[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to inve...[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS ＋ TDZ 4.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS ＋ TDZ 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS ＋ ZT 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.展开更多
[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flo...[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flower variety of A.andraeanum were used as explants,in order to investigate the influences of the explants selection,disinfection conditions and different media on the callus induction,proliferation and rooting.[Result] The leaf stalk of A.andraeanum was the optimum explant,with the highest callus induction rate（78.3%）.The best disinfecting effect could be obtained when the explants was sterilized with 0.1% HgCl2 for 8 min.The optimum media for the callus induction of Ping Champion and MIDORI were respectively 1/2 MS＋1.50 mg/L 6-BA＋0.50 mg/L 2,4-D＋0.10 mg/L NAA and 1/2 MS＋1.50 mg/L 6-BA＋1.00 mg/L KT＋0.10 mg/L 2,4-D,while the optimum media for callus subculture of Ping Champion and MIDORI were respectively MS＋1.00 mg/L 6-BA＋0.50 mg/L NAA＋0.30 mg/L 2,4-D and MS＋0.50 mg/L 6-BA＋0.50 mg/L KT＋0.10 mg/L NAA.It was beneficial for the proliferation and growth of cluster buds while 10%（w/v）of coconut water or banana jam was added to MS medium at the proliferation stage,and the proliferation rate could be increased by 2.83 fold.At the rooting stage,the medium 1/2 MS＋0.10 mg/L NAA＋0.10 mg/L IAA was the optimum medium for rooting,and the rooting rate was up to 100%.[Conservation] The research results will lay a foundation for the establishment of the industrial seedling breeding system of A.andraeanum,and it will be significant for the development of A.andraeanum industry.展开更多
This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture te...This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.展开更多
At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products...At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products have significantly affected human life. Anthurium andraeanum is the second major tropical potted flower and its transgenic breeding has a promising prospect of application. In this paper, acceptors, transformation methods and introduced exogenous genes （ including reporter genes, selectable marker genes and target genes） of Anthurium andraeanum were summarized; in addition, several issues related to transforma- tion of Anthurium andraeanum were analyzed, aiming at providing reference for transgenic breeding of Anthurium andraeanum.展开更多
[Objectives] This study was conducted to explore the color variation mechanism of Anthurium andraeanum spathe at the protein level. [Methods]The differential proteins of wild type and its white mutant were separated a...[Objectives] This study was conducted to explore the color variation mechanism of Anthurium andraeanum spathe at the protein level. [Methods]The differential proteins of wild type and its white mutant were separated and identified by using one-dimensional gel electrophoresis and mass spectrometry( 1-DE/MS). [Results] Compared with leaves and spadices,the 1-DE patterns of two kinds of spathe proteins were significantly different,and two different bands were detected in wild type spathes and mutant spathes respectively. The four significantly differential bands were selected and analyzed by mass spectrometry,and 138,111,70 and 427 proteins were identified respectively. The results of GO functional annotation analysis showed that the molecular functions of the proteins were mainly catalytic activity and binding,and the main biological processes involved were cellular process and metabolic process. Many proteins involved in the synthesis of anthocyanins and flavonoids,sugar metabolism and some resistance proteins were screened,indicating that the spathe color difference of A. andraeanum‘Pink champion'is not only related to anthocyanin anabolism,but also regulated by various metabolic pathways. [Conclusions]The study provides a new experimental basis for elucidating the molecular mechanism of the regulation of A. andraeanum flower color.展开更多
In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level, the leaves, inflorescences and spathes of the wild type and two mutants of A. andraeanum 'Madural' w...In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level, the leaves, inflorescences and spathes of the wild type and two mutants of A. andraeanum 'Madural' were used as research objects in which the differential expression of proteins related to flower color mutants was analyzed by one-dimensional electrophoresis and mass spectrometry(1-DE/MS). The 1-DE patterns showed that the protein components expressed highly in spathes were mainly concentrated in the molecular weight range of 20-42 kD, and differential bands were detected between the wild type and the mutant, while no significant differences were detected in the leaf and inflorescence proteins. According to the results of mass spectrometry analysis of the differential bands, 21 known functional proteins involved in life processes such as glucose metabolism, resistance, cytoskeleton, gene regulation and signal transduction were identified. It showed that in addition to the influences from anthocyanidins, the spathe color variation of A. andraeanum 'Madural' is also regulated by a variety of metabolic pathway-related proteins.展开更多
An 888-bp ful-length ascorbate peroxidase （APX） complementary DNA （cDNA） gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region,...An 888-bp ful-length ascorbate peroxidase （APX） complementary DNA （cDNA） gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame （ORF）. This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting ofα-helixes,β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction （RT-PCR） analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage （EL） and malondialdehyde （MDA） content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cellmembrane damage and ultimately enhance the plant cold tolerance.展开更多
文摘[Objective] This research aimed to optimize continuously the highly efficient regeneration system of Anthurium andraeanum. [Method] The leaves and petioles of four A. andraeanum varieties were used as explants to investigate the differences in primary callus induction among different A. andraeanum varieties. [Result]The callus formation capacity of SAM and SST was stronger than that of SDM and SHG. Among the four varieties, the leaf regeneration capacity of SAM, SDM and SHG was stronger than the corresponding petiole regeneration capacity. However,the petiole regeneration capacity of SST was stronger. The optimum medium for petiole callus induction of SST was 1/2 MS ＋ TDZ 4.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate of 87.5%; the optimum medium for leaf callus induction of SAM was 1/2 MS ＋ TDZ 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rate more than90%; the optimum medium for leaf callus induction of SDM and SHG was all 1/2MS ＋ ZT 2.0 mg/L ＋ 2, 4-D 0.2 mg/L with induction rates of 59.34% and 79.63%,respectively. [Conclusion] In addition to variety differences, the differences in differentiation ability among different types of calluses should be also taken into account in the establishment and optimization of tissue culture and rapid propagation technology system of A. andraeanum.
