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Anti-Fatigue Effect of Blended <i>Camellia oleifera Abel</i>Tea Oil and Ge-132 in Mice 被引量:5
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作者 Lisong Hu Xuezhi Fang +1 位作者 Menghao Du Jingping Zhang 《Food and Nutrition Sciences》 2015年第15期1479-1487,共9页
Nowadays, people are busier and busier for working and living, and suffer a lot of pressure on their body or mind. Therefore, people are prone to have fatigue activity and decrease their working efficiency and happine... Nowadays, people are busier and busier for working and living, and suffer a lot of pressure on their body or mind. Therefore, people are prone to have fatigue activity and decrease their working efficiency and happiness. It was reported that fatigue is a common symptom in the community, with up to half of the general population complaining of fatigue. More and more researchers devoted themselves to studying natural active ingredients in organism as the anti-fatigue drugs to release fatigue symptom. However, these natural ingredients were difficult to obtain from plants, animals and microorganisms by separating and purifying. In addition, some active substances have many side effects. In our study, we employed tea seed oil as main ingredients blended with bis-(carboxyethylgermanium) sesquioxide (Ge-132) to investigate the effects of anti-fatigue on mice by administrating mice with low dose, intermediate dose and high dose of tea seed oil complex for 0, 2 or 4 weeks. The specific tests of studying effects of anti-fatigue were body weight, weight-loaded force swimming, blood urea nitrogen, blood lactic acid and hepaticglycogen. And the results showed that appropriate level of tea seed oil complex could decrease the body weight and prolong the weight-loaded swimming time, and had an active effect on the bloodurea nitrogen, hepatic glycogen and blood lactic acid level mice, which significantly embodied the anti-fatigue activity of tea seed oil complex. 展开更多
关键词 TEA Seed OIL GE-132 anti-fatigue Blending
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Present Situation of the Anti-Fatigue Processing of High-Strength Steel Internal Thread Based on Cold Extrusion Technology:A Review 被引量:5
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作者 Hong MIAO Cheng JIANG +2 位作者 Sixing LIU Shanwen ZHANG Yanjun ZHANG 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2017年第2期231-240,共10页
