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Design and application of computer software for collecting color data about urine reagent strips
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作者 Longcong Chen Hongming Tan +5 位作者 Gaiqin Liu Changyou Chen Ping Chen Qifeng Jiang Bin Gao Xingliang Xiong 《Journal of Biomedical Science and Engineering》 2013年第8期782-787,共6页
Urine analysis is one of the most common and useful assay means in clinic for diagnose disease, especially urinary system disease and dry chemistry method is the most convenient, common and low cost about urine analys... Urine analysis is one of the most common and useful assay means in clinic for diagnose disease, especially urinary system disease and dry chemistry method is the most convenient, common and low cost about urine analysis. To expediently and efficiently analyze the corresponding relationships between different color data and semi-quantitative results about every urine item with dry chemistry way, in this paper, the design and application of effective software that may be used to collect color data about urine reagent strips are presented. First, we introduce the design procedure of this software. Second, its realization of design is given. Third, several application examples about collecting RGB original data by RS232 interface are shown. Then, RGB original data can be visually displayed by waveforms or curves and the data of cursor position may also be given. From the examples, we could confidently draw a conclusion that it is very convenient and effective to study on the laws of color variation about reagent pieces on reagent strip and it has met our original designed demands. 展开更多
关键词 SEMI-QUANTITATIVE Results dry CHEMISTRY Method reagent Pieces URINE reagent STRIP
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不同试剂处理对菲牛蛭冻干粉抗凝活性的影响研究 被引量:2
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作者 魏恒 李敏 +2 位作者 段红宇 黄潇 张德林 《成都中医药大学学报》 2015年第1期37-40,共4页
目的:研究比较不同试剂处理对菲牛蛭冻干粉抗凝血活性的影响。方法:采用凝血酶直接滴定法对不同试剂处理后的菲牛蛭冻干粉的抗凝血活性进行测定。结果:与对照组相比较,乙酸乙酯、正己烷、环己烷、丙酮、石油醚、蒸馏水6种试剂对菲牛蛭... 目的:研究比较不同试剂处理对菲牛蛭冻干粉抗凝血活性的影响。方法:采用凝血酶直接滴定法对不同试剂处理后的菲牛蛭冻干粉的抗凝血活性进行测定。结果:与对照组相比较,乙酸乙酯、正己烷、环己烷、丙酮、石油醚、蒸馏水6种试剂对菲牛蛭冻干粉抗凝血活性基本无影响;甲醇和乙醇对其活性损失最大,超过33%;氯仿、二氯甲烷损失较小,接近17%。结论:菲牛蛭冻干粉抗凝血活性强弱与其处理的试剂有较大关系。在菲牛蛭深加工时,建议选择石油醚、乙酸乙酯、蒸馏水3种试剂,既可保证其安全性,也能有效保留菲牛蛭冻干粉的抗凝血活性,最大程度利用资源。 展开更多
关键词 菲牛蛭 冻干粉 抗凝血活性 不同试剂
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基于核酸预混室温储运技术的荧光定量PCR快速检测鼠疫耶尔森氏菌
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作者 谢辉 周蕾 +1 位作者 祁芝珍 邵高祥 《中国人兽共患病学报》 CAS CSCD 北大核心 2021年第9期783-787,共5页
目的实现荧光定量PCR技术在鼠疫现场和基层的应用。方法本试验将已建立的基于核酸预混室温储运技术的荧光定量PCR方法应用于我国各鼠疫疫源地野生菌株的检测,进行特异性评价。选取分布于我国境内不同鼠疫疫源地野生鼠疫耶尔森氏菌株、... 目的实现荧光定量PCR技术在鼠疫现场和基层的应用。方法本试验将已建立的基于核酸预混室温储运技术的荧光定量PCR方法应用于我国各鼠疫疫源地野生菌株的检测,进行特异性评价。选取分布于我国境内不同鼠疫疫源地野生鼠疫耶尔森氏菌株、鼠疫减毒菌株、耶尔森菌属近缘菌株假结核菌及小肠结肠炎菌进行荧光定量PCR检测。结果55株鼠疫耶尔森氏菌及鼠疫减毒菌株扩增阳性,假结核耶尔森氏菌、小肠结肠炎耶尔森氏菌无阳性扩增,冻干试剂在室温25℃和37℃条件下可保存6个月,敏感性与冷冻保存无差异,核酸扩增诊断可在1 h内完成。结论应用基于核酸预混室温储运技术的荧光定量PCR方法对我国各鼠疫疫源地55株鼠疫耶尔森氏菌检测呈现高度特异性,本试验冻干试剂具有可室温保存、便于运输、检测结果精准、快速等特点,具有较好的鼠疫现场应用前景。 展开更多
关键词 室温储运 荧光定量PCR 鼠疫耶尔森氏菌 冻干试剂
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LOW-VALENT TITANIUM INDUCED REDUCTIVE CROSS-COUPLING REACTION OF ACYL CHLORIDES WITH DIARYL KETONES
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作者 Da Qing SHI Department of Chemistry,Xuzhou Teachers Collegs Xuzhou 221009Wei Xing CHEN Department of Chemistry,Nanjing University,Nanjing 210008 《Chinese Chemical Letters》 SCIE CAS CSCD 1993年第8期681-682,共2页
Treatment of acyl chlorides and diaryl ketones with anactivated Ti(o)reagent,prepared by reduction of TiCl<sub>4</sub> with Znpowder,effects an intermolecular reductive cross-coupling reactionleading to ... Treatment of acyl chlorides and diaryl ketones with anactivated Ti(o)reagent,prepared by reduction of TiCl<sub>4</sub> with Znpowder,effects an intermolecular reductive cross-coupling reactionleading to ketones. 展开更多
关键词 INTERMOLECULAR reagent REDUCTIVE valent titanium tempera SLURRY Schultz ARGON DRIED
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