Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as hav...Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as having stable angina pectoris (SAP) (23 cases), unstable angina pectoris (UAP) (23 cases) or myocardial infarction (MI) (61 cases) were prospectively evaluated. DNA was obtained from the 107 patients and 50 controls. In order to determine the Apo AI genotypes at two polymorphic sites (G/A at -75 bp, and C/T at+83 bp), DNA was PCR amplified and digested with MspI. Results The frequency of carriers of the rare allele at the - 75 bp site (M1-) was 0.49 in cases and 0.30 in controls (P<0. 05). The frequencies of the M1-allele among patients with SAP, UAP, MI and controls were 0. 37 (vs. controls, P > 0. 05), 0.54 (vs. controls, P < 0.05), 0.52 (vs. controls, P<0. 05) and 0. 30, respectively. The frequencies for carriers of the rare allele at the + 83bp polymorphism (M2) were observed among patients with SAP (0. 09, vs. controls, P > 0.05), UAP (0.11, vs. controls, P>0.05) or MI (0. 12, vs. controls, P>0. 05) and controls (0. 12). There was an slightly increase in the frequency of the Ml - allele in patients with SAP to UAP or MI (0. 37 vs. 0. 54 vs. 0. 52; all P>0. 05) and Ml polymorphism as a risk factor for CAD ( OR = 3. 74, P < 0. 05). In the + 83bp polymorphism there was no difference in the allelelic frequencies in cases and controls (0. 11 vs. 0. 12; P > 0. 05). There was no significantdifference in the frequency of the M2 - allele in patients with SAP to UAP or MI (0.09 vs. 0. 11 vs. 0. 12; all P>0. 05) and M2 polymorphism not as a factor for CAD (OR=0.80, P>0. 05).Plasma lipoprotein values in patients with the allele M1-and M2 - had no different levels than those homozygous for the M1+and M2+(P>0.05). Conclusion Ml polymorphism (M1 - ) may be as a risk factor for CAD and M2 polymorphism (M2 - ) not as a factor for CAD in Chinese Xinjiang Uygur and Han population.展开更多
To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this...To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this study, 742 healthy Yi and Han subjects all above 15 years old formed the total population which was divided into three groups: the Yi farmer group, the Yi emigrant group and the Han resident group All estimates of plasma lipids and apolipoproteins were performed using an auto analyzer Genetic DNA was prepared from the blood clots using the Triton X 100 lysis technique Amplification of a 432?bp fragment of the apoAI gene promoter was performed using PCR followed by restriction digestion, electrophoresis and identification of the genotypes involved Results The samples were divided on the basis of alcohol consumption: non drinkers, 1-25?g/day, 26-75?g/day and >75?g/day Comparing the four alcohol consumption groups, plasma HDLC and apoAI levels were increased as the alcohol consumption increased with no evidence of threshold effects in the Yi farmers and the Han people groups A similar association was found in the Yi emigrant group, but was not statistically significant The frequencies of the A allele in the three populations were similar, and no significant difference of lipid and apolipoprotein levels was found between subjects with and without the A allele in the three populations But, in Han and Yi emigrant samples, the drinkers with the GG genotype had higher plasma HDLC and apoAI levels than non drinkers with the same genotype, while the drinkers with the A allele had lower plasma HDLC and apoAI levels than drinkers without the A allele Non drinkers with the A allele had higher levels of apoAI than non drinkers with GG genotypes It was estimated that 18% of the variability of plasma apoAI level could be explained by the G to A polymorphism in non drinkers of Yi emigrants ( F =8 94, P <0 01) Conclusions The present data suggest that moderate alcohol consumption and the G to A substitution could lower the risk of coronary heart disease (CHD), but the beneficial effects of one will be negated by the other展开更多
It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High ...It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High density lipoprotein is capable of delivering cholesterol from the peripheral tissues to the liver for disposal and therefore prevent the accumulation of cholesterol in the artery wall. Apolipoprotein AI (apoAI) is the major polypeptide of high density lipoproteins and seems to play an important role in this cholesterol transport.展开更多