The adoption of cold-extrusion forming for internal thread net forming becomes an important component of anti-fatigue processing with the development of internal thread processing towards high performance, low cost an... The adoption of cold-extrusion forming for internal thread net forming becomes an important component of anti-fatigue processing with the development of internal thread processing towards high performance, low cost and low energy consumption. It has vast application foreground in the field of aviation, spaceflight, high speed train and etc. The internal thread processing and anti-fatigue manufacture technology are summarized. In terms of the perspective of processing quality and fatigue serving life, the advantages and disadvantages of the processing methods from are compared. The internal thread cold-extrusion processing technology is investigated for the purpose of improving the anti-fatigue serving life of internal thread. The superiorities of the plastic deformation law and surface integrity of the metal layer in the course of cold extrusion for improving its stability and economy are summed up. The proposed research forecasts the develop- ment tendency of the internal thread anti-fatigue manufacturing technology. 展开更多
关键词 High-strength steel · Internal thread · Coldextrusion · anti-fatigue · Surface integrity
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基于肠道菌群-短链脂肪酸途径的人参改善运动性疲劳作用机制探索
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作者 刘晶晶 金红宇 +5 位作者 胡晓茹 刘静 汪祺 徐蓓蕾 左甜甜 魏锋 《中国现代中药》 CAS 2024年第11期1930-1937,共8页
目的:探索人参提取物通过调节肠道菌群发挥抗疲劳作用的机制。方法:制备大鼠负重游泳运动性疲劳模型,连续灌胃给予人参提取物21 d。采用负重游泳实验评价人参提取物抗疲劳作用;收集大鼠血清、肠道内容物及各组织,采用紫外-可见分光光度... 目的:探索人参提取物通过调节肠道菌群发挥抗疲劳作用的机制。方法:制备大鼠负重游泳运动性疲劳模型,连续灌胃给予人参提取物21 d。采用负重游泳实验评价人参提取物抗疲劳作用;收集大鼠血清、肠道内容物及各组织,采用紫外-可见分光光度法测定人参提取物中总皂苷和总多糖含量;采用比色法检测尿素氮、乳酸和肝/肌糖原水平;采用酶联免疫吸附法检测白细胞介素-6 (IL-6)、IL-1β和C反应蛋白(CRP)水平;采用16S rRNA结合多元统计分析探索肠道菌群变化;采用液相色谱-质谱法(LC-MS/MS)测定血清短链脂肪酸变化。结果:人参提取物中总皂苷和总多糖质量分数分别为205.6、321.8 mg·g~(–1)。与模型组比较,人参提取物给药后能够显著延长大鼠负重游泳时间,同时上调肝脏和骨骼肌组织中糖原水平,下调血清中尿素氮和乳酸水平。与模型组比较,人参提取物可促进生成短链脂肪酸的肠道菌群表达,并提高血清中短链脂肪酸水平,同时下调炎症因子水平。结论:人参提取物具有明显的抗疲劳作用,其机制可能与调控肠道菌群促进短链脂肪酸生成,进而促进糖原生成,同时抑制炎症因子表达有关。 展开更多
关键词 人参 抗疲劳 肠道菌群 短链脂肪酸 炎症因子
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基于HPLC-Q-TOF MS/MS和网络药理学的参蓉蛹草片质量标志物研究 被引量:1
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作者 于鑫畅 韩彦琪 +4 位作者 郝艳琦 刘建庭 许浚 蒋庆峰 张铁军 《中国现代中药》 CAS 2024年第2期289-307,共19页
目的:应用高效液相色谱-四极杆飞行时间质谱法(HPLC-Q-TOF-MS/MS)建立参蓉蛹草片化学成分的检测方法,并结合网络药理学和分子对接方法分析其抗疲劳的网络调控机制、预测参蓉蛹草片的质量标志物。方法:运用HPLC-Q-TOF-MS/MS对参蓉蛹草片... 目的:应用高效液相色谱-四极杆飞行时间质谱法(HPLC-Q-TOF-MS/MS)建立参蓉蛹草片化学成分的检测方法,并结合网络药理学和分子对接方法分析其抗疲劳的网络调控机制、预测参蓉蛹草片的质量标志物。方法:运用HPLC-Q-TOF-MS/MS对参蓉蛹草片物质基础进行分析鉴定。通过PubChem获取所选化合物的结构,利用TCMSP数据库及SwissTargetPrediction获取作用靶点;通过GeneCards、TTD、OMIM数据库筛选与疲劳相关的疾病靶点,获得药物和疲劳的交集靶点;运用UniProt数据库校准靶点名称;利用STRING数据库构建交集基因蛋白质-蛋白质相互作用(PPI)网络,并运用Cytoscape 3.8. 0软件对PPI网络进行拓扑分析;最后借助Metascape在线分析核心交集基因,进行基因本体(GO)功能富集分析、京都基因与基因组百科全书(KEGG)通路富集分析,并对分析结果进行可视化。结果:通过HPLC-Q-TOF MS/MS共得到参蓉蛹草片124个化学成分,利用网络药理学对筛选出的23个化学成分进行生物信息学分析,发现其可作用于195个靶点干预33条信号通路。分子对接结果显示,活性化合物可进入受体靶点活性空腔,与其形成的对接构象合理。结论:参蓉蛹草片可通过多成分、多靶点、多途径发挥抗疲劳作用,基于HPLC-Q-TOF MS/MS,结合网络药理学和分子对接方法初步将人参皂苷类、苯乙醇苷类预测为参蓉蛹草片的质量标志物。 展开更多
关键词 参蓉蛹草片 高效液相色谱-四极杆飞行时间质谱法 网络药理学 质量标志物 抗疲劳
