Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection ...Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection of slgE have been developed. The objective of this study is the comparison of the ALLERG-O-LIQ with the ImmunoCAW System using seven inhalant and four food allergens. Methods： Sera from patients were collected and tested for slgE to inhalant（dl, d2, d5, i6, el, e5 and m3） and food allergens（fl, f2, f24, f24） by ALLERG-O-LIQ（Dr. Fooke Laboratorien GmbH） and by ImmunoCAP System（Phadia）. Further, samples were also tested for total IgE in both systems. Results： Prevalence of positive test results varied between 0/20（f24） and 11/20（e5） for ALLERG-O-LIQ and between 3/18（f23） and ll/20（dl/d5） for ImmunoCAP. The qualitative agreement between both methods was found between 75%（f24） and 100%（d2） depending on the allergen. Overall qualitative agreement for inhalant（n = 140）, food（n = 78） and all allergens（n = 218） tested was 92.1%（kappa = 0.84）, 83.3%（kappa = 0.58）, 89.0%（kappa = 0.77）, respectively. Sensitivity, specificity, positive predictive value （PPV）, negative predictive value（NPV） and diagnostic efficiency（DE） were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%（inhalant allergens）, 62.5%, 92.6%, 78.9%, 84.7%, 83.3%（food allergens） and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%（all allergens）. Conclusion： Good to excellent qualitative agreement between ALLERG-O-LIQ and ImmunoCAP for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-LIQ System represents a reliable test for the detection of specific and total IgE.展开更多
Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate ...Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.展开更多
Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigati...Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigating these diseases is increasing and becoming expensively high. There are many ways to explore allergenic antibodies to assess the presence the amount of specific IgE as: Skin test (Prick), Specific IgE (ELISA), RAST Sp.IgE and Elimination Challenge method. Skin test produces pain, local and anaphylactic reaction and patient discomfort, other procedure with highly cost to patient. So, we have built up an evidence to use it in our allergy and immunology clinic has suitable cost for all patients based about for the other tests but it was very simple, accurate, cheap and does not produce any problems to patients. Our test depends on ELISA technique, it measures the quantitative amount of the following different allergens: As regard food allergens: Milk, Eggs, Banana, Maize, Fish, Chocolate, Wheat, Nuts, Strawberry, Shrimps, Spices, with drug as Aspirin. As regard inhalants as: House dust, Mite, Mixed Pollens, Mixed Moulds, Hay dust, Wool, Latex and Cat Hair. 150 allergic patient results of our test compared with specific IgE national kits (ELISA), Sp.IgE (RAST), Skin test and elimination challenge test. The statistical evaluation results as regard senistivity respectively: 88.9%, 89.6%, 91.2%, 71.4%, 93.1%. As regard specificity the results: 93.1%, 94.7%, 95.3%, 65.5%, 91.6%, the latter results showed that our test was in line with all tests. Our test was probably in food, drug and inhalant allergens, it can be also noted that the cheapest and most commercial technique was used, so as to be available immediately in standard laboratory and its reagents, plate and other requirements were prepared locally in our laboratory. Now it was applying successfully in allergy and immunology unit Ain Shams university hospitals in Egypt.展开更多
许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空...许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空气中的NO_(2)和O_(3)硝基化,从而影响线性表位与IgE的结合能力.本实验研究了Der p 2的线性表位及其硝基化产物与IgE的结合能力.研究发现,Der p 2的两条表位多肽可以有效地结合IgE,硝基化表位多肽的IgE结合能力显著高于未硝基化的表位多肽,且不同位点的硝基化对于IgE结合能力的增强程度也不同.结果表明,硝基化能够位点特异性地增强Der p 2的致敏性.展开更多
目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1...目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1。使用人源IgE免疫新疆单峰驼提取淋巴细胞中的RNA建立噬菌体展示纳米抗体库,分析库容、多样性和插入率,通过筛选和鉴定获得抗人IgE纳米抗体。使用重组过敏原偶联磁微粒和吖啶酯标记纳米抗体,建立磁微粒化学法猫皮屑特异性IgE抗体检测方法。结果:噬菌体展示库的库容为1.88×108 cfu/ml,插入率为93.6%,纳米抗体纯度>95%。检测方法的线性范围为0.1~100 U/ml,与ImmunoCAP检测系统临床对比有较好的一致性。结论:成功建立了基于纳米抗体的猫皮屑特异性IgE抗体超敏检测方法,为猫过敏性疾病辅助诊断提供了技术基础。展开更多
基金the Science and Technique Fund(2005B30601014)the Industry Technology and Development Fund(2007B030701007)
