Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not clear.An experiment was condu...Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not clear.An experiment was conducted to investigate the effects of dietary graded levels of CEO and its combination with BLF on the growth performance,immune responses,antioxidative ability,and intestinal morphology and microbiota of broilers fed a corn-soybean meal basal diet.A total of 576 1-d-old Arbor Acres commercial male broilers were randomly allotted to 9 treatments with 8 replicates of 8 birds each in a completely randomized design.Birds were fed on a basal corn-soybean meal diet(control,without plant extracts and antibiotics),or the basal diet supplemented with 50 mg of aureomycin kg^-1,50,100,200,400,or 800 mg of CEO kg^-1,a combination of 100 mg of CEO and 16.7 mg of BLF kg^-1,or a combination of 200 mg of CEO kg^-1 and 33.3 mg of BLF kg^-1 for 42 d.Dietary treatment affected(P<0.05)the serum immune globulin M(IgM)contents on d 42,liver malondialdehyde(MDA)contents on d 21,duodenal crypt depth on d 42,relative abundance of Lactobacillus in the cecal contents on d 21,and relative abundances of Escherichia coli and Bifidobacterium in the cecal contents on d 42,but had no effect(P>0.16)on all other measured indices.The addition of 400 mg of CEO kg^-1 or a combination of 200 mg CEO kg^-1 and 33.3 mg BLF kg^-1 increased(P<0.02)serum IgM contents on d 42.Dietary supplementation with 100 or 200 mg CEO kg^-1,or 50 mg aureomycin kg^-1 decreased(P<0.003)liver MDA contents on d 21.In addition,the supplement of 100 mg CEO kg^-1 increased(P<0.002)the Lactobacillus relative abundance in caecum on d 21 and Bifidobacterium relative abundance in caecum on d 42,and decreased(P<0.0001)E.coli relative abundance in caecum on d 42.The results indicated that dietary supplementation with CEO,an alternative to aureomycin,improved the immune status,antioxidantative ability and cecal microbiota of broilers,and dietary supplementation with the combinations of CEO and BLF did not exhibit further effects.Dietary supplementation with 100 mg CEO kg^-1 is beneficial for broilers fed a corn-soybean meal basal diet.展开更多
This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres...This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg^–1. The results showed that broilers fed the Se-supplemented diets had higher(P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase(GSH-Px) activities in breast and thigh muscles on d 42, and lower(P<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde(MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher(P<0.05) p H value and lower(P<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg^–1 had lower(P<0.05) shear force in thigh muscle and higher(P<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg^–1. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg^–1 had higher(P<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg^–1, but no differences(P<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg^–1. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antioxidative ability of broilers;and the Se from Se yeast was more effective than the Se from sodium selenite in improving meat quality of broilers.展开更多
Objective To determine the impact of passive smoking and the protective effect of antioxidants such as vitamin E and quercetin on learning and memory ability of mouse offsprings. Methods A passive smoking model of pre...Objective To determine the impact of passive smoking and the protective effect of antioxidants such as vitamin E and quercetin on learning and memory ability of mouse offsprings. Methods A passive smoking model of pregnant mice was established. Learning and memory ability was evaluated by the water maze test and long term potentiation （LTP）. Nitric oxide （NO）, content, nitric oxide synthase （NOS）, acetylcholinesteras （Ache） activity in brain, vitamin E concentration, and reactive oxygen species （ROS） in serum were determined. The latency period （the time during which the mice swim from the starting position to the ending position） and errors （the number of mice entering the blind end） in control and antioxidant intervention groups were compared with those in the smoke exposure group after 6 days. Results The latency period as well as errors in the air, control diet, tobacco smoke （TS）, and vitamin E diet groups were decreased significantly as compared with the TS and control diet groups （P〈O.05）. LTP was restrained in the TS and control diet groups. LTP in all the antioxidant diet groups was significantly increased compared with the TS and control diet groups. In addition, NOS and acetylcholinesteras （Ache） activitiy was significantly higher in the TS and control diet groups than in the air and control diet group. NO content was not significantly different among the different groups, and significantly lower in the TS and vitamin E diet groups than in the TS group, control diet group, quercetin diet group, and mixture diet group （P〈0.05）. Vitamin E concentration and ROS activity in serum were correlated with the outcome of water maze and LTP. Conclusion Passive smoking reduces LTP formation by disturbing the hippocampus function of mice, by decreasing NOS （especially vitamin E） partially improve the learning and memory smoke during pregnancy. and Ache activity and increasing NO content. Antioxidants ability of offsprings whose mothers are exposed to tobacco展开更多
