Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some case...Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some cases, the use of immunohistochemistry is important. Objectives: This paper aims to specify the p63 phenotypic profile of lesions diagnosed benign, with minimal suspect foci, difficult to interpret, HGPIN (high grade intraepithelial neoplasia) and LGPIN (low-grade prostatic intraepithelial neoplasia) and evaluate the manual technique of p63 immunohistochemistry. Patients and Method: This was a retrospective, descriptive study of prostate biopsies recorded in the PAC service of the HALD from January 1st, 2018 to December 31st, 2018. It was completed by a manual immunohistochemical study of the blocks enrolled from November 19th to December 4th, 2020 in the PAC department of the HPD. The studied parameters were: registry number, age, clinical stage, prostate volume, PSA level, microscopic appearance and p63 immunohistochemical profile. Results: Our study included 60 prostate biopsies. The ages of our patients varied from 45 to 77 years, with an average of 64.2 years and a standard deviation of 6.2. The majority of patients were at clinical stage cT2b (33%) with a prostate volume varying between 33.15 and 169.4 cc. The minimum value of PSA in our series is 5 ng/ml, the maximum being 100 ng/ml with an average level of 24.1 ng/ml and a standard deviation of 21.2. Our series included 50 adenomyomatous hyperplasias, 7 adenomyomatous hyperplasias associated with chronic prostatitis, 2 HGPIN and 1 LGPIN. After re-reading we found 5 discordant cases, which corresponded to minimal suspect foci (kappa = 0.5098). The p63 marking was informative in 53 cases, i.e. 88%, and non-informative in 7 cases, i.e. 12%. Among the uninformative markings, 2 were due to lack of tissue adhesion to the slides. Among the informative markings, 11 were negative. p63 immunohistochemistry was useful in all suspected foci and detected 6 other minimal foci of adenocarcinoma. Conclusion: The immunostaining with the anti-p63 antibody in the prostate cancer diagnosis is of considerable benefit. It made it possible to correct 11.3% of benign diagnosis in minimal malignant focus in our context. Despite the difficulties associated with the manual technique, it is possible to have an informative rate, similar to the automatic technique.展开更多
目的制备1株抗黑猩猩腺病毒68型(chimpanzee adenovirus type 68,AdC68)六邻体(hexon,hex)蛋白重组单克隆抗体(单抗)。方法表达并纯化AdC68-hex蛋白,免疫BALB/c小鼠,取其脾细胞与骨髓瘤细胞融合,经间接ELISA及细胞免疫染色法筛选得到1...目的制备1株抗黑猩猩腺病毒68型(chimpanzee adenovirus type 68,AdC68)六邻体(hexon,hex)蛋白重组单克隆抗体(单抗)。方法表达并纯化AdC68-hex蛋白,免疫BALB/c小鼠,取其脾细胞与骨髓瘤细胞融合,经间接ELISA及细胞免疫染色法筛选得到1株分泌抗AdC68-hex蛋白单抗的杂交瘤细胞。抽提杂交瘤细胞RNA进行测序,获得单抗重链与轻链可变区序列,分别与小鼠重链与轻链恒定区序列拼接得到小鼠全长IgG1κ抗体重链与轻链,并构建至真核细胞高效表达质粒。将重链与轻链质粒共同转染至哺乳动物细胞表达并纯化得到抗AdC68-hex重组单抗。将抗体用于滴定AdC68、重组AdC68及AdC68重组疫苗,并开展方法学验证。结果表达制备的AdC68-hex蛋白能有效诱导小鼠的病毒产生抗AdC68-hex抗体。抗AdC68-hex重组单抗用于重组AdC68滴定具备良好的专属性、耐用性,在4.56~9.57 lg感染单位/ml范围内具备良好的线性、准确度和精密度。结论制备的重组抗AdC68-hex单抗适用于AdC68及AdC68重组疫苗载体相关的基因治疗药物及疫苗等的检测。展开更多
BACKGROUND Xanthogranulomatous inflammation is characterized histologically by a collection of lipid-laden macrophages admixed with lymphocytes,plasma cells,neutrophils,and often multinucleated giant cells with or wit...BACKGROUND Xanthogranulomatous inflammation is characterized histologically by a collection of lipid-laden macrophages admixed with lymphocytes,plasma cells,neutrophils,and often multinucleated giant cells with or without cholesterol clefts.AIM To review the medical literature on xanthogranulomatous appendicitis(XGA).METHODS We present a patient with XGA and review published articles on XGA accessed via the PubMed,MEDLINE,Google Scholar,and Google databases.Keywords used were“appendix vermiformis,”“appendectomy,”“acute appendicitis,”and“XGA.”The search included articles published before May 2020,and the publication language was not restricted.The search included letters to the editor,case reports,review articles,original articles,and meeting presentations.Articles or abstracts containing adequate information about age,sex,clinical presentation,white blood cells,initial diagnosis,surgical approach,histopathological and immunohistochemical features of appendectomy specimens were included in the study.RESULTS A total of 29 articles involving 38 patients with XGA,were retrospectively analyzed.Twenty(52.6%)of the 38 patients,aged 3 to 78 years(median:34;IQR:31)were female,and the remaining 18(47.4%)were male.Twenty-five patients were diagnosed with acute appendicitis,ruptured appendicitis,or subacute appendicitis,and the remaining 13 patients underwent surgery for tumoral lesions of the ileocecal region.Twenty-two of the patients underwent urgent or semi-urgent surgery,and the remaining 16 patients underwent interval appendectomy.CONCLUSION Xanthogranulomatous inflammation rarely affects the appendix vermiformis.It is associated with significant diagnostic and therapeutic dilemmas due to its variable presentation.It is often associated with interval appendectomies,and a significant number of patients require bowel resection due to the common presentation of a tumoral lesion.XGA is usually identified retrospectively on surgical pathology and has no unique features in preoperative diagnostic studies.展开更多
