Electroactive hydrogels could guide the regeneration of nerves and promote their functional recovery.An aniline pentamer-crosslinked chitosan(CS-AP)hydrogel with better electroactivity and degradation was fabricated b...Electroactive hydrogels could guide the regeneration of nerves and promote their functional recovery.An aniline pentamer-crosslinked chitosan(CS-AP)hydrogel with better electroactivity and degradation was fabricated by the carbodiimide method,and then injected into the repair site of sciatic nerve damage,with its gelation time,tensile strength,and conductivity reaching 35 min,5.02-6.69 MPa,and from 2.97×10^(-4) to 3.25×10^(-4) S-cm^(-1),respectively,due to the cross-linkage and well-distribution of AP.There was better cytocompativility of CS-AP hydrogel on nerve cells.The results of the in vivo repair indicated that CS-AP10 hydrogel induced the capillaries formation and the repair of sciatic nerve defect,and re-innervated gastrocnemius muscle in the CS-AP10 group were obviously better than other experimental groups,due to the electroactivity of CS-AP and its degradation into fragments.These results indicated the potential application of CS-AP hydrogel in the regeneration and function recovery of peripheral nerve injury.展开更多
Compound B was synthesized through oxidative coupling reaction of p-phenylene-diamine and compound A(acetylamino-capped aniline dimer). This synthetic route protected the N-phenyl-1,4-phenylenediamine from further rea...Compound B was synthesized through oxidative coupling reaction of p-phenylene-diamine and compound A(acetylamino-capped aniline dimer). This synthetic route protected the N-phenyl-1,4-phenylenediamine from further reaction. The pentamer was synthesized by the hydrolysis of compound B(acetylamino-capped aniline pentamer) and was characterized by IR, elemental analysis and MALDI-TOF-MS. The chemical oxidation process of the pentamer was studied by UV-Vis spectra. It was found the pentamer was oxidized to its EB form and then to the pernigraniline oxidation state.展开更多
Every slight movement of human body, such as beat of heart, contract of muscles, thinking of brain, is associated with bioelectricity. In search for a neural conducting tissue engineering material which can {ideally} ...Every slight movement of human body, such as beat of heart, contract of muscles, thinking of brain, is associated with bioelectricity. In search for a neural conducting tissue engineering material which can {ideally} conduct bioelectrical signals in vivo, the block copolymer of polylactide(PLA) and aniline pentamera material which is electro-active, remarkably biocompatible and biodegradable, was designed and synthesized. The aniline pentamer is similar with polyaniline in electrical chemical properties, but more soluble than the latter. The copolymer is prepared by condensation polymerization between carboxyl-capped aniline pentamer and {dihydroxyl}-capped PLA, with N,N′-dicyclohexylcarbodiimide(DCC) as a condensing agent. The co-polymerization of aniline pentamer and PLA can not only reduce the toxicity of aniline pentamer, but also make the {material} easily to degrade and excrete from the body. These properties of the copolymer provide a favorable prospect in biomedical application in vivo. The synthesized material is investigated by FTIR, {}+1H NMR, UV, and cyclic voltammetric measurements.展开更多
基金supported by National Key Research and Development Program of China(Grant No.2018YFC1106800)Sichuan Science and Technology Project(Grant No.2018JY0535).
文摘Electroactive hydrogels could guide the regeneration of nerves and promote their functional recovery.An aniline pentamer-crosslinked chitosan(CS-AP)hydrogel with better electroactivity and degradation was fabricated by the carbodiimide method,and then injected into the repair site of sciatic nerve damage,with its gelation time,tensile strength,and conductivity reaching 35 min,5.02-6.69 MPa,and from 2.97×10^(-4) to 3.25×10^(-4) S-cm^(-1),respectively,due to the cross-linkage and well-distribution of AP.There was better cytocompativility of CS-AP hydrogel on nerve cells.The results of the in vivo repair indicated that CS-AP10 hydrogel induced the capillaries formation and the repair of sciatic nerve defect,and re-innervated gastrocnemius muscle in the CS-AP10 group were obviously better than other experimental groups,due to the electroactivity of CS-AP and its degradation into fragments.These results indicated the potential application of CS-AP hydrogel in the regeneration and function recovery of peripheral nerve injury.
文摘Compound B was synthesized through oxidative coupling reaction of p-phenylene-diamine and compound A(acetylamino-capped aniline dimer). This synthetic route protected the N-phenyl-1,4-phenylenediamine from further reaction. The pentamer was synthesized by the hydrolysis of compound B(acetylamino-capped aniline pentamer) and was characterized by IR, elemental analysis and MALDI-TOF-MS. The chemical oxidation process of the pentamer was studied by UV-Vis spectra. It was found the pentamer was oxidized to its EB form and then to the pernigraniline oxidation state.
文摘Every slight movement of human body, such as beat of heart, contract of muscles, thinking of brain, is associated with bioelectricity. In search for a neural conducting tissue engineering material which can {ideally} conduct bioelectrical signals in vivo, the block copolymer of polylactide(PLA) and aniline pentamera material which is electro-active, remarkably biocompatible and biodegradable, was designed and synthesized. The aniline pentamer is similar with polyaniline in electrical chemical properties, but more soluble than the latter. The copolymer is prepared by condensation polymerization between carboxyl-capped aniline pentamer and {dihydroxyl}-capped PLA, with N,N′-dicyclohexylcarbodiimide(DCC) as a condensing agent. The co-polymerization of aniline pentamer and PLA can not only reduce the toxicity of aniline pentamer, but also make the {material} easily to degrade and excrete from the body. These properties of the copolymer provide a favorable prospect in biomedical application in vivo. The synthesized material is investigated by FTIR, {}+1H NMR, UV, and cyclic voltammetric measurements.