Here, we investigated the effect of dietary arginine（Arg） supplementation on innate immunity and the antioxidant ability of broiler chickens. The experiment was designed as a single-factorial arrangement（n=8 cages/...Here, we investigated the effect of dietary arginine（Arg） supplementation on innate immunity and the antioxidant ability of broiler chickens. The experiment was designed as a single-factorial arrangement（n=8 cages/treatment, six birds/cage）, and we used four dietary Arg concentrations（10.0, 15.0, 20.0 or 25.0 g kg–1）. On day 21, the birds were killed to obtain spleen, cecal tonsil and liver samples to determine the gene expression and antioxidant characteristics. Increasing the Arg concentration linearly decreased（P0.05） the m RNA expression of splenic interleukin-18（IL-18） and tumor necrosis factor-α（TNF-α）. Dietary Arg supplementation quadratically decreased（P0.05） the expression of interleukin-1b（IL-1b） and interferon-γ（IFN-γ） m RNA in the spleen. Increasing Arg concentrations linearly and quadratically reduced the expression of IL-18 m RNA in the spleen. Meanwhile, increasing dietary Arg supplementation linearly and quadratically increased the lymphotactin m RNA（P0.05） expression, and linearly increased the macrophage inflammatory protein-1β（MIP-1β） and toll-like receptor 15（TLR15） m RNA expression in the cecal tonsils. Dietary Arg supplementation linearly（P0.05） increased the glutathione peroxidase（GSH-Px）, catalase（CAT）, and lysozyme（LZM） activities in the liver. However, the malondialdehyde（MDA） activity in the liver was not influenced by the dietary Arg concentration（P0.05）. No significant（P0.05） effect was found on the activity of superoxide dismutase（SOD） in the liver. Thus high levels of Arg supplementation（20.0 g kg^（–1）） may potentially suppress the innate immunity of broiler chickens, and dietary Arg supplementation enhances the antioxidant activity in broiler chickens.展开更多
基金supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201403047)the Special Funds of CAAS for Distinguished Scientists, China+2 种基金the earmarked fund for China Agriculture Research System (CARS-41)the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS08)the Key Agricultural Science and Technology Program of Datong, Shanxi, China (2016047)
文摘Cinnamon essential oil(CEO)and its combination with bamboo leaf flavonoid(BLF)have been shown to exhibit an additive antibacterial effect in vitro,but their functions in broilers were not clear.An experiment was conducted to investigate the effects of dietary graded levels of CEO and its combination with BLF on the growth performance,immune responses,antioxidative ability,and intestinal morphology and microbiota of broilers fed a corn-soybean meal basal diet.A total of 576 1-d-old Arbor Acres commercial male broilers were randomly allotted to 9 treatments with 8 replicates of 8 birds each in a completely randomized design.Birds were fed on a basal corn-soybean meal diet(control,without plant extracts and antibiotics),or the basal diet supplemented with 50 mg of aureomycin kg^-1,50,100,200,400,or 800 mg of CEO kg^-1,a combination of 100 mg of CEO and 16.7 mg of BLF kg^-1,or a combination of 200 mg of CEO kg^-1 and 33.3 mg of BLF kg^-1 for 42 d.Dietary treatment affected(P<0.05)the serum immune globulin M(IgM)contents on d 42,liver malondialdehyde(MDA)contents on d 21,duodenal crypt depth on d 42,relative abundance of Lactobacillus in the cecal contents on d 21,and relative abundances of Escherichia coli and Bifidobacterium in the cecal contents on d 42,but had no effect(P>0.16)on all other measured indices.The addition of 400 mg of CEO kg^-1 or a combination of 200 mg CEO kg^-1 and 33.3 mg BLF kg^-1 increased(P<0.02)serum IgM contents on d 42.Dietary supplementation with 100 or 200 mg CEO kg^-1,or 50 mg aureomycin kg^-1 decreased(P<0.003)liver MDA contents on d 21.In addition,the supplement of 100 mg CEO kg^-1 increased(P<0.002)the Lactobacillus relative abundance in caecum on d 21 and Bifidobacterium relative abundance in caecum on d 42,and decreased(P<0.0001)E.coli relative abundance in caecum on d 42.The results indicated that dietary supplementation with CEO,an alternative to aureomycin,improved the immune status,antioxidantative ability and cecal microbiota of broilers,and dietary supplementation with the combinations of CEO and BLF did not exhibit further effects.Dietary supplementation with 100 mg CEO kg^-1 is beneficial for broilers fed a corn-soybean meal basal diet.
