期刊文献+
共找到7篇文章
< 1 >
每页显示 20 50 100
Antigen epitopes of animal coronaviruses:a mini-review
1
作者 Mingjun Su Guanghui Zheng +1 位作者 Xiangwen Xu Houhui Song 《Animal Diseases》 CAS 2024年第1期19-26,共8页
Coronaviruses are widespread in nature and can infect mammals and poultry,making them a public health concern.Globally,prevention and control of emerging and re-emerging animal coronaviruses is a great challenge.The m... Coronaviruses are widespread in nature and can infect mammals and poultry,making them a public health concern.Globally,prevention and control of emerging and re-emerging animal coronaviruses is a great challenge.The mecha-nisms of virus-mediated immune responses have important implications for research on virus prevention and control.The antigenic epitope is a chemical group capable of stimulating the production of antibodies or sensitized lympho-cytes,playing an important role in antiviral immune responses.Thus,it can shed light on the development of diagnos-tic methods and novel vaccines.Here,we have reviewed advances in animal coronavirus antigenic epitope research,aiming to provide a reference for the prevention and control of animal and human coronaviruses. 展开更多
关键词 Animal coronavirus antigen epitope B-cell epitope T-cell epitope Immune responses Vaccines
下载PDF
Two nanobody‑based immunoassays to differentiate antibodies against genotype 1 and 2 porcine reproductive and respiratory syndrome virus
2
作者 Xu Chen Yueting Chang +11 位作者 Lu Zhang Xinyu Zhao Zhihan Li Zhijie Zhang Pinpin Ji Qingyuan Liu Jiakai Zhao Jiahong Zhu Baoyuan Liu Xinjie Wang Yani Sun Qin Zhao 《Animal Diseases》 CAS 2024年第2期98-114,共17页
Porcine reproductive and respiratory syndrome virus(PRRSV)infection causes significant economic loss to the global pig industry.Genotype 1 and 2 PRRSV(PRRSV-1 and-2)infections have been reported in China,Europe and Am... Porcine reproductive and respiratory syndrome virus(PRRSV)infection causes significant economic loss to the global pig industry.Genotype 1 and 2 PRRSV(PRRSV-1 and-2)infections have been reported in China,Europe and America.For accurate prevention,nanobodies were first used as diagnostic reagents for PRRSV typing.In this study three nanobodies targeting both PRRSV-1 and-2,two targeting PRRSV-1 and three targeting PRRSV-2,were screened and produced.To develop two competitive ELISAs(cELISAs),the g1-2-PRRSV-Nb3-HRP nanobody was chosen for the g1-2-cELISA,to detect common antibodies against PRRSV-1 and-2,and the g1-PRRSV-Nb136-HRP nanobody was chosen for the g1-cELISA,to detect anti-PRRSV-1 antibodies.The two cELISAs were developed using PRRSV-1-N protein as coating antigen,and the amounts for both were 100 ng/well.The optimized dilution of testing pig sera was 1:20,the optimized reaction times were 30 min,and the colorimetric reaction times were 15 min.Then,the cut-off values of the g1-2-cELISA and g1-cELISA were 26.6%and 35.6%,respectively.Both of them have high sensitivity,strong specificity,good repeatability,and stability.In addition,for the 1534 clinical pig sera,an agreement rate of 99.02%(Kappa values=0.97)was determined between the g1-2-cELISA and the commercial IDEXX ELISA kit.For the g1-cELSIA,it can specifically detect anti-PRRSV-1 antibodies in the clinical pig sera.Importantly,combining two nanobody-based cELISAs can differentially detect antibodies against PRRSV-1 and-2. 展开更多
关键词 PRRSV Competitive ELISA NANOBODY antigen epitope
下载PDF
An Indirect ELISA of Classical Swine Fever Virus Based on Quadruple Antigenic Epitope Peptide Expressed in E.coli 被引量:4
3
作者 Guo-zhen LIN Fu-ying ZHENG Ji-zhang ZHOU Xiao-an CAO Xiao-wei GONG Guang-hua WANG Chang-qing QIU 《Virologica Sinica》 SCIE CAS CSCD 2010年第1期71-76,共6页
