GIS-Based Multi-Criteria Decision Analysis (MCDA) and Analytical Hierarchy Process (AHP) Techniques to Derive Flood Risks Management on Rice Productivity in Gishari Marshland

Floods are phenomenon with significant socio-economic implications mainly for human loss, agriculture, livestock, soil loss and land degradation, for which many researchers try to identify the most appropriate methodologies by analyzing their temporal and spatial development. This study therefore attempts to employ the GIS-based multi-criteria decision analysis and analytical hierarchy process techniques to derive the flood risks management on rice productivity in the Gishari Agricultural Marshland in Rwamagana district, Rwanda. Here, six influencing potential factors to flooding, including river slope, soil texture, Land Use Land Cover through Land Sat 8, rainfall, river distance and Digital Elevation Model are considered for the delineation of flood risk zones. Data acquisition like Landsat 8 images, DEM, land use land cover, slope, and soil class in the study area were considered. Results showed that if the DEM is outdated or inaccurate due to changes in the terrain, such as construction, excavation, or erosion, the predicted flood patterns might not reflect the actual water flow. This could result unexpected flood extents and depths, potentially inundating rice fields that were not previously at risk and this, expectedly explained that the increase 1 m in elevation would reduce the rice productivity by 0.17% due to unplanned flood risks in marshland. It was found that the change in rainfall distribution in Gishari agricultural marshland would also decrease the rice productivity by 0.0018%, which is a sign that rainfall is a major factor of flooding in rice scheme. Rainfall distribution plays a crucial role in flooding analysis and can directly impact rice productivity. Oppositely, another causal factor was Land Use Land Cover (LULC), where the Multivariate Logistic Regression Model Analysis findings showed that the increase of one unit in Land Use Land Cover would increase rice productivity by 0.17% of the total rice productivity from the Gishari Agricultural Marshland. Based on findings from these techniques, the Gishari Agricultural Marshlands having steeped land with grassland is classified into five classes of flooding namely very low, low, moderate, high, and very high which include 430%, 361%, 292%, 223%, and 154%. Government of Rwanda and other implementing agencies and major key actors have to contribute on soil and water conservation strategies to reduce the runoff and soil erosion as major contributors of flooding.

The suitability assessment on site selection for bottom seeding scallop culture based on analytic hierarchy process

Scallop culture is an important way of bottom-seeding marine ranching,which is of great significance to improve the current situation of fishery resources.However,there are some problems in site-selection evaluation of marine ranching,such as imperfect criteria system,complex structure,untargeted criteria quantification,etc.In addition,no site-selection evaluation method of bottom-seeding culture areas for scallops is available.Therefore,we established a hierarchy structure model according to the analytic hierarchy process(AHP)theory,in which social,physical,chemical,and biological environments are used as main criteria,and marine functional zonation,water depth,current,water temperature,salinity,substrate type,water quality,sediment quality,red tide,phytoplankton,and zooplankton are used as sub-criteria,on which a multi-parameter evaluation system is set up.Meanwhile,the dualism method,assignment method,and membership function method were used to quantify sub-criteria,and a quantitative evaluation for the entire criteria was added,including the evaluation and analysis of two types of unsuitable environmental situations.By overall consideration in scallop yield,quality,and marine ranching construction objectives,the weight of the main criteria could be determined.Five grades in the suitability corresponding to the evaluation result were divided,and the Python language was used to create an evaluation system for efficient calculation and intuitive presentation of the evaluation outcome.Eight marine cases were simulated based on existing survey data,and the results prove that the method is feasible for evaluating and analyzing the site selection of bottom-seeding culture areas for scallops under various environmental situations.The proposed evaluation method can be promoted for the site selection of bottom-seeding marine ranching.This study provided theoretical and methodological references for the site selection evaluation of other types of marine ranching.

A Quality Evaluation System for Dissertation Based on Fuzzy Analytic Hierarchy Process

A dissertation is a research report or scientific paper written by an author to obtain a certain degree. It reflects postgraduates’ research achievements and the educational quality of an institute, even a country. To construct an optimized quality evaluation system for postgraduate dissertation (QESPD), we summarized the influencing factors and invited 10 experienced specialists to rate and prioritize them based on fuzzy analytic hierarchy process. Four primary indicators (innovation, integrity, scientificity and normativity) and 16 sub-indicators were selected to form the evaluation system. The order of primary indicators by weight, was innovation (0.4269), scientificity (0.2807), integrity (0.1728) and normativity (0.1196). The top five sub-dimensions were theoretical originality, scientific value, data reliability, design rationality and evidence credibility. To demonstrate the effectiveness of the proposed system, a case study was performed. In the case study, it was demonstrated that the established two-index-hierarchy QESPD in this study was a more scientific and reasonable evaluation system worthy of promotion and application.