基金financially supported by the National Natural Science Foundation of China (31601956)the Agricultural Science and Technology Innovation Program,China (ASTIP-IAS09)+1 种基金the earmarked fund for the China Agriculture Research System (CARS-41)the earmarked fund for Hebei Chicken Innovation Team of Modern Agro-industry Technology Research System,China (HBCT2018150203 and HBCT2018150206)。
文摘This experiment was conducted to investigate the effects of selenium(Se) source and level on growth performance, carcass traits, antioxidative ability and meat quality of broilers. A total of 320 one-d-old Arbor Acres commercial broilers were randomly assigned to 1 of 5 treatments with 8 replicates in a completely randomized design involving a 2×2 factorial arrangement of treatments plus one Se-unsupplemented basal diet control for 42 d. The two Se sources were sodium selenite and Se yeast, and the two supplemental Se levels were 0.20 and 0.40 mg Se kg^–1. The results showed that broilers fed the Se-supplemented diets had higher(P<0.05) average daily gain and average daily feed intake from 22 to 42 d of age, eviscerated yield and abdominal fat percentages, Se concentrations and glutathione peroxidase(GSH-Px) activities in breast and thigh muscles on d 42, and lower(P<0.05) feed/gain from 1 to 21 and 22 to 42 d of age, mortality from 22 to 42 d of age and malondialdehyde(MDA) concentration in thigh muscle on d 42 than those fed the control diet. Broilers fed the diets supplemented with Se yeast had higher(P<0.05) p H value and lower(P<0.05) shear force in thigh muscle than those fed the diets supplemented with sodium selenite. Additionally, broilers fed the diets supplemented with 0.40 mg Se kg^–1 had lower(P<0.05) shear force in thigh muscle and higher(P<0.05) GSH-Px activities in breast and thigh muscles than those fed the diets supplemented with 0.20 mg Se kg^–1. Furthermore, broilers fed the diet supplemented with Se yeast at 0.40 mg Se kg^–1 had higher(P<0.05) Se concentrations in breast and thigh muscles than those fed the diet supplemented with Se yeast at 0.20 mg Se kg^–1, but no differences(P<0.05) were observed in these indices of broilers fed the diets supplemented with sodium selenite between 0.20 and 0.40 mg Se kg^–1. The results from the present study indicated that supplemental Se could increase the growth performance, muscle Se concentration and antioxidative ability of broilers;and the Se from Se yeast was more effective than the Se from sodium selenite in improving meat quality of broilers.
文摘Objective To determine the impact of passive smoking and the protective effect of antioxidants such as vitamin E and quercetin on learning and memory ability of mouse offsprings. Methods A passive smoking model of pregnant mice was established. Learning and memory ability was evaluated by the water maze test and long term potentiation （LTP）. Nitric oxide （NO）, content, nitric oxide synthase （NOS）, acetylcholinesteras （Ache） activity in brain, vitamin E concentration, and reactive oxygen species （ROS） in serum were determined. The latency period （the time during which the mice swim from the starting position to the ending position） and errors （the number of mice entering the blind end） in control and antioxidant intervention groups were compared with those in the smoke exposure group after 6 days. Results The latency period as well as errors in the air, control diet, tobacco smoke （TS）, and vitamin E diet groups were decreased significantly as compared with the TS and control diet groups （P〈O.05）. LTP was restrained in the TS and control diet groups. LTP in all the antioxidant diet groups was significantly increased compared with the TS and control diet groups. In addition, NOS and acetylcholinesteras （Ache） activitiy was significantly higher in the TS and control diet groups than in the air and control diet group. NO content was not significantly different among the different groups, and significantly lower in the TS and vitamin E diet groups than in the TS group, control diet group, quercetin diet group, and mixture diet group （P〈0.05）. Vitamin E concentration and ROS activity in serum were correlated with the outcome of water maze and LTP. Conclusion Passive smoking reduces LTP formation by disturbing the hippocampus function of mice, by decreasing NOS （especially vitamin E） partially improve the learning and memory smoke during pregnancy. and Ache activity and increasing NO content. Antioxidants ability of offsprings whose mothers are exposed to tobacco
基金supported by the Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences(CAAS)supported by the National Key Technology R&D Program of China(2012BAD39B01)the Agricultural Science and Technology Innovation Program of CAAS(ASTIP-IAS07)in China
文摘Here, we investigated the effect of dietary arginine（Arg） supplementation on innate immunity and the antioxidant ability of broiler chickens. The experiment was designed as a single-factorial arrangement（n=8 cages/treatment, six birds/cage）, and we used four dietary Arg concentrations（10.0, 15.0, 20.0 or 25.0 g kg–1）. On day 21, the birds were killed to obtain spleen, cecal tonsil and liver samples to determine the gene expression and antioxidant characteristics. Increasing the Arg concentration linearly decreased（P0.05） the m RNA expression of splenic interleukin-18（IL-18） and tumor necrosis factor-α（TNF-α）. Dietary Arg supplementation quadratically decreased（P0.05） the expression of interleukin-1b（IL-1b） and interferon-γ（IFN-γ） m RNA in the spleen. Increasing Arg concentrations linearly and quadratically reduced the expression of IL-18 m RNA in the spleen. Meanwhile, increasing dietary Arg supplementation linearly and quadratically increased the lymphotactin m RNA（P0.05） expression, and linearly increased the macrophage inflammatory protein-1β（MIP-1β） and toll-like receptor 15（TLR15） m RNA expression in the cecal tonsils. Dietary Arg supplementation linearly（P0.05） increased the glutathione peroxidase（GSH-Px）, catalase（CAT）, and lysozyme（LZM） activities in the liver. However, the malondialdehyde（MDA） activity in the liver was not influenced by the dietary Arg concentration（P0.05）. No significant（P0.05） effect was found on the activity of superoxide dismutase（SOD） in the liver. Thus high levels of Arg supplementation（20.0 g kg^（–1）） may potentially suppress the innate immunity of broiler chickens, and dietary Arg supplementation enhances the antioxidant activity in broiler chickens.
