一株拮抗链格孢(Alternaria spp.)抗生素产生菌研究

从冷冻菌种甘油管中分离到一株生长力极强的污染细菌,该细菌能产生抑制链格孢真菌的活性物质,在研究抑菌活性物质的过程中,对这株细菌进行分类鉴定.通过传统的表型及生理生化方法,将该菌定位到芽孢杆菌属(Bacillus),与枯草芽孢杆菌(B.subtilis)的相似性最大,最终确定这株菌为B.subtilis中的一个株型,即Bacillus subtilis subsp.subtilis str.168.以链格孢属真菌(包括原有的和从空气、小麦病斑分离的)为受试菌,对此株细菌产生抑菌物质的时间进行跟踪,当该菌进入稳定生长期后开始产生抑菌物质,72 h时积累达到最大值.对抑菌物质进行过滤、加热、调节pH值等多种处理,初步确定抑菌物质的特性为外泌型、脂溶性,可透过孔径0.22μm的微孔滤膜,可耐受100℃5 m in,80℃10 m in的高温,微碱性条件不影响其生物活性.

蛋白激发子产生菌链格孢(Alternaria spp.)JH505菌株分子系统学研究

在传统真菌形态学分类研究的基础上,运用遗传学、分子生物学研究手段,结合生物信息学方法对具有诱导植物增产抗病的蛋白激发子产生菌链格孢(Alternaria spp.)JH505菌株进行了分子系统学研究。克隆了该菌株长度为1 267bp的rDNA序列。结果表明,rDNA包含部分18S rRNA、28S rRNA序列及完整的5.8S rRNA、转录外间隔区(ITS1、ITS2)rRNA序列,与A.tenuissima、A.cinerariae、A.dianthi、A.brassicae、A.zinniae相应的rDNA分别具有100%、98%、97%、97%和96%的相似性。结合菌落形态、孢子特征以及rDNA分析,鉴定该菌株为细极链格孢菌(A.tenuissima)。

基于改进YOLOv3-SPP算法的道路车辆检测

针对在城市道路场景下视觉检测车辆时,车辆密集和远处车辆呈现小尺度,导致出现检测精度低或者漏检的问题,提出了一种基于改进的YOLOv3-SPP算法,对激活函数进行优化,以DIOU-NMS Loss作为边界框损失函数,增强网络的表达能力。为提高所提算法对小目标和遮挡目标的特征提取能力,引入空洞卷积模块,增大目标的感受野。实验结果表明,所提算法在检测车辆目标时m AP提高了1.79%,也有效减少了在检测紧密车辆目标时出现的漏检现象。

FOSB、SPP1基因表达对经肝动脉介入化疗栓塞术治疗的中晚期肝癌生存期预测价值

目的探讨骨肉瘤原癌基凶同源物B(FOSB)、分泌性磷蛋白1(SPP 1)基因表达对经肝动脉介入化疗栓塞术治疗的中晚期肝癌患者术后生存期的预测价值。方法回顾性分析2016年1月—2017年10月在西安医学院第一附属医院进行诊治的中晚期肝癌患者115例作为研究对象,根据FOSB、SPP 1基因表达水平分为FOSB高表达组(n=52)、FOSB低表达组(n=63)及SPP 1高表达组(n=89)、SPP 1低表达组(n=26)。同时选取115例健康体检者为健康对照组。分析FOSB、SPP 1表达与中晚期肝癌患者临床病理特征的关系。对纳入研究的患者进行为期60个月的随访,Logistics风险回归模型分析影响患者生存期的危险因素。Kaplan-Meier生存曲线分析FOSB、SPP 1表达水平与患者生存预后的关系。结果肝癌患者外周血单个核细胞中FOSB mRNA表达水平明显低于健康对照组,SPP 1 mRNA表达水平明显高于健康对照组,差异具有统计学意义(P<0.05)。FOSB、SPP 1表达在中晚期肝癌患者肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期方面比较差异具有统计学意义(P<0.05)。单因素分析结果显示,存活组和死亡组在肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期、FOSB、SPP 1表达方面比较,差异均具有统计学意义(P<0.05)。肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期、FOSB低表达、SPP 1高表达均为中晚期肝癌患者生存期的独立危险因素(P<0.05)。随访时间60个月,患者生存率为17.39%(20/115),FOSB高表达组中位生存时间为39.7个月,明显高于FOSB低表达组的19.3个月(P<0.05);SPP 1低表达组中位生存时间为40个月,明显高于SPP 1高表达组的18个月(P<0.05)。结论FOSB在中晚期肝癌患者中表达明显下调,SPP 1表达上调,其对预测中晚期肝癌患者肝动脉介入化疗栓塞术治疗生存期具有一定的价值。FOSB、SPP 1有望成为评估介入术治疗患者预后的潜在指标,可协同肿瘤大小、Child-Pugh分级、淋巴转移、BCLC分期等临床指标预测或评估肝癌患者术后生存情况。

