目的探讨硫化铜(CuS)/氧化石墨烯(GO)/壳聚糖(CS)/纳米羟基磷灰石(nHA)复合材料(CGCHs)的抗菌和促成骨作用及其作用机制。方法采用水热法合成CuS/GO纳米颗粒,通过原位沉淀法合成CS/nHA支架和CGCHs支架,检测材料表征、光热转换性能和生...目的探讨硫化铜(CuS)/氧化石墨烯(GO)/壳聚糖(CS)/纳米羟基磷灰石(nHA)复合材料(CGCHs)的抗菌和促成骨作用及其作用机制。方法采用水热法合成CuS/GO纳米颗粒,通过原位沉淀法合成CS/nHA支架和CGCHs支架,检测材料表征、光热转换性能和生物安全性,评估CGCHs组和近红外光(NIR)照射下CGCHs(CGCHs+NIR)组的细菌抑制效果及其对细菌生物膜相关基因表达的影响,观察CGCHs和CS/nHA不同材料组的促成骨分化和成骨、破骨相关基因表达。结果CGCHs是具有高度孔隙率的三维支架,在CuS/GO浓度为200μg/mL时CGCHs同时兼具良好的红外升温效果和生物安全性。琼脂糖平板涂菌和细菌死活染色结果均表明CGCHs+NIR组抗菌性能最佳,生物膜相关基因qPCR检测证实其具有抑制细菌生物膜相关基因表达的作用。茜素红染色结果表明CGCHs具有良好的体外促成骨性能,体外共培养3、7、14、21和28 d qPCR结果表明CGCHs对成骨早期和晚期相关基因表达均具有促进作用。与破骨细胞共培养结果可观察到CGCHs具有抑制破骨细胞形成的作用,细胞凋亡检测结果进一步验证这一结论,破骨分化相关基因qPCR检测结果表明,CGCHs主要通过抑制抗酒石酸酸性磷酸酶、组织蛋白酶K、CTR、P65和P38在共培养7、14 d的表达来抑制破骨细胞的分化。结论作为纳米复合材料,CGCHs生物安全性好,具有良好的红外光热协同抗菌作用,在促成骨分化的同时抑制破骨细胞分化,有望为感染性骨缺损治疗提供新的思路。展开更多
Detection of liquid mixture of different volume ratio is very important in industrial purposes. The paper reports a sensing mechanism of binary liquid mixture for different volume fraction, based on the measurement of...Detection of liquid mixture of different volume ratio is very important in industrial purposes. The paper reports a sensing mechanism of binary liquid mixture for different volume fraction, based on the measurement of refractive index of the mixture. Here, a highly sensitive liquid filled core Photonic Crystal Fiber structure has been proposed to detect liquid mixture solution. Numerical investigation of the proposed structure is carried out by employing full vectorial Finite Element Method (FEM).展开更多
Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro ...Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro SSAO activity-based method was developed to screen inhibitors from 15 an- ti-obesity drugs. Among the fifteen anti-obesity drugs, four drugs including caffeine, fenfluramine, bumetanide and amfebutamone inhibited SSAO activity, and caffeine was the most effective one. When the concentration of caffeine was 1.4 mmol/L, the inhibition ratio was 31.9% and 18.8% in rabbit serum and rat adipose tissue, respectively. Inhibition of SSAO activity by caffeine was also confirmed in the in vivo study, showing the inhibition ratio of 15.6% on serum SSAO. Caffeine pro- vides a natural source of inhibition of SSAO activity and may be a promising inhibitor for the study of SSAO.展开更多
Small stone asphalt mixture（SSAM） was designed by Bailey method and coarse aggregate voids-filling method.The optimum asphalt content was determined by Marshal test.Surface texture depth for SSAM with different void...Small stone asphalt mixture（SSAM） was designed by Bailey method and coarse aggregate voids-filling method.The optimum asphalt content was determined by Marshal test.Surface texture depth for SSAM with different voidage,and the BPN of SSAM and SMA before after wet track abrasion were measured.The experimental results indicate that the surface texture depth increases with the decreasing of asphalt aggregate ratio.The SSAM with the optimal asphalt content has a good skid resistance.BPN of asphalt mixture decreases with the increasing of wearing time,but the extent of reduction is different.The reduction rate of BPN for SSAM is smaller than that of SMA,indicating that SSAM has a good skid resistance attenuation capacity.Finally,the other properties of SSAM are also evaluated,showing that the splitting strength and modulus and SSAM are higher than those of SMA,and the other properties of SSAM,such as high-temperature performance and water stability can also satisfy the technical requirements.展开更多
Prior to formation of the micelles of cationic surfactant (CSF), bromopyrogallol red (BPR) could exist in the forms of both monomer and oligomer After that, however, only BPR monomer existed. Nonionic surfactant OP-10...Prior to formation of the micelles of cationic surfactant (CSF), bromopyrogallol red (BPR) could exist in the forms of both monomer and oligomer After that, however, only BPR monomer existed. Nonionic surfactant OP-10 favoured the oligomerization of BPR monomer. The mixed micellar media prepared by mixing CSF and OP-10 in an appropriate ratio could be used for the sensitive and selective determination of Mo in Mo/W binary mixtures.展开更多