Analytic Hierarchy Process for Technological Risks in the Process of Innovative Drug Development in China

Objective To identify the critical risks in the process of innovative drug research and development,and to provide reference for improving the efficiency of innovative drug development and risk control in China.Methods Expert investigation and analytic hierarchy process were used to determine the weights of different risks.Results and Conclusion The research and analysis results showed that the risks at different stages of development had different effects on the success rate of drug development,among which the risk at the drug discovery stage influenced the most.In the drug discovery stage,inappropriate target selection had the greatest impact on the success rate of drug development.The lack of appropriate cell tissue or animal models had the greatest impact on the success rate of drug development from the discovery of a compound to the application for clinical trials.The difference in changes between nonclinical and clinical studies had the greatest impact on the success rate of drug development from early clinical studies to pivotal clinical studies.Incorrect dose selection had the greatest impact on the success rate of drug development from pivotal clinical studies to marketing authorization applications.The biggest impact from the marketing authorization application to the approval stage was inadequate communication with regulators.After investigating the weight of risk factors in the process of innovative drug development based on scientific methods,a new perspective for the risk control of new drug development and improving the research and development efficiency is provided.

Research on Detection of Food additives Based on Terahertz Spectroscopy and Analytic Hierarchy Process

Terahertz time-domain spectroscopy is a kind of far-infrared spectroscopy technology,and its spectrum reflects the internal properties of substances with rich physical and chemical information,so the use of terahertz waves can be used to qualitatively identify food additives containing nitrogen elements.Analytic hierarchy process(AHP)was originally used to solve evaluation-type problems,and this paper introduces it into the field of terahertz spectral qualitative analysis,proposes a terahertz time-domain spectral qualitative identification method combined with analytic hierarchy process,and verifies the feasibility of the method by taking four common food additives(xylitol,L-alanine,sorbic acid,and benzoic acid)and two illegal additives(melamine,and Sudan Red No.I)as the objects of study.Firstly,the collected terahertz time-domain spectral data were pre-processed and transformed into a data set consisting of peaks,peak positions,peak numbers and overall trends;then,the data were divided into comparison and test sets,and a qualitative additive identification model incorporating analytic hierarchy process was constructed and parameter optimisation was performed.The results showed that the qualitative identification accuracies of additives based on single factors,i.e.,overall trend,peak value,peak position,and peak number,were 80.23%,70.93%,67.44%,and 40.70%,respectively,whereas the identification accuracy of the analytic hierarchy process qualitative identification method based on multi-factors could be improved to 92.44%.In addition,the fuzzy characterisation of the absorption spectrum data was binarised in the data pre-processing stage and used as the base data for the overall trend,and the recognition accuracy was improved to 94.19%by combining the fuzzy characterisation method of such data with the hierarchical analysis qualitative recognition model.The results show that it is feasible to use terahertz technology to identify different varieties of additives,and this paper constructs a hierarchical analytical qualitative model with better effect,which provides a new means for food additives detection,and the method is simple in steps,with a small demand for samples,which is suitable for the rapid detection of small samples.

Application of GIS based analytical hierarchy process and multicriteria decision analysis methods to identify groundwater potential zones in Jedeb Watershed, Ethiopia

The hydrogeological situation of the study area requires the identification of groundwater potential.Remote sensing and satellite data have proven to be reliable tools for understanding various factors that affect groundwater occurrence and movement.This study employed weighted overlay analysis based on satellite imagery and secondary data to create a thematic map for characterizing groundwater potentials in the study area located within Abbay Basin,Ethiopia.Remote sensing(RS)and GIS-based Fuzzy-Analytical Hierarchy Process methods were utilized to classify groundwater potential(GWP)zones into five categories:Very good,good,moderate,poor,and very poor.The central and eastern parts of the study area were identified as having high(33.186%)and very high(2.351%)groundwater potentials,while the western part exhibited poor and very poor potential areas.The groundwater potential map delineated higher and moderate potentials,suitable for installing shallow and production bores.This research demonstrates the effectiveness of RS and GIS techniques for delineating groundwater potential zones,which can aid in the planning and management of groundwater resources.The research findings have the potential to contribute to the formulation of improved groundwater management programs in the study area.

