OBJECTIVE: To explore the clinical effect of Huangqi and Zeling granule combined with Zhushui No.l paste plus conventional therapy on patients with cirrhosi.METHODS: Totally 90 patients with liver cirrhosis were rando...OBJECTIVE: To explore the clinical effect of Huangqi and Zeling granule combined with Zhushui No.l paste plus conventional therapy on patients with cirrhosi.METHODS: Totally 90 patients with liver cirrhosis were randomly assigned into two groups: control group and study group. They all met the inclusion criteria. The patients in the control group were treated by conventional treatment; the patients in the study group received Huangqi and Zeling granule and Zhushui No. 1 paste along with the conventional therapy.The paste was applied on the acupoint of shenque. The levels of LPS, ET-1, NO and AM ON were all measured before and after treatment. The depth of ascites, the change of diameter portal and size of spleen were performed by abdominal ultrasonography Adverse events were observed and documented.RESULTS: The levels of AMON, LPS, ET-1 and NO were all reduced after treatment which were more obviously in study group(P<0.05). The depth decreased in both study group and control group after treatment. However, the decrease was more in the study group than in the control group(P<0.05).CONCLUSION: The effect of Huangqi and Zeling granule plus Zhushui No. 1 paste combined with routine conventional therapy on cirrhosis was better than that of using the routine conventional therapy alone.展开更多
Objective:To elucidate the potential anti-inflammatory mechanisms of Rhamnus crenata leaf extracts using RAW264.7 cells.Methods:We used 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay to measure ce...Objective:To elucidate the potential anti-inflammatory mechanisms of Rhamnus crenata leaf extracts using RAW264.7 cells.Methods:We used 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay to measure cell viability.Nitric oxide(NO)production was measured using Griess reagent.Western blotting and RT-PCR assays were carried out for analyzing the protein and gene expressions of pro-inflammatory mediators,respectively.Moreover,PD98059(ERK1/2 inhibitor),SB203580(p38 inhibitor),SP600125(JNK inhibitor),and BAY11-7082(NF-κB inhibitor)were used to evaluate the anti-inflammatory mechanism of Rhamnus crenata leaf extract.Results:Rhamnus crenata leaf extracts significantly inhibited the production of the pro-inflammatory mediators such as NO,iNOS,COX-2,IL-1β,and TNF-αin lipopolysaccharide(LPS)-stimulated RAW264.7 cells.Rhamnus crenata leaf extracts also suppressed LPS-induced degradation of IκB-αand nuclear accumulation of p65,which resulted in the inhibition of NF-κB activation in RAW264.7 cells.Additionally,the extracts attenuated the phosphorylation of p38,ERK1/2,and JNK in LPS-stimulated RAW264.7 cells.Moreover,HO-1 expression induced by Rhamnus crenata leaf extracts was significantly downregulated by SB230580,PD98059,SP600125 and BAY11-7082.Conclusions:Rhamnus crenata leaf extract may upregulate HO-1 expression through inhibition of p38,ERK1/2,and NF-κB activation,which may contribute to the anti-inflammatory activity of the extracts.Rhamnus crenata leaf extracts may have great potential for the development of anti-inflammatory drugs to treat acute and chronic inflammatory diseases.展开更多
The effects of Lobenzarit disodium (CCA) upon the activity of interleukin-1(IL-1) of peritoneal macrophages(PMΦ)in normal and in adjuvant arthritis AA) rats and on the release of hydrogen peroxide(H_2O_2)of rat perit...The effects of Lobenzarit disodium (CCA) upon the activity of interleukin-1(IL-1) of peritoneal macrophages(PMΦ)in normal and in adjuvant arthritis AA) rats and on the release of hydrogen peroxide(H_2O_2)of rat peritoneal macrophages were studied. CCA 10～200 μg/ml could inhibit IL-1activity in normal rat in vitro;CCA 10 and 50 mg/kg could depress the level of increased IL-1 in AA rats in vivo;CCA 5～100μg/ml could inhibit the release of H_2O_2 from rat PMΦin vitro.The results suggest that these effects could be one of the mechanisms of anti-inflammatory action of CCA.展开更多
Objectives: The existing inflammatory models are concentrated in relatively complex medical fields, and most of them use a single type of cell, and the induction conditions are not uniform, so the current LPS-induced ...Objectives: The existing inflammatory models are concentrated in relatively complex medical fields, and most of them use a single type of cell, and the induction conditions are not uniform, so the current LPS-induced inflammation model is less conducive to the study of skin inflammation. The aim of this research is to enhance the existing LPS-induced inflammation model and establish a skin inflammation model that is suitable for the swift screening of anti-inflammatory agents in the cosmetics industry. Methods: LPS was used to induce inflammatory responses in KC and THP-1 cells. Enzyme-linked immunosorbent assay (ELISA) was employed to assess the levels of IL-1α, IL-8, and TNF-α in the two cell types, while the DCFH-DA probe was utilized to label the levels of reactive oxygen species (ROS) in both cell types. Results: In KC cells, 10 μg/mL of LPS induced a significant upregulation of IL-8 but did not result in elevated expression of IL-1α. However, at 100 μg/mL of LPS, both IL-8 and IL-1α were highly expressed in KC cells. LPS concentrations ranging from 0.01 to 100 μg/mL failed to stimulate TNF-α production in KC cells but induced a gradient increase in ROS levels. In THP-1 cells, LPS concentrations from 0.01 to 100 μg/mL did not induce IL-1α production but significantly elevated IL-8 and led to a gradient increase in TNF-α and ROS. After treatment with 100 μg/mL of LPS, the cosmetic ingredient Rucika KGM mitigated the elevated levels of IL-1α, IL-8, and ROS in LPS-induced KC cells and IL-8 and ROS in THP-1 cells. Conclusion: This study has successfully developed an application-oriented model suitable for investigating skin inflammation, enabling the rapid and comprehensive screening of cosmetic ingredients with anti-inflammatory activity. .展开更多
基金Project Supported by Science and Technology Program of Henan Province(Grant No.:132102310154)
文摘OBJECTIVE: To explore the clinical effect of Huangqi and Zeling granule combined with Zhushui No.l paste plus conventional therapy on patients with cirrhosi.METHODS: Totally 90 patients with liver cirrhosis were randomly assigned into two groups: control group and study group. They all met the inclusion criteria. The patients in the control group were treated by conventional treatment; the patients in the study group received Huangqi and Zeling granule and Zhushui No. 1 paste along with the conventional therapy.The paste was applied on the acupoint of shenque. The levels of LPS, ET-1, NO and AM ON were all measured before and after treatment. The depth of ascites, the change of diameter portal and size of spleen were performed by abdominal ultrasonography Adverse events were observed and documented.RESULTS: The levels of AMON, LPS, ET-1 and NO were all reduced after treatment which were more obviously in study group(P<0.05). The depth decreased in both study group and control group after treatment. However, the decrease was more in the study group than in the control group(P<0.05).CONCLUSION: The effect of Huangqi and Zeling granule plus Zhushui No. 1 paste combined with routine conventional therapy on cirrhosis was better than that of using the routine conventional therapy alone.
