Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase

AIM: To construct fusion protein of a single-chain antibody (scFv) against transferrin receptor (TfR) with alkaline phosphatase(AP). METHODS: The VH-linker-VL,namely scFv gene,was prepared by amplifying the VH and VL genes from plasmid pGEM-T-VH and pGEM-T-VL with splicing overlap extension polymerase chain reaction (SOE PCR). After the ScFv gene was modified by 5/71 and Not I,it was subcloned into the secretory expression vector pUC19/119, and then was transformed into E.coli TG1.The positive colonies were screened by colony PCR and their expressions were induced by IPTG.ScFv gene was gained by digesting ScFv expression vector pUC19/119 with 5/71 and NotI restriction enzymes, then subcloned into expression vector pDAP2, followed by transformation in E.coli TG1.The positive colonies were selected by bacterial colony PCR.The expression of fusion protein (scFv-AP) was induced by IPTG.Its activity was detected by enzyme immunoassay. The molecular weights of scFv and scFv-AP were measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: The product of SOE PCR formed a band of 700 bp in agarose gel electrophoresis. SDS-PAGE demonstrated the molecular weight of scFv was 27 ku.Immunofluorescent assay (IFA) demonstrated its reactivity with TfR.The molecular weight of scFv-AP was 75 ku.Enzyme immunoassay showed that scFv-AP could specifically bind to human TfR and play AP activity. CONCLUSION: We have successfully prepared the anti-human TfR scFv and constructed the fusion protein of scFv and AP.It is promising for immunological experiments.

Relationship and significance between anti-b2-glycoproteinⅠantibodies and platelet activation state in patients with ulcerative colitis

AIM： To study the relationship between anti-β2- glycoprotein Ⅰ （aβ2GPⅠ） antibodies and platelet activation state in patients with ulcerative colitis （UC） and its significance. METHODS： Peripheral blood samples were collected from 56 UC patients （34 males and 22 females, aged 43.5 years, range 21-66 years）, including 36 at active stage and 20 at remission stage, and 25 sex-and age-matched controls. The level of aβ2GP Ⅰ was measured by ELISA. The platelet activation markers, platelet activation complex- Ⅰ （PAC- Ⅰ ） and P-selectin （CD62P） were detected by flow cytometry. RESULTS： The A value for IgG aβ2GP Ⅰ in the active UC group was 0.61 ± 0.13, significantly higher than that in the remittent UC and control groups （0.50 ± 0.13 and 0.22 ± 0.14, P 〈 0.01）. There was a significant difference between the two groups （P 〈 0.01）. The A value for IgM aβ2GP Ⅰ in the active and remittent UC groups was 0.43 ± 0.13 and 0.38 ± 0.12, significantly higher than that in the control group （0.20 ± 0.12, P 〈 0.01）. However, there was no significant difference between the two groups （P 〉 0.05）. The PAC- Ⅰ positive rate for the active and remittent UC groups was 30.6% ± 7.6% and 19.6% ± 7.8% respectively, significantly higher than that for the control group （6.3% ± 1.7%,P 〈 0.01）. There was a significant difference between the two groups （P 〈 0.01）. The CD62P positive rate for the active and remittent UC groups was 45.0% ± 8.8% and 31.9% ± 7.8% respectively, significantly higher than that for the control group （9.2% ± 2.7%, P 〈 0.01）. There was a significant difference between the two groups （P 〈 0.01）. In the active UC group, the more severe the state of illness was, the higher the A value for IgG aβ2GP Ⅰ was, and the positive rate for PAC-Ⅰ and CD62P was positively correlated with the state of illness （Faβ2GP Ⅰ = 3.679, P 〈 0.05; FPAC-Ⅰ （%） = 5.346, P 〈 0.01; and FCD62P （%） = 5. 418, P 〈 0.01）. Meanwhile, in the same state of illness, the A value for IgG aβ2GP Ⅰ was positively correlated to the positive rates for PAC-Ⅰ and CD62P. CONCLUSION： aβ2GP Ⅰ level, platelet activation state and their relationship of them are closely correlated with the pathogenesis and development of UC.

