the anther culture on the monosomic alien addition line MAAL8 of O.officinalis-O.sativa and the back crossing with O.sativa H1493,the genetic characteristics of monosomic alien addition line were studied.[Method] The phenotype analysis was used to study the separation proportion of progeny.Moreover,SSR and the methylation analysis were used to study the transmission behavior of nonhomologous chromosome.[Result] 78 plants of 145 backcross progenies preserved the rolled leaf mark trait of MAAL8.In 32 anther culture plants,five plants had the normal rolled leaves,and two plants had the extremely rolled leaves.The rest had the flat leaves.14 couples of SSR markers were used to analyze,and it indicated that all the rolled-leaf plants could obtain the characteristic band type of O.officinalis,but the flat-leaf plants showed none of them.11 polymorphic RFLP markers were used to carry out Methylation-Sensitive Southern analysis.It showed that the methylation variation manners of eight markers between AA and CC genomes were different.[Conclusion] The nonhomologous chromosome of MAAL8 could pass to the progenies independently and integrally via the meiosis,and the phenotype characteristics didn't change.Moreover,the methylation manner of O.officinalis could inherit stably in the hybrid progeny as the addition of single chromosome.The stability of methylation might have the certain effect on the relatively independent inheritance of nonhomologous chromosome in the genome environment of O.sativa.
基金Supported by the Scientific Research Project of Hebei Entry-Exit Inspection and Quarantine Bureau(HE2013K030)~~
文摘The situation of intercepted alien pests from imported hides in Hebei during 2013-2014 was systematically analyzed. The results showed that 163 batches of alien pests were intercepted, including 2 batches of quarantine weeds and they both were Italian cocklebur. According to the statistics of original countries, the intercepted hides from 21 countries all had alien pests, in which Australia was the largest number of batches and species of alien pests. The analysis results showed that there was severe risk in the imported hides carrying alien pests, thus to effectively prevent the invasion of alien pests to our country, several quarantine measures should be taken in the future, such as enhancing the quarantine inspection in ports, and promoting the personnel ability of interception and species identification of alien pests.
文摘异己手综合征(alien hand syndrome,AHS)是由不同中枢神经损害引起的复杂的临床综合征,主要特点为患侧上肢或手不受意识支配,双手执行动作时存在明显地不协调或拮抗[1]。近年来国内报道较少,加之该病的临床表现复杂多样,症状辨别非常困难,故非常容易漏诊、误诊。现将我院收治的一例AHS患者报道如下。1临床资料患者男性,77岁,右利手,
文摘This thesis,by analyzing the wolf nature,the dog nature and the human nature,and their relations,expounds the author's personal interpretation of the theme of Sherwood Anderson's short story Death in the Woods.The author maintains that Anderson reveals to readers the ugliness of life and the strange beauty of life,condemns the loss of love and the dehumanization of human life in the modern world and implies Anderson's deep concern on the human alienation,an important theme in the 20th century modern literature.