基金supported by the research project of the National Institute of Forest Science(project No.FP0400-2019-01-2022).
文摘Objective:To elucidate the potential anti-inflammatory mechanisms of Rhamnus crenata leaf extracts using RAW264.7 cells.Methods:We used 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay to measure cell viability.Nitric oxide(NO)production was measured using Griess reagent.Western blotting and RT-PCR assays were carried out for analyzing the protein and gene expressions of pro-inflammatory mediators,respectively.Moreover,PD98059(ERK1/2 inhibitor),SB203580(p38 inhibitor),SP600125(JNK inhibitor),and BAY11-7082(NF-κB inhibitor)were used to evaluate the anti-inflammatory mechanism of Rhamnus crenata leaf extract.Results:Rhamnus crenata leaf extracts significantly inhibited the production of the pro-inflammatory mediators such as NO,iNOS,COX-2,IL-1β,and TNF-αin lipopolysaccharide(LPS)-stimulated RAW264.7 cells.Rhamnus crenata leaf extracts also suppressed LPS-induced degradation of IκB-αand nuclear accumulation of p65,which resulted in the inhibition of NF-κB activation in RAW264.7 cells.Additionally,the extracts attenuated the phosphorylation of p38,ERK1/2,and JNK in LPS-stimulated RAW264.7 cells.Moreover,HO-1 expression induced by Rhamnus crenata leaf extracts was significantly downregulated by SB230580,PD98059,SP600125 and BAY11-7082.Conclusions:Rhamnus crenata leaf extract may upregulate HO-1 expression through inhibition of p38,ERK1/2,and NF-κB activation,which may contribute to the anti-inflammatory activity of the extracts.Rhamnus crenata leaf extracts may have great potential for the development of anti-inflammatory drugs to treat acute and chronic inflammatory diseases.
文摘The effects of Lobenzarit disodium (CCA) upon the activity of interleukin-1(IL-1) of peritoneal macrophages(PMΦ)in normal and in adjuvant arthritis AA) rats and on the release of hydrogen peroxide(H_2O_2)of rat peritoneal macrophages were studied. CCA 10～200 μg/ml could inhibit IL-1activity in normal rat in vitro;CCA 10 and 50 mg/kg could depress the level of increased IL-1 in AA rats in vivo;CCA 5～100μg/ml could inhibit the release of H_2O_2 from rat PMΦin vitro.The results suggest that these effects could be one of the mechanisms of anti-inflammatory action of CCA.
文摘Objectives: The existing inflammatory models are concentrated in relatively complex medical fields, and most of them use a single type of cell, and the induction conditions are not uniform, so the current LPS-induced inflammation model is less conducive to the study of skin inflammation. The aim of this research is to enhance the existing LPS-induced inflammation model and establish a skin inflammation model that is suitable for the swift screening of anti-inflammatory agents in the cosmetics industry. Methods: LPS was used to induce inflammatory responses in KC and THP-1 cells. Enzyme-linked immunosorbent assay (ELISA) was employed to assess the levels of IL-1α, IL-8, and TNF-α in the two cell types, while the DCFH-DA probe was utilized to label the levels of reactive oxygen species (ROS) in both cell types. Results: In KC cells, 10 μg/mL of LPS induced a significant upregulation of IL-8 but did not result in elevated expression of IL-1α. However, at 100 μg/mL of LPS, both IL-8 and IL-1α were highly expressed in KC cells. LPS concentrations ranging from 0.01 to 100 μg/mL failed to stimulate TNF-α production in KC cells but induced a gradient increase in ROS levels. In THP-1 cells, LPS concentrations from 0.01 to 100 μg/mL did not induce IL-1α production but significantly elevated IL-8 and led to a gradient increase in TNF-α and ROS. After treatment with 100 μg/mL of LPS, the cosmetic ingredient Rucika KGM mitigated the elevated levels of IL-1α, IL-8, and ROS in LPS-induced KC cells and IL-8 and ROS in THP-1 cells. Conclusion: This study has successfully developed an application-oriented model suitable for investigating skin inflammation, enabling the rapid and comprehensive screening of cosmetic ingredients with anti-inflammatory activity. .
