Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

Background Anethole(AN)is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in mammals.However,no study has examined the effect of AN on porcine embryonic development.Therefore,we investigated the effect of AN on the development of porcine embryos and the underlying mechanism.Results We cultured porcine in vitro-fertilized embryos in medium with AN(0,0.3,0.5,and 1 mg/mL)for 6 d.AN at 0.5 mg/mL significantly increased the blastocyst formation rate,trophectoderm cell number,and cellular survival rate compared to the control.AN-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the control.Moreover,AN significantly improved the quantity of mitochondria and mitochondrial membrane potential,and increased the lipid droplet,fatty acid,and ATP levels.Interestingly,the levels of proteins and genes related to the sonic hedgehog(SHH)signaling pathway were significantly increased by AN.Conclusions These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.

Synthesis and biological evaluation of lipid-soluble prodrugs of anethole dithiolthione

16 ADT carboxylate esters were prepared by means of esterification and these compounds were expected to increase the bioavailability of 4-hydroxyanehole trithione.In vivo studies showed that ADT concentration of 3a in plasma was much higher than that of ATT during 120 min.Compound 3a could reach blood peak values of ADT at 660.6 ng/mL which was about 14 times of that by ATT.Additionally,the acute toxicity assay indicated high safety of compound 3a that the maximum tolerated dose was no less than 3.25 g/kg.

广西八角挥发油的提取研究与GC-MS分析

In this paper,the Illilium verum Hook.f.grow at mountain of Dayao of Jinxiu in Guangxi,Volatile oil was extracted by soxhet method and steam distillation.All the volatile oil was analyzed by GC-MS,the result show the ingredients and rate of product were change greatly,The major chemical constituents were trans-anethole,estragole and anisaldehyde etc.

Growth performance, nutrient digestibility, antioxidant capacity,blood biochemical biomarkers and cytokines expression in broiler chickens fed different phytogenic levels

The effects of inclusion levels of a phytogenic feed additive(PFA), characterized by menthol anethol and eugenol, on broiler growth performance, nutrient digestibility, biochemical biomarkers and total antioxidant capacity(TAC) of plasma and meat, as well as on the relative expression of selected cytokines,were studied in a 42-d experiment. A total of 225 one-day-old male Cobb broiler chickens were assigned into 3 treatments, with 5 replicates of 15 chickens each. Chickens were fed maize-soybean meal basal diets following a 3 phase(i.e., starter, grower and finisher) feeding program. Depending on PFA inclusion level, treatments were: no PFA(PFA-0), PFA at 100 mg/kg(PFA-100) and PFA at 150 mg/kg(PFA-150).Feed and water were available ad libitum. Feed conversion ratio(FCR) during finisher phase was improved quadratically(P < 0.05) with increasing PFA level. Overall, increasing PFA level increased body weight gain(BWG) in a linear(P < 0.05) and quadratic(P < 0.05) manner with treatments PFA-100 and PFA-150 being greater(P < 0.05) compared with PFA-0. Total tract apparent digestibility of dry matter increased linearly(P < 0.05) and quadratically(P < 0.05) with increasing PFA level. The apparent metabolizable energy corrected for nitrogen(AMEn) also increased linearly(P < 0.05). Increasing PFA level resulted in a linear(P < 0.05) increase in blood plasma TAC. Expression of pro-inflammatory cytokine interleukin-18(IL-18) was reduced linearly(P < 0.05) in spleen with increasing PFA level. In conclusion, PFA inclusion at 100 mg/kg diet positively influenced performance, whereas PFA inclusion at150 mg/kg resulted in a stronger improvement in AMEn and plasma TAC. Finally, PFA inclusion resulted in a pattern of reduced pro-inflammatory biomarker IL-18 at spleen. Overall, this study provides evidence for the beneficial role of PFA as a natural growth and health promoter in broiler chickens that needs to be further confirmed in field studies.