In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating... In this study,a synthesized quadruple antigenic epitope gene region of the classical swine fever virus (CSFV)E2 glycoprotein was expressed in E.coli to a obtain target protein.This target protein was used as a coating antigen to establish an indirect ELISA for specifically detecting anti-CSFV antibodies in serum samples from pigs.The P/N cut-off value of this assay was 1.92 by receiver operating characteristic curve(ROC)analysis based on 30 negative sera and 80 positive samples.The test gave 97.5%sensitivity and 96.7%specificity compared with the indirect hemagglutination(IHA)test.The inter-assay and intra-assay coefficients of variation (CVs)for 16 sera were both≤6.8%.No cross-reactivity between the coating antigen and anti-bovine viral diarrhoea virus(BVDV)antibodies was observed. 展开更多
关键词 antigenic epitope Bovine viral diarrhoea virus (BVDV) Classical swine fever virus (CSFV) Expression Indirect ELISA
下载PDF
Prokaryotic Expression and Immunogenicity of Major Antigen Epitope of Rabbit Haemorrhagic Disease Virus VP60 被引量:1
4
作者 SUI Hui YANG Jin-sheng 《Animal Husbandry and Feed Science》 CAS 2010年第5期29-30,33,共3页
[ Objective] To investigate the immunogenicity of main antigen epitope of RHDV ( Rabbit haemorrhagic disease virus) VP60 expressed in a prokaryotic system. [ Method] The major antigen epitope gene of RHDV VP60 was a... [ Objective] To investigate the immunogenicity of main antigen epitope of RHDV ( Rabbit haemorrhagic disease virus) VP60 expressed in a prokaryotic system. [ Method] The major antigen epitope gene of RHDV VP60 was amplified by RT-PCR. It was cloned into pET-28b ( + ) and expressed in E. coli Rosetta strain. The recombinant protein was detected by Western blot. The pudfied recombinant protein was used to immunize rabbits in order to observe its immunogenicity. [ Result] Western blot analysis revealed a clear band at approximately 24.0 kDa. The purified recom- binant protein reacted with the purified RHDV in ELISA. [ Conclusion] The prokaryotically expressed main antigen epitope of RHDV VP60 shows good immunogenicity. 展开更多
关键词 Rabbit haemorrhagic disease virus VP60 Major antigen epitope IMMUNOGENICITY
下载PDF
Computational analysis of antigenic epitopes of avian influenza A (H7N9) viruses 被引量:6
5
作者 LIU Mi SONG TingRui +2 位作者 HUA Sha WU AiPing JIANG TaiJiao 《Science China(Life Sciences)》 SCIE CAS CSCD 2015年第7期687-693,共7页
Influenza virus can rapidly change its antigenicity, via mutation in the hemagglutinin(HA) protein, to evade host immunity. The emergence of the novel human-infecting avian H7N9 virus in China has caused widespread co... Influenza virus can rapidly change its antigenicity, via mutation in the hemagglutinin(HA) protein, to evade host immunity. The emergence of the novel human-infecting avian H7N9 virus in China has caused widespread concern. However, evolution of the antigenicity of this virus is not well understood. Here, we inferred the antigenic epitopes of the HA protein from all H7 viruses, based on the five well-characterized HA epitopes of the human H3N2 virus. By comparing the two major H7 phylogenetic lineages, i.e., the Eurasian lineage and the North American lineage, we found that epitopes A and B are more frequently mutated in the Eurasian lineage, while epitopes B and C are more frequently mutated in the North American lineage. Furthermore, we found that the novel H7N9 virus(derived from the Eurasian lineage) isolated in China in the year 2013, contains six frequently mutated sites on epitopes that include site 135, which is located in the receptor binding domain. This indicates that the novel H7N9 virus that infects human may already have been subjected to gradual immune pressure and receptor-binding variation. Our results not only provide insights into the antigenic evolution of the H7 virus but may also help in the selection of suitable vaccine strains. 展开更多
