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Identification of the epitopes on HCV core protein recognized by HLA-A2 restricted cytotoxic T lymphocytes 被引量:11
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作者 Hong-Chao Zhou De-Zhong Xu Xue-Ping Wang Jing-Xia Zhang Ying-Huang Yong-Ping Yan Yong Zhu Bo-Quan Jin Department of Epidemiology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,ChinaDepartment of Immunology,the Fourth Military Medical University,Xi’an 710033,Shaanxi Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第4期583-586,共4页
AIM: To identify hepatitis C virus(HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL). METHODS: Utilizing the method of computer prediction followed by a 4h(51)Cr release assay con... AIM: To identify hepatitis C virus(HCV) core protein epitopes recognized by HLA-A2 restricted cytotoxic T lymphocyte (CTL). METHODS: Utilizing the method of computer prediction followed by a 4h(51)Cr release assay confirmation. RESULTS: The results showed that peripheral blood mononuclear cells (PBMC) obtained from two HLA-A2 positive donors who were infected with HCV could lyse autologous target cells labeled with peptide &quot;ALAHGVRAL (core 150-158)&quot;. The rates of specific lysis of the cells from the two donors were 37.5% and 15.8%, respectively. Blocking of the CTL response with anti-CD4 mAb caused no significant decrease of the specific lysis. But blocking of CTL response with anti-CD8 mAb could abolish the lysis. CONCLUSION: The peptide (core 150-158) is the candidate epitope recognized by HLAA2 restricted CTL. 展开更多
关键词 Amino Acid Sequence antibodies viral B-LYMPHOCYTES Cell Line Epitope Mapping HLA-A2 Antigen HEPACIVIRUS Hepatitis C Humans Peptide Fragments Predictive Value of Tests Research Support Non-U.S. Gov't T-Lymphocytes Cytotoxic viral Core Proteins
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Humoral and cellular immunogenecity of DNA vaccine based on hepatitis B core gene in rhesus monkeys 被引量:19
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作者 Zu Hu Huang1 Hui Zhuang2 +4 位作者 Shan Lu3 Ren Hua Guo1 Guo Min Xu2 Jie Cai1 Wan Fu Zhu2 1Department of Infectious Diseases. The First Affiliated Hospital of Nanjing Medical University, Nenjing 210029, Jiangsu Province. China2Faculty of Microbiology, Beijing University, Beijing 100000, China3University of Massachusetts Medical Center 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期102-106,共5页
INTRODUCTIONHepatitis B virus (HBV) is the most commonetiologic agent for infectious liver diseases. It isestimated that there are more than 250 millionchronic HBV carriersin the world today and thereis a significant ... INTRODUCTIONHepatitis B virus (HBV) is the most commonetiologic agent for infectious liver diseases. It isestimated that there are more than 250 millionchronic HBV carriersin the world today and thereis a significant association among persistentinfection, liver cirrhosis and hepatocellularcarcinoma[1-3]. 展开更多
关键词 Vaccines DNA Animals antibodies viral Antibody Formation Antibody Specificity Cell Division Cells Cultured Enzyme-Linked Immunosorbent Assay Female Hepatitis B control Hepatitis B Core Antigens Immunity Cellular Immunoglobulin G Interferon Type II INTERLEUKIN-4 Leukocytes Mononuclear Macaca mulatta Male Research Support Non-U.S. Gov't
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A comparative study on serologic profiles of virus hepatitis B 被引量:6
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作者 BoYOUl Cho Moran Ki Hung Bae Park 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期107-110,共4页
INTRODUCTIONHepatitis B viral infection, one of the most-prevalent liver disorders in China and Korea, is aserious infectious disease as it has the potential ofprogressing into liver cirrhosis and primary hepaticcarci... INTRODUCTIONHepatitis B viral infection, one of the most-prevalent liver disorders in China and Korea, is aserious infectious disease as it has the potential ofprogressing into liver cirrhosis and primary hepaticcarcinoma. China and Korea both belong to high-risk endemic regions of viral hepatitis[1]. TheHBsAg positive rates in China ranged from 6.9% -17.9% by age, race and test methods[2-5]. 展开更多
关键词 Adult Age Distribution antibodies viral China Comparative Study Enzyme-Linked Immunosorbent Assay Ethnic Groups Female Hepatitis B Hepatitis B Surface Antigens Hepatitis B virus Humans Korea Male Middle Aged Research Support Non-U.S. Gov't Seroepidemiologic Studies Sex Distribution
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Establishment of transgenic mouse harboring hepatitis B virus (adr subtype) genomes 被引量:9
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作者 Yi Ping Hu1 Wei Jiang Hu1 +7 位作者 Wen Chao Zheng2 Jian Xiu Li1 De Shun Dai1 Xin Min Wang1 Shu Zhong Zhang1 Hong Yu Yu3 Wei Sun4 Guang Rong Hao4 1Department of Cell Biology, Second Military Medical University, Shanghai 200433, China2University of Wisconsin, Madison, WI 53705, USA3Department of Pathology, Second Military Medical University, Shanghai 200433, China4Center of laboratory Animals, Second Military Medical University, Shanghai 200433, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期111-114,共4页
INTRODUCTIONHepatitis B virus (HBV) belongs to the group ofhepatovirus, a major pathogen of human acute andchronic hepatitis B[1 4], which has a very closeassociation with human hepatocellular carcinoma(HCC)[5-8], For... INTRODUCTIONHepatitis B virus (HBV) belongs to the group ofhepatovirus, a major pathogen of human acute andchronic hepatitis B[1 4], which has a very closeassociation with human hepatocellular carcinoma(HCC)[5-8], For example, a statistical data from ahospital in Shanghai showed that 80% of HCCpatients were positive for HBsAg ( personalcommunication). 展开更多
