目的探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo AⅠ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系。方法应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人A...目的探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo AⅠ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系。方法应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点进行限制性片段长度多态性(restriction fragment length polymorphisms,RFLP)分析。结果肝内胆管结石组中A1A1、A1A2、A2A2基因型频率分别为0、4.7%、95.3%,正常对照组中A1A1、A1A2、A2A2基因型频率分别为0、10.0%、90.0%。肝内胆管结石组和正常对照组各基因型频率的比较(χ2=1.599,P=0.206)和等位基因频率的比较(χ2=1.531,P=0.216)差异均无统计学意义。结论 Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点多态性可能不是肝内胆管结石的危险因素。展开更多
目的探讨HDL脂质转运蛋白ATP结合盒转运体A1(ATP-binding cassette transporter A1,ABCA1)、载脂蛋白A1(apolipoprotein A1,ApoA1)和吸收蛋白B类1型清道夫受体(scavenger receptor class B type 1,SR-B1)是否在Ⅰ型子宫内膜样癌(endomet...目的探讨HDL脂质转运蛋白ATP结合盒转运体A1(ATP-binding cassette transporter A1,ABCA1)、载脂蛋白A1(apolipoprotein A1,ApoA1)和吸收蛋白B类1型清道夫受体(scavenger receptor class B type 1,SR-B1)是否在Ⅰ型子宫内膜样癌(endometrial carcinoma,EC)中存在表达失衡。方法选取80例Ⅰ型EC和47例对照增生改变子宫内膜组织,采用免疫组化EnVision两步法检测ABCA1和ApoA1的表达;分析Ⅰ型EC中ABCA1、ApoA1与SR-B1、ER、PR蛋白表达的相关性及与临床病理特征的关系。结果(1)Ⅰ型EC中ABCA1阳性率为86.1%(62/72),低于对照增生改变子宫内膜组织(95.5%,42/44),差异有统计学意义(P<0.05);Ⅰ型EC组织中ApoA1阳性率为63.8%(44/69),高于对照增生改变子宫内膜组织(53.3%,23/43),差异无统计学意义(P>0.05);(2)在Ⅰ型EC中,ABCA1与SR-B1、ApoA1、ER之间呈负相关;ApoA1与PR之间呈正相关;(3)Ⅰ型EC中ABCA1和ApoA1蛋白表达与临床病理特征无明显相关性(P>0.05)。结论与对照增生改变子宫内膜组织相比,Ⅰ型EC中可能存在HDL转运蛋白ABCA1和HDL吸收蛋白SR-B1的表达失衡。展开更多
To identify, clone ,sequence and highly express the mature peptide gene of ApoA Ⅰ, total RNA was prepared from human fetal liver tissue. cDNA fragment encoding human ApoA Ⅰ was amplified by RT-PCR using specific pri...To identify, clone ,sequence and highly express the mature peptide gene of ApoA Ⅰ, total RNA was prepared from human fetal liver tissue. cDNA fragment encoding human ApoA Ⅰ was amplified by RT-PCR using specific primers, and then was inserted in pGEM-T vector. DNA sequencing indicates that the fragment is 729 base pairs in length and has 100% nucleotide homology with that of reported ApoA Ⅰ cDNA gene previously. The ApoA Ⅰ gene was cloned into pGEX 5X-1.The recombinant protein was expressed in E.coli DH5α, purified by glutathione-Sepharose 4B affinity chromatography and confirmed by SDS-PAGE. It was shown that the recombinant ApoA Ⅰ was expressed in E.coli, and the target protein amounted to 36% of total bacteria proteins. Cholesteryl ester transfer experiment showed that the recombinant ApoA Ⅰ was capable of promoting transfer of CE from HDL to LDL. Western blotting showed that the protein could react specifically with anti-ApoA Ⅰ antibodies.展开更多
文摘目的探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo AⅠ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系。方法应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点进行限制性片段长度多态性(restriction fragment length polymorphisms,RFLP)分析。结果肝内胆管结石组中A1A1、A1A2、A2A2基因型频率分别为0、4.7%、95.3%,正常对照组中A1A1、A1A2、A2A2基因型频率分别为0、10.0%、90.0%。肝内胆管结石组和正常对照组各基因型频率的比较(χ2=1.599,P=0.206)和等位基因频率的比较(χ2=1.531,P=0.216)差异均无统计学意义。结论 Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点多态性可能不是肝内胆管结石的危险因素。
文摘目的探讨HDL脂质转运蛋白ATP结合盒转运体A1(ATP-binding cassette transporter A1,ABCA1)、载脂蛋白A1(apolipoprotein A1,ApoA1)和吸收蛋白B类1型清道夫受体(scavenger receptor class B type 1,SR-B1)是否在Ⅰ型子宫内膜样癌(endometrial carcinoma,EC)中存在表达失衡。方法选取80例Ⅰ型EC和47例对照增生改变子宫内膜组织,采用免疫组化EnVision两步法检测ABCA1和ApoA1的表达;分析Ⅰ型EC中ABCA1、ApoA1与SR-B1、ER、PR蛋白表达的相关性及与临床病理特征的关系。结果(1)Ⅰ型EC中ABCA1阳性率为86.1%(62/72),低于对照增生改变子宫内膜组织(95.5%,42/44),差异有统计学意义(P<0.05);Ⅰ型EC组织中ApoA1阳性率为63.8%(44/69),高于对照增生改变子宫内膜组织(53.3%,23/43),差异无统计学意义(P>0.05);(2)在Ⅰ型EC中,ABCA1与SR-B1、ApoA1、ER之间呈负相关;ApoA1与PR之间呈正相关;(3)Ⅰ型EC中ABCA1和ApoA1蛋白表达与临床病理特征无明显相关性(P>0.05)。结论与对照增生改变子宫内膜组织相比,Ⅰ型EC中可能存在HDL转运蛋白ABCA1和HDL吸收蛋白SR-B1的表达失衡。
文摘To identify, clone ,sequence and highly express the mature peptide gene of ApoA Ⅰ, total RNA was prepared from human fetal liver tissue. cDNA fragment encoding human ApoA Ⅰ was amplified by RT-PCR using specific primers, and then was inserted in pGEM-T vector. DNA sequencing indicates that the fragment is 729 base pairs in length and has 100% nucleotide homology with that of reported ApoA Ⅰ cDNA gene previously. The ApoA Ⅰ gene was cloned into pGEX 5X-1.The recombinant protein was expressed in E.coli DH5α, purified by glutathione-Sepharose 4B affinity chromatography and confirmed by SDS-PAGE. It was shown that the recombinant ApoA Ⅰ was expressed in E.coli, and the target protein amounted to 36% of total bacteria proteins. Cholesteryl ester transfer experiment showed that the recombinant ApoA Ⅰ was capable of promoting transfer of CE from HDL to LDL. Western blotting showed that the protein could react specifically with anti-ApoA Ⅰ antibodies.
