Intelligent optimization algorithm belongs to a kind of emerging technology,show good characteristics,such as high performance,applicability,its algorithm includes many contents,including genetic,particle swarm and ar...Intelligent optimization algorithm belongs to a kind of emerging technology,show good characteristics,such as high performance,applicability,its algorithm includes many contents,including genetic,particle swarm and artificial neural network algorithm,compared with the traditional optimization way,these algorithms can be applied to a variety of situations,meet the demand of solution,in the mechanical design industry has wide application prospects.This paper analyzes the application of the algorithm in mechanical design and the comparison of the results to verify the significance of the intelligent optimization algorithm in mechanical design.展开更多
This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extens...This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extensively planted but not resistant to anthrax. The reciprocal crosses were performed using Benihoppe, as the female parent, four disease-resistant European cultivars Albion, Camino Real, Ven- tana and Virginia, and one Japanese cultivar Meiho as the male parent. Then, the seedling propagation coefficient, disease resistance, fruit quality and yield of F1 gen- eration of the combinations were detected and compared. Besides, the breeding and traits of a new cultivar F1-40 developed from the cross between Benihoppe and Mei- ho were introduced as an instance to prove the feasibility of the combinations/lines.展开更多
In this paper,we offer a review of type-3 fuzzy logic systems and their applications in control.The main objective of this work is to observe and analyze in detail the applications in the control area using type-3 fuz...In this paper,we offer a review of type-3 fuzzy logic systems and their applications in control.The main objective of this work is to observe and analyze in detail the applications in the control area using type-3 fuzzy logic systems.In this case,we review their most important applications in control and other related topics with type-3 fuzzy systems.Intelligent algorithms have been receiving increasing attention in control and for this reason a review in this area is important.This paper reviews the main applications that make use of Intelligent Computing methods.Specifically,type-3 fuzzy logic systems.The aim of this research is to be able to appreciate,in detail,the applications in control systems and to point out the scientific trends in the use of Intelligent Computing techniques.This is done with the construction and visualization of bibliometric networks,developed with VosViewer Software,which it is a free Java-based program,mainly intended to be used for analyzing and visualizing bibliometric networks.With this tool,we can create maps of publications,authors,or journals based on a co-citation network or construct maps of keywords,countries based on a co-occurrence networks,research groups,etc.展开更多
Demand-responsive transportation(DRT)is a flexible passenger service designed to enhance road efficiency,reduce peak-hour traffic,and boost passenger satisfaction.However,existing optimization methods for initial pass...Demand-responsive transportation(DRT)is a flexible passenger service designed to enhance road efficiency,reduce peak-hour traffic,and boost passenger satisfaction.However,existing optimization methods for initial passenger requests fall short in addressing real-time passenger needs.Consequently,there is a need to develop realtime DRT route optimization methods that integrate both initial and real-time requests.This paper presents a twostage,multi-objective optimization model for DRT vehicle scheduling.The first stage involves an initial scheduling model aimed at minimizing vehicle configuration,and operational,and CO_(2)emission costs while ensuring passenger satisfaction.The second stage develops a real-time scheduling model to minimize additional operational costs,penalties for time window violations,and costs due to rejected passengers,thereby addressing real-time demands.Additionally,an enhanced genetic algorithm based on Non-dominated Sorting Genetic Algorithm-II(NSGA-II)is designed,incorporating multiple crossover points to accelerate convergence and improve solution efficiency.The proposed scheduling model is validated using a real network in Shanghai.Results indicate that realtime scheduling can serve more passengers,and improve vehicle utilization and occupancy rates,with only a minor increase in total operational costs.Compared to the traditional NSGA-II algorithm,the improved version enhances convergence speed by 31.7%and solution speed by 4.8%.The proposed model and algorithm offer both theoretical and practical guidance for real-world DRT scheduling.展开更多