Selection for Anti-transferrin Receptor Bispecific T-cell Engager in Different Molecular Formats

Selecting an ideal molecular format from diverse structures is a major challenge in developing a bispecific antibody(BsAb).To choose an ideal format of anti-CD3 x anti-transferrin receptor(TfR)bispecific antibodies for clinical application,we constructed TfR bispecific T-cell engager(BiTE)in two extensively applied formats,including single-chain tandem singlechain variable fragments(scFvs)and double-chain diabodies,and evaluated their functional characterizations in vitro.Results demonstrated that TfR-BiTE in both formats directed potent killing of TfR+HepG2 cells.However,compared to two・chain diabodies,scFvs were more efficient in antigen binding and TfR target killing.Furthermore,different domain orders in scFvs would also be evaluated because single-TfR-CD3-His was preferable to single-CD3-TfR-His in immunotherapeutic strategies.Thus,the single-chain tandem TfR-CD3 format was favored for further investigation in cancer therapy.

Prevalence of Anti-Cardiolipin and Anti-β2 Glycoprotein Antibodies in Indian Systemic Lupus Erythematosus Patients

Anti-phospholipid antibodies (APA) like anti-cardiolipin antibodies (ACA) and anti-β2glycoprotien (anti-β2GP) are important cause of venous and arterial thrombosis and other occlusive vascular diseases. The prevalence of these antibodies in SLE patients at the time of diagnosis is not known in Indian SLE patients. This study was conducted to evaluate the prevalence of ACA and anti-β2GP autoantibodies in SLE patients and to correlate them with disease activity and immune parameters such as C3, C4 and CRP levels. where 85 SLE patients referred from Rheumatology Department, KEM hospital, Mumbai were studied. SLE disease activity was evaluated by SLE Disease Activity Index (SLEDAI) score at the time of evaluation. All patients studied were in an active stage of disease of which 37.6% patients had renal disorders, which were categorized as Lupus Nephritis (LN) and 62.3% patients did not show any renal manifestations (non-LN). ACA and anti-β2GP autoantibodies, to IgG and IgM subclasses were tested by ELISA. C3, C4 and CRP levels were detected by nephelometer. It was observed that 12.9% patients were IgG-ACA and IgM-ACA positive and ACA positivity was noted more among LN group Anti-β2GP autoantibody positivity was 27.1% for IgG and 31.8% for IgM., IgG-anti-β2GP antibodies were slightly higher in non-LN patients, whereas a higher incidence of IgM-anti-β2GP antibodies were detected in LN patients. Hence detection both ACA and anti-β2GP antibodies along with associated immune parameters were helpful to evaluate their possible association with disease severity in SLE patients. A long term follow up of patients having ACA and anti-β2GP antibodies without thrombotic event is also needed to detect their possible thrombotic event in future along with their clinical presentation.

Periodontitis and Inflammation: Plasma High Titer Naturally Occurring Anti-Glucan Antibodies Form Immune Complex with Streptococcus mutans Antigen

Atheromatous plaques usually contain antigens of the periodontitis-causing bacteria Streptococcus mutans though molecular mechanism of this incorporation remains unknown. Since vascular adhesion and inflammatory potential of Immune Complexes (IC) are known we investigated the naturally occurring plasma antibodies that recognize major antigens from S. mutans. S. mutans-binding plasma proteins (SMBP) prepared by affinity chromatography on a column of heat-killed S. mutans could recognize α- and β-linked glucose in dextran and yeast respectively but not galactose in glycoproteins. SMBP contained only three proteins, each corresponding in electrophoretic mobility to standard plasma IgG, IgA or IgM. The major positively and negatively charged protein antigens (PSMAg and NSMAg) isolated from S. mutans by electrophoresis and ion exchange chromatography respectively were recognized sugar-reversibly by the anti-β-glucan antibody (ABG) and though less avidly, by the dextran-binding immunoglobulin (DIg) in normal plasma. NSMAg addition resulted in near doubling of IC-bound immunoglobulins in immunoglobulin-rich fraction of plasma. IC isolated from above fraction after NSMAg addition had substantially more IgA and IgM content than total plasma immunoglobulins. IC formation by NSMAg was significantly inhibited by ABG- and DIg-specific sugars or by selective withdrawal of ABG or DIg from plasma. ABG and DIg being relatively high titer plasma antibodies IC formation with them suggested a possible route for vascular adhesion and damage by S. mutans and its antigens. Further, high IgA content of these ICs indicated their susceptibility to tissue uptake through cell surface galectin-1 for which IgA is the lone immunoglobulin ligand.