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A two-parameter model to predict fatigue life of high-strength steels in a very high cycle fatigue regime 被引量:7
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作者 Chengqi Sun Xiaolong Liu Youshi Hong 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2015年第3期383-391,共9页
In this paper, ultrasonic (20 kHz) fatigue tests were performed on specimens of a high-strength steel in very high cycle fatigue (VHCF) regime. Experimental results showed that for most tested specimens failed in ... In this paper, ultrasonic (20 kHz) fatigue tests were performed on specimens of a high-strength steel in very high cycle fatigue (VHCF) regime. Experimental results showed that for most tested specimens failed in a VHCF regime, a fatigue crack originated from the interior of specimen with a fish-eye pattern, which contained a fine granular area (FGA) centered by an inclusion as the crack origin. Then, a two-parameter model is proposed to predict the fatigue life of high-strength steels with fish-eye mode failure in a VHCF regime, which takes into account the inclusion size and the FGA size. The model was verified by the data of present experiments and those in the literature. Furthermore, an analytic formula was obtained for estimating the equivalent crack growth rate within the FGA. The results also indicated that the stress intensity factor range at the front of the FGA varies within a small range, which is irrespective of stress amplitude and fatigue life. 展开更多
关键词 Very high-cycle fatigue - High-strength steels fatigue life Inclusion size Crack growth rate
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EFFECT OF DYNAMIC STRAIN AGING ON LOW CYCLE FATIGUE BEHAVIOUR OF 18-8 AUSTENITIC STAINLESS STEEL 被引量:2
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作者 PENG Kaiping CHEN Wenzhe OIAN Kuangwu Fuzhou University,Fuzhou,China teaching assistant,Department of Mechanical Engineering,Fuzhou University,Fuzhou 350002,China 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 1993年第6期420-425,共6页
Studies were made of the symmetric tensile-compressive low cycle fatigue behaviour and the influence of dynamic strain aging(DSA)pre-treatment of 18-8 austenitic stainless steel. Within the testing amplitude range of ... Studies were made of the symmetric tensile-compressive low cycle fatigue behaviour and the influence of dynamic strain aging(DSA)pre-treatment of 18-8 austenitic stainless steel. Within the testing amplitude range of strain.±0.5 % to±1.5 %,the three processes of cyclic hardening,cyclic saturation and cyclic softening were observed.In the same amplitude of strain,the peak stress of the samples pre-treated by DSA is higher than that of solid-solu- tion and cold working pre-treatment,but no remarkable differences of the fatigue lives of them were found.TEM observation shows that the uniform and stable dislocation networks with high density form after DSA pre-treatment,which increases the cyclic peak stress.The cyclic softening results from the low dislocation density and elongated cell structure with low energy. 展开更多