关键词 Genome viral Animals antibodies viral DNA viral Disease Models Animal Gene Expression Regulation viral Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis B virus Kidney Liver MICE Mice Transgenic MICROINJECTIONS Microscopy Electron Polymerase Chain Reaction Research Support Non-U.S. Gov't Virus Integration
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Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells 被引量:3
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作者 Hao Ren Fen Lu Zhu +2 位作者 Shi Ying Zhu Yan Bin Song Zhong Tian Qi Department of Microbiology, Second Military Medical University, Shanghai 200433, China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第1期98-101,共4页
INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agen... INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agent independently and almost simultaneouslyand named hepatitis G virus and GB virus C,respectively[4-7]. 展开更多
关键词 Animals antibodies viral Blotting Western Cell Line Electrophoresis Polyacrylamide Gel Flaviviridae Infections GB virus C purification Gene Expression Regulation viral Plasmids Polymerase Chain Reaction Recombinant Proteins Research Support Non-U.S. Gov't SPODOPTERA Transfection viral Nonstructural Proteins
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Diagnostic value of human cytomegalovirus late mRNA detection in active intrauterine infection
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作者 姜宏 闻良珍 凌霞珍 《Chinese Medical Journal》 SCIE CAS CSCD 2002年第10期1507-1509,共3页
OBJECTIVE: To study the diagnostic value of human cytomegalovirus (HCMV) late mRNA detection in active intrauterine infection. METHODS: The HCMV late mRNA in peripheral blood of 42 HCMV IgM positive pregnant women and... OBJECTIVE: To study the diagnostic value of human cytomegalovirus (HCMV) late mRNA detection in active intrauterine infection. METHODS: The HCMV late mRNA in peripheral blood of 42 HCMV IgM positive pregnant women and their fetal attachments (such as chorionic villi, amniotic fluid, umbilical blood and placenta) were detected by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Late mRNA was detected in 23 of 42 HCMV IgM positive cases, a rate of 54.3%. Fetal appendages in 13 cases of late mRNA positive mothers were also tested, of which 7 were positive, with a vertical transmission rate of 53.8%. In 12 late mRNA negative mothers, only 1 case of fetal appendages tested was positive, with a vertical transmission rate of 8.3%. There was significant difference between the transmission rates of these two groups. CONCLUSIONS: Positive results of HCMV IgM cannot accurately reflect the activity of HCMV at the time of testing. However, the activity of HCMV is closely related to the mother-fetus vertical transmission rate. As an indicator of active HCMV infection, late mRNA can not only reflect the mother-fetus transmission rate during active HCMV infection, but also provide some information about HCMV activity in fetal tissue. 展开更多
关键词 antibodies viral CYTOMEGALOVIRUS Cytomegalovirus Infections Female Humans Immunoglobulin M Pregnancy Pregnancy Complications Infectious RNA Messenger Research Support Non-U.S. Gov't Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity
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Humoral immune responses in rabbits induced by an experimental inactivated severe acute respiratory syndrome coronavirus vaccine prepared from F69 strain 被引量:1
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作者 张传海 郭中敏 +11 位作者 郑焕英 陆家海 王一飞 鄢心革 赵勇 杜雄威 张欣 方苓 凌文华 戚树源 余新炳 钟南山 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第11期1625-1629,共5页
BACKGROUND: The etiologic agent of severe acute respiratory syndrome (SARS) has been confirmed to be a novel coronavirus (CoV), namely SARS-CoV. Developing safe and effective SARS-CoV vaccines is essential for us to p... BACKGROUND: The etiologic agent of severe acute respiratory syndrome (SARS) has been confirmed to be a novel coronavirus (CoV), namely SARS-CoV. Developing safe and effective SARS-CoV vaccines is essential for us to prevent the possible reemergence of its epidemic. Previous experiences indicate that inactivated vaccine is conventional and more hopeful to be successfully developed. Immunogenicity evaluation of an experimental inactivated SARS-CoV vaccine in rabbits was conducted and reported in this paper. METHODS: The large-scale cultured SARS-CoV F69 strain was inactivated with 0.4% formaldehyde and purified, then used as the immunogen combined with Freund's adjuvant. Eight adult New Zealand rabbits were immunized four times with this experimental inactivated vaccine. Twelve sets of rabbit serum were sampled from the third day to the seventy-fourth day after the first vaccination. The titers of specific anti-SARS-CoV IgG antibody were determined by indirect enzyme-linked immunosorbent assay, and the neutralizing antibody titers were detected with micro-cytopathic effect neutralization test. RESULTS: Rapid and potent humoral immune responses were induced by the inactivated SARS-CoV vaccine in all the eight test rabbits. Titers of both specific IgG antibody and neutralizing antibody peaked at about six weeks after first vaccination, with the maximum value of 1:81 920 and 1:20 480, respectively. After that, serum antibody levels remained at a plateau or had a slight decrease, though two boosters were given in the succedent 4 to 5 weeks. Cross neutralization response existed between SARS-CoV F69 strain and Z2-Y3 strain. CONCLUSIONS: The inactivated SARS-CoV vaccine made from F69 strain owns strong immunogenicity, and the cross neutralization response between the two different SARS-CoV strains gives a hint of the similar neutralizing epitopes, which provide stable bases for the development of inactivated SARS-CoV vaccines. 展开更多
关键词 ANIMALS antibodies viral Immunoglobulin G Neutralization Tests RABBITS Research Support Non-U.S. Gov't SARS Virus Vaccines Inactivated viral Vaccines
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