Assessment and Application of Beach Quality Based on Analytic Hierarchy Process in Yangkou Beach, Qingdao

The whole-beach quality assessment is the basis of building and preserving beautiful beaches.The beach quality assessment index system and assessment standard have been established based on the attributes of beaches(including the width,slope,landform,and types),sorting coefficient,and softness degree of surface sediment.The assessment weight of each index for quality evaluation was analyzed using the analytic hierarchy process,and comprehensive scores of selected beach profiles were calculated in accordance with the light assessment standard.A beach quality evaluation model based on index weight and scores was established in this paper.The factors of 12 profiles of Yangkou Beach in Qingdao City were surveyed to carry out a quality assessment,and the comprehensive scores of each profile were calculated in accordance with the evaluation model.The results showed that the quality of Yangkou Beach can be divided into four ratings:excellent,good,medium,and poor.The excellent-quality area includes a wide and flat dry beach zone and soft,flat,and clean intertidal and subtidal zones covered with well-sorted fine sand,and leisure sports,such as volleyball,running,and swimming,are suitable for tourists.The good-quality area features a slightly narrow and dry beach zone,moderately soft and uneven intertidal and subtidal zones covered with fine sand and a small tidal gully,and a small amount of foreign matter;leisure sports,such as walking and running,are suitable for tourists.This study recommends the building of fixed drainage ditches or underground culverts to reduce the tidal gully.The medium-quality area consisted of a narrow and dry beach zone,moderately soft and uneven intertidal and subtidal zones covered by poorly sorted medium sand,a tide ditch,and a small amount of foreign matter.In this area,walking is suitable for tourists.Sand should be supplemented in the intertidal zone.The poor-quality area contained a very narrow and dry beach zone covered with poor-sorted gravel,a very chaotic intertidal zone with a considerable amount of foreign matters,such as bricks and rocks,wide tidal ditches,and an uneven subtidal zone with some reefs;leisure sports are unsuitable here.Thus,foreign matter and reefs should be removed,and the dry beach zone should be supplemented with sand.Therefore,the beach quality assessment is a very useful tool for building beautiful beaches.

Hazard assessment of landslide disaster using information value method and analytical hierarchy process in highly tectonic Chamba region in bosom of Himalaya

The present study is focused on a comparative evaluation of landslide disaster using analytical hierarchy process and information value method for hazard assessment in highly tectonic Chamba region in bosom of Himalaya. During study, the information about the causative factors was generated and the landslide hazard zonation maps were delineated using Information Value Method(IV) and Analytical Hierarchy Process(AHP) using Arc GIS(ESRI). For this purpose, the study area was selected in a part of Ravi river catchment along one of the landslide prone Chamba to Bharmour road corridor of National Highway(NH^(-1)54 A) in Himachal Pradesh, India. A numeral landslide triggering geoenvironmental factors i.e. slope, aspect, relative relief, soil, curvature, land use and land cover(LULC), lithology, drainage density, and lineament density were selected for landslide hazard mapping based on landslide inventory. Landslide hazard zonation map was categorized namely "very high hazard, high hazard, medium hazard, low hazard, and very low hazard". The results from these two methods were validated using Area Under Curve(AUC) plots. It is found that hazard zonation map prepared using information value method and analytical hierarchy process methods possess the prediction rate of 78.87% and 75.42%, respectively. Hence, landslide hazardzonation map obtained using information value method is proposed to be more useful for the study area. These final hazard zonation maps can be used by various stakeholders like engineers and administrators for proper maintenance and smooth traffic flow between Chamba and Bharmour cities, which is the only route connecting these tourist places.

Landslide susceptibility analysis of Karakoram highway using analytical hierarchy process and scoops 3D

