Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective b...Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective breeding of high resistance good tetraploid varieties of C. cinerariifolium. Method The SOD, POD and APX activities of each sample were determined. Every material should have 3 replicates which been determined and calculated mean value to analyze. Result The various kinds of antioxidase activities in protective enzyme system of tetraploid lines were increased generally in comparison with that of diploid control line. And there is certain relativity between the samples in field and in test-tube of these lines. Conclusion The antioxidase activities of each line in test-tube could be considered as reference index to screen high resistance good tetraploid varieties of C. cinerariifolium.展开更多
Poor adventitious root formation is a major obstacle in micropropagation and in conventional propagation. The effects of LaCl_3 on root growth and deferring the root ageing of peach plantlet in vitro were investigated...Poor adventitious root formation is a major obstacle in micropropagation and in conventional propagation. The effects of LaCl_3 on root growth and deferring the root ageing of peach plantlet in vitro were investigated. The results show that the optimum concentration of LaCl_3 (2.5 μmol·L -1) in the rooted medium can significantly increase the rooting rate, the root length and the fresh weight, promote the activities of superoxide dismutase (SOD); catalase (CAT) and peroxidase (POD) and decrease the production rate of O_2 ·-, the malondialdehyde (MDA) contents and the plasma membrane permeability. There are important theory meaning and practical value in applying LaCl_3 in the rooting medium to raise the rate of rooting and transplant for stone fruit tree.展开更多
文摘Objective The SOD, POD and APX enzyme activities of tetraploid lines and diploid control line of Chrysanthemum cinerariifolium in test-tube and field were determined in order to provide a reference for the selective breeding of high resistance good tetraploid varieties of C. cinerariifolium. Method The SOD, POD and APX activities of each sample were determined. Every material should have 3 replicates which been determined and calculated mean value to analyze. Result The various kinds of antioxidase activities in protective enzyme system of tetraploid lines were increased generally in comparison with that of diploid control line. And there is certain relativity between the samples in field and in test-tube of these lines. Conclusion The antioxidase activities of each line in test-tube could be considered as reference index to screen high resistance good tetraploid varieties of C. cinerariifolium.
文摘Poor adventitious root formation is a major obstacle in micropropagation and in conventional propagation. The effects of LaCl_3 on root growth and deferring the root ageing of peach plantlet in vitro were investigated. The results show that the optimum concentration of LaCl_3 (2.5 μmol·L -1) in the rooted medium can significantly increase the rooting rate, the root length and the fresh weight, promote the activities of superoxide dismutase (SOD); catalase (CAT) and peroxidase (POD) and decrease the production rate of O_2 ·-, the malondialdehyde (MDA) contents and the plasma membrane permeability. There are important theory meaning and practical value in applying LaCl_3 in the rooting medium to raise the rate of rooting and transplant for stone fruit tree.