Prevalence, significance and predictive value of antiphospholipid antibodies in Crohn's disease

AIM: To assess the prevalence and stability of different antiphospholipid antibodies(APLAs) and their association with disease phenotype and progression in inflammatory bowel diseases(IBD) patients.METHODS: About 458 consecutive patients [Crohn's disease(CD): 271 and ulcerative colitis(UC): 187] were enrolled into a follow-up cohort study in a tertiary IBD referral center in Hungary. Detailed clinical phenotypes were determined at enrollment by reviewing the patients' medical charts. Disease activity, medical treatment and data about evolvement of complications or surgical interventions were determined prospectively during the follow-up. Disease course(development f complicated disease phenotype and need for surgery),occurrence of thrombotic events, actual state of diseaseactivity according to clinical, laboratory and endoscopic scores and accurate treatment regime were recorded during the follow-up,(median, 57.4 and 61.6 mo for CD and UC). Sera of IBD patients and 103 healthy controls(HC) were tested on individual anti-β2-Glycoprotein-I(anti-β2-GPI IgA/M/G), anti-cardiolipin(ACA IgA/M/G)and anti-phosphatidylserine/prothrombin(anti-PS/PT IgA/M/G) antibodies and also anti-Saccharomyces cerevisiae antibodies(ASCA IgA/G) by enzyme-linked immunosorbent assay(ELISA). In a subgroup of CD(n = 198) and UC patients(n = 103), obtaining consecutive samples over various arbitrary timepoints during the disease course, we evaluated the intraindividual stability of the APLA status. Additionally,we provide an overview of studies, performed so far, in which significance of APLAs in IBD were assessed.RESULTS: Patients with CD had significantly higher prevalence of both ACA(23.4%) and anti-PS/PT(20.4%) antibodies than UC(4.8%, p < 0.0001 and10.2%, p = 0.004) and HC(2.9%, p < 0.0001 and15.5%, p = NS). No difference was found for the prevalence of anti-β2-GPI between different groups(7.2%-9.7%). In CD, no association was found between APLA and ASCA status of the patients.Occurrence of anti-β2-GPI, ACA and anti-PS/PT was not different between the group of patients with active vs inactive disease state according to appropriate clinical, laboratory and endoscopic scores in CD as well as in UC patients. All subtypes of anti-β2-GPI and ACA IgM status were found to be very stable over time, in contrast ACA IgG and even more ACA IgA status showed significant intraindividual changes.Changes in antibody status were more remarkable in CD than UC(ACA IgA: 49.9% vs 23.3% and ACA IgG:21.2% vs 5.8%). Interestingly, 59.1% and 30.1% of CD patients who received anti-TNF therapy showed significant negative to positive changes in ACA IgA and IgG antibody status respectively. APLA status was not associated with the clinical phenotype at diagnosis or during follow-up, medical therapy, or thrombotic events and it was not associated with the probability of developing complicated disease phenotype or surgery in a Kaplan-Meier analysis.CONCLUSION: The present study demonstrated enhanced formation of APLAs in CD patients. However,presence of different APLAs were not associated with the clinical phenotype or disease course.

PGE₂Generation in Myocardium from Isolated Rat Atrium under Hypoxia and Reoxygenation Conditions. Effect of Anti-<i>β</i>₁IgG from Patients with Chronic Severe Periodontitis

Background: Hypoxia is one of the most frequently encountered stresses in health and disease. Methods: We compared the effects of an anti-β1 periodontal IgG (pIgG) and an authentic β1 adrenergic agonist, xamoterol, on isolated myocardium from rat atria contractility. We used an ELISA assay to measure the generation of PGE2 in vitro after the addition of either the antibody or the adrenergic agonist. We analyzed the myocardium histopathologically in the presence of both the antibody and/or the adrenergic agonist drug during normoxia, hypoxia and reperfusion conditions. Results: PGE2 generation increased during the hypoxia and was unchanged during reoxygenation period compared with the production of this prostanoid in atria during normoxia condition. A β1 specific adrenoceptor antagonist atenolol and the β1 synthetic peptide abrogated the increment of the prostanoid in the presence of pIgG but only atenolol due to it in the presence of xamoterol. The increment of PGE2 was dependent on the activation of cox-1 and cox-2 isoforms. Moreover, cox-2 was more active and produced more increments in the production of PGE2 in the presence of the pIgG than cox-1 activation. Histopathologically, studies of myocardium specimens during these different periods of the experimental protocol: basal (B), hypoxia (H) and reoxygenation (R), were also performed and showed tissue necrosis and edematization at the myocardium level. Conclusion: The phenomenon studied here supports the notion that PGE2 may be responsible for tissue edematization. PGE2 maybe acts as a beneficial modulator in the myocardium and prevents a major injury of it. The inflammation damage to the heart organ and cardiomyocytes caused by the actions of the antibodies in the course of heart lesions provoked by cardiovascular autoimmune disease, explains some of these results obtained in the present experiments. Further studies will be needed to establish the real role of PGE2 during hypoxia injury of the heart in the course of autoimmune diseases.