无花果内生菌Alternaria sp.QDFB-2菌丝体化学成分的研究

为研究无花果枝条内生真菌Alternaria sp.QDFB-2菌丝体的化学成分,采用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相柱层析和高效液相色谱等方法对Alternaria sp.QDFB-2菌丝体粗浸膏进行分离纯化,利用核磁共振谱(NMR)和质谱(MS)等现代波谱技术、结合文献比对方法确定化合物的结构;采用MTT法进行化合物的体外细胞毒活性评价。结果表明:从Alternaria sp.QDFB-2菌丝体粗浸膏中分离纯化得到7种化合物:alternariol(1)、tentoxin(2)、dihydrotentoxin(3)、cyclo(Gly-Pro)(4)、cyclo(4-hydroxy-Pro-Pro)(5)、cyclo(S-Leu-S-N-methyl-Phe)(6)和uridine(7)。体外细胞毒活性评价显示,化合物1和7对SMMC-7721细胞具有中等增殖抑制活性,其IC50值分别为50.47μg·mL^(-1)(195.62μmol·L^(-1))和42.50μg·mL^(-1)(174.18μmol·L^(-1));化合物1对A549细胞也具有中等的增殖抑制活性,浓度为100μg·mL^(-1)时抑制率为52.12%;阳性对照顺铂的IC50值分别为3.46μg·mL^(-1)(11.53μmol·L^(-1))和5.12μg·mL^(-1)(17.06μmol·L^(-1))。Alternaria sp.QDFB-2菌丝体的化学成分丰富,值得深入研究。

响应面法研究链格孢菌Alternaria ochroleuca的产毒条件

比较了不同链格孢菌(Alternaria ochroleuca、Alternaria alternata和Alternaria tenuissima)的产毒能力,并通过单因素实验和响应面试验考察了不同的培养温度、培养时间和培养基初始pH对A.ochroleuca产主要链格孢霉毒素链格孢酚(AOH)、链格孢甲基醚(AME)和细交链格孢菌酮酸(TeA)3种毒素的影响。结果表明:3种毒素的产量均随培养温度、培养时间和培养基初始pH的增加呈先增加后逐渐减少的趋势,培养温度26.8℃、培养时间11.4 d、培养基初始pH 4.7是TeA的最佳产生条件,最大产量为4841.3 mg∕kg;当培养温度为25.9℃、培养时间为7.8 d、培养基初始pH为6.4时,AOH和AME的产量最高,分别达2096.1 mg∕kg和152.8 mg∕kg。培养温度和培养基初始pH对TeA的产生具有显著的交互作用,而培养温度和培养时间对AOH和AME的产生具有显著的交互作用。本研究明确了链格孢菌A.ochroleuca产生链格孢霉毒素的主要影响因素,对控制链格孢霉毒素污染、保障食品安全具有重要意义。

SPP1和PD-L1在子宫内膜癌组织中的表达及其预后价值

目的:分析分泌型磷蛋白1(secreted phosphoprotein 1,SPP1)和程序性死亡蛋白配体1(programmed cell death-ligand 1,PD-L1)在子宫内膜癌(endometrial carcinoma,EC)组织中的表达水平及与预后的关系。方法:通过免疫组织化学方法分析SPP1和PD-L1在84例EC组织和61例正常子宫内膜组织中的表达情况,分析SPP1和PD-L1表达与临床病理特征及预后的关系,利用UALCAN分析工具(https://ualcan.path.uab.edu/)分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库中EC组织SPP1和PD-L1 mRNA的表达及与预后的关系。结果:EC组织SPP1阳性表达率高于正常子宫内膜组织(71.43%vs 36.07%,P=0.001),PD-L1阳性表达率低于正常子宫内膜组织(25.00%vs 42.62%,P=0.025);EC组织中,SPP1阳性表达率与组织学分级相关(P<0.05),PD-L1阳性表达率与淋巴结转移相关(P<0.05),SPP1与PD-L1表达呈正相关(列联系数r=0.237,P=0.026);EC组织中,不同SPP1和PD-L1表达患者总生存期无差异(均P>0.05)。TCGA数据库中生物信息学分析显示EC中SPP1 mRNA表达水平显著高于正常子宫内膜组织(P<0.01),PD-L1 mRNA表达水平与正常子宫内膜组织比较,差异无统计学意义(P=0.057);EC组织SPP1 mRNA和PD-L1 mRNA表达水平呈正相关(r=0.350,P<0.05);不同SPP1和PD-L1表达水平患者总生存期无差异(均P>0.05)。结论:SPP1在EC组织中的表达与PD-L1呈正相关,可能共同参与EC免疫微环境调控,SPP1和PD-L1均不是EC独立预后因子。