文摘Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some cases, the use of immunohistochemistry is important. Objectives: This paper aims to specify the p63 phenotypic profile of lesions diagnosed benign, with minimal suspect foci, difficult to interpret, HGPIN (high grade intraepithelial neoplasia) and LGPIN (low-grade prostatic intraepithelial neoplasia) and evaluate the manual technique of p63 immunohistochemistry. Patients and Method: This was a retrospective, descriptive study of prostate biopsies recorded in the PAC service of the HALD from January 1st, 2018 to December 31st, 2018. It was completed by a manual immunohistochemical study of the blocks enrolled from November 19th to December 4th, 2020 in the PAC department of the HPD. The studied parameters were: registry number, age, clinical stage, prostate volume, PSA level, microscopic appearance and p63 immunohistochemical profile. Results: Our study included 60 prostate biopsies. The ages of our patients varied from 45 to 77 years, with an average of 64.2 years and a standard deviation of 6.2. The majority of patients were at clinical stage cT2b (33%) with a prostate volume varying between 33.15 and 169.4 cc. The minimum value of PSA in our series is 5 ng/ml, the maximum being 100 ng/ml with an average level of 24.1 ng/ml and a standard deviation of 21.2. Our series included 50 adenomyomatous hyperplasias, 7 adenomyomatous hyperplasias associated with chronic prostatitis, 2 HGPIN and 1 LGPIN. After re-reading we found 5 discordant cases, which corresponded to minimal suspect foci (kappa = 0.5098). The p63 marking was informative in 53 cases, i.e. 88%, and non-informative in 7 cases, i.e. 12%. Among the uninformative markings, 2 were due to lack of tissue adhesion to the slides. Among the informative markings, 11 were negative. p63 immunohistochemistry was useful in all suspected foci and detected 6 other minimal foci of adenocarcinoma. Conclusion: The immunostaining with the anti-p63 antibody in the prostate cancer diagnosis is of considerable benefit. It made it possible to correct 11.3% of benign diagnosis in minimal malignant focus in our context. Despite the difficulties associated with the manual technique, it is possible to have an informative rate, similar to the automatic technique.
文摘目的制备1株抗黑猩猩腺病毒68型(chimpanzee adenovirus type 68,AdC68)六邻体(hexon,hex)蛋白重组单克隆抗体(单抗)。方法表达并纯化AdC68-hex蛋白,免疫BALB/c小鼠,取其脾细胞与骨髓瘤细胞融合,经间接ELISA及细胞免疫染色法筛选得到1株分泌抗AdC68-hex蛋白单抗的杂交瘤细胞。抽提杂交瘤细胞RNA进行测序,获得单抗重链与轻链可变区序列,分别与小鼠重链与轻链恒定区序列拼接得到小鼠全长IgG1κ抗体重链与轻链,并构建至真核细胞高效表达质粒。将重链与轻链质粒共同转染至哺乳动物细胞表达并纯化得到抗AdC68-hex重组单抗。将抗体用于滴定AdC68、重组AdC68及AdC68重组疫苗,并开展方法学验证。结果表达制备的AdC68-hex蛋白能有效诱导小鼠的病毒产生抗AdC68-hex抗体。抗AdC68-hex重组单抗用于重组AdC68滴定具备良好的专属性、耐用性,在4.56~9.57 lg感染单位/ml范围内具备良好的线性、准确度和精密度。结论制备的重组抗AdC68-hex单抗适用于AdC68及AdC68重组疫苗载体相关的基因治疗药物及疫苗等的检测。
文摘BACKGROUND Xanthogranulomatous inflammation is characterized histologically by a collection of lipid-laden macrophages admixed with lymphocytes,plasma cells,neutrophils,and often multinucleated giant cells with or without cholesterol clefts.AIM To review the medical literature on xanthogranulomatous appendicitis(XGA).METHODS We present a patient with XGA and review published articles on XGA accessed via the PubMed,MEDLINE,Google Scholar,and Google databases.Keywords used were“appendix vermiformis,”“appendectomy,”“acute appendicitis,”and“XGA.”The search included articles published before May 2020,and the publication language was not restricted.The search included letters to the editor,case reports,review articles,original articles,and meeting presentations.Articles or abstracts containing adequate information about age,sex,clinical presentation,white blood cells,initial diagnosis,surgical approach,histopathological and immunohistochemical features of appendectomy specimens were included in the study.RESULTS A total of 29 articles involving 38 patients with XGA,were retrospectively analyzed.Twenty(52.6%)of the 38 patients,aged 3 to 78 years(median:34;IQR:31)were female,and the remaining 18(47.4%)were male.Twenty-five patients were diagnosed with acute appendicitis,ruptured appendicitis,or subacute appendicitis,and the remaining 13 patients underwent surgery for tumoral lesions of the ileocecal region.Twenty-two of the patients underwent urgent or semi-urgent surgery,and the remaining 16 patients underwent interval appendectomy.CONCLUSION Xanthogranulomatous inflammation rarely affects the appendix vermiformis.It is associated with significant diagnostic and therapeutic dilemmas due to its variable presentation.It is often associated with interval appendectomies,and a significant number of patients require bowel resection due to the common presentation of a tumoral lesion.XGA is usually identified retrospectively on surgical pathology and has no unique features in preoperative diagnostic studies.
