investigate the role of cationic antimicrobial protein of Mr 37?kDa (CAP37) a neutrophil derived inflammatory mediator on endothelial cell function Data sources Endothelial cells used in this study were obtained from ...investigate the role of cationic antimicrobial protein of Mr 37?kDa (CAP37) a neutrophil derived inflammatory mediator on endothelial cell function Data sources Endothelial cells used in this study were obtained from human lung microvessels and rat aorta The latter was a kind gift of Dr Paula Grammas The mono mac 6 cell line used in this study was the generous gift of Dr H W Loms Ziegler Heitbrock Study selection and data extraction Endothelial cell proteins kinase C activity was determined by measuring calcium and phospholipid dependent phosphorylation of histone Endothelial cell migration was determined using Costar TM Transwell apparatus Cell surface expression of adhesion molecules, ICAM 1 and PECAM 1 was determined using flow cytometry RT PCR was used to amplify the CAP37 from endothelial cells treated with LPS Results We demonstrated that CAP37 which was originally identified as having potent antimicrobial activity and chemotactic activity for monocytes was capable of modulating endothelial cell functions CAP37 activated endothelial cell protein kinase C in a dose and time dependent fashion Importantly CAP37 increased the adhesive properties of the endothelium for monocytes CAP37 upregulated the well known adhesion molecules, ICAM 1 and PECAM 1 in a dose and time dependent manner In addition, CAP37 promoted endothelial cell migration Further investigations indicated that CAP37 was induced in endothelial cells in response to pro inflammatory cytokines such as tumor necrosis factor α and interleukin 1α as well as inflammatory mediators such as lipopolysaccharide Unstimulated endothelial cells did not constitutively express CAP37 The cDNA sequence of endothelial CAP37 was determined and found to be highly homologous to the sequence obtained for neutrophil derived CAP37 Conclusions Our studies strongly suggest that CAP37 plays a pivotal role in monocyte endothelial interactions and the transmigration of monocytes from the vasculature into extravascular展开更多
An antibacterial protein was isolated from the cultured mycelia of Cordyceps sinensis, and was designated as Cordyceps sinensis Antibacterial Protein (CSAP). CSAP was single-chained, with an apparent molecular mass ...An antibacterial protein was isolated from the cultured mycelia of Cordyceps sinensis, and was designated as Cordyceps sinensis Antibacterial Protein (CSAP). CSAP was single-chained, with an apparent molecular mass of 35 × 10^3 revealed by SDS-PAGE and a novel hydrophobic N-terminal sequence N-ALATQHGAP. The antimicrobial assays showed CSAP could inbibit the growth of Gram-positive and Gram-negative bacteria but no significant inhibition against fungi or yeasts. Further more, the antibacterial activity of CSAP was not bactericidal but bacteriostatic. It was the first time that an antibacterial protein was described in the Cordyceps species, which might involve in the chemical defense mechanism of the hosts.展开更多
Objective Cationic antimicrobial protein of 37 kDa(CAP37),a neutrophil-derived protein originally identified for its antimicrobial activity,is now known to have many regulatory effects on host cells.However,its role i...Objective Cationic antimicrobial protein of 37 kDa(CAP37),a neutrophil-derived protein originally identified for its antimicrobial activity,is now known to have many regulatory effects on host cells.However,its role in the pathogenesis of chronic obstructive pulmonary disease(COPD)has not been studied.We therefore investigated the expression of CAP37 in COPD and its effects on airway structural cells,including bronchial epithelial cells,smooth muscle cells,and fibroblasts.Methods CAP37 was detected in the lung tissue,sputum,and plasma of COPD patients and the control subjects,as well as in the neutrophils stimulated by cigarette smoke extract(CSE).BEAS-2B cells,human bronchial smooth muscle cells(HBSMCs),and MRC-5 cells were treated with CAP37 or an anti-CAP37 antibody plus CAP37.Interleukin(IL)-6 and IL-8 were detected in the BEAS-2B cells.The cell proliferation was analyzed in the HBSMCs.Collagens were also detected in the MRC-5 cells.Results The expression of CAP37 was increased in the lung tissue and sputum supernatant of the COPD patients compared with the control subjects.The sputum supernatant CAP37 levels were inversely correlated with the forced expiratory volume in the first second percentage predicted in COPD.CAP37 was induced by CSE stimulation in the peripheral blood neutrophils from healthy non-smokers.CAP37 induced expression of IL-6 and IL-8 in BEAS-2B cells,and collagen expression of lung fibroblasts(MRC-5 cells).However,CAP37 did not significantly alter the proliferation of the HBSMCs.Conclusion Our findings indicated that neutrophil-derived CAP37 may be involved in airway inflammation and fibrosis in COPD via affecting the bronchial epithelial cells,and fibroblasts,thus suggesting a possible role of CAP37 in the development and progression of COPD.展开更多