Genetic Diversity of Jute Mallow (Corchorus spp.) Accessions Based on ISSR Markers

Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.

Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of apple.To understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by RNA-Seq.At each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple leaves.Analysis of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP infection.RLP,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP development.However,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal transduction.Further analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP infection.Transient expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP resistance.Collectively,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

Prevalence and Antibiotic Resistance of Urinary Tract Pathogens, with Molecular Identification of Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp., Using Multiplex Real-Time PCR

Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.

Effect of dissolved organic nitrogen on the bloom of Prorocentrum donghaiense and Karenia spp. in the East China Sea coastal waters

Understan ding the mechanism of harmful algal bloom formation is vital for effectively preventing algal bloom outbreaks in coastal environments.Karenia spp.blooms in the East China Sea show a significant correlation with nutrient regimes.However,the impact of key components of nutrients,especially dissolved organic nitrogen(DON),on the blooms of Karenia spp.is not clear.Quantitative research is still lacking.In this study,the cruise observations,field mesocosm-flask culture experiments,and a multinitrogen-tri-phytoplankton-detritus model(NTPD) are combined to reveal the quantitative influence of nutrient regimes on the shift of Prorocentrum donghaiense and Karenia spp.in the East China Sea.It has a synchronism rhythm of diatom-P.donghaienseKarenia spp.-diatom loop in the field culture experiment,which is consistent with the results of the cruise observation.The results showed that the processes of terrigenous DON(TeDON) and dissolved inorganic nitrogen(DIN:NO_(3)^(-)-N,NH_(4)^(+)-N) absorption promoted P.donghaiense to become the dominant algae in the community;whereas the processes of DON from P.donghaiense absorption promoted Karenia spp.to become the dominant algae in ambient DIN exhaustion.In addition,the three-dimensional fluorescence components of humus C,tyrosine and fulvic acid can indicate the processes of growth and extinction of P.donghaiense and Karenia spp.,respectively.This study infers that P.donghaiense and Karenia spp.regime shift mechanism associated with the nutrient regime in coastal waters,which provides a scientific basis for the environmental management of coastal eco system health.

Strawberry (Fragaria spp.): Cultivation, Production, Consumption, and Marketing in Cameroon

Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms of Fragaria spp. from January 2021 to February 2022. The plant was introduced in Cameroon in 2018. There are 13 varieties of Fragaria spp. currently cultivated. Among these 13 varieties, eleven are hybrids of Fragaria x ananassa (“Amiga”, “Amine”, “Camarosa”, “Chandler”, “Charlotte”, “Elsanta”, “Gariguette”, “Madame Moutot”, “Ostara”, “Ruby gem” and “San Andreas”), and two of the hybrids of Fragaria vesca (“Maestro” and “Mara des bois”). The cropping system, irrigation system, and type of fertilizers applied differ from one strawberry farm to another. Biofertilizers (such as mycorrhizal), inorganic and organic fertilizers are actually used to improve production. The potential annual production of strawberries from January 2021 to February 2022, estimated based on the survey data, was 21.216 tons for all growers. Among these eight production farms, the Lolodorf BIO Farm presents 6000 kg (six tons) of strawberries and 100,000 stolons (seedlings) produced, from seven varieties of Fragaria spp. cultivated, with 6 varieties which are hybrids variety Fragaria x ananassa (“Amiga”, “Amine”, “Chandler”, “Gariguette”, “Madame Moutot”, and “Ruby gem”), and one which is a hybrid of Fragaria vesca (“Mara des bois”). Certain diseases were also observed and recorded depending on the growing areas.