关键词 antigenic epitope antigenic evolution HEMAGGLUTININ influenza H7N9 virus
原文传递
Recent advances in synthetic carbohydrate-based human immunodeficiency virus vaccines 被引量:2
6
作者 Zhenyuan Wang Chunjun Qin +2 位作者 Jing Hu Xiaoqiang Guo Jian Yin 《Virologica Sinica》 SCIE CAS CSCD 2016年第2期110-117,共8页
An effective vaccine for human immunodeficiency virus(HIV) is urgently needed to prevent HIV infection and progression to acquired immune deficiency syndrome(AIDS). As glycosylation of viral proteins becomes better un... An effective vaccine for human immunodeficiency virus(HIV) is urgently needed to prevent HIV infection and progression to acquired immune deficiency syndrome(AIDS). As glycosylation of viral proteins becomes better understood, carbohydrate-based antiviral vaccines against special viruses have attracted much attention. Significant efforts in carbohydrate synthesis and immunogenicity research have resulted in the development of multiple carbohydrate-based HIV vaccines. This review summarizes recent advances in synthetic carbohydrate-based vaccines design strategies and the applications of these vaccines in the prevention of HIV. 展开更多
关键词 vaccines carbohydrate advances antiviral epitope vaccination antiretroviral antigenic prevention understood
原文传递
A multi-center study on genetic variations in the fusion protein of respiratory syncytial virus from children with Acute Lower Respiratory Tract Infections in China during 2017-2021
7
作者 Yiliang Fu Fei Li +15 位作者 Yun Zhu Luci Huang Qiuping Li Hanwen Zhang Lili Zhong Hailin Zhang Zheng-xiu Luo Gen Lu Jikui Deng Lingfeng Cao Ying Wu Rong Jin Lei Li Lili Xu Xiangpeng Chen Zhengde Xie 《Virologica Sinica》 SCIE CAS 2024年第5期727-736,共10页
Respiratory syncytial virus(RSV)is a significant cause of acute lower respiratory tract infection(ALRTI)in children underfive years of age.Between 2017 and 2021,396 complete sequences of the RSV F gene were obtained fro... Respiratory syncytial virus(RSV)is a significant cause of acute lower respiratory tract infection(ALRTI)in children underfive years of age.Between 2017 and 2021,396 complete sequences of the RSV F gene were obtained from 500 RSV-positive throat swabs collected from ten hospitals across nine provinces in China.In addition,151 sequences from China were sourced from GenBank and GISAID,making a total of 549 RSV F gene sequences subjected to analysis.Phylogenetic and genetic diversity analyses revealed that the RSV F genes circulating in China from 2017 to 2021 have remained relatively conserved,although some amino acids(AAs)have undergone changes.AA mutations with frequencies10%were identified at six sites and the p27 region:V384I(site I),N276S(site II),R213S(siteØ),and K124N(p27)for RSV A;F45L(site I),M152I/L172Q/S173 L/I185V/K191R(site V),and R202Q/I206M/Q209R(siteØ)for RSV B.Comparing mutational frequencies in RSV-F before and after 2020 revealed minor changes for RSV A,while the K191R,I206M,and Q209R frequencies increased by over 10%in RSV B.Notably,the nirsevimab-resistant mutation,S211N in RSV B,increased in frequency from 0%to 1.15%.Both representative strains aligned with the predicted RSV-F structures of their respective prototypes exhibited similar conformations,with low root-mean-square deviation values.These results could provide foundational data from China for the development of RSV mAbs and vaccines. 展开更多
关键词 Human respiratory syncytial virus(RSV) Children Fusion glycoprotein antigenic epitope Variation
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部