Landslides are prevalent,regular,and expensive hazards in the Karakoram Highway(KKH)region.The KKH connects Pakistan with China in the present China-Pakistan Economic Corridor(CPEC)context.This region has not only immense economic importance but also ecological significance.The purpose of the study was to map the landslide-prone areas along KKH using two different techniquesAnalytical Hierarchy Process(AHP)and Scoops 3 D model.The causative parameters for running AHP include the lithology,presence of thrust,land use land cover,precipitation,and Digital Elevation Model(DEM)derived variables(slope,curvature,aspect,and elevation).The AHP derived final landslide susceptibility map was classified into four zones,i.e.,low,moderate,high,and extremely high.Over 80%of the study area falls under the moderate(43%)and high(40%)landslide susceptible zones.To assess the slope stability of the study area,the Scoops 3 D model was used by integrating with the earthquake loading data.The results of the limit equilibrium analysis categorized the area into four groups(low,moderate,high,and extremely high mass)of slope failure.The areas around Main Mantle Thrust(MMT)including Dubair,Jijal,and Kohistan regions,had high volumes of potential slope failures.The results from AHP and Scoops 3 D techniques were validated with the landslides inventory record of the Geological Survey of Pakistan and Google Earth.The results from both the techniques showed similar output that coincides with the known landslides areas.However,Scoops 3 D provides not only susceptible zones but also the range of volume of the potential slope failures.Further,these techniques could be used in other mountainous areas,which could help in the landslide mitigation measures.

Seismic risk evaluation for a planning mountain tunnel using improved analytical hierarchy process based on extension theory

Seismic risk evaluation(SRE) in early stages(e.g., project planning and preliminary design)for a mountain tunnel located in seismic areas has the same importance as that in final stages(e.g.,performance-based design, structural analysis, and optimization). SRE for planning mountain tunnels bridges the gap between the planning on the macro level and the design/analysis on the micro level regarding the risk management of infrastructural systems. A transition from subjective or qualitative description to objective or quantitative quantification of seismic risk is aimed to improve the seismic behavior of the mountain tunnel and thus reduce the associated seismic risk. A new method of systematic SRE for the planning mountain tunnel was presented herein. The method employs extension theory(ET)and an ET-based improved analytical hierarchy process. Additionally, a new risk-classification criterion is proposed to classify and quantify the seismic risk for a planning mountain tunnel. This SRE method is applied to a mountain tunnel in southwest China, using the extension model based on matter element theory and dependent function operation.The reasonability and flexibility of the SRE method for application to the mountain tunnel are illustrated.According to different seismic risk levels and classification criteria, methods and measures for improving the seismic design are proposed, which can reduce the seismic risk and provide a frame of reference for elaborate seismic design.

Material selection of polymeric composite automotive bumper beam using analytical hierarchy process

Selection of materials,as an area of design research,has been under considerable interest over the years.Materials selection is one of the most important activities in the product development process.Inappropriate decision of materials can cause the product to be reproduced or remanufactured.To avoid this circumstance,one of the useful tools that can be employed in determining the most appropriate material is analytical hierarchy process(AHP).To illustrate the application of AHP,six different types of composite materials were considered.The most appropriate one for suitability of use in manufacturing automotive bumper beam was determined by considering eight main selection factors and 12 sub-factors.The AHP analysis reveals that the glass fibre epoxy is the most appropriate material because it has the highest value(25.7%,mass fraction) compared with other materials.The final material is obtained by performing six different scenarios of the sensitivity analysis.It is proved that glass fibre epoxy is the most optimum decision.

Sustainability Assessment and Ranking of Run of the River(RoR) Hydropower Projects Using Analytical Hierarchy Process(AHP):A Study from Western Himalayan Region of India

In the present scenario,tapping the unutilised hydropower potential is one of the highest priorities in developing countries of the world.Special emphasis is being imparted to run of the river(RoR)mode of power generation.However,the governments are now facing the dilemma whether to promote small hydropower projects(SHPs) or encourage large hydropower projects(LHPs).RoR large hydropower projects result into large scale cutting of mountains for constructing tunnels and access roads,generation of huge quantity of muck and large scale impact on flora and fauna due to diversion of rivers/streams.On the other hand,though SHPs are claimed to be greener and more sustainable by a section of researchers and energy planners but,they will be required to be set up in large number to generate equivalent amount of electricity.The aim of this study is to rank the most sustainable installed capacity range of RoR hydropower projects.To achieve this aim,the study proposes the use of quite popular multi-criteria decision making(MCDM)method of Operation Research named Analytical Hierarchy Process.A case study has been presented from Himachal Pradesh,a hydro rich state located in the western Himalayan region.As per sustainability assessment carried out in this study,hydropower projects in the capacity range 1 to 5 MW have been ranked to be the most sustainable.