This paper presents a neighborhood optimal trajectory online correction algorithm considering terminal time variation,and investigates its application range.Firstly,the motion model of midcourse guidance is establishe...This paper presents a neighborhood optimal trajectory online correction algorithm considering terminal time variation,and investigates its application range.Firstly,the motion model of midcourse guidance is established,and the online trajectory correction-regenerating strategy is introduced.Secondly,based on the neighborhood optimal control theory,a neighborhood optimal trajectory online correction algorithm considering the terminal time variation is proposed by adding the consideration of terminal time variation to the traditional neighborhood optimal trajectory correction method.Thirdly,the Monte Carlo simulation method is used to analyze the application range of the algorithm,which provides a basis for the division of application domain of the online correction algorithm and the online regeneration algorithm of midcourse guidance trajectory.Finally,the simulation results show that the algorithm has high real-time performance,and the online correction trajectory can meet the requirements of terminal constraint change.The application range of the algorithm is obtained through Monte Carlo simulation.展开更多
In the smart city paradigm, the deployment of Internet of Things(IoT) services and solutions requires extensive communication and computingresources to place and process IoT applications in real time, which consumesa ...In the smart city paradigm, the deployment of Internet of Things(IoT) services and solutions requires extensive communication and computingresources to place and process IoT applications in real time, which consumesa lot of energy and increases operational costs. Usually, IoT applications areplaced in the cloud to provide high-quality services and scalable resources.However, the existing cloud-based approach should consider the above constraintsto efficiently place and process IoT applications. In this paper, anefficient optimization approach for placing IoT applications in a multi-layerfog-cloud environment is proposed using a mathematical model (Mixed-Integer Linear Programming (MILP)). This approach takes into accountIoT application requirements, available resource capacities, and geographicallocations of servers, which would help optimize IoT application placementdecisions, considering multiple objectives such as data transmission, powerconsumption, and cost. Simulation experiments were conducted with variousIoT applications (e.g., augmented reality, infotainment, healthcare, andcompute-intensive) to simulate realistic scenarios. The results showed thatthe proposed approach outperformed the existing cloud-based approach interms of reducing data transmission by 64% and the associated processingand networking power consumption costs by up to 78%. Finally, a heuristicapproach was developed to validate and imitate the presented approach. Itshowed comparable outcomes to the proposed model, with the gap betweenthem reach to a maximum of 5.4% of the total power consumption.展开更多
Aqueous supercapacitors(SCs)have been regarded as a promising candidate for commercial energy storage device due to their superior safety,low cost,and environmental benignity.Unfortunately,an age-old challenge of achi...Aqueous supercapacitors(SCs)have been regarded as a promising candidate for commercial energy storage device due to their superior safety,low cost,and environmental benignity.Unfortunately,an age-old challenge of achieving both long electrode lifespan and qualified energy-storage property blocks their practical application.Herein,we develop an electrode-electrolyte integrated optimization strategy to fulfill the real-life device requirements.Electrode optimization simultaneously regulates the nanomorphology and surface chemistry of the tungsten oxide anode,resulting in superior electrochemical performance given by an ideal“bird-nest”structure with optimal oxygen vacancy status;the anodes interact with and are protected from dissolution and structural collapse by the rationally designed hybrid electrolyte with optimized pH and facilitated cation desorption behavior.Collaboratively,a record-breaking durability of no capacitive decay after 250000 cycles is achieved.On the basis of this integrated optimization,the first aqueous pouch SCs with real-life practicability were manufactured by a soft-package encapsulation technique,which can steadily power commercial 3 C products such as tablets and smartphones and maintain safely working against extreme conditions.This work demonstrates the possibility of using aqueous energy storage devices with enhanced safety and lower cost to replace the commercial organic counterparts for wide range of daily applications.展开更多
For sludge samples from EBPR reactor fed with municipal wastewater,fluorescent in situ hybridization (FISH) operation process including moisture chamber,pretreatment,treatment with lysozyme and Proteinase K and washin...For sludge samples from EBPR reactor fed with municipal wastewater,fluorescent in situ hybridization (FISH) operation process including moisture chamber,pretreatment,treatment with lysozyme and Proteinase K and washing time was optimized and improved.Preserving box was chosen to be moisture chamber due to its bigger depth /radii ratio,good sealability and big volume contrast with Petri dish.3-5 mm diameter glass balls could disperse samples without destroying microorganism cells and community structure.Impurities and ECPs could be removed easily and sludge samples became thinner after dispersing which benefit the observation.Permeabilized cells with lysozyme and Proteinase K could enhance probe penetration before hybridization.Experiments of different treatment time with lysozyme and Proteinase K were carried out.Best results were observed when sludge samples treated with lysozyme 10 min/Proteinase K 20 min or lysozyme 20 min/Proteinase K 10 min.Slides were washed at 48 ℃ for 10,20,30,40 and 60 min in parallel.The best washing time was 20 min when washing temperature was 48 ℃.Fluorescent dye could residue when washing time was 10 min and washing out happened when washed for 30 min or more.展开更多