基金Supported by National Science and Technology Supporting Project(2007BAD45B07)Key Technology Research and Development Project of Hainan Province(080201,2009YFZX003)~~
文摘[Objective] The aim was to study the techniques for the tissue culture,rapid propagation and transplantation of Anthurium andraeanum.[Method] The leaf,leaf stalk,spathe and spadix of the pot flower variety and cut flower variety of A.andraeanum were used as explants,in order to investigate the influences of the explants selection,disinfection conditions and different media on the callus induction,proliferation and rooting.[Result] The leaf stalk of A.andraeanum was the optimum explant,with the highest callus induction rate（78.3%）.The best disinfecting effect could be obtained when the explants was sterilized with 0.1% HgCl2 for 8 min.The optimum media for the callus induction of Ping Champion and MIDORI were respectively 1/2 MS＋1.50 mg/L 6-BA＋0.50 mg/L 2,4-D＋0.10 mg/L NAA and 1/2 MS＋1.50 mg/L 6-BA＋1.00 mg/L KT＋0.10 mg/L 2,4-D,while the optimum media for callus subculture of Ping Champion and MIDORI were respectively MS＋1.00 mg/L 6-BA＋0.50 mg/L NAA＋0.30 mg/L 2,4-D and MS＋0.50 mg/L 6-BA＋0.50 mg/L KT＋0.10 mg/L NAA.It was beneficial for the proliferation and growth of cluster buds while 10%（w/v）of coconut water or banana jam was added to MS medium at the proliferation stage,and the proliferation rate could be increased by 2.83 fold.At the rooting stage,the medium 1/2 MS＋0.10 mg/L NAA＋0.10 mg/L IAA was the optimum medium for rooting,and the rooting rate was up to 100%.[Conservation] The research results will lay a foundation for the establishment of the industrial seedling breeding system of A.andraeanum,and it will be significant for the development of A.andraeanum industry.
基金supported by the Natural Science Foundation of Guangdong Province (05300848)Fok Ying Tung Education Foundation (104031)the National Natural Science Foundation of China(30800758)
文摘This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.
基金Supported by Special Foundation of President of Guangdong Academy of Agricultural Sciences(201217)
文摘At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products have significantly affected human life. Anthurium andraeanum is the second major tropical potted flower and its transgenic breeding has a promising prospect of application. In this paper, acceptors, transformation methods and introduced exogenous genes （ including reporter genes, selectable marker genes and target genes） of Anthurium andraeanum were summarized; in addition, several issues related to transforma- tion of Anthurium andraeanum were analyzed, aiming at providing reference for transgenic breeding of Anthurium andraeanum.
基金Supported by Suzhou Agricultural Applied Basic Research Program(SNG201605)。
文摘[Objectives] This study was conducted to explore the color variation mechanism of Anthurium andraeanum spathe at the protein level. [Methods]The differential proteins of wild type and its white mutant were separated and identified by using one-dimensional gel electrophoresis and mass spectrometry( 1-DE/MS). [Results] Compared with leaves and spadices,the 1-DE patterns of two kinds of spathe proteins were significantly different,and two different bands were detected in wild type spathes and mutant spathes respectively. The four significantly differential bands were selected and analyzed by mass spectrometry,and 138,111,70 and 427 proteins were identified respectively. The results of GO functional annotation analysis showed that the molecular functions of the proteins were mainly catalytic activity and binding,and the main biological processes involved were cellular process and metabolic process. Many proteins involved in the synthesis of anthocyanins and flavonoids,sugar metabolism and some resistance proteins were screened,indicating that the spathe color difference of A. andraeanum‘Pink champion'is not only related to anthocyanin anabolism,but also regulated by various metabolic pathways. [Conclusions]The study provides a new experimental basis for elucidating the molecular mechanism of the regulation of A. andraeanum flower color.
基金Supported by the Applied Basic Research Program of Suzhou(SNG201605)Kunshan Ecological Agricultural Science and Technology Project(KN1614)
文摘In order to understand the mechanism of spathe color variation in Anthurium andraeanum at the protein level, the leaves, inflorescences and spathes of the wild type and two mutants of A. andraeanum 'Madural' were used as research objects in which the differential expression of proteins related to flower color mutants was analyzed by one-dimensional electrophoresis and mass spectrometry(1-DE/MS). The 1-DE patterns showed that the protein components expressed highly in spathes were mainly concentrated in the molecular weight range of 20-42 kD, and differential bands were detected between the wild type and the mutant, while no significant differences were detected in the leaf and inflorescence proteins. According to the results of mass spectrometry analysis of the differential bands, 21 known functional proteins involved in life processes such as glucose metabolism, resistance, cytoskeleton, gene regulation and signal transduction were identified. It showed that in addition to the influences from anthocyanidins, the spathe color variation of A. andraeanum 'Madural' is also regulated by a variety of metabolic pathway-related proteins.
基金supported by the Science and Technology Key Project ofZhejiang Province(No.2009C12095)the National NaturalScience Foundation of China(No.31200527)
文摘An 888-bp ful-length ascorbate peroxidase （APX） complementary DNA （cDNA） gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame （ORF）. This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting ofα-helixes,β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction （RT-PCR） analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage （EL） and malondialdehyde （MDA） content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cellmembrane damage and ultimately enhance the plant cold tolerance.