Analysis of explosion risk factor potential on coal reclaim tunnel facilities by modified analytical hierarchy process

This study focused on developing a risk assessment method for explosion at a coal reclaim tunnel （CRT） facility. The method was developed based on an analytical hierarchy process （AHP）, which is an expert system that quantifies the factors of explosion incidents, based on events and hierarchies. In this paper, the proposed model was modification from original AHP model, specifically modifying the structure from ＂alternative＇s results＂ to ＂total risk-rating＇s results＂. The total risk-rating is obtained by summing up risk-rating of each factor, where the risk-rating is a multiplication product of the risk value by the AHP weighted value. To support decision-making using the expert system, data on the real conditions of the CRT were collected and analyzed. A physical modeling of the CRT with laboratory-scale experiments was carried out to show the impact of a ventilation system in CRT on diluting the methane gas and coal dust, in order to support the quantification of AHP risk value. The criteria to evaluate the risk of explosion was constructed from six components that are： fuel, oxygen, ignition, confinement, dispersion, and monitoring system. Those components had fifty-two factors that serve as sub-components （root causes）. The main causes of explosion in CRT were found to be： mechanical ventilation failure and abnormal ventilation, breakdown of monitoring system, and coal spontaneous-combustion. Assessments of two CRT facilities at Mine A and Mine B were carried out as a case study in order to check the reliability of the developed AHP method. The results showed that the risk rating of Mine A was classified as high and Mine B was classified as medium, which is in a good agreement with the site conditions.

A Genetic Fuzzy Analytical Hierarchy Process Based Projection Pursuit Method for Selecting Schemes of Water Transportation Projects

The optimal selection of schemes of water transportation projects is a process of choosing a relatively optimal scheme from a number of schemes of water transportation programming and management projects, which is of importance in both theory and practice in water resource systems engineering. In order to achieve consistency and eliminate the dimensions of fuzzy qualitative and fuzzy quantitative evaluation indexes, to determine the weights of the indexes objectively, and to increase the differences among the comprehensive evaluation index values of water transportation project schemes, a projection pursuit method, named FPRM-PP for short, was developed in this work for selecting the optimal water transportation project scheme based on the fuzzy preference relation matrix. The research results show that FPRM-PP is intuitive and practical, the correction range of the fuzzy rained is both stable and accurate; preference relation matrix A it produces is relatively small, and the result obtherefore FPRM-PP can be widely used in the optimal selection of different multi-factor decision-making schemes.

The Research on the Impact Factors of the Comprehensive Evaluation of Communication Unit Camps Based on Analytic Hierarchy Process

Currently, the comprehensive assessment of the communication troops’ camp planning project is primarily qualitative, with limited quantitative evaluation. Drawing upon the relevant spirit of the Military Commission’s documents and leveraging the author’s own work experience in branch offices, this article thoroughly explores the factors influencing the comprehensive assessment of the project and proposes quantitative representation methods for these factors. Utilizing the Analytic Hierarchy Process (AHP), a hierarchical structure model and judgment matrix for the evaluation factors of the communication troops’ camp construction planning project are constructed, enabling the determination of the weightage of each factor. This provides a certain level of support and reference for the project approval and management by branch offices, while also offering valuable insights for the approval and management of camp planning and construction projects in other types of troops and battlefield projects.

Lectotype Optimization of Offshore Platforms by Use of Three-Scale Fuzzy Analytical Hierarchy Process

A Three-Scale Fuzzy Analytical Hierarchy Process (T-FAHP) is proposed by introducing the Three-Scale Analytical Hierarchy Process (T-AHP) and the trapezoid fuzzy number. A multi-objective optimization model based on the T-FAHP is presented subsequently, in which many factors influencing the lectotype of offshore platform are taken into account synthetically, such as the original investment, the maintenance, cost, the ability of resisting fatigue and corrosion, the construction period, the threat to the environment, and so on. With this method, the experts can give the relatively precise ranking weight of each index and at the same time the requirement of consistence checking can be met, The result of a calculation example shows that the T-FAHP is practical.

GIS Based Landslide Susceptibility Mapping with Application of Analytical Hierarchy Process in District Ghizer, Gilgit Baltistan Pakistan

District Ghizer is a rugged mountainous territory which experiences several landslides each year. There are 16 major landslide areas and 53 villages that are at high risk to hazards. Keeping in view the severity of natural hazards, the present study was designed to generate landslide susceptibility map based on twelve causative factors viz., slope, aspect, elevation, drainage network, Stream Power Index (SPI), Topographic Wetness Index (TWI), lithological units, fault lines, rainfall, road network, land cover and soil texture. Soil texture was determined by particle size analysis and data for other factors were acquired from freely available sources. Analytical Hierarchy Process (AHP) was employed to identify major landslide causative factors in the district Ghizer. Further, a temporal assessment from 1999 till 2015 was generated to assess the impact of land cover change on landslides. It indicated that the barren soil/ exposed rocks and glaciers have reduced while the vegetation and water classes have shown increment. The total area that lies in moderate to very high landslide susceptible zones was 74.38%, while slope is the main landslide causative factor in the district Ghizer. Validation of the susceptibility map showed 88.1% of the landslides in the study area had occurred in the moderate to very high susceptible zones.

