Effects of He-Ne laser irradiation on chronic atrophic gastritis in rats

AIM: To study the effects of He-Ne laser irradiation on experimental chronic atrophic gastritis (CAG) in rats.METHODS: Sixty-three male adult Wistar rats were randomly divided into five groups including normal control group, model control group and three different dosages He-Ne laser groups. The chronic atrophic gastritis (CAG)model in rats was made by pouring medicine which was a kind of mixed liquor including 2% sodium salicylate and 30% alcohol down the throat for 8 wk to stimulate rat gastric mucosa, combining with irregular fasting and compulsive sporting as pathogenic factors; 3.36, 4.80, and 6.24J/cm2doses of He-Ne laser were used, respectively for three different treatment groups, once a day for 20 d. The pH value of diluted gastric acid was determined by acidimeter,the histopathological changes such as the inflammatory degrees in gastric mucosa, the morphology and structure of parietal cells were observed, and the thickness of mucosa was measured by micrometer under optical microscope.RESULTS: In model control group, the secretion of gastric acid was little, pathologic morphological changes in gastric mucosa such as thinner mucous, atrophic glands, notable inflammatory infiltration were found. After 3.36 J/cm2 dose of He-Ne laser treatment for 20 d, the secretion of gastric acid was increased (P＜0.05), the thickness of gastric mucosa was significantly thicker than that in model control group (P＜0.01), the gastric mucosal inflammation cells were decreased (P＜0.05). Morphology, structure and volume of the parietal cells all recuperated or were closed to normal.CONCLUSION: 3.36J/cm2 dose of He-Ne laser has a significant effect on CAG in rats.

The influence of He-Ne laser on scar formation after trabeculectomy in rabbits

AIMTo investigate the influence of He-Ne lasers on scar formation in the filtration canal after trabeculectomy in a rabbit model, as well as to explore the mechanisms for preventing scar formation when using He-Ne lasers in vivo.

The Effects of He-Ne Laser and Enhanced Ultraviolet-B Radiation on Proliferating-Cell Nuclear Antigen in Wheat Seedlings

The level of ultraviolet-B (UV-B) radiation on the Earth’s surface has increased due to depletion of the ozone layer. Here, we explored the effects of continuous wave He-Ne laser irradiation (632 nm, 5 mW·mm-2, 2 min·d-1) on proliferating-cell nuclear antigen (PCNA) damage repair function of wheat seedlings exposed to enhanced UV-B radiation (10.08 kJ&middotm-2·d-1) at the early growth stages. Wheat seedlings were irradiated with enhanced UV-B, He-Ne laser treatment or a combination of the two. We explored the transcripts of PCNA in each treatment group using RT-PCR. In addition, total proteins were extracted from the 7-day-old wheat leaves, analyzed by SDS-PAGE and identified by western blot. The results showed that the transcription of PCNA was weakened following UV-B radiation compared to the control. However, when seedlings were subjected to elevated UV-B-damaging radiation followed by He-Ne laser irradiation, the expression of PCNA was signifi-cantly higher than UV-B radiation alone. These results suggest that He-Ne laser has an active role in repairing the UV-B damaging effects. In order to further investigate the function of PCNA, dynamic arrangements of PCNA in wheat root-tip cells were observed with confocal laser scanning microscopy (CLSM). The PCNA was marked fluorescent dimming and strength weakened in en-hanced UV-B radiation (UV-B) compared with the control group (CK) during processing. It shows that PCNA may be involved in the separation of chromosomes.