During the last few years we have witnessed impressive developments in the area of stochastic local search techniques for intelligent optimization and Reactive Search Optimization. In order to handle the complexity, i...During the last few years we have witnessed impressive developments in the area of stochastic local search techniques for intelligent optimization and Reactive Search Optimization. In order to handle the complexity, in the framework of stochastic local search optimization, learning and optimization has been deeply interconnected through interaction with the decision maker via the visualization approach of the online graphs. Consequently a number of complex optimization problems, in particular multiobjective optimization problems, arising in widely different contexts have been effectively treated within the general framework of RSO. In solving real-life multiobjective optimization problems often most emphasis are spent on finding the complete Pareto-optimal set and less on decision-making. However the com-plete task of multiobjective optimization is considered as a combined task of optimization and decision-making. In this paper, we suggest an interactive procedure which will involve the decision-maker in the optimization process helping to choose a single solution at the end. Our proposed method works on the basis of Reactive Search Optimization (RSO) algorithms and available software architecture packages. The procedure is further compared with the excising novel method of Interactive Multiobjective Optimization and Decision-Making, using Evolutionary method (I-MODE). In order to evaluate the effectiveness of both methods the well-known study case of welded beam design problem is reconsidered.展开更多
In this paper, based on existing results, decision making about portfolio investment schemes is discussed, ordering method of fuzzy numbers of interval value is shown, corresponding auxiliary models are established an...In this paper, based on existing results, decision making about portfolio investment schemes is discussed, ordering method of fuzzy numbers of interval value is shown, corresponding auxiliary models are established and solutions are provided with theories of fuzzy mathematics, optimization theory and numerical calculation, etc. Then it applies software programming to solve the portfolio investment situation between investors in savings and four securities according to the established models. The result shows that investors can choose the risk coefficient that they can bear to reach the maximum value of expected returns. The greater the risk coefficient, the greater the income, the smaller the risk coefficient and the smaller the income. Investors can determine their own portfolio strategy according to their own conditions in order to meet their own interests.展开更多
In this study, three methods such as CTAB,SDS and Shanlichun methods were used to extract genomic DNA from the seedling of rape to find the best method. The principle, characters and application of SRAP were introduce...In this study, three methods such as CTAB,SDS and Shanlichun methods were used to extract genomic DNA from the seedling of rape to find the best method. The principle, characters and application of SRAP were introduced. In order to obtain the optimal SRAP reaction system, the factors including concentrations of DNA, dNTP, etc. of reaction system were modified to better the system of rape. Th9 result showed that the optimum concentrations were 15ng DNA template, 0.2mM dNTP, 1.0μM primer and 2.0U Taq enzyme in this 25μL SRAP-PCR system.展开更多
Thin films of cadmium sulphide and cadmium telluride have been prepared by thermal evaporation under various conditions of deposition. These films have been characterized optically. electrically and for structure dete...Thin films of cadmium sulphide and cadmium telluride have been prepared by thermal evaporation under various conditions of deposition. These films have been characterized optically. electrically and for structure determination. The results of these characterizations along with the initial results of all thin film CdS/CdTe solar cells are presented in this paper展开更多
In this paper, we conduct research on the golden thoughts and topology optimization and the applications on the environment space design. Environmental art and a science and art, is to create lasting and harmonious an...In this paper, we conduct research on the golden thoughts and topology optimization and the applications on the environment space design. Environmental art and a science and art, is to create lasting and harmonious and the urban design, architectural design, murals, such as urban planning belong to the scope of environmental art and the people’s life, work, production and leisure are closely linked, along with people living standard and the improvement of living level, people have higher request for all kinds of the environmental quality. Our research combines the general principles of the related theories to propose the novel perspective that is innovative.展开更多