文摘Objective： Beside patient＇ s history and skin prick testing（SPT） the detection of specific IgE（slgE） represents an important tool of allergy diagnostics. In recent years different technologies for the detection of slgE have been developed. The objective of this study is the comparison of the ALLERG-O-LIQ with the ImmunoCAW System using seven inhalant and four food allergens. Methods： Sera from patients were collected and tested for slgE to inhalant（dl, d2, d5, i6, el, e5 and m3） and food allergens（fl, f2, f24, f24） by ALLERG-O-LIQ（Dr. Fooke Laboratorien GmbH） and by ImmunoCAP System（Phadia）. Further, samples were also tested for total IgE in both systems. Results： Prevalence of positive test results varied between 0/20（f24） and 11/20（e5） for ALLERG-O-LIQ and between 3/18（f23） and ll/20（dl/d5） for ImmunoCAP. The qualitative agreement between both methods was found between 75%（f24） and 100%（d2） depending on the allergen. Overall qualitative agreement for inhalant（n = 140）, food（n = 78） and all allergens（n = 218） tested was 92.1%（kappa = 0.84）, 83.3%（kappa = 0.58）, 89.0%（kappa = 0.77）, respectively. Sensitivity, specificity, positive predictive value （PPV）, negative predictive value（NPV） and diagnostic efficiency（DE） were found at 88.2%, 95.8%, 95.2%, 89.6%, 92.1%（inhalant allergens）, 62.5%, 92.6%, 78.9%, 84.7%, 83.3%（food allergens） and 81.5%, 94.4%, 91.5%, 87.5%, 89.0%（all allergens）. Conclusion： Good to excellent qualitative agreement between ALLERG-O-LIQ and ImmunoCAP for the detection of specific and total IgE could be observed. The degree of agreement depended on the allergen and was higher in the group of inhalant allergens. The ALLERG-O-LIQ System represents a reliable test for the detection of specific and total IgE.
文摘Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.
文摘Allergy is a serious health problem throughout the world, affecting people of all ages. Allergic diseases such as asthma, rhinitis and atopic dermatitis are becoming an epidemic in our country, the cost of investigating these diseases is increasing and becoming expensively high. There are many ways to explore allergenic antibodies to assess the presence the amount of specific IgE as: Skin test (Prick), Specific IgE (ELISA), RAST Sp.IgE and Elimination Challenge method. Skin test produces pain, local and anaphylactic reaction and patient discomfort, other procedure with highly cost to patient. So, we have built up an evidence to use it in our allergy and immunology clinic has suitable cost for all patients based about for the other tests but it was very simple, accurate, cheap and does not produce any problems to patients. Our test depends on ELISA technique, it measures the quantitative amount of the following different allergens: As regard food allergens: Milk, Eggs, Banana, Maize, Fish, Chocolate, Wheat, Nuts, Strawberry, Shrimps, Spices, with drug as Aspirin. As regard inhalants as: House dust, Mite, Mixed Pollens, Mixed Moulds, Hay dust, Wool, Latex and Cat Hair. 150 allergic patient results of our test compared with specific IgE national kits (ELISA), Sp.IgE (RAST), Skin test and elimination challenge test. The statistical evaluation results as regard senistivity respectively: 88.9%, 89.6%, 91.2%, 71.4%, 93.1%. As regard specificity the results: 93.1%, 94.7%, 95.3%, 65.5%, 91.6%, the latter results showed that our test was in line with all tests. Our test was probably in food, drug and inhalant allergens, it can be also noted that the cheapest and most commercial technique was used, so as to be available immediately in standard laboratory and its reagents, plate and other requirements were prepared locally in our laboratory. Now it was applying successfully in allergy and immunology unit Ain Shams university hospitals in Egypt.
文摘许多过敏原可以介导Ⅰ型超敏反应,通过与IgE特异性结合,引起过敏症状.过敏原与细胞表面的特异性IgE结合的部分叫做表位,其与IgE的结合能力可以表征过敏原致敏性的强弱.Der p 2是一种重要的屋尘螨过敏原,其线性表位中含有的酪氨酸可被空气中的NO_(2)和O_(3)硝基化,从而影响线性表位与IgE的结合能力.本实验研究了Der p 2的线性表位及其硝基化产物与IgE的结合能力.研究发现,Der p 2的两条表位多肽可以有效地结合IgE,硝基化表位多肽的IgE结合能力显著高于未硝基化的表位多肽,且不同位点的硝基化对于IgE结合能力的增强程度也不同.结果表明,硝基化能够位点特异性地增强Der p 2的致敏性.
文摘目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1。使用人源IgE免疫新疆单峰驼提取淋巴细胞中的RNA建立噬菌体展示纳米抗体库,分析库容、多样性和插入率,通过筛选和鉴定获得抗人IgE纳米抗体。使用重组过敏原偶联磁微粒和吖啶酯标记纳米抗体,建立磁微粒化学法猫皮屑特异性IgE抗体检测方法。结果:噬菌体展示库的库容为1.88×108 cfu/ml,插入率为93.6%,纳米抗体纯度>95%。检测方法的线性范围为0.1~100 U/ml,与ImmunoCAP检测系统临床对比有较好的一致性。结论:成功建立了基于纳米抗体的猫皮屑特异性IgE抗体超敏检测方法,为猫过敏性疾病辅助诊断提供了技术基础。
