The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievem...The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved （including an estimate of the number of leucocyte surface molecules still to be discovered）, and how the field can best move forward.展开更多
BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain...BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain to be further explored.AIM The aim was to reveal the possible mechanisms by which hypoxia enhances the proliferation of hP-MSCs.METHODS A hypoxic cell incubator(2.5%O2)was used to mimic a hypoxic microenvironment.Cell counting kit-8 and 5-ethynyl-20-deoxyuridine incorporation assays were used to assay the proliferation of hP-MSCs.The cell cycle was profiled by flow cytometry.Transcriptome profiling of hP-MSCs under hypoxia was performed by RNA sequencing.CD99 mRNA expression was assayed by reverse transcription-polymerase chain reaction.Small interfering RNA-mediated hypoxia-inducible factor 1α(HIF-1α)or CD99 knockdown of hP-MSCs,luciferase reporter assays,and the ERK1/2 signaling inhibitor PD98059 were used in the mechanistic analysis.Protein expression was assayed by western blotting;immunofluorescence assays were conducted to evaluate changes in expression levels.RESULTS Hypoxia enhanced hP-MSC proliferation,increased the expression of cyclin E1,cyclin-dependent kinase 2,and cyclin A2,and decreased the expression of p21.Under hypoxia,CD99 expression was increased by HIF-1α.CD99-specific small interfering RNA or the ERK1/2 signaling inhibitor PD98059 abrogated the hypoxia-induced increase in cell proliferation.CONCLUSION Hypoxia promoted hP-MSCs proliferation in a manner dependent on CD99 regulation of the MAPK/ERK signaling pathway in vitro.展开更多
目的探讨LAT和CD99在T淋巴母细胞淋巴瘤(precursor T lymphoblastic lymphoma,T-LBL)中表达的价值。方法对37例T-LBL应用免疫组织化学EnVision二步法进行LAT和CD99标记。同时选取15例其他病例作为对照:3例B淋巴母细胞淋巴瘤,4例非特殊...目的探讨LAT和CD99在T淋巴母细胞淋巴瘤(precursor T lymphoblastic lymphoma,T-LBL)中表达的价值。方法对37例T-LBL应用免疫组织化学EnVision二步法进行LAT和CD99标记。同时选取15例其他病例作为对照:3例B淋巴母细胞淋巴瘤,4例非特殊类型外周T细胞淋巴瘤,3例结外鼻型NK/T细胞淋巴瘤,5例淋巴结反应性增生。结果37例T-LBL均表达LAT和CD99;4例外周T细胞淋巴瘤及3例鼻型结外NK/T细胞淋巴瘤弥漫表达LAT,不表达CD99;3例B淋巴母细胞淋巴瘤均表达CD99,但不表达LAT;5例反应性增生淋巴结的T细胞区LAT阳性,淋巴结皮、髓质区均不表达CD99。结论联合检测LAT和CD99有助于T-LBL的诊断和鉴别诊断。展开更多
文摘The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved （including an estimate of the number of leucocyte surface molecules still to be discovered）, and how the field can best move forward.
基金Stem Cell and Translational Research from the National Key Research and Development Program of China,No.2020YFA0113003National Natural Science Foundation of China, No. 81971756.
文摘BACKGROUND As human placenta-derived mesenchymal stem cells(hP-MSCs)exist in a physiologically hypoxic microenvironment,various studies have focused on the influence of hypoxia.However,the underlying mechanisms remain to be further explored.AIM The aim was to reveal the possible mechanisms by which hypoxia enhances the proliferation of hP-MSCs.METHODS A hypoxic cell incubator(2.5%O2)was used to mimic a hypoxic microenvironment.Cell counting kit-8 and 5-ethynyl-20-deoxyuridine incorporation assays were used to assay the proliferation of hP-MSCs.The cell cycle was profiled by flow cytometry.Transcriptome profiling of hP-MSCs under hypoxia was performed by RNA sequencing.CD99 mRNA expression was assayed by reverse transcription-polymerase chain reaction.Small interfering RNA-mediated hypoxia-inducible factor 1α(HIF-1α)or CD99 knockdown of hP-MSCs,luciferase reporter assays,and the ERK1/2 signaling inhibitor PD98059 were used in the mechanistic analysis.Protein expression was assayed by western blotting;immunofluorescence assays were conducted to evaluate changes in expression levels.RESULTS Hypoxia enhanced hP-MSC proliferation,increased the expression of cyclin E1,cyclin-dependent kinase 2,and cyclin A2,and decreased the expression of p21.Under hypoxia,CD99 expression was increased by HIF-1α.CD99-specific small interfering RNA or the ERK1/2 signaling inhibitor PD98059 abrogated the hypoxia-induced increase in cell proliferation.CONCLUSION Hypoxia promoted hP-MSCs proliferation in a manner dependent on CD99 regulation of the MAPK/ERK signaling pathway in vitro.
文摘目的探讨LAT和CD99在T淋巴母细胞淋巴瘤(precursor T lymphoblastic lymphoma,T-LBL)中表达的价值。方法对37例T-LBL应用免疫组织化学EnVision二步法进行LAT和CD99标记。同时选取15例其他病例作为对照:3例B淋巴母细胞淋巴瘤,4例非特殊类型外周T细胞淋巴瘤,3例结外鼻型NK/T细胞淋巴瘤,5例淋巴结反应性增生。结果37例T-LBL均表达LAT和CD99;4例外周T细胞淋巴瘤及3例鼻型结外NK/T细胞淋巴瘤弥漫表达LAT,不表达CD99;3例B淋巴母细胞淋巴瘤均表达CD99,但不表达LAT;5例反应性增生淋巴结的T细胞区LAT阳性,淋巴结皮、髓质区均不表达CD99。结论联合检测LAT和CD99有助于T-LBL的诊断和鉴别诊断。
