To investigate the genetic mechanism of AI-tolerance in soybean, a recombinant inbred line population （RIL） with 184 F2：7：11 lines derived from the cross of Kefen9 No.1×Nannong 1138-2 （AI-tolerant×AI-se...To investigate the genetic mechanism of AI-tolerance in soybean, a recombinant inbred line population （RIL） with 184 F2：7：11 lines derived from the cross of Kefen9 No.1×Nannong 1138-2 （AI-tolerant×AI-sensitive） were tested in pot experiment with sand culture medium in net room in Nanjing. Four traits, i.e. plant height, number of leaves, shoot dry weight and root dry weight at seedling stage, were evaluated and used to calculate the average membership index （FAi） as the indicator of AI-tolerance. The composite interval mapping （CIM） under WinQTL Cartographer v. 2.5 detected five QTLs （i.e. qFAi-1, qFAi- 2, qFAi-3, qFAi-4 and qFAi-5）, explaining 5.20%-9.07% of the total phenotypic variation individually. While with the multiple interval mapping （MIM） of the same software, five QTLs （qFAi-1, qFAi.5, qFAi.6, qFAi-7, and qFAi-8） explaining 5.7%-24.60% of the total phenotypic variation individually were mapped. Here qFAi-1 and qFAi-5 were detected by both CIM and MIM with the locations in a same flanking marker region, GMKF046-GMKF080 on B1 and satt278-sat_95 on L, respectively. While qFAi-2 under CIM and qFAi-6 under MIM both on Dlb2 were located in neighboring regions with their confidence intervals overlapped and might be the same locus. Segregation analysis under major gene plus polygene inheritance model showed that AI-tolerance was controlled by two major genes （h^2mg=33.05%） plus polygenes （h^2pg=52.73%）. Both QTL mapping and segregation analysis confirmed two QTLs responsible for AI-tolerance with relatively low heritability, and there might be a third QTL, confounded with the polygenes in segregation analysis.展开更多
Background: Primary focal hyperhidrosis can significantly reduce quality of life. Therefore a lot of treatment options in a range of conservative, physical and surgical techniques are available. Objective: To assess t...Background: Primary focal hyperhidrosis can significantly reduce quality of life. Therefore a lot of treatment options in a range of conservative, physical and surgical techniques are available. Objective: To assess the efficacy of an antiperspirant containing aluminum chloride compared to a Botulinumtoxin A treatment for patients with primary focal hyperhidrosis. Methods and material: In this randomized, single-center, half-side trail, a clinical score was done by patients and physician to evaluate the severity of hyperhidrosis. Gravimetric tests were performed to gather the amount of sweat per unit of time. Furthermore the efficacy was determined using a four point questionnaire. Skin irritation was assessed by measuring pH value and transepidermal water loss. Results: A total of 22 patients were enrolled. Two weeks after baseline the hyperhidrosis level was significantly reduced (BTX-A: ﹣92.9%, AL: 66.7%). In addition both treatment options induced a significant reduction of sweat production (BTX-A: ﹣80.8%, AL: 68.8%). Please change in: Patients evaluated good resp, very good efficacy for both treatment options at day 28. pH value and TEWL never left physiological range. Conclusion: Both botulinum toxin A treatment and an antiperspirant containing aluminum chloride in the evaluated galenic formulation are an effective and safe treatment option for axillary focal hyperhidrosis.展开更多
基金the National Natural Science Foundation of China (30490250 and 30671266)the State Key Basic Research and Development Plan of China (2006CB101708)+2 种基金the Hi-Tech Research and Development Program (863) of China (2006AA100104)the National Science and Technology Sup- porting Program (2006BAD13B05-7)the Ministry of Education Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) and the 111 Project (B08025)
文摘To investigate the genetic mechanism of AI-tolerance in soybean, a recombinant inbred line population （RIL） with 184 F2：7：11 lines derived from the cross of Kefen9 No.1×Nannong 1138-2 （AI-tolerant×AI-sensitive） were tested in pot experiment with sand culture medium in net room in Nanjing. Four traits, i.e. plant height, number of leaves, shoot dry weight and root dry weight at seedling stage, were evaluated and used to calculate the average membership index （FAi） as the indicator of AI-tolerance. The composite interval mapping （CIM） under WinQTL Cartographer v. 2.5 detected five QTLs （i.e. qFAi-1, qFAi- 2, qFAi-3, qFAi-4 and qFAi-5）, explaining 5.20%-9.07% of the total phenotypic variation individually. While with the multiple interval mapping （MIM） of the same software, five QTLs （qFAi-1, qFAi.5, qFAi.6, qFAi-7, and qFAi-8） explaining 5.7%-24.60% of the total phenotypic variation individually were mapped. Here qFAi-1 and qFAi-5 were detected by both CIM and MIM with the locations in a same flanking marker region, GMKF046-GMKF080 on B1 and satt278-sat_95 on L, respectively. While qFAi-2 under CIM and qFAi-6 under MIM both on Dlb2 were located in neighboring regions with their confidence intervals overlapped and might be the same locus. Segregation analysis under major gene plus polygene inheritance model showed that AI-tolerance was controlled by two major genes （h^2mg=33.05%） plus polygenes （h^2pg=52.73%）. Both QTL mapping and segregation analysis confirmed two QTLs responsible for AI-tolerance with relatively low heritability, and there might be a third QTL, confounded with the polygenes in segregation analysis.
文摘Background: Primary focal hyperhidrosis can significantly reduce quality of life. Therefore a lot of treatment options in a range of conservative, physical and surgical techniques are available. Objective: To assess the efficacy of an antiperspirant containing aluminum chloride compared to a Botulinumtoxin A treatment for patients with primary focal hyperhidrosis. Methods and material: In this randomized, single-center, half-side trail, a clinical score was done by patients and physician to evaluate the severity of hyperhidrosis. Gravimetric tests were performed to gather the amount of sweat per unit of time. Furthermore the efficacy was determined using a four point questionnaire. Skin irritation was assessed by measuring pH value and transepidermal water loss. Results: A total of 22 patients were enrolled. Two weeks after baseline the hyperhidrosis level was significantly reduced (BTX-A: ﹣92.9%, AL: 66.7%). In addition both treatment options induced a significant reduction of sweat production (BTX-A: ﹣80.8%, AL: 68.8%). Please change in: Patients evaluated good resp, very good efficacy for both treatment options at day 28. pH value and TEWL never left physiological range. Conclusion: Both botulinum toxin A treatment and an antiperspirant containing aluminum chloride in the evaluated galenic formulation are an effective and safe treatment option for axillary focal hyperhidrosis.