关键词 18-8 austenitic stainless steel dynamic strain aging low cycle fatigue
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Bi-variable damage model for fatigue life prediction of metal components 被引量:1
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作者 Miao Zhang Qing-Chun Meng Xing Zhang Wei-Ping Hu 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2011年第3期416-425,共10页
Based on the theory of continuum damage mechanics,a bi-variable damage mechanics model is developed,which,according to thermodynamics,is accessible to derivation of damage driving force,damage evolution equation and d... Based on the theory of continuum damage mechanics,a bi-variable damage mechanics model is developed,which,according to thermodynamics,is accessible to derivation of damage driving force,damage evolution equation and damage evolution criteria. Furthermore,damage evolution equations of time rate are established by the generalized Drucker's postulate. The damage evolution equation of cycle rate is obtained by integrating the time damage evolution equations,and the fatigue life prediction method for smooth specimens under repeated loading with constant strain amplitude is constructed. Likewise,for notched specimens under the repeated loading with constant strain amplitude,the fatigue life prediction method is obtained on the ground of the theory of conservative integral in damage mechanics. Thus,the material parameters in the damage evolution equation can be obtained by reference to the fatigue test results of standard specimens with stress concentration factor equal to 1,2 and 3. 展开更多
关键词 Bi-variable damage model - Damage evolution equation . Life prediction - fatigue . Damage mechanics
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基于星点设计-响应面法研制黄精益智仁复合固体饮料及抗疲劳作用研究
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作者 张瑞博 闫小娟 张海悦 《中国食品添加剂》 CAS 2024年第11期153-161,共9页
以黄精、益智仁、茯苓、芡实、枸杞、山药、大枣、山楂等8味药食同源地道药材,开发一款具有抗疲劳功能的黄精益智仁固体饮料(PASB)。利用单因素以及星点设计-响应面试验优化其煎煮提取工艺,喷雾干燥制得的固体饮料,并通过小鼠负重游泳... 以黄精、益智仁、茯苓、芡实、枸杞、山药、大枣、山楂等8味药食同源地道药材,开发一款具有抗疲劳功能的黄精益智仁固体饮料(PASB)。利用单因素以及星点设计-响应面试验优化其煎煮提取工艺,喷雾干燥制得的固体饮料,并通过小鼠负重游泳试验考察抗疲劳效果。结果表明最佳煎煮工艺为:料液比1∶13 g/mL,煎煮时间100 min,浸泡60 min,煎煮两次。同时动物实验结果显示,PASB能够延长小鼠负重游泳时间,降低血乳酸、血清尿素氮的堆积,提高肝糖原含量,表明黄精益智仁固体饮料具有较好的抗疲劳活性。 展开更多
关键词 黄精益智仁固体饮料 星点设计-响应面 负重游泳实验 抗疲劳
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基于AMPK/PGC-1α信号通路探讨菟丝子多糖改善大鼠运动能力的作用及机制
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作者 庞伊婷 麻飞 《云南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期53-59,共7页