Protecting effect of He-Ne laser on winter wheat from UV-B radiation damage by analyzing proteomic changes in leaves

The ultraviolet-B (UV-B) radiation can affect gene expression of plant in growth and development. Winter wheat (Triticum aestivum), as a kind of economic crop cultured in the Northern China, is also damaged by present-day ultraviolet-B (UV-B) radiation. It was reported that He-Ne lasers could alleviate cell damage caused by UV-B radiation in plants. To get the proteomic changes of wheat alleviated by He-Ne lasers, we studied protein expression profiles of winter wheat (Jin Mai NO.79) leaves by running 2-DE (two-dimensional gel electrophoresis), which allowed the identification of some significantly different gel spots. The spots were further verified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry, in which they were confirmed to be winter wheat proteins. The results showed: 1) the proteomic changes between the ultraviolet-B radiation group and normal light group were significantly different on sixth day;2) The expression of some proteins such as catalase, DNA polymerase and reduced glutathione declines at the ultraviolet-B radiation group and increases at the UV-B + He-Ne laser group. This indicates UV-B could regulate genes encoding proteins in Winter wheat leaves and affect the growth of the Winter wheat. He-Ne lasers treatment could significantly alleviate UV-B-induced damage in wheat.

Enhancement of Human Blood Storage Period by Irradiation of Low Level He-Ne Laser

The aim of the present work is to investigate the effect of the He-Ne laser irradiation on the whole human blood (HB) in order to enhance the conditions of conservation. The HB was irradiated by He-Ne laser;(λ = 632 nm, continuous wave, power 30 mW, 2 mm diameter beam spot), electrical properties and complete blood count CBC were measured at three doses (0.0287, 0.0563 and 0.198 J/cm3) to the relevant best exposure dose during storage periods 9, 24, 30, 35 & 50 days. The irradiation process with the selected dose was performed by the exposure of the laser beam on the blood sample flow through narrow tube of cross section area, 0.0831 cm2. Blood dielectric parameters, (electric conductivity, dielectric constant, dielectric loss and dipole moment) and CBC, (red blood cell, white blood cell, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, and mean cell or corpuscular hemoglobin in concentration) were measured. The obtained results were compared with that of the control and showed that the best irradiation exposure dose suitable for increasing the time of blood storage with minimum changes in properties is 0.198 J/cm3 and storage period of about 50 days. The present study revealed that irradiation by He-Ne laser could be considered a good means to improve the conservation conditions of human blood.

The Photopolymerization Kinetics of Acryl amide Sensitized with Toluidine Blue-Alcoholamine Induced by He-Ne Laser

Toluidine blue(TB) is a good sensitizer for the polymerization of acrylnmide(AM) induced by He-Ne laser. With TB as a sensitizer and various alcoholamine as donors, the photoredox of TB and the polymerization kinetics of AM induced by He-Ne laser were investigated. It was found that either for the photoredox of TB or for the polymerization of AM, the activity of alcoholamine increases in the order of EOA<DEOA <TEOA in TB-alcoholamine sensitized system. The overall apparent kinetic equations of AM polymerization at 30℃ have been determined. In addition, the molecular weights under various conditions was measured.

THE PHOTOPOLYMERIZATION KINETICS OF PHOTOSENSITIZED ACRYLAMIDE SYSTEM INDUCED BY He-Ne LASER

The kinetics of the photopolymerization of sensitized acrylamide (AM) system induced by He-Ne laser has been investigated. Using methylene blue (MB)-triethanolamine (TEOA) as the photosensitive system, the photopolymerization followed a nonsteady-state kinetic scheme in the initial period of polymerization (the monomer conversion C%<2%) and then followed a steady-state kinetic scheme (5% >C%>2%). Accirding to the steady-state hypothesis, the mechanism of photopolymerization was proposed. The deduced kinetic equation of the photopolymerization of AM is in good coincidence with the experimental results.

Anomalous Variation of Beat Frequency in a Dual Frequency He-Ne Laser

The beat frequency in a dual frequency He-Ne laser varies while the resonant cavity length is tuned. As to the laser with two longitudinal modes, the variation amplitude is commonly less than 500 kHz, proven by experiments and theories. This study reveals an anomalous variation of the beat frequency when a piece of element is put into the cavity and is aligned with the laser axis. Consequently the variation amplitude couM reach 22 MHz, several dozen times larger than that without the intra-cavity element. This cannot be explained only by laser mode pulling and pushing effects. Some influencing factors are investigated experimentally, including the tilted angle of the element and the distance between its surface and cavity mirror. The qualitative analysis is discussed, which agrees with the experimental results.

