A laboratory-scale anaerobic sequencing batch reactor (ASBR) was used to pretreat coking wastewater. Inoculated anaerobic granular biomass was acclimated for 225 d to the coking wastewater, and then the biochemical me...A laboratory-scale anaerobic sequencing batch reactor (ASBR) was used to pretreat coking wastewater. Inoculated anaerobic granular biomass was acclimated for 225 d to the coking wastewater, and then the biochemical methane potential (BMP)of the coking wastewater in the acclimated granular biomass was measured. At the same time, some fundamental technological factors, such as the filling time and the reacting time ratio (tf/tr), the mixing intensity and the intermittent mixing mode, that affect anaerobic pretreatment of coking wastewater with ASBR, were evaluated through orthogonal tests. The COD removal efficiency reached 38％～50％ in the stable operation period with the organic loading rate of 0.37～0.54 kg COD/(m3.d) at the optimum conditions of tf/tr, the mixing intensity and the intermittent mixing mode. In addition, the biodegradability of coking wastewater distinctly increased after the pretreatment using ASBR. At the end of the experiment, the microorganism forms on the granulated sludge in the ASBR were observed using SEM (scanning electron microscope) and fluoroscope. The results showed that the dominant microorganism on the granular sludge was Methanosaeta instead of Methanosarcina dominated on the inoculated sludge.展开更多
The present lab-scale research reveals the enrichment of anaerobic ammonium oxidation microorganism from methanogenic anaerobic granular sludge and the effect of inorganic carbon(sodium bicarbonate)on anaerobic ammoni...The present lab-scale research reveals the enrichment of anaerobic ammonium oxidation microorganism from methanogenic anaerobic granular sludge and the effect of inorganic carbon(sodium bicarbonate)on anaerobic ammonium oxidation.The enrichment of anammox bacteria was carried out in a 7.0-L sequencing batch reactor(SBR)and the effect of bicarbonate on anammox was conducted in a 3.0-L SBR.Research results,especially the biomass,showed first signs of anammox activity after 54 d cultivation with synthetic wast...展开更多
In this study,experiments were designed to reveal in-depth information of the effect of pH and hydraulic retention time（HRT）on biohydrogen fermentation from liquid swine manure supplemented with glucose using an Ana...In this study,experiments were designed to reveal in-depth information of the effect of pH and hydraulic retention time（HRT）on biohydrogen fermentation from liquid swine manure supplemented with glucose using an Anaerobic Sequencing Batch Reactor（ASBR）System.Five values of HRT（8,12,16,20,and 24 h）were first tested and the best HRT determined was further studied at five p H levels（4.4,4.7,5.0,5.3,and 5.6）.The results showed that for HRT 24 h,there was a dividing H2 content（around 37%）related to the total biogas production rate for the ASBR System running at p H 5.0.When the H2 content went beyond 37%,an appreciable decline in biogas production rate was observed,implying that there might exist an H2 content limit in the biogas.For other HRTs（8 through 20 h）,an average H2 content of 42%could be achieved.In the second experiment（HRT 12 h）,the highest H2 content（35%）in the biogas was found to be associated with p H 5.0.The upswing of p H from 5.0 to 5.6 had a significantly more impact on biogas H2 content than the downswing of p H from5.0 to 4.3.The results also indicated good linear relationships of biogas and H2 production rates with HRT（r=0.9971 and0.9967,respectively）.Since the optimal ASBR operating conditions were different for the biogas/H2 production rates and the H2 yield,a compromised combination of the running parameters was determined to be HRT 12 h and pH 5.0 in order to achieve good biogas/H2 productions.展开更多
The performance of SBRs treating two kinds of wastewater(synthetic wastewater con- taining polyvinyl alcohol and effluent from a coke-plant wastewater treatment system)was investi- gated in this study,in order to exam...The performance of SBRs treating two kinds of wastewater(synthetic wastewater con- taining polyvinyl alcohol and effluent from a coke-plant wastewater treatment system)was investi- gated in this study,in order to examine the exact function of anaerobic portion in a conventional SBR.The set up of 4-or 8-hour anaerobic mixing period in a SBR's cycle did not benefit for PVA degradation.While an anaerobic reactor seeded with anaerobic sludge could partly hydrolyse and acidify PVA into readily-degradable intermediates.During the anaerobic fill period of an SBR treat- ing the effluent from a coke-plant wastewater treatment system,the organic concentration was re- duced to certain extent due to the adsorption of activated sludge and dilution of the mixed liquor from the previous cycle.Parts of readily-degradable organics in the influent were utilised by denitri- fiers as carbon source.The biomass in a conventional SBR was alternatively imposed to aerobic and anaerobic conditions in its operating cycle,the environmental conditions needed for anaerobic hy- drolization and acidification of refractory organics could not occur in such an SBR.展开更多
Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges.In this study,we applied an anaerobic sequencing batch reactor(AnSBR)to treat protein-rich ...Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges.In this study,we applied an anaerobic sequencing batch reactor(AnSBR)to treat protein-rich wastewater from a slaughterhouse.We focused on identifying the key factors influencing the removal of chemical oxygen demand(COD)and the settling performance of the sludge.The AnSBR achieved a maximum total COD removal of 90%,a protein degradation efficiency exceeding 80%,and a COD to methane conversion efficiency of over 70%at organic loading rates of up to 6.2 g COD L^(-1)d^(-1).We found that the variations in both the organic loading rate within the reactor and the hydraulic retention time in the buffer tank had a significant effect on COD removal.The hydraulic retention time in the buffer tank and the reactor,which determined the ammonification efficiencies and the residual carbohydrate concentrations in the reactor liquid,affected the sludge settleability.Furthermore,the genus Clostridium sensu stricto 1,known as protein-and lipids-degraders,was predominant in the reactor.Statistical analysis showed a significant correlation between the core microbiome and ammonification efficiency,highlighting the importance of protein degradation as the governing process in the treatment.Our results will provide valuable insights to optimise the design and operation of AnSBR for efficient treatment of protein-rich wastewater.展开更多
In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewat...In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewater containing three commercial reactive azo dyes was considered.Two stage processes performed better than one stage ones,both in terms of overall organic and color removal,as well as the higher contribution of anaerobic stage to the overall removal performance,thereby making them a more energy efficient option.The employment of a moving bed sequencing batch biofilm reactor,which uses both suspended and attached biomass,for the implementation of the anaerobic stage of the process,was compared with a sequencing batch reactor that only employs suspended biomass.The results showed that,although there was no meaningful difference in biomass concentration between the two bioreactors,the latter reactor had better performance in terms of chemical oxygen demand(COD)removal efficiency and rate and color removal rate.Further exploratory tests revealed a difference between the roles of suspended and attached bacterial populations,with the former yielding better color removal whilst the latter had better COD removal performance.The sequential anaerobic–aerobic process,employing an aerobic membrane bioreactor in the aerobic stage resulted in COD and color removal of 77.1±7.9%and 79.9±1.5%,respectively.The incomplete COD and color removal was attributed to the presence of soluble microbial products in the effluent and the autoxidation of dye reduction metabolites,respectively.Also,aerobic partial mineralization of the dye reduction metabolites,was experimentally observed.展开更多
The feasibility of the nitrous organic wastewater treated was studied in seven anaerobic sequencing batch reactors（ASBRs） （0^#-6^#） which had been run under stable anaerobic ammonium oxidation （Anammox）. By mean...The feasibility of the nitrous organic wastewater treated was studied in seven anaerobic sequencing batch reactors（ASBRs） （0^#-6^#） which had been run under stable anaerobic ammonium oxidation （Anammox）. By means of monitoring and data analysis of COD, NH4^#-N, NO2^--N, NO3^--N and pH, and of microbial test, the results revealed that the optimal Anammox performance was achieved from 2^# reactor in which COD/NH4^＋ -N was 1.65, Anammox bacteria and denitrification bacteria could coexist, and Anammox reaction and denitrification reaction could occur simultaneously in the reactors. The ratio of NH4^＋-N consumed ： NO2^- -N consumed ： NO3^- -N produced was 1：1.38：0.19 in 0^# reactor which was not added glucose in the wastewater. When different ratio of COD and NH4^＋-N was fed for the reactors, the ratio of NO2^- -N consumed： NH4^＋-N consumed was in the range of 1.51-2.29 and the ratio of NO;-N produced： NH4^＋ -N consumed in the range of 0 -0.05.展开更多