【目的】基于腺苷酸活化蛋白激酶(AMPK)/过氧化物酶体增殖活化受体γ共激活因子1α(PGC-1α)信号通路探讨菟丝子多糖改善大鼠运动能力的作用及潜在机制。【方法】将大鼠50只分为正常对照组、运动模型组及菟丝子多糖低、中、高剂量组(10... 【目的】基于腺苷酸活化蛋白激酶(AMPK)/过氧化物酶体增殖活化受体γ共激活因子1α(PGC-1α)信号通路探讨菟丝子多糖改善大鼠运动能力的作用及潜在机制。【方法】将大鼠50只分为正常对照组、运动模型组及菟丝子多糖低、中、高剂量组(10、200和400 mg/kg),除正常对照组外,其余各组大鼠进行游泳训练。游泳至力竭后,测定各组大鼠血清抗疲劳指标以及肝组织能量代谢和氧化应激指标,AMPKα和PGC-1αmRNA的表达水平,磷酸化AMPKα(p-AMPKα)、AMPKα和PGC-1α的蛋白表达水平。【结果】与运动模型组相比,菟丝子多糖低、中、高剂量组大鼠游泳力竭时间显著或极显著延长,血清尿素氮、乳酸、皮质酮以及肝组织丙二醛含量极显著减少,血清睾酮以及肝组织糖原、钠钾ATP酶、钙镁ATP酶、超氧化物歧化酶、谷胱甘肽过氧化物酶水平显著增加,肝组织AMPKαmRNA及其蛋白表达无明显变化,PGC-1αmRNA及p-AMPKα、PGC-1α蛋白表达极显著增加。【结论】菟丝子多糖可提升高强度大运动量训练大鼠的运动能力,且该作用与抗疲劳、增加能量物质储备、抑制氧化应激及活化AMPK/PGC-1α信号通路等有关。 展开更多
关键词 菟丝子多糖 运动能力 抗疲劳 氧化应激 AMPK/PGC-1α信号通路
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正交设计与Box-Behnken响应面法优化当归多糖抗疲劳饮料制备工艺
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作者 张宇轩 李勃深 +1 位作者 李晓琳 李茂星 《解放军药学学报》 CAS 2024年第4期348-354,共7页
目的比较不同原料配方对当归多糖抗疲劳饮料感官指标的影响、不同复合稳定剂配方对当归多糖抗疲劳饮料稳定性的影响,分别利用正交设计与Box-Behnken响应面法优化当归多糖抗疲劳饮料的原料配方及稳定剂配方。方法以当归多糖为原料,研制... 目的比较不同原料配方对当归多糖抗疲劳饮料感官指标的影响、不同复合稳定剂配方对当归多糖抗疲劳饮料稳定性的影响,分别利用正交设计与Box-Behnken响应面法优化当归多糖抗疲劳饮料的原料配方及稳定剂配方。方法以当归多糖为原料,研制当归多糖抗疲劳饮料。利用单因素实验和正交试验,考察当归多糖、甜菊糖苷、柠檬酸添加量的原料配比对当归多糖抗疲劳饮料感官指标的影响;采用Box-Behnken响应面优化果胶、海藻酸钠、羧甲基纤维素钠添加量的复合稳定剂对当归多糖抗疲劳饮料稳定性的影响。最终确定该饮料的优选配方参数。结果在当归多糖添加量10.0%,甜菊糖苷添加量0.035%,柠檬酸添加量0.20%时获得了感官评分为(66.333±3.055)分的饮料原料;在此基础上果胶添加量0.015%,海藻酸钠添加量0.080%,羧甲基纤维素钠添加量0.20%时获得了沉淀率预测值为2.272%,实际值为(2.339±0.111)%的当归多糖抗疲劳饮料。结论回归模型的预测值与实测值接近,采用单因素实验、正交试验和响应面法共同优化的当归多糖抗疲劳饮料工艺合理可行,并成功制备出了棕色、澄清、酸甜适宜的当归多糖抗疲劳饮料。 展开更多
关键词 当归多糖 抗疲劳饮料 正交设计 BOX-BEHNKEN设计
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The Influence of Ceramic Coating on Fatigue Crack Growth Behaviour of X17CrNi15-2 Stainless Steel
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作者 Ran Li Wenshu Wei +13 位作者 Shoubin Li Rui Zhao Hao Liu Jiankai Zhang Jian Ye Huigang Wu Yao Xu Fei Xiao Mengyu Wu Dalong Wang Jingbin Chen Wei Wang Na Gao Rongming Chen 《Engineering(科研)》 2020年第2期140-149,共10页
Fatigue crack growth tests were carried out on the SEN B3 precracked specimens, with dimensions in accordance with ISO 12108 requirements. The specimens were made of martensitic stainless steel, X17CrNi15-2, and some ... Fatigue crack growth tests were carried out on the SEN B3 precracked specimens, with dimensions in accordance with ISO 12108 requirements. The specimens were made of martensitic stainless steel, X17CrNi15-2, and some of them were modified by the ceramic coating deposition surface treatment. The effects of ceramic coating, on the fatigue crack growth behaviour of hollow shaft specimens, were experimentally investigated. Fatigue crack growth rates, da/dN, were characterised, using the power law relationship between da/dN (in mm/cycle) and the stress intensity factor range, ΔK (in MPa&#8729;m0.5). The two constants of the correlation are 7.9768 × 10&#8722;9 and 2.8107 for the parent material, and those for the coated material are 