转铁蛋白受体单克隆抗体纳米载药系统治疗白血病的基础研究

目的:探讨转铁蛋白受体单克隆抗体(TfR mAb)纳米载药系统靶向治疗急性白血病的效果及其潜在的抗肿瘤机制。方法:合成纳米载药粒TfR mAb-聚乳酸-羟基乙酸(PLGA)-聚L-赖氨酸(PLL)-聚乙二醇(PEG)-柔红霉素(DNR)。急性髓性白血病细胞株HL60细胞经药物干预后,倒置荧光显微镜下观察细胞内DNR的累积;流式细胞技术(FCM)检测HL60细胞内DNR浓度及细胞凋亡率;Western blot测定凋亡相关蛋白Cleaved-caspase 3的表达量。结果:单药DNR组和TfR mAb-PLGA-PLL-PEG-DNR组HL60细胞内可见DNR自发荧光累积,且TfR mAb-PLGA-PLL-PEG-DNR组细胞内DNR浓度高于单药DNR组(P<0.05);FCM检测结果显示,TfR mAb-PLGA-PLL-PEG-DNR组细胞凋亡率高于单药DNR组(P<0.05);Western blot检测结果证实,TfR mAb-PLGA-PLL-PEG-DNR组凋亡相关蛋白Cleaved-caspase 3表达量明显高于单药DNR组(P<0.05)。结论:TfR mAb-PLGA-PLL-PEG纳米载药系统将化疗药物靶向作用于肿瘤细胞HL60,可通过凋亡途径增加药物的抗肿瘤能力。

高效表达人源可溶性转铁蛋白受体sTfR抗原及多克隆抗体制备和应用

通过多种策略构建人源可溶性转铁蛋白受体(sTfR)抗原的高效表达体系获得足量的sTfR抗原,并免疫得到稳定的兔多克隆抗体,以此制备高灵敏度的体外检测试剂盒。首先通过筛选表达体系,最终确定以人胚胎肾细胞293(HEK293)表达体系进行sTfR抗原的表达,表达量可达0.52 mg/mL。为进一步增强sTfR表达量,优选CMV启动子及白蛋白信号肽,使抗原的表达量进一步增强至0.86 mg/mL。随后优选0.3 mg的sTfR抗原为免疫剂量,持续5次免疫新西兰兔后可获得高效价的sTfR兔多克隆抗体,该抗体耦联胶乳微球制成试剂盒后进行样本检测,其检测效果准确稳定。sTfR试剂盒及原料的开发显著降低生产成本,也为其他检测项目的试剂盒开发提供参考意义。

Immune-mediated necrotizing myopathy:Report of two cases

BACKGROUND Immune-mediated necrotizing myopathy is a rare autoimmune myopathy characterized by muscle weakness and elevated serum creatine kinase,with unique skeletal muscle pathology and magnetic resonance imaging features.CASE SUMMARY In this paper,two patients are reported:One was positive for anti-signal recognition particle antibody,and the other was positive for anti-3-hydroxy-3-methylglutaryl coenzyme A reductase antibody.CONCLUSION The clinical characteristics and treatment of the two patients were analysed,and the literature was reviewed to improve the recognition,diagnosis,and treatment of this disease.

子宫内膜异位症患者血清和腹腔液中CA125和抗转铁蛋白抗体水平测定

目的:探讨CA125和抗转铁蛋白抗体(TFAb)在子宫内膜异位症(EMT)患者血清和腹腔液中的表达水平及其诊断价值。方法:采用ELISA法检测52例EMT(Ⅰ、Ⅱ期25例,Ⅲ、Ⅳ期27例)患者及40例对照血清和腹腔液中CA125和TFAb的水平。结果:3组血清和腹腔液中CA125的水平差异有统计学意义(F=21.997和45.551,P<0.001);EMTⅢ、Ⅳ期患者血清和腹腔液中CA125水平高于Ⅰ、Ⅱ期患者(P<0.05)。3组血清、腹腔液中CA125和TFAb阳性率的比较,差异有统计学意义(χ2=18.945、40.926和59.916、59.919,P<0.05)。采用CA125和TFAb串联试验对EMT诊断的特异性可达到100.0%,CA125和TFAb并联试验中腹腔液对EMT诊断的灵敏性为94.2%。结论:CA125和TFAb均有助于EMT的诊断,联合测定血清或腹腔液中CA125和TFAb可提高诊断的准确性。

