Study Nanostructure of a Biomaterial with Medicine Therapeutic Activity

In Mexico, the medicinal plants have been for used since ancient times and still continue to use by rural populations to treat conditions such as infectious diseases (skin, gastrointestinal or respiratory), metabolics, inflammatory problems, among others. This work includes a proposal that initiates the study and development of new biomaterials or vehicles with therapeutic application. The nanomolecular interaction of the organic extract of a plant (EOV) Distictis buccinatoria (dc) with different materials inert supports of inorganic type (bentonite, gel) determining their and combined anti-inflammatory activity is studied individually [1] [2]. The importance of the characterization of biomaterials by scanning electron microscopy (SEM) is ability to analyze the structure on the surface of materials (organic, inorganic and biological) in the micro and nanometrics levels in order to correlate it with the sample surface properties such as roughness, interfacial phenomena, etc. With this technique we can also perform the chemical analysis at different levels (micro and nano) for information on the nature and composition of the materials [3] [4]. The biomaterial shows that higher anti-inflammatory activity is to be generated through the combination of EOV-gel, the nanostructure interaction SEM shows integrated particles agglome-rated form and porous due to the presence of EOV and some flat areas indicated the gel preponderance situation generated by the low interaction between materials of organic type and polar type (gel).

Co-delivery of Andrographolide and Notch1-targeted siRNA to Macrophages with Polymer-based Nanocarrier for Enhanced Anti-inflammation

Chronic inflammatory responses induced by macrophages play a pivotal role in the progression of atherosclerosis. In the present study, a multifunctional nanocarrier based on poly（ethylene glycol）-block-poly（L-aspartic acid） grafted with diethylenetriamine, lysine and cholic acid （PEG-PAsp（DETA）-Lys-CA2） polymer was synthesized for co-delivery of andrographolide and siRNA targeting Notchl gene to alleviate the inflammatory response in macrophages. The nanocarrier exerted low cytotoxicity as well as high performance in drug/siRNA co-delivery. In vitro studies demonstrated the co-delivery of andrographolide and Notchl siRNA not only significantly inhibited lipopolysaccharide （LPS）-activated interleukin-6 （IL-6） and monocytes chemotactic protein 1 （MCP-1） expression as well as blocked nuclear factor-rd3 （NF-rd3） signal activation, but also interfered the Notchl gene expression and increased anti-inflammatory cytokines such as interleukin-10 （IL-10） and arginase-1 expression obviously in macrophages. These results suggested that the combination therapy based on Notchl siRNA and andrographolide co-delivered nanocarrier, i.e. suppressing the expression of proinflammatory cytokines while simultaneously increasing anti-inflammatory factors expression, be a feasible strategy for atherosclerosis treatment.