In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum ( Syd. ), which can cause sugarcane smut, the full-length TasA gene which encodes a protein ...In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum ( Syd. ), which can cause sugarcane smut, the full-length TasA gene which encodes a protein with broad-spectrum antimicrobial activity, was PCR-amplified from HAS, and cloned into pMD18-T vector. Sequence analysis indicated that the full-length TasA cloned from HAS consisted of 786 nucleotides, and shared 99% homology in nucleotide sequence with the TasA gene sequence published in Genbank (AJ871386.1). It was predicted that the molecular weight of TasA protein was about 28 kD. Base transitions or transversions ~curred at positions 104, 164, 169,250, 399,623 and 627, at the 2nd, 2nd, 3rd , 1st , 3rd, 2nd and 3rd bases of TasA codons. The mutations in the seven bases may cause the missense mutations of the polypeptide chain. Compared with the amino acid sequences of the TasA protein encoded by Bacillus aubtilis subsp, subtilis str. 168, mutations in two amino acids at positions 150 and 209 of the protein encoded by the cloned TasA gene were found, and as a result, an ala- nine was replaced with a threonine.展开更多
The COVID-19 caused by SARS-CoV-2 has resulted in millions of people being infected and thousands of deaths globally since November 2019.To date,no unique therapeutic agent has been developed to slow the progression o...The COVID-19 caused by SARS-CoV-2 has resulted in millions of people being infected and thousands of deaths globally since November 2019.To date,no unique therapeutic agent has been developed to slow the progression of this pandemic.Despite possessing antiviral traits the potential of bacteriocins to combat SARS-CoV-2 infection has not been fully investigated.This review summarizes the mechanisms by which bacteriocins can be manipulated and implemented as effective virus entry blockers with infection suppression potential properties to highly transmissible viruses through comprehensive immune modulations that are potentially effective against COVID-19.These antimicrobial peptides have been suggested as effective antiviral therapeutics and therapeutic supplements to prevent rapid virus transmission.This review also provides a new insight into the cellular and molecular alterations which have made SARS-CoV-2 self-modified with diversified infection patterns.In addition,the possible applications of antimicrobial peptides through both natural and induced mechanisms in infection prevention perspectives on changeable virulence cases are comprehensively analyzed.Specific attention is given to the antiviral mechanisms of the molecules along with their integrative use with synthetic biology and nanosensor technology for rapid detection.Novel bacteriocin based therapeutics with cutting-edge technologies might be potential substitutes for existing time-consuming and expensive approaches to fight this newly emerged global threat.展开更多
基金supported in part by an Established Investigata Grant from the American Heart Association and Public Health Service Grant AI28018 from the Natianal Institute of Allergy and Infectious Diseases
文摘investigate the role of cationic antimicrobial protein of Mr 37?kDa (CAP37) a neutrophil derived inflammatory mediator on endothelial cell function Data sources Endothelial cells used in this study were obtained from human lung microvessels and rat aorta The latter was a kind gift of Dr Paula Grammas The mono mac 6 cell line used in this study was the generous gift of Dr H W Loms Ziegler Heitbrock Study selection and data extraction Endothelial cell proteins kinase C activity was determined by measuring calcium and phospholipid dependent phosphorylation of histone Endothelial cell migration was determined using Costar TM Transwell apparatus Cell surface expression of adhesion molecules, ICAM 1 and PECAM 1 was determined using flow cytometry RT PCR was used to amplify the CAP37 from endothelial cells treated with LPS Results We demonstrated that CAP37 which was originally identified as having potent antimicrobial activity and chemotactic activity for monocytes was capable of modulating endothelial cell functions CAP37 activated endothelial cell protein kinase C in a dose and time dependent fashion Importantly CAP37 increased the adhesive properties of the endothelium for monocytes CAP37 upregulated the well known adhesion molecules, ICAM 1 and PECAM 1 in a dose and time dependent manner In addition, CAP37 promoted endothelial cell migration Further investigations indicated that CAP37 was induced in endothelial cells in response to pro inflammatory cytokines such as tumor necrosis factor α and interleukin 1α as well as inflammatory mediators such as lipopolysaccharide Unstimulated endothelial cells did not constitutively express CAP37 The cDNA sequence of endothelial CAP37 was determined and found to be highly homologous to the sequence obtained for neutrophil derived CAP37 Conclusions Our studies strongly suggest that CAP37 plays a pivotal role in monocyte endothelial interactions and the transmigration of monocytes from the vasculature into extravascular
基金Supported by the National Natural Science Foundation of China(39770200) the Natural Science Foundation of Hubei Province (2004ABA228)
文摘An antibacterial protein was isolated from the cultured mycelia of Cordyceps sinensis, and was designated as Cordyceps sinensis Antibacterial Protein (CSAP). CSAP was single-chained, with an apparent molecular mass of 35 × 10^3 revealed by SDS-PAGE and a novel hydrophobic N-terminal sequence N-ALATQHGAP. The antimicrobial assays showed CSAP could inbibit the growth of Gram-positive and Gram-negative bacteria but no significant inhibition against fungi or yeasts. Further more, the antibacterial activity of CSAP was not bactericidal but bacteriostatic. It was the first time that an antibacterial protein was described in the Cordyceps species, which might involve in the chemical defense mechanism of the hosts.