The Influence of Diode and He-Ne Lasers on Corn and Wheat Seeds

The paper presents a study on the effects of low intensity laser irradiation on morphological changes in plants sprouted from maize hybrid seeds （two hybrids） and wheat seeds. Pre-sowing laser irradiation treatment on the seeds was done, intervals from 10 s to 15 min （approximately）, by using a diode laser output power of 12 mW at 904 nm wavelength or with He-Ne laser with output power of 50 mW and 632.8 nm wavelength. Before irradiation seeds were divided into groups （wet and dry, and then in subgroups-irradiated or control groups）. We used maize hybrids, Amilacea and Identata and wheat （Triticum aestivum）. The reflection coefficient in visible range was done for maize varieties. Obtained data show the influence of laser beam to better plant growth. Better results are obtained for dry seed irradiation than for wet. In order to investigate the effect of laser beam and in general to clarify a lot of unsolved photo processes related to bioorganisms at macroscopic and microscopic levels, some optical constants of selected plant families were researched. At the same time, the influence of laser beams of common wavelengths to the selected plants was monitored. Morphological processes of plants （seeds and leaves） irradiated under different conditions and plant growing dynamics were contemplated. The definite correlation analyses of obtained results were made, clearly speaking about the influence of small-dose radiation to characteristics （quantitative and other genetic, bio-stimulating effects） of future plant growth.

Efficacy of He-Ne Laser in Combination with Topical Clindamycin in the Treatment of Acne Vulgaris (AV)

Objective: To evaluate the efficacy of He-Ne laser in combination with topical clindamycin in the treatment of AV at 108 Military Central Hospital from Oct 2015 to Aug 2016. Subjects and Methods: 61 patients with AV were divided into 2 groups: Group 1: 31 patients were treated with He-Ne laser in combination with topical Clindamycin, Group 2: 30 patients were only treated with topical clindamycin. Laser HE-NE was applied 2 times/week for 6 weeks. Result: After 3 months of treatment Group 1: very good—51.6%, good—48.4%. Group 2: very good—30%, good—50% and moderate—20%. Conclusion: The combination of He-Ne laser with topical clindamycin shows more rapid clinical improvement compared to topical clindamycin alone in the treatment of mild and moderate AV.

Effect of He-Ne Laser on the Expressions of EGF and EGFR in Gastric Mucosa of Chronic Atrophic Gastritis

To investigate the histological change and the expressions of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in atrophic gastric mucosa in rats so as to appraise the effect of these regulators on the formation of atrophic gastritis, to study the expressions of EGF and EGFR in rats with chronic atrophic gastritis (CAG) after the irradiation of He-Ne laser, and to investigate the relations of He-Ne laser and precancerous lesion or apoptosis. The rats were divided into normal group, model group and laser group. The models of CAG rats were established with enema which was the mixed liquor consisted of sodium salicylate and alcohol, combined with irregular fasting and compulsive sporting as pathogenic factors. He-Ne laser(3.36 J/cm2 ) was used to irradiate CAG rats, once a day for 7 min, 20 days as a course of treatment; the expressions of EGF and EGFR were detected with immunohistochemical method. During the process of antral atrophy, the expressions of EGF and EGFR increased, they were higher in model group than those in normal group (P<0.05). After the irradiation of He-Ne laser, the expressions of EGF and EGFR were obviously lower than those in model group (P<0.05). We draw the conclusion as follows: gastric mucosa of model rat is in a hyper-proliferation status, with high protein expressions of apoptosis suppressor EGF and EGFR. CAG has some correlation with the imbalance between cell proliferation and apoptosis. He-Ne laser (3.36 J/cm2 ) can reduce the expressions of EGF and EGFR, which is an effective physiostimulator to stimulate the gastric mucosa of rat. The appropriate secretions of EGF and EGFR are propitious to the repair and regeneration for the gastric mucosa tissues, thus preventing CAG to canceration.