2.4391 × 10&#8722;9 and 3.1990, respectively. Microstructural analyses were carried out on the tested specimens, which shows that the maximum hardness of the ceramic coating is higher than that of substrate by a factor of ~3.2. The dimple fracture dominates the final fracture mechanism for the parent material, and the combination of fatigue, ductile fracture and cleavage dominates the final fracture mechanism for the coated material, based on the SEM analyses. EDS tests’ results reveal that the parent material specimen shows higher levels of C at matrix regions along with Fe- and O-rich regions, compared with the coated material specimen. 展开更多
关键词 fatigue CRACK Growth SEN B3 Precracked SPECIMEN X17CrNi15-2 CERAMIC Coating DEPOSITION Surface Treatment Corrosion Resistance
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A Comparative Study of Antifatigue Effects of Taurine and Vitamin C on Chronic Fatigue Syndrome
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作者 Shin-Hee Kim Hyun-Jin Kim +4 位作者 Semi Kim Ju-Seop Kang Young Tae Koo Sang Hun Lee Dong-Hyun Paik 《Pharmacology & Pharmacy》 CAS 2022年第8期300-312,共13页
Vitamin C and taurine (TR) are well known as active components for fatigue recovery. However, the mechanism of the anti-fatigue effects of vitamin C and TR is still unclear. Our study was designed to evaluate the anti... Vitamin C and taurine (TR) are well known as active components for fatigue recovery. However, the mechanism of the anti-fatigue effects of vitamin C and TR is still unclear. Our study was designed to evaluate the anti-fatigue activities of vitamin C and TR in an animal test for fatigue and to compare the activities between vitamin C and TR. Materials and Methods: Vitamin C, TR or their combination were orally administrated to mice once daily for 15 days, and then metabolic activities such as blood glucose, triglyceride (TG), lactate, and lactate dehydrogenase (LDH) as well as antioxidant activities such as malondialdehyde (MDA) and superoxide dismutase (SOD) were determined (evaluated) after forced swimming test (FST). Results: Compared with the control group, C100, C200, and T50 showed a tendency to decrease mobility in FST. Moreover, TG (C100, C200, T200), LDH (C200), lactic acid (C100) and MDA (C50, C100, C200) levels were inhibited by vitamin C and TR. Conclusions: These results suggest that vitamin C and TR have anti-fatigue activities in mice, with vitamin C providing a stronger effect. 展开更多
关键词 Vitamin C TAURINE Chronic fatigue Syndrome anti-fatigue Effect
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疲劳症状管理方案在肺癌抗PD-1抗体免疫治疗期间的应用效果
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作者 陈磊 《全科护理》 2024年第7期1293-1297,共5页