文摘Objective To analyze the relationship between polymorphism at the Apolipoprotein AI (Apo AI) gene and the risk for coronary artery disease. Methods A total of 107 patients (mean age 56 ±11 years) diagnosed as having stable angina pectoris (SAP) (23 cases), unstable angina pectoris (UAP) (23 cases) or myocardial infarction (MI) (61 cases) were prospectively evaluated. DNA was obtained from the 107 patients and 50 controls. In order to determine the Apo AI genotypes at two polymorphic sites (G/A at -75 bp, and C/T at+83 bp), DNA was PCR amplified and digested with MspI. Results The frequency of carriers of the rare allele at the - 75 bp site (M1-) was 0.49 in cases and 0.30 in controls (P<0. 05). The frequencies of the M1-allele among patients with SAP, UAP, MI and controls were 0. 37 (vs. controls, P > 0. 05), 0.54 (vs. controls, P < 0.05), 0.52 (vs. controls, P<0. 05) and 0. 30, respectively. The frequencies for carriers of the rare allele at the + 83bp polymorphism (M2) were observed among patients with SAP (0. 09, vs. controls, P > 0.05), UAP (0.11, vs. controls, P>0.05) or MI (0. 12, vs. controls, P>0. 05) and controls (0. 12). There was an slightly increase in the frequency of the Ml - allele in patients with SAP to UAP or MI (0. 37 vs. 0. 54 vs. 0. 52; all P>0. 05) and Ml polymorphism as a risk factor for CAD ( OR = 3. 74, P < 0. 05). In the + 83bp polymorphism there was no difference in the allelelic frequencies in cases and controls (0. 11 vs. 0. 12; P > 0. 05). There was no significantdifference in the frequency of the M2 - allele in patients with SAP to UAP or MI (0.09 vs. 0. 11 vs. 0. 12; all P>0. 05) and M2 polymorphism not as a factor for CAD (OR=0.80, P>0. 05).Plasma lipoprotein values in patients with the allele M1-and M2 - had no different levels than those homozygous for the M1+and M2+(P>0.05). Conclusion Ml polymorphism (M1 - ) may be as a risk factor for CAD and M2 polymorphism (M2 - ) not as a factor for CAD in Chinese Xinjiang Uygur and Han population.
文摘To investigate the influence on plasma lipid levels of alcohol and a common polymorphism in the human apolipoprotein AI gene promoter at a position 75?bp upstream of the transcription start site Methods For this study, 742 healthy Yi and Han subjects all above 15 years old formed the total population which was divided into three groups: the Yi farmer group, the Yi emigrant group and the Han resident group All estimates of plasma lipids and apolipoproteins were performed using an auto analyzer Genetic DNA was prepared from the blood clots using the Triton X 100 lysis technique Amplification of a 432?bp fragment of the apoAI gene promoter was performed using PCR followed by restriction digestion, electrophoresis and identification of the genotypes involved Results The samples were divided on the basis of alcohol consumption: non drinkers, 1-25?g/day, 26-75?g/day and >75?g/day Comparing the four alcohol consumption groups, plasma HDLC and apoAI levels were increased as the alcohol consumption increased with no evidence of threshold effects in the Yi farmers and the Han people groups A similar association was found in the Yi emigrant group, but was not statistically significant The frequencies of the A allele in the three populations were similar, and no significant difference of lipid and apolipoprotein levels was found between subjects with and without the A allele in the three populations But, in Han and Yi emigrant samples, the drinkers with the GG genotype had higher plasma HDLC and apoAI levels than non drinkers with the same genotype, while the drinkers with the A allele had lower plasma HDLC and apoAI levels than drinkers without the A allele Non drinkers with the A allele had higher levels of apoAI than non drinkers with GG genotypes It was estimated that 18% of the variability of plasma apoAI level could be explained by the G to A polymorphism in non drinkers of Yi emigrants ( F =8 94, P <0 01) Conclusions The present data suggest that moderate alcohol consumption and the G to A substitution could lower the risk of coronary heart disease (CHD), but the beneficial effects of one will be negated by the other
基金Project supported by the National Natural Science Foundation of China.
文摘It has been suggested by many researchers that the dangers of developing atheroclerosis and the coronary heart disease in human race are closely correlated with the amount of high density lipoproteins in plasma. High density lipoprotein is capable of delivering cholesterol from the peripheral tissues to the liver for disposal and therefore prevent the accumulation of cholesterol in the artery wall. Apolipoprotein AI (apoAI) is the major polypeptide of high density lipoproteins and seems to play an important role in this cholesterol transport.