Landfill Siting Using GIS and AHP （Analytical Hierarchy Process）： A Case Study AI-Qasim Qadhaa, Babylon, Iraq

The selection of a landfill site is considered as a complicated task because this process is based on many factors and restrictions. For Al-Qasim Qadhaa, which is situated in the southern part of the Babylon Governorate, Iraq, there is no landfill site in that area that conforms to the scientific criteria for selecting sites for landfill. For this reason, 15 criteria were adopted in this study （groundwater depth, rivers, soil types, agriculture lands use, land use, elevation, slope, gas pipelines, oil pipelines, power lines, roads, railways, urban centers, villages and archaeological sites） using GIS （geographic information system）, which has a large ability to manage input data. In addition, the AHP （analytical hierarchy process） method was used to derive the relative weightings for each criterion using pair-wise comparison. To obtain the suitability index for candidate landfill sites, a weighted linear combination method was used. After combining these methods, two suitable candidate landfill sites, with areas of 2.766 km2 and 2.055 km2, respectively, were found to satisfy the scientific and environmental requirements. The area of these sites can accommodate solid waste from 2020 until 2030 based on the required area, which was 0.702 km2.

Evaluation of Rainwater Harvesting Methods and Structures Using Analytical Hierarchy Process for a Large Scale Industrial Area

In India, with ever increasing population and stress on natural resources, especially water, rejuvenation of rainwater harvesting (RWH) technique which was forgotten over the days is becoming very essential. Large number of RWH methods that are available in the literature are demand specific and site specific, since RWH system depends on the topography, land use, land cover, rainfall and demand pattern. Thus for each and every case, a detailed evaluation of RWH structures is required for implementation, including the analy-sis of hydrology, topography and other aspects like site availability and economics, however a common methodology could be evolved. The present study was aimed at evaluation of various RWH techniques in order to identify the most appropriate technique suitable for a large scale industrial area to meet its daily wa-ter demand. An attempt is made to determine the volume of water to be stored using mass balance method, Ripple diagram method, analytical method, and sequent peak algorithm method. Based on various satisfying criteria, analytical hierarchy process (AHP) is employed to determine the most appropriate type of RWH method and required number of RWH structures in the study area. If economy alone is considered along with hydrological and site specific parameters, recharging the aquifer has resulted as a better choice. However other criteria namely risk, satisfaction in obtaining required volume of water for immediate utilization etc. has resulted in opting for concrete storage structures method. From the results it is found that AHP, if used with all possible criteria can result in a better tool for evaluation of RWH methods and structures. This RWH structures not only meets the demand but saves transportation cost of water and reduces the dependability of the industry on irrigation reservoir. Besides monetary benefits it is hoped that the micro environment inside the industry will improve due to the cooling effect of the stored water.

UV/Vis-based process analytical technology to improve monoclonal antibody and host cell protein separation

Process analytical technology(PAT) is gaining more interest in the biomanufacturing industry because of its potential to improve operational control and compliance through real-time quality assurance.Currently, biopharmaceutical producers mainly monitor chromatographic processes with ultraviolet/visible(UV/Vis) absorbance. However, this measurement has a very limited correlation with purity and quantity. The current study aims to determine the concentration of monoclonal antibody(mAb) and host cell proteins(HCPs) using a build-in UV/Vis monitoring during Protein A affinity chromatography and to optimize the separation conditions for high purity of mAb and minimizing the HCPs content. The eluate was analyzed through in-line UV/Vis at 280 and 410 nm, representing mAb and HCPs concentration,respectively. Each 0.1 column volume(CV) fraction of UV/Vis chromatogram peak area were calculated,and different separation conditions were then compared. The optimum conditions of mAb separation were found as 12 CV loading, elution at pH 3.5, and starting the collection at 0.5 CV point, resulting in high m Ab recovery of 95.92% and additional removal of 49.98% of HCP comparing with whole elution pool. This study concluded that UV/Vis-based in-line monitoring at 280 and 410 nm showed a high potential to optimize and real-time control Protein A affinity chromatography for mAb purification from HCPs.