基金Project (No. 40272108) supported by the National Natural Science Foundation of China
文摘A laboratory-scale anaerobic sequencing batch reactor (ASBR) was used to pretreat coking wastewater. Inoculated anaerobic granular biomass was acclimated for 225 d to the coking wastewater, and then the biochemical methane potential (BMP)of the coking wastewater in the acclimated granular biomass was measured. At the same time, some fundamental technological factors, such as the filling time and the reacting time ratio (tf/tr), the mixing intensity and the intermittent mixing mode, that affect anaerobic pretreatment of coking wastewater with ASBR, were evaluated through orthogonal tests. The COD removal efficiency reached 38％～50％ in the stable operation period with the organic loading rate of 0.37～0.54 kg COD/(m3.d) at the optimum conditions of tf/tr, the mixing intensity and the intermittent mixing mode. In addition, the biodegradability of coking wastewater distinctly increased after the pretreatment using ASBR. At the end of the experiment, the microorganism forms on the granulated sludge in the ASBR were observed using SEM (scanning electron microscope) and fluoroscope. The results showed that the dominant microorganism on the granular sludge was Methanosaeta instead of Methanosarcina dominated on the inoculated sludge.
文摘The present lab-scale research reveals the enrichment of anaerobic ammonium oxidation microorganism from methanogenic anaerobic granular sludge and the effect of inorganic carbon(sodium bicarbonate)on anaerobic ammonium oxidation.The enrichment of anammox bacteria was carried out in a 7.0-L sequencing batch reactor(SBR)and the effect of bicarbonate on anammox was conducted in a 3.0-L SBR.Research results,especially the biomass,showed first signs of anammox activity after 54 d cultivation with synthetic wast...
基金the funding for this project was provided by the Minnesota Environment and Natural Resources Trust Fund as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR),USA
文摘In this study,experiments were designed to reveal in-depth information of the effect of pH and hydraulic retention time（HRT）on biohydrogen fermentation from liquid swine manure supplemented with glucose using an Anaerobic Sequencing Batch Reactor（ASBR）System.Five values of HRT（8,12,16,20,and 24 h）were first tested and the best HRT determined was further studied at five p H levels（4.4,4.7,5.0,5.3,and 5.6）.The results showed that for HRT 24 h,there was a dividing H2 content（around 37%）related to the total biogas production rate for the ASBR System running at p H 5.0.When the H2 content went beyond 37%,an appreciable decline in biogas production rate was observed,implying that there might exist an H2 content limit in the biogas.For other HRTs（8 through 20 h）,an average H2 content of 42%could be achieved.In the second experiment（HRT 12 h）,the highest H2 content（35%）in the biogas was found to be associated with p H 5.0.The upswing of p H from 5.0 to 5.6 had a significantly more impact on biogas H2 content than the downswing of p H from5.0 to 4.3.The results also indicated good linear relationships of biogas and H2 production rates with HRT（r=0.9971 and0.9967,respectively）.Since the optimal ASBR operating conditions were different for the biogas/H2 production rates and the H2 yield,a compromised combination of the running parameters was determined to be HRT 12 h and pH 5.0 in order to achieve good biogas/H2 productions.
文摘The performance of SBRs treating two kinds of wastewater(synthetic wastewater con- taining polyvinyl alcohol and effluent from a coke-plant wastewater treatment system)was investi- gated in this study,in order to examine the exact function of anaerobic portion in a conventional SBR.The set up of 4-or 8-hour anaerobic mixing period in a SBR's cycle did not benefit for PVA degradation.While an anaerobic reactor seeded with anaerobic sludge could partly hydrolyse and acidify PVA into readily-degradable intermediates.During the anaerobic fill period of an SBR treat- ing the effluent from a coke-plant wastewater treatment system,the organic concentration was re- duced to certain extent due to the adsorption of activated sludge and dilution of the mixed liquor from the previous cycle.Parts of readily-degradable organics in the influent were utilised by denitri- fiers as carbon source.The biomass in a conventional SBR was alternatively imposed to aerobic and anaerobic conditions in its operating cycle,the environmental conditions needed for anaerobic hy- drolization and acidification of refractory organics could not occur in such an SBR.