Botulinum toxin type A-targeted SPP1 contributes to neuropathic pain by the activation of microglia pyroptosis

BACKGROUND Neuropathic pain(NP)is the primary symptom of various neurological condi-tions.Patients with NP often experience mood disorders,particularly depression and anxiety,that can severely affect their normal lives.Microglial cells are as-sociated with NP.Excessive inflammatory responses,especially the secretion of large amounts of pro-inflammatory cytokines,ultimately lead to neuroinflam-mation.Microglial pyroptosis is a newly discovered form of inflammatory cell death associated with immune responses and inflammation-related diseases of the central nervous system.METHODS Two models,an in vitro lipopolysaccharide(LPS)-stimulated microglial cell model and a selective nerve injury model using BTX-A and SPP1 knockdown treatments,were used.Key proteins in the pyroptosis signaling pathway,NLRP3-GSDMD,were assessed using western blotting,real-time quantitative polymerase chain reaction,and immunofluorescence.Inflammatory factors[interleukin(IL)-6,IL-1β,and tumor necrosis factor(TNF)-α]were assessed using enzyme-linked immuno-sorbent assay.We also evaluated microglial cell proliferation and apoptosis.Furthermore,we measured pain sensation by assessing the delayed hind paw withdrawal latency using thermal stimulation.RESULTS The expression levels of ACS and GSDMD-N and the mRNA expression of TNF-α,IL-6,and IL-1βwere enhanced in LPS-treated microglia.Furthermore,SPP1 expression was also induced in LPS-treated microglia.Notably,BTX-A inhibited SPP1 mRNA and protein expression in the LPS-treated microglia.Additionally,depletion of SPP1 or BTX-A inhibited cell viability and induced apoptosis in LPS-treated microglia,whereas co-treatment with BTX-A enhanced the effect of SPP1 short hairpin(sh)RNA in LPS-treated microglia.Finally,SPP1 depletion or BTX-A treatment reduced the levels of GSDMD-N,NLPRP3,and ASC and suppressed the production of inflammatory factors.CONCLUSION Notably,BTX-A therapy and SPP1 shRNA enhance microglial proliferation and apoptosis and inhibit microglial death.It improves pain perception and inhibits microglial activation in rats with selective nerve pain.

甘肃省陇南市核桃枝枯病菌Alternaria alternate的生物学特性

以核桃枝枯病菌(Alternaria alternate)为对象,研究温度、碳源、氮源、光照、培养基和pH等对菌丝生长、产孢量和孢子萌发的影响。结果表明,pH 7和全黑暗有利于核桃枝枯病菌的菌丝生长、产孢和孢子萌发;25℃有利于菌丝生长,25~30℃有利于产孢,20~30℃有利于孢子萌发;最适生长的碳源为可溶性淀粉,氮源为酵母浸粉、KNO_(3)和蛋白胨,最适产孢的碳源和氮源分别为山梨醇和KNO_(3),最适孢子萌发的碳源和氮源分别为乳糖和NH_(4)NO_(3);PSA、OA和PDA培养基适合菌丝生长,LB培养基适合产孢,OA培养基适合孢子萌发。该研究结果为明确核桃枝枯病的发生规律提供理论依据,也为综合防治措施的制定提供参考。

Multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

基于SPPs的二维多凹槽结构MIM波导透射特性的研究

不规则结构对波导的传播特性有着很大的影响,但对于MIM(Metal-Insulator-Metal,金属-介质-金属型)波导双边矩形槽并添加多凹槽结构对传播特性的影响方面的研究尚少。在研究内嵌凹槽位置对MIM波导透射特性影响的基础上,提出了在双矩形波导模型基础的内部添加多凹槽结构来影响波导的透射特性,并对波长与透射的关系及不同凹槽位置、不同凹槽数目的电场模图进行了对比分析。在凹槽位置改变时,Fano共振峰位置发生了移动,在小波长区域共振峰的数目也有所改变。随着上凹槽位置的增大,最大透射峰位置发生了蓝移,透射强度也有一定的增强;增加凹槽对数,得到波长与透射特性的关系曲线发生了改变,随着凹槽对数的增加,Fano共振峰的位置发生了一定的红移,且在小波长600~1 000 nm区域,Fano共振峰数目及透射峰值均发生了变化。这些研究结果可为预测金属缝隙波导制备过程中小尺度、多凹槽局部缺陷结构对其传播特性的影响提供理论依据。