DUAL-FREQUENCY LASER USING ANISOTROPIC BIREFLECTANCE FILM(1.2-6.0 MHz)AND ITS POLARIZATION CHARACTER

The appropriate bireflectance film series are selected to get thebeat-frequency of dual-frequency laser using anisotropic bireflectance film (DLABF) from 1.2 MHz to6 MHz. The maximum measurement velocity of the interferometer utilizing DLABF can be up to 1.8 m/s.Generally the outputs of the DLABF in a transverse magnetic field are two orthogonalmicro-elliptical polarized components when DLABF can emit dual-frequency laser. When the laser tubeis spun in the magnetic field, the ellipticities of these two components are also changed. In somecertain relative angles, the outputs the DLABF are two almost ideally orthogonal linear polarizedcomponents. The frequency stabilization of DLABF is also discussed. The maximum variation of thebeat-frequency of the stabilized DLABF can be 0.15 kHz within 46 h. As no quarter wave plate (QWP)needed, DLABFs have better thermal stability than longitudinal Zeeman lasers.

Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA

Using agarose gel electrophoresis and T4-endo-deoxyribonuclease-V, which is a kind of restriction endonu-clease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5mW·mm-2) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-Y had been stimulated in the wheat cells.

Effects of Chinese Herbal Medicine Combined with He-Ne Laser on Lipoperoxide and Superoxide Dismutase in Chloasma Patients

Objective: To observe the effects of Chinese decoction and ligustrazin hydrochloride injection combined with He-Ne laser on lipoperoxide (LPO) and Superoxide dismutase (SOD) in patients with chloasma. Methods: 90 cases of chloasma were randomly divided into the following two groups: a treatment group (of 54 cases) treated by a self-prepared prescription for toning the kidneys and relieving the depressed liver to remove blood stasis, ligustrazin hydrochloricde injection and He-Ne laser therapy, and a control group (of 36 cases) treated with oral administration of Vitamin E and Vitamin C plus external application of 20% Azelaic acid cream. Results: The total effective rate in the treatment group was 79.6%, which was significantly higher than that of the control group (P<0.05). After treatment, the LPO level in the treatment group was significantly lowered (P<0.01), and the SOD level was significantly elevated (P<0.05). Conclusion: The therapeutic methods adopted in the treatment group may show the action of antioxidation, providing good clinical effects for treating chloasma.

Large-range alignment-free distributed-cavity laser based on an improved multi-lens retrorefleetor

A multi-lens retroreflector with field curvature compensation was designed and used in an alignment-free distributed-cavity laser with a long working distance for resonant beam charging applications.The multi-lens design,which makes use of off-the-shelf components,also allows a large field of view[FoV]without requirement of large element aperture.By implementing this design,an end-pumped 1063 nm Nd:GdVO4 laser could deliver over 5 W continuous-wave output power over a large range of working distances(1-5 m)and with ±30°receiver FoV under an incident diode pump power of 16.6 W.The output power fluctuation was less than 10%when moving and tilting the receiver over such a large range,without requiring any realignment of the cavity.

Extra-short He-Ne lasers based on mode split

An extra-short He-Ne laser (shorter than 100mm) does not work well because there is likely no longitudinal mode in the lasing bandwidth sometimes and the output power rises and falls deeply from time to time.A method to solve this problem has been presented and investigated.A piece of quartz crystal plate appropriately designed is used as a cavity mirror of a He-Ne laser,which has a longitudinal mode spacing larger than the lasing bandwidth to make the equal-spacing mode split.In other words,the number of longitudinal modes is doubled and the actual mode spacing is reduced to the half of that without mode split.Therefore,there is always at least one mode operating in the lasing bandwidth and the power is stable at any room temperature.Such a laser with 85mm cavity length and 0.24mW output power has been made.