The current status of university computer teaching, taking fully into account the differences between students, competency-based education philosophy as a guide, this paper discusses the application of modular and hie...The current status of university computer teaching, taking fully into account the differences between students, competency-based education philosophy as a guide, this paper discusses the application of modular and hierarchical optimization problems in teaching computer courses in colleges and universities, and in-depth analyzes the basic content and implementation strategies of module level teaching.展开更多
This paper introduces a video application-aware cross-layer framework for joint performance-energy optimization,considering the scenario of multiple users upstreaming real-time Motion JPEG2000 video streams to the acc...This paper introduces a video application-aware cross-layer framework for joint performance-energy optimization,considering the scenario of multiple users upstreaming real-time Motion JPEG2000 video streams to the access point of a WiFi wireless local area network and extends the PHY-MAC run-time cross-layer scheduling strategy that we introduced in (Mangharam et al., 2005; Pollin et al., 2005) to also consider congested network situations where video packets have to be dropped. We show that an optimal solution at PHY-MAC level can be highly suboptimal at application level, and then show that making the cross-layer framework application-aware through a prioritized dropping policy capitalizing on the inherent scalability of Motion JPEG2000 video streams leads to drastic average video quality improvements and inter-user quality variation reductions of as much as 10 dB PSNR, without affecting the overall energy consumption requirements.展开更多
Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the an...Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged.展开更多
The growth of Wireless Sensor Networks(WSNs)has revolutionized thefield of technology and it is used in different application frameworks.Unmanned edges and other critical locations can be monitored using the navigatio...The growth of Wireless Sensor Networks(WSNs)has revolutionized thefield of technology and it is used in different application frameworks.Unmanned edges and other critical locations can be monitored using the navigation sensor node.The WSN required low energy consumption to provide a high network and guarantee the ultimate goal.The main objective of this work is to propose hybrid energy optimization in local aware environments.The hybrid proposed work consists of clustering,optimization,direct and indirect communication and routing.The aim of this research work is to provide and framework for reduced energy and trusted communication with the shortest path to reach source to destination in WSN and an extending lifetime of wireless sensors.The proposed Artificial Fish Swarm Optimization algorithm is used for energy optimization in military applications which is simulated using Network Simulator(NS)tool.This work optimizes the energy level and the same is compared with various genetic algorithms(GA)and also the cluster selection process was compared with thefission-fusion(FF)selection method.The results of the proposed work show,improvement in energy optimization,throughput and time delay.展开更多
文摘Intelligent optimization algorithm belongs to a kind of emerging technology,show good characteristics,such as high performance,applicability,its algorithm includes many contents,including genetic,particle swarm and artificial neural network algorithm,compared with the traditional optimization way,these algorithms can be applied to a variety of situations,meet the demand of solution,in the mechanical design industry has wide application prospects.This paper analyzes the application of the algorithm in mechanical design and the comparison of the results to verify the significance of the intelligent optimization algorithm in mechanical design.
基金Supported by Fund for Independent Innovation in Agricultural Science and Technology of Jiangsu Province[CX(13)2012]~~
文摘This study was conducted to select new strawberry cultivars with early maturity, disease resistance and high quality, by optimizing the hybrid combinations of Benihoppe, a Japanese strawberry cultivar, which is extensively planted but not resistant to anthrax. The reciprocal crosses were performed using Benihoppe, as the female parent, four disease-resistant European cultivars Albion, Camino Real, Ven- tana and Virginia, and one Japanese cultivar Meiho as the male parent. Then, the seedling propagation coefficient, disease resistance, fruit quality and yield of F1 gen- eration of the combinations were detected and compared. Besides, the breeding and traits of a new cultivar F1-40 developed from the cross between Benihoppe and Mei- ho were introduced as an instance to prove the feasibility of the combinations/lines.