目的:探讨疲劳症状管理方案在肺癌抗PD-1抗体免疫治疗期间中的应用效果。方法:选取医院肺癌抗PD-1抗体免疫治疗病人94例为研究对象,应用随机数字表法将病人分为观察组及对照组各47例。对照组治疗期间行常规护理,观察组实施疲劳症状管理... 目的:探讨疲劳症状管理方案在肺癌抗PD-1抗体免疫治疗期间中的应用效果。方法:选取医院肺癌抗PD-1抗体免疫治疗病人94例为研究对象,应用随机数字表法将病人分为观察组及对照组各47例。对照组治疗期间行常规护理,观察组实施疲劳症状管理方案,比较两组干预前后癌因性疲乏感、症状、心理弹性及生活质量。结果:干预后观察组癌因性疲乏感评分及安德森症状评估量表(MDASI)评分明显低于对照组(P<0.05),而观察组干预后心理弹性评分及肺癌治疗功能性量表(FACT-L)相关维度评分较对照组明显提升(P<0.05)。结论:疲劳症状管理方案能有效减轻肺癌抗PD-1抗体免疫治疗期间癌因性疲乏感及疾病不确定感,可提升病人心理弹性水平,改善病人生活质量。 展开更多
关键词 疲劳症状管理方案 肺癌 抗PD-1抗体免疫治疗 生活质量
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基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究 被引量:2
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作者 赖丽燕 刘峥 +2 位作者 李巍 周昱 黄红霞 《分析科学学报》 CAS CSCD 北大核心 2014年第4期481-484,共4页
应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH... 应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限(S/N=3)为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。 展开更多
关键词 氧化石墨烯 3 5-二溴水杨醛席夫碱镍配合物 anti—IgG 电化学免疫传感器
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信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨 被引量:1
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作者 文海平 周红 +4 位作者 许国莹 郭东琳 周芳 陈东东 解鸿翔 《中国免疫学杂志》 CAS CSCD 北大核心 2011年第4期295-299,共5页
目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合... 目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。 展开更多
关键词 抗磷脂综合征 antiβ-2GPI/β2GPI IRAK1 IRAK4 组织因子
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基于抗疲劳性能的ATB-25油石比设计
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作者 田小革 郭常辉 +2 位作者 李光耀 胡宏立 卢雪蓉 《交通科学与工程》 2023年第6期48-56,共9页
为确定符合抗疲劳性能要求的橡胶沥青ATB-25油石比范围,通过沥青常规试验和动态剪切流变试验(DSR)确定最佳胶粉掺量,以优化的主骨架空隙填充法进行橡胶沥青ATB-25配合比设计,并确定初始油石比。制备橡胶沥青ATB-25的小梁试件,开展四点... 为确定符合抗疲劳性能要求的橡胶沥青ATB-25油石比范围,通过沥青常规试验和动态剪切流变试验(DSR)确定最佳胶粉掺量,以优化的主骨架空隙填充法进行橡胶沥青ATB-25配合比设计,并确定初始油石比。制备橡胶沥青ATB-25的小梁试件,开展四点弯曲疲劳试验,建立5种油石比下的疲劳寿命方程。结合湖南某高速公路橡胶沥青ATB-25的基层所受弯拉应力,得到该应力水平下橡胶沥青ATB-25的弯拉疲劳寿命与油石比的关系曲线。以同时满足橡胶沥青ATB-25弯拉疲劳寿命和剪切疲劳寿命的要求为指标,确定符合抗疲劳性能的油石比范围为4.0%~4.4%。研究结果表明:根据该油石比优化方法,设计的橡胶沥青ATB-25的抗疲劳性能可满足要求,且具有良好的高温稳定性。 展开更多
关键词 橡胶沥青ATB-25 动态剪切流变试验 主骨架空隙填充法 疲劳试验 油石比优化 抗疲劳性能
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三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类
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作者 于海鸥 高瑞梅 +1 位作者 孔令令 裴东河 《东北师大学报(自然科学版)》 CAS CSCD 北大核心 2010年第4期35-45,共11页
利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kr... 利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质. 展开更多
关键词 Lorentzian曲面 三维anti DE SITTER空间 St1×Ss1-值光锥Gauss映射 Lorentzian光维高度函数
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至宝三鞭丸基于Nrf2/HO-1通路的抗疲劳作用机制研究 被引量:2
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作者 杨坤 王敬博 +7 位作者 陶雪莹 康世奇 陈畅 苏晓慧 杜茂波 王官连 张咏梅 孔祥英 《中药新药与临床药理》 CAS CSCD 北大核心 2023年第3期335-340,共6页
