A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex ...A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex Havil(UR)were chosen to show the advantage of this method.The sample enrichment and separation were展开更多
A pair of indole alkaloid enantiomers with a novel bisindolylacetamide skeleton, insatindibisindolamides A and B（1a and 1b）, was isolated from an aqueous extract of Isatis indigotica roots. The enantiomers were sepa...A pair of indole alkaloid enantiomers with a novel bisindolylacetamide skeleton, insatindibisindolamides A and B（1a and 1b）, was isolated from an aqueous extract of Isatis indigotica roots. The enantiomers were separated by chiral HPLC. Their structures and absolute configurations were elucidated by extensive spectroscopic analysis, including 2D NMR, X-ray crystallography, and electronic CD（ECD） calculation. The proposed biosynthetic pathway and preliminary investigations of the biological activity of compounds 1a and 1b are also discussed.展开更多
基金supported by the National Science and Technology Major Project for Major Drug Development(No.2013ZX09508104)the Traditional Chinese Medicine Industry Research Special Project(No.201307002)the National Science&Technology Major Project Key New Drug Creation and Manufacturing program(No.2011ZX09307002-03)of the People's Republic of China
文摘A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex Havil(UR)were chosen to show the advantage of this method.The sample enrichment and separation were
基金Financial support from the National Natural Science Foundation of China(NNSFCNos.81373287 and 30825044)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT,No.IRT1007)the National Science and Technology Project of China(Nos.2012ZX09301002-002 and 2011ZX0 9307-002-01)
文摘A pair of indole alkaloid enantiomers with a novel bisindolylacetamide skeleton, insatindibisindolamides A and B（1a and 1b）, was isolated from an aqueous extract of Isatis indigotica roots. The enantiomers were separated by chiral HPLC. Their structures and absolute configurations were elucidated by extensive spectroscopic analysis, including 2D NMR, X-ray crystallography, and electronic CD（ECD） calculation. The proposed biosynthetic pathway and preliminary investigations of the biological activity of compounds 1a and 1b are also discussed.