基金CONAHCYTTecnológico Nacional de Mexico/Tijuana Institute of Technology for the support during this research
文摘In this paper,we offer a review of type-3 fuzzy logic systems and their applications in control.The main objective of this work is to observe and analyze in detail the applications in the control area using type-3 fuzzy logic systems.In this case,we review their most important applications in control and other related topics with type-3 fuzzy systems.Intelligent algorithms have been receiving increasing attention in control and for this reason a review in this area is important.This paper reviews the main applications that make use of Intelligent Computing methods.Specifically,type-3 fuzzy logic systems.The aim of this research is to be able to appreciate,in detail,the applications in control systems and to point out the scientific trends in the use of Intelligent Computing techniques.This is done with the construction and visualization of bibliometric networks,developed with VosViewer Software,which it is a free Java-based program,mainly intended to be used for analyzing and visualizing bibliometric networks.With this tool,we can create maps of publications,authors,or journals based on a co-citation network or construct maps of keywords,countries based on a co-occurrence networks,research groups,etc.
文摘Demand-responsive transportation(DRT)is a flexible passenger service designed to enhance road efficiency,reduce peak-hour traffic,and boost passenger satisfaction.However,existing optimization methods for initial passenger requests fall short in addressing real-time passenger needs.Consequently,there is a need to develop realtime DRT route optimization methods that integrate both initial and real-time requests.This paper presents a twostage,multi-objective optimization model for DRT vehicle scheduling.The first stage involves an initial scheduling model aimed at minimizing vehicle configuration,and operational,and CO_(2)emission costs while ensuring passenger satisfaction.The second stage develops a real-time scheduling model to minimize additional operational costs,penalties for time window violations,and costs due to rejected passengers,thereby addressing real-time demands.Additionally,an enhanced genetic algorithm based on Non-dominated Sorting Genetic Algorithm-II(NSGA-II)is designed,incorporating multiple crossover points to accelerate convergence and improve solution efficiency.The proposed scheduling model is validated using a real network in Shanghai.Results indicate that realtime scheduling can serve more passengers,and improve vehicle utilization and occupancy rates,with only a minor increase in total operational costs.Compared to the traditional NSGA-II algorithm,the improved version enhances convergence speed by 31.7%and solution speed by 4.8%.The proposed model and algorithm offer both theoretical and practical guidance for real-world DRT scheduling.
基金supported by the National Natural Science Foundation of China(61873278,62173339)。
文摘This paper presents a neighborhood optimal trajectory online correction algorithm considering terminal time variation,and investigates its application range.Firstly,the motion model of midcourse guidance is established,and the online trajectory correction-regenerating strategy is introduced.Secondly,based on the neighborhood optimal control theory,a neighborhood optimal trajectory online correction algorithm considering the terminal time variation is proposed by adding the consideration of terminal time variation to the traditional neighborhood optimal trajectory correction method.Thirdly,the Monte Carlo simulation method is used to analyze the application range of the algorithm,which provides a basis for the division of application domain of the online correction algorithm and the online regeneration algorithm of midcourse guidance trajectory.Finally,the simulation results show that the algorithm has high real-time performance,and the online correction trajectory can meet the requirements of terminal constraint change.The application range of the algorithm is obtained through Monte Carlo simulation.
文摘In the smart city paradigm, the deployment of Internet of Things(IoT) services and solutions requires extensive communication and computingresources to place and process IoT applications in real time, which consumesa lot of energy and increases operational costs. Usually, IoT applications areplaced in the cloud to provide high-quality services and scalable resources.However, the existing cloud-based approach should consider the above constraintsto efficiently place and process IoT applications. In this paper, anefficient optimization approach for placing IoT applications in a multi-layerfog-cloud environment is proposed using a mathematical model (Mixed-Integer Linear Programming (MILP)). This approach takes into accountIoT application requirements, available resource capacities, and geographicallocations of servers, which would help optimize IoT application placementdecisions, considering multiple objectives such as data transmission, powerconsumption, and cost. Simulation experiments were conducted with variousIoT applications (e.g., augmented reality, infotainment, healthcare, andcompute-intensive) to simulate realistic scenarios. The results showed thatthe proposed approach outperformed the existing cloud-based approach interms of reducing data transmission by 64% and the associated processingand networking power consumption costs by up to 78%. Finally, a heuristicapproach was developed to validate and imitate the presented approach. Itshowed comparable outcomes to the proposed