He-Ne laser acupuncture for 30 cases of unilateral nervous tinnitus

Objective： To observe the clinical effects of the unilateral nervous tinnitus treated by He-Ne laser acupuncture.Methods： A total of 30 patients of unilateral nervous tinnitus were treated with He-Ne laser acupuncture.The disposable filiform needles of 0.40 mm × 25 mm were used. Ermén（耳门 TE 21）, Tīnggōng（听宫 SI 19）,Tinghui（听会 GB 2）, Yìfēng（翳风 TE 17）,Shuàigǔ（率谷 GB 8） and Zhōngzhǔ（中渚 TE 3） were adopted on the affected side. The input and output ends of the fiber-optic catheter of the laser instrument were connected at a pair of acupoints（TE 21 connect to SI 19, GB 2 connect to TE 17, GB 8 connect to TE 3）.Each pair of acupoints was stimulated with He-Ne laser irradiation for 10 min, 8-10 mW in the output power and 6328 A in the wavelength. The needles were retained for 30 min totally. The treatment was given once a day, 5 times a week, continuously for 2 weeks.Results： Of 30 cases, 19 cases were cured clinically, accounting for 63.3%（19/30）; 7 cases effective remarkably, accounting for 23.3%（7/30） and 4 cases failed, accounting for 13.3%（4/30）.Conclusion： He-Ne laser acupuncture achieves the satisfactory effects on treating nervous tinnitus and it is easily accepted by the patients. Hence, this therapy deserves to be promoted in clinical application.

Cavity tuning characteristics of orthogonally polarized dual-frequency He-Ne laser at 1.15 μm

The cavity tuning characteristics of orthogonally polarized dual-frequency HeiNe laser at 1.15 μm are presented. Vectorial-extension model based on semi-classical laser theory reveals that cavity tuning characteristics are related to beat frequency, relative excitation, and type of Ne isotope. Distortions of cavity tuning curves become moderate with the increase of beat frequency because of the weakening of the cross- saturation effect. Distortions are enhanced with the increase of relative excitation because of the combined action of the self-saturation and cross-saturation effects. By adopting dual-isotope Ne instead of monoisotoplie Ne, distortions are reduced because of the misalignment between peaks of the self-saturation and net gain coefficients. The theoretical calculations are in good agreement with the corresponding experimental results.

RELATION OF MOXIBUSTION THERAPY TO CUTANEOUS RECEPTORS

In this paper, authors have observed the effect of suspended moxibustion and He-Ne laser moxibustion on the discharge of 125 units which selected from 12 types of cutaneous receptors in rabbits. The temperature of suspended moxibustion was limited at 47 - 48℃. This moxibustion method can induce the excitatory reaction of every unit observed of thermoreceptor, Aδ and C poly modal receptors; while for the down hair receptor, Merkel’s tactile disk, Ruffini’s corpuscle and C mechanonociceptor, it can produce an inhibition on part units of the receptors mentioned above which have spontaneous discharge; and the other 5 types of receptors all have no response to it. The results show that the action of suspended moxibustion on the acupoint has a close relation to the thermorecep tor or/and the polymodal receptor. The power of laser moxibustion is 4 mW, radiating one minute, all 125 units of 12 types of cutaneous receptors have no change of discharge to this radiation, suggesting that the curative effect of the radiation with laser of the low power seems not to have internal relations each other.

氦—氖激光器配气方法探讨

氦—氖激光管制作中,将原来先充氦的作法改为先充氖,并对配气公式进行了完整地推导,从而改变了实验中氦气耗量过大和氖耗量太少的悬殊比例。