差分码偏差产品对BDS-3多频SPP影响分析

为分析不同差分码偏差(differential code bias,DCB)产品对BDS-3多频伪距单点定位(single point positioning,SPP)的影响,基于IGS跟踪站实测数据,对比分析CAS产品、DLR产品、GBM产品解算B1C/B2a、B1I/B3I、B1C/B2a/B3I、B1I/B2a/B3I伪距单点定位精度的影响。试验结果表明:采用CAS产品解算BDS-3双频和三频伪距单点定位精度较优;采用DLR产品解算B1I/B3I和B1I/B2a/B3I伪距单点定位精度较优,但低于CAS产品;采用DLR产品和GBM产品解算B1C/B2a和B1C/B2a/B3I伪距单点定位精度较差,E、N、U 3个方向精度均低于7 m;无论采用哪种产品,三频伪距单点定位精度均优于双频伪距单点定位精度,且提升量较优。

SPP1、OLR1在宫颈癌的表达及临床意义

观察SPP1(分泌型磷酸蛋白1)、OLR1(氧化低密度脂蛋白受体1)在正常宫颈及宫颈癌中的表达情况,探讨二者与宫颈癌相关临床病理资料及预后的关系。方法 选取2021年01月至2023年01月就诊于包头市肿瘤医院的48例宫颈癌患者为病例组,获取同一时间就诊于该院的48例正常宫颈组织为对照组。应用免疫组化法检测SPP1、OLR1在上述两组的表达情况,初步探讨SPP1和OLR1与宫颈癌患者的关系及二者之间的相关性;采用生物信息学分析对比宫颈癌及正常宫颈中SPP1、OLR1的表达,并分析其与宫颈癌患者生存预后的关系。结果 癌组织中SPP1、OLR1阳性表达率高于正常组织(P<0.05);SPP1的表达与癌组织淋巴结转移、分化程度及FIGO具有相关性(P<0.05);OLR1的表达与癌组织淋巴结转移和分化程度具有相关性(P<0.05);在癌组织中,SPP1和OLR1的表达呈正相关(r=0.423,P=0.007)。与SPP1低表达相比,高表达患者的生存率更低(P<0.05);宫颈癌患者的预后不良与SPP1、OLR1的T分期、N分期相关(P<0.05)。结论 宫颈癌组织的发生发展可能与SPP1、OLR1密不可分,检测这两种蛋白对宫颈癌的发生、发展及预后有一定的帮助。

河南省小麦黑胚病优势病原菌链格孢菌(Alternaria spp.)遗传多样性分析

采用RAPD技术对分离自河南省各地的43株小麦黑胚病优势病原菌(Alternariaspp.)菌株进行了遗传多样性分析。17个随机引物共扩增出151条清晰的DNA条带,所扩增出的DNA条带均为多态带,说明河南省小麦黑胚病菌存在着丰富的遗传多样性。利用NTSYS软件进行了病原菌的聚类分析,结果表明,河南省小麦黑胚病菌主要有2个种,即Alter-naria alternata和A.tenuissima,种间的遗传相似系数的变化幅度为0.62～0.92。来自同一个地区的小麦黑胚病菌菌株基本上聚在了一起,表现出很近的亲缘关系,来自不同地区之间的菌株也可以交叉聚类。病原菌遗传多样性分析进一步验证了形态学的鉴定结果。

链格孢(Alternaria alternata)在枣、梨和苹果果实上的致病性分化研究

链格孢(Alternaria alternata)可侵染多种果树引起产前及产后果实黑斑病,造成严重的经济损失。不同果树来源的链格孢是否能交互传染还不清楚。本文探讨了引起北方枣、梨和苹果果实黑斑病的链格孢在这3种果实上的致病性分化和交互致病性,明确其交互传染特性。从北方不同地区果园采集枣、梨和苹果的黑斑病病果样品,通过组织分离法进行病原菌的分离,然后进行纯化及鉴定。利用离体果实接种法对分离到的各个病菌分别在枣、梨、苹果的果实上进行致病性测定,分析了其致病性的差异。结果显示:从3种病果实上共分离出45株链格孢,其中对枣、梨和苹果表现出强致病性的分别为5、11、7株,表现为弱致病的分别为17、8、20株;对3种果实的致病性均在次强等级以上的有6株,致病性均为弱等级的有3株,而在3种果实上致病性等级表现为次强等级以上的菌株有30株,占到总菌株的66.6%。总之,枣、梨和苹果病果上的链格孢存在致病性分化现象,并在这3种果实上是可以交叉侵染致病,互为侵染源。因此,在病害防治方面,临近的不同果园对黑斑病需联防联治。