基金supported by Biothane Veolia Water Technologies Techno Center,KWR Water Research Institute and Hydrobusiness B.V as part of the TKI Project Innovative Slaughterhouse Wastewater Treatment TechnologyThis research was co-financed with PPS-funding from the Top consortia for Knowledge&Innovation(TKI’s)of the Dutch Ministry of Economic Affairs and Climate PolicyThe authors would like to thank China Scholarship Council for the State Scholarship Fund(No.201708450043)granted to the first author.
文摘Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several challenges.In this study,we applied an anaerobic sequencing batch reactor(AnSBR)to treat protein-rich wastewater from a slaughterhouse.We focused on identifying the key factors influencing the removal of chemical oxygen demand(COD)and the settling performance of the sludge.The AnSBR achieved a maximum total COD removal of 90%,a protein degradation efficiency exceeding 80%,and a COD to methane conversion efficiency of over 70%at organic loading rates of up to 6.2 g COD L^(-1)d^(-1).We found that the variations in both the organic loading rate within the reactor and the hydraulic retention time in the buffer tank had a significant effect on COD removal.The hydraulic retention time in the buffer tank and the reactor,which determined the ammonification efficiencies and the residual carbohydrate concentrations in the reactor liquid,affected the sludge settleability.Furthermore,the genus Clostridium sensu stricto 1,known as protein-and lipids-degraders,was predominant in the reactor.Statistical analysis showed a significant correlation between the core microbiome and ammonification efficiency,highlighting the importance of protein degradation as the governing process in the treatment.Our results will provide valuable insights to optimise the design and operation of AnSBR for efficient treatment of protein-rich wastewater.
基金supported by Takmiliran textile dyeing factory(272219601)Materials and Energy Research Center(MERC)(99392003).
文摘In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewater containing three commercial reactive azo dyes was considered.Two stage processes performed better than one stage ones,both in terms of overall organic and color removal,as well as the higher contribution of anaerobic stage to the overall removal performance,thereby making them a more energy efficient option.The employment of a moving bed sequencing batch biofilm reactor,which uses both suspended and attached biomass,for the implementation of the anaerobic stage of the process,was compared with a sequencing batch reactor that only employs suspended biomass.The results showed that,although there was no meaningful difference in biomass concentration between the two bioreactors,the latter reactor had better performance in terms of chemical oxygen demand(COD)removal efficiency and rate and color removal rate.Further exploratory tests revealed a difference between the roles of suspended and attached bacterial populations,with the former yielding better color removal whilst the latter had better COD removal performance.The sequential anaerobic–aerobic process,employing an aerobic membrane bioreactor in the aerobic stage resulted in COD and color removal of 77.1±7.9%and 79.9±1.5%,respectively.The incomplete COD and color removal was attributed to the presence of soluble microbial products in the effluent and the autoxidation of dye reduction metabolites,respectively.Also,aerobic partial mineralization of the dye reduction metabolites,was experimentally observed.
文摘The feasibility of the nitrous organic wastewater treated was studied in seven anaerobic sequencing batch reactors（ASBRs） （0^#-6^#） which had been run under stable anaerobic ammonium oxidation （Anammox）. By means of monitoring and data analysis of COD, NH4^#-N, NO2^--N, NO3^--N and pH, and of microbial test, the results revealed that the optimal Anammox performance was achieved from 2^# reactor in which COD/NH4^＋ -N was 1.65, Anammox bacteria and denitrification bacteria could coexist, and Anammox reaction and denitrification reaction could occur simultaneously in the reactors. The ratio of NH4^＋-N consumed ： NO2^- -N consumed ： NO3^- -N produced was 1：1.38：0.19 in 0^# reactor which was not added glucose in the wastewater. When different ratio of COD and NH4^＋-N was fed for the reactors, the ratio of NO2^- -N consumed： NH4^＋-N consumed was in the range of 1.51-2.29 and the ratio of NO;-N produced： NH4^＋ -N consumed in the range of 0 -0.05.