基金supported by grants from the National Natural Science Foundation of China(No.81670035NSFC)the COPD Research Programs of Beijing Bethune Charitable Foundation(No.BJ-RW2020015J).
文摘Objective Cationic antimicrobial protein of 37 kDa(CAP37),a neutrophil-derived protein originally identified for its antimicrobial activity,is now known to have many regulatory effects on host cells.However,its role in the pathogenesis of chronic obstructive pulmonary disease(COPD)has not been studied.We therefore investigated the expression of CAP37 in COPD and its effects on airway structural cells,including bronchial epithelial cells,smooth muscle cells,and fibroblasts.Methods CAP37 was detected in the lung tissue,sputum,and plasma of COPD patients and the control subjects,as well as in the neutrophils stimulated by cigarette smoke extract(CSE).BEAS-2B cells,human bronchial smooth muscle cells(HBSMCs),and MRC-5 cells were treated with CAP37 or an anti-CAP37 antibody plus CAP37.Interleukin(IL)-6 and IL-8 were detected in the BEAS-2B cells.The cell proliferation was analyzed in the HBSMCs.Collagens were also detected in the MRC-5 cells.Results The expression of CAP37 was increased in the lung tissue and sputum supernatant of the COPD patients compared with the control subjects.The sputum supernatant CAP37 levels were inversely correlated with the forced expiratory volume in the first second percentage predicted in COPD.CAP37 was induced by CSE stimulation in the peripheral blood neutrophils from healthy non-smokers.CAP37 induced expression of IL-6 and IL-8 in BEAS-2B cells,and collagen expression of lung fibroblasts(MRC-5 cells).However,CAP37 did not significantly alter the proliferation of the HBSMCs.Conclusion Our findings indicated that neutrophil-derived CAP37 may be involved in airway inflammation and fibrosis in COPD via affecting the bronchial epithelial cells,and fibroblasts,thus suggesting a possible role of CAP37 in the development and progression of COPD.
基金Supported by National Natural Science Foundation of China(31471555)Natural Science Foundation of Hainan Province(314120)Earmarked Fund for Modern Agro-industry Technology Research System of China(nycytx-24)
文摘In order to clarify the mechanism for the inhibitory action of the Bacillus subtilis strain HAS on Sporisorium scitamineum ( Syd. ), which can cause sugarcane smut, the full-length TasA gene which encodes a protein with broad-spectrum antimicrobial activity, was PCR-amplified from HAS, and cloned into pMD18-T vector. Sequence analysis indicated that the full-length TasA cloned from HAS consisted of 786 nucleotides, and shared 99% homology in nucleotide sequence with the TasA gene sequence published in Genbank (AJ871386.1). It was predicted that the molecular weight of TasA protein was about 28 kD. Base transitions or transversions ~curred at positions 104, 164, 169,250, 399,623 and 627, at the 2nd, 2nd, 3rd , 1st , 3rd, 2nd and 3rd bases of TasA codons. The mutations in the seven bases may cause the missense mutations of the polypeptide chain. Compared with the amino acid sequences of the TasA protein encoded by Bacillus aubtilis subsp, subtilis str. 168, mutations in two amino acids at positions 150 and 209 of the protein encoded by the cloned TasA gene were found, and as a result, an ala- nine was replaced with a threonine.
基金the authority of Research Publication Guardians(RPG,Government License No.05-060-06021)for providing logistic support and suggestions which were important to the successful completion of this review。
文摘The COVID-19 caused by SARS-CoV-2 has resulted in millions of people being infected and thousands of deaths globally since November 2019.To date,no unique therapeutic agent has been developed to slow the progression of this pandemic.Despite possessing antiviral traits the potential of bacteriocins to combat SARS-CoV-2 infection has not been fully investigated.This review summarizes the mechanisms by which bacteriocins can be manipulated and implemented as effective virus entry blockers with infection suppression potential properties to highly transmissible viruses through comprehensive immune modulations that are potentially effective against COVID-19.These antimicrobial peptides have been suggested as effective antiviral therapeutics and therapeutic supplements to prevent rapid virus transmission.This review also provides a new insight into the cellular and molecular alterations which have made SARS-CoV-2 self-modified with diversified infection patterns.In addition,the possible applications of antimicrobial peptides through both natural and induced mechanisms in infection prevention perspectives on changeable virulence cases are comprehensively analyzed.Specific attention is given to the antiviral mechanisms of the molecules along with their integrative use with synthetic biology and nanosensor technology for rapid detection.Novel bacteriocin based therapeutics with cutting-edge technologies might be potential substitutes for existing time-consuming and expensive approaches to fight this newly emerged global threat.