抗人转铁蛋白受体单克隆抗体增强姜黄素对雄激素非依赖性前列腺癌的抑制效应

目的检测抗人转铁蛋白受体单克隆抗体(TfR mAb)对雄激素非依赖性前列腺癌细胞生物学行为的影响,了解抗人TfR mAb对姜黄素抗瘤作用的协同效应。方法利用杂交瘤细胞株制备抗人TfR mAb,分别采用CFSE染色、AnnexinⅤ-PI染色和PI染色法流式细胞仪检测抗人TfR mAb对PC-3细胞增殖、凋亡和细胞周期的影响。采用FITC标记的鼠抗人TfR mAb检测姜黄素对雄激素非依赖性前列腺癌细胞表面TfR表达的影响。采用流式细胞仪进一步检测抗人TfR mAb联合姜黄素对PC-3细胞增殖、凋亡和细胞周期的影响。结果抗人TfR mAb对PC-3细胞增殖、凋亡和细胞周期无明显影响(均P>0.05)。姜黄素能够显著上调雄激素非依赖性前列腺癌细胞表面TfR表达(P<0.05)。抗人TfR mAb可显著增强姜黄素对PC-3细胞凋亡的诱导作用(P<0.05),而不影响姜黄素对PC-3细胞增殖和细胞周期的影响(均P>0.05)。结论姜黄素可导致雄激素非依赖性前列腺癌细胞表面TfR表达增高。抗人TfR mAb单独作用对雄激素非依赖性前列腺癌细胞的生物学行为无显著影响,但抗人TfR mAb可以增强姜黄素对雄激素非依赖性前列腺癌细胞的凋亡效应。

子宫内膜异位症患者血清抗转铁蛋白抗体的表达及意义

目的建立一种ELISA方法 ,检测患者血清中抗转铁蛋白抗体 ,评价其对子宫内膜异位症诊断的价值。方法用转铁蛋白抗原包被 ,间接ELISA方法检测患者血清中抗转铁蛋白抗体。采集患者血清 ,分 3组 :盆腔内异症组 38例 ,Ⅰ、Ⅱ期 2 0例 ,Ⅲ期 9例 ,Ⅳ期 9例 ;妇科良性疾病对照组 30例 ;正常对照组 4 0例。结果内异症患者血清中抗转铁蛋白抗体阳性率高达 94 .7% ,正常对照组阳性率为 2 .5 % ,良性疾病对照组阳性率为 0 % ,内异症组与两对照组比较差异有显著意义 (P均 <0 .0 1 )。间接ELISA法诊断内异症临床敏感性为 94 .7% ,特异性为 98.6 % ,诊断符合率为 96 .5 %。内异症各组阳性率比较差异无显著意义 (P >0 .0 5 )。结论用转铁蛋白抗原包被的间接ELISA法检测血清中抗转铁蛋白抗体诊断内异症敏感性、特异性强 ,诊断符合率高 。

单一免疫法与联合免疫法检测大便潜血的临床应用评价

观察两种大便潜血实验方法—单一免疫法(胶体金单克隆抗体血红蛋白检测法)和联合免疫法(血红蛋白、转铁蛋白联合免疫法)检测的灵敏度、特异性和抗干扰性,以便在工作中避免假阳性和假阴性结果。用单一免疫法和联合免疫法检测人7种不同浓度血红蛋白液,对比其灵敏度。取人经常食用的6种动物血红蛋白检验单一免疫法和联合免疫法的特异性和准确度。比较单一免疫法和联合免疫法对107例经内窥镜检查确诊消化道出血患者的阳性检出率。结果显示,单一免疫法和联合免疫法的灵敏度和特异性相同,联合免疫法的稳定性和抗干扰性优于单一免疫法,单一免疫法可出现假阴性和假阳性反应。联合免疫法是目前检测大便潜血的较好的方法,将转铁蛋白作为补充检测手段是临床判断是否存在隐血的较有价值的方法。

抗转铁蛋白受体单链抗体原核表达载体的构建和表达

目的 构建抗转铁蛋白受体 (TfR)单链抗体原核表达载体 ,为进一步研究其效应奠定基础。方法 从抗TfR单克隆抗体重链和轻链可变区基因的克隆载体 pGEM T VH和 pGEM T VL中扩增重链可变区 (VH)和轻链可变区 (VL)基因 ,用重叠延伸PCR的方法 ,在VH和VL基因间引入连接短肽 (Linker) ,构建VH Linker VL的单链抗体 (singlechainFv ,scFv)基因。经NcoⅠ和NotⅠ酶切后亚克隆到原核分泌型表达载体pUC19/119上 ,转化和筛选后 ,阳性细菌经IPTG诱导表达。间接免疫荧光法 (IFA)及抗体封闭试验鉴定其抗体活性。结果 凝胶电泳可见重叠延伸PCR扩增产物约 70 0bp条带 ,SDS PAGE鉴定表达产物的分子量为 2 7kD左右 ,与scFv的理论值一致 ,IFA及抗体封闭试验证明表达产物有抗人TfR的活性。结论 本研究成功地构建并表达了抗人TfRscFv ,为抗人TfRscFv的应用奠定了基础。

贫血患者血清转铁蛋白受体测定的临床意义

采用 Dot- ELISA法定量测定了 6 8例不同贫血患者的血清转铁蛋白受体 (s Tf R)水平 ,发现缺铁性贫血、溶血性贫血和急性失血性贫血患者 s Tf R均显著高于正常人 (P<0 .0 0 1) ;而慢性再生障碍性贫血患者 s Tf R显著低于正常人 (P<0 .0 0 1)。缺铁性贫血 s Tf R水平与血清铁蛋白水平呈负相关 (P<0 .0 0 5) ,溶血性贫血 s Tf R与网织红细胞计数呈正相关 (P<0 .0 0 5) ,s Tf R可以反映机体贮存铁和骨髓红细胞系造血活动。认为 s Tf R测定为诊断缺铁性贫血和判断骨髓红细胞生成的一项具有实用价值的新指标。

子宫内膜异位症患者血清中靶抗体的检测和应用

目的 :建立一种ELISA方法 ,以检测子宫内膜异位症患者血清中抗转铁蛋白抗体。 方法 :用转铁蛋白包被的间接ELISA方法检测血清中的抗转铁蛋白抗体。血清标本来自 96例正常体检女性 ,6 4例妊娠妇女 ,72例儿科女童 ,以及 2 4例子宫内膜异位症患者。 结果 :子宫内膜异位症患者血清中的抗转铁蛋白抗体阳性率远远高于对照组 ,特异性为 99.0 % ,敏感度为 95 .8% ,准确性为 99.2 %。 结论 :子宫内膜异位症患者血清抗转铁蛋白抗体阳性率明显高于对照组 。

脑药物转运载体——抗转铁蛋白受体单链抗体的克隆表达及鉴定

为构建特异性的脑药物转运载体 ,分段合成了抗大鼠转铁蛋白受体的单链抗体基因 (Ox2 6 scfv) .经重叠PCR拼接成完整片段 ,克隆入pUC19载体中 ,测序正确后克隆到大肠杆菌表达载体pET 15b E .tag上 .IPTG诱导 ,表达产物分子量为 2 9kD ,约占菌体总蛋白量的 4 0 % .包涵体经 6mol L盐酸胍变性后 ,过SephacrylS 30 0HR分子筛柱复性蛋白 .免疫酶染色实验表明 ,该单链抗体能与转铁蛋白受体特异性结合 。

转铁蛋白受体单抗化疗药偶联物动脉灌注/栓塞治疗晚期恶性肿瘤

采用转铁蛋白受体单克隆抗体与表阿霉素或卡铂偶联物通过动脉灌注或栓塞治疗18例晚期恶性肿瘤。结果:9例肝癌中,完全缓角(CR)1例,部分缓解(PR)7例,无变化(NC)1例;3例肺癌中,PR2例,NC1例;其他6例中,1例壶腹癌及1例卵巢癌为CR,2例结肠癌术后复发及1例胰头癌术后复发为PR,1例肾癌术后腰椎转移为NC。无一病变加重,总有效率(CR+PR)达83.2％。病人治疗过程中,除一过性恶心、呕吐外,无特殊不良反应。