The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by establish...The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by established in-vitro agar diffusion method.The methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different polarities.The agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g(+and-)microbial strains.Antimicrobial potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial strains.The highest activity chloroform extract was analysed by column chromatography(CC)for the isolation of antimicrobial compounds.The structure of the isolated compounds was characterized based on 1D,2D NMR and MS spectra.In conclusion,purest compounds might be useful as a remedy for infectious diseases.展开更多
AIM:An antimicrobial validatory screening of puffer fish is done in such a way Arothron immaculatus a puffer fish skin and liver extracts were subjected for antimicrobial assay.METHODS:Antimicrobial screening assay wa...AIM:An antimicrobial validatory screening of puffer fish is done in such a way Arothron immaculatus a puffer fish skin and liver extracts were subjected for antimicrobial assay.METHODS:Antimicrobial screening assay was done in ten consecutive human pathogenic bacterial and fungal pathogens using the standard disc diffusion method.RESULTS:The results confirmed a positive test against most of the pathogens used.Maximum antimicrobial effect against Staphyloccocus aureus of 2.5 mm in liver extract and 9.8 mm of antibacterial effect against Vibrio cholera in skin extract is reported.There is no antifungal effect.CONCLUSION:This experiment confirms that puffer fish is a source of antimicrobial potence.展开更多
文摘The main aim of this research work was to isolate and characterize the antimicrobial compounds that can be extracted from the leaves of Dodonaea viscosa(D.viscosa)and to assess their antimicrobial potency by established in-vitro agar diffusion method.The methanol extract was liquefied and fractioned by using a separatory funnel and organic solvents having different polarities.The agar diffusion technique was used to assess the antimicrobial potency of extracts and pure compounds against 5 g(+and-)microbial strains.Antimicrobial potency results showed that all extracts and isolated pure compounds provided significant antimicrobial potency against the applied microbial strains.The highest activity chloroform extract was analysed by column chromatography(CC)for the isolation of antimicrobial compounds.The structure of the isolated compounds was characterized based on 1D,2D NMR and MS spectra.In conclusion,purest compounds might be useful as a remedy for infectious diseases.
基金supported by the Ministry of Earth Science and Ocean Development [MoES & OASTC],Government of India
文摘AIM:An antimicrobial validatory screening of puffer fish is done in such a way Arothron immaculatus a puffer fish skin and liver extracts were subjected for antimicrobial assay.METHODS:Antimicrobial screening assay was done in ten consecutive human pathogenic bacterial and fungal pathogens using the standard disc diffusion method.RESULTS:The results confirmed a positive test against most of the pathogens used.Maximum antimicrobial effect against Staphyloccocus aureus of 2.5 mm in liver extract and 9.8 mm of antibacterial effect against Vibrio cholera in skin extract is reported.There is no antifungal effect.CONCLUSION:This experiment confirms that puffer fish is a source of antimicrobial potence.