文摘目的探讨硫化铜(CuS)/氧化石墨烯(GO)/壳聚糖(CS)/纳米羟基磷灰石(nHA)复合材料(CGCHs)的抗菌和促成骨作用及其作用机制。方法采用水热法合成CuS/GO纳米颗粒,通过原位沉淀法合成CS/nHA支架和CGCHs支架,检测材料表征、光热转换性能和生物安全性,评估CGCHs组和近红外光(NIR)照射下CGCHs(CGCHs+NIR)组的细菌抑制效果及其对细菌生物膜相关基因表达的影响,观察CGCHs和CS/nHA不同材料组的促成骨分化和成骨、破骨相关基因表达。结果CGCHs是具有高度孔隙率的三维支架,在CuS/GO浓度为200μg/mL时CGCHs同时兼具良好的红外升温效果和生物安全性。琼脂糖平板涂菌和细菌死活染色结果均表明CGCHs+NIR组抗菌性能最佳,生物膜相关基因qPCR检测证实其具有抑制细菌生物膜相关基因表达的作用。茜素红染色结果表明CGCHs具有良好的体外促成骨性能,体外共培养3、7、14、21和28 d qPCR结果表明CGCHs对成骨早期和晚期相关基因表达均具有促进作用。与破骨细胞共培养结果可观察到CGCHs具有抑制破骨细胞形成的作用,细胞凋亡检测结果进一步验证这一结论,破骨分化相关基因qPCR检测结果表明,CGCHs主要通过抑制抗酒石酸酸性磷酸酶、组织蛋白酶K、CTR、P65和P38在共培养7、14 d的表达来抑制破骨细胞的分化。结论作为纳米复合材料,CGCHs生物安全性好,具有良好的红外光热协同抗菌作用,在促成骨分化的同时抑制破骨细胞分化,有望为感染性骨缺损治疗提供新的思路。
文摘Detection of liquid mixture of different volume ratio is very important in industrial purposes. The paper reports a sensing mechanism of binary liquid mixture for different volume fraction, based on the measurement of refractive index of the mixture. Here, a highly sensitive liquid filled core Photonic Crystal Fiber structure has been proposed to detect liquid mixture solution. Numerical investigation of the proposed structure is carried out by employing full vectorial Finite Element Method (FEM).
基金Supported by the Ministry of Science and Technology of China(2008AA12A218 2009BAK59B01)
文摘Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro SSAO activity-based method was developed to screen inhibitors from 15 an- ti-obesity drugs. Among the fifteen anti-obesity drugs, four drugs including caffeine, fenfluramine, bumetanide and amfebutamone inhibited SSAO activity, and caffeine was the most effective one. When the concentration of caffeine was 1.4 mmol/L, the inhibition ratio was 31.9% and 18.8% in rabbit serum and rat adipose tissue, respectively. Inhibition of SSAO activity by caffeine was also confirmed in the in vivo study, showing the inhibition ratio of 15.6% on serum SSAO. Caffeine pro- vides a natural source of inhibition of SSAO activity and may be a promising inhibitor for the study of SSAO.
基金Funded by the Program for Innovative Research Team in University (IRT1050)the Ministry of Transport Technology Project(201131982020)the Key Program of Shaanxi Natural Science Foundation for Basic Research Plan (2010JZ009)
文摘Small stone asphalt mixture（SSAM） was designed by Bailey method and coarse aggregate voids-filling method.The optimum asphalt content was determined by Marshal test.Surface texture depth for SSAM with different voidage,and the BPN of SSAM and SMA before after wet track abrasion were measured.The experimental results indicate that the surface texture depth increases with the decreasing of asphalt aggregate ratio.The SSAM with the optimal asphalt content has a good skid resistance.BPN of asphalt mixture decreases with the increasing of wearing time,but the extent of reduction is different.The reduction rate of BPN for SSAM is smaller than that of SMA,indicating that SSAM has a good skid resistance attenuation capacity.Finally,the other properties of SSAM are also evaluated,showing that the splitting strength and modulus and SSAM are higher than those of SMA,and the other properties of SSAM,such as high-temperature performance and water stability can also satisfy the technical requirements.
文摘Prior to formation of the micelles of cationic surfactant (CSF), bromopyrogallol red (BPR) could exist in the forms of both monomer and oligomer After that, however, only BPR monomer existed. Nonionic surfactant OP-10 favoured the oligomerization of BPR monomer. The mixed micellar media prepared by mixing CSF and OP-10 in an appropriate ratio could be used for the sensitive and selective determination of Mo in Mo/W binary mixtures.