目的通过观察至宝三鞭丸(Zhibao Sanbian Pills)对Nrf2/HO-1信号通路的影响,研究其对小鼠的抗疲劳作用及机制。方法将ICR小鼠随机分为正常对照组、至宝三鞭丸低剂量(0.958 g·kg^(-1))组、至宝三鞭丸中剂量(4.79 g·kg^(-1))组... 目的通过观察至宝三鞭丸(Zhibao Sanbian Pills)对Nrf2/HO-1信号通路的影响,研究其对小鼠的抗疲劳作用及机制。方法将ICR小鼠随机分为正常对照组、至宝三鞭丸低剂量(0.958 g·kg^(-1))组、至宝三鞭丸中剂量(4.79 g·kg^(-1))组、至宝三鞭丸高剂量(9.58 g·kg^(-1))组,每组25只,连续给药35 d,记录体质量。采用负重游泳实验评价至宝三鞭丸的抗疲劳作用;比色法检测各组小鼠血清中的尿素氮(Urea nitrogen,UN)和乳酸(Lactic acid)水平;比色法检测各组小鼠肝组织中的糖原(Liver glycogen)含量;Western Blot检测各组小鼠肝组织中核因子红细胞2相关因子2(Nuclear factor erythroid 2-related factor 2,Nrf2)、核孔糖蛋白62(Sequestosome 1,P62/SQSTM1)、血红素加氧酶1(Heme oxygenase-1,HO-1)和醌氧化还原酶1(Quinone oxidoreductase 1,NQO1)的蛋白表达水平。结果与正常对照组比,至宝三鞭丸各剂量组可不同程度延长小鼠负重游泳时间,提高肝脏组织中糖原含量,下调血清中尿素氮水平,其中至宝三鞭丸高剂量(9.58 g·kg^(-1))组的差异具有统计学意义(均P<0.05);至宝三鞭丸虽具有降低乳酸堆积的作用趋势,但差异无统计学意义(P>0.05);此外,至宝三鞭丸各剂量组均可提高肝组织中Nrf2、P62、HO-1和NQO1的蛋白表达水平(P<0.05,P<0.01)。结论至宝三鞭丸具有明显的抗疲劳作用,其机制可能与激活Nrf2/HO-1信号通路,从而提高机体的抗氧化能力有关。 展开更多
关键词 至宝三鞭丸 抗疲劳 氧化应激 Nrf2/HO-1信号通路 小鼠
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富含3,5-二羟基-4-甲氧基苯甲醇牡蛎肽制备及其抗疲劳功效研究 被引量:1
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作者 郑玉婷 于雁飞 +5 位作者 刘晔 李若姝 冯婷玉 王玉明 薛长湖 张恬恬 《食品安全质量检测学报》 CAS 北大核心 2023年第21期173-182,共10页
目的研究富含3,5-二羟基-4-甲氧基苯甲醇(3,5-dihydroxy-4-methoxybenzyl alcohol,DHMBA)牡蛎肽制备工艺及其抗疲劳功效。方法通过响应面优化设计,考察牡蛎原料预处理工艺中乳酸菌添加量、酵母菌添加量和蒸煮时间对牡蛎肽中DHMBA含量的... 目的研究富含3,5-二羟基-4-甲氧基苯甲醇(3,5-dihydroxy-4-methoxybenzyl alcohol,DHMBA)牡蛎肽制备工艺及其抗疲劳功效。方法通过响应面优化设计,考察牡蛎原料预处理工艺中乳酸菌添加量、酵母菌添加量和蒸煮时间对牡蛎肽中DHMBA含量的影响。同时,通过正常及慢性疲劳模型小鼠的力竭跑步或力竭爬杆实验,探讨富含DHMBA牡蛎肽的抗疲劳作用。结果富含DHMBA牡蛎肽的最佳原料预处理工艺为:乳酸菌添加量为1.50%、酵母菌添加量为1.00%、蒸煮时间为7 h;20 mg/(kg·bw)富含DHMBA的牡蛎肽可显著延长小鼠的力竭跑步或爬杆时长,提高机体运动负荷能力,且使皮质酮所致慢性疲劳模型小鼠跑步时间延长了76.02%;DHMBA与不含DHMBA牡蛎肽复配组抗疲劳功效显著优于不含DHMBA牡蛎肽组,DHMBA能够有效提升牡蛎肽的抗疲劳功效。结论富含DHMBA的牡蛎肽具有抗疲劳作用,且这种抗疲劳的显著功效可能与牡蛎肽中富含的DHMBA有关,富含DHMBA牡蛎肽的原料预处理工艺的优化为进一步开发利用牡蛎肽资源提供了理论依据。 展开更多
关键词 牡蛎肽 3 5-二羟基-4-甲氧基苯甲醇 工艺优化 抗疲劳
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乳酸菌发酵对青稞β-葡聚糖加工特性及其耐缺氧和抗疲劳作用影响 被引量:3
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作者 姜敬维 李玟君 +4 位作者 李建湘 宋青云 李玉蝶 汪海燕 李玮 《食品研究与开发》 CAS 北大核心 2023年第14期30-35,共6页
该文研究植物乳杆菌(Lactobacillus plantarum,LP)、保加利亚乳杆菌(Lactobacillus bulgaricus,LB)、鼠李糖乳杆菌(Lactobacillus rhamnosus,LR)发酵对青稞β-葡聚糖加工特性及其耐缺氧、抗疲劳作用的影响。结果表明,LB发酵制备青稞β-... 该文研究植物乳杆菌(Lactobacillus plantarum,LP)、保加利亚乳杆菌(Lactobacillus bulgaricus,LB)、鼠李糖乳杆菌(Lactobacillus rhamnosus,LR)发酵对青稞β-葡聚糖加工特性及其耐缺氧、抗疲劳作用的影响。结果表明,LB发酵制备青稞β-葡聚糖的含量高于LP和LR,达到(5.14±0.22)%。保加利亚乳杆菌发酵后得到的β-葡聚糖(Lactobacillus bulgaricus β-glucan,LBG)中多糖均一性较高,起泡能力和溶解性均显著高于植物乳杆菌发酵后得到的β-葡聚糖(Lactobacillus plantarum β-glucan,LPG)、鼠李糖发酵后得到的β-葡聚糖(Lactobacillus rhamnosus β-glucan,LRG)和未发酵得到的β-葡聚糖(no fermentation β-glucan,NFG)起泡稳定性显著高于LPG、LBG和NFG。LPG、LBG、LRG与NFG相比,密闭缺氧存活时间延长了26.6%、42.3%和36.4%,游泳存活时间延长了22.6%、40.3%和42.4%;生理指标检测发现,添加LBG的小鼠乳酸脱氢酶(lactate dehydrogenase,LDH)含量均高于LPG和LRG组,是CK和NFG的2.07倍和1.38倍,且显著降低小鼠体内丙二醛(malondialdehyde,MDA)、提升(superoxide dismutase,SOD)的含量;而LRG能显著降低小鼠的血尿素氮(blood urea nitrogen,BUN)和乳酸(lactic acid,LA),且肝糖原的含量远高于CK、NFG和LBG。因此,LP、LB、LR均能有效改善青稞β-葡聚糖对小鼠耐缺氧和抗疲劳的效果,LP、LB和LR提升β-葡聚糖抗疲劳效果无显著性差异;LP和LR提升β-葡聚糖耐缺氧效果无显著性差异。 展开更多
关键词 乳酸菌 青稞Β-葡聚糖 加工特性 耐缺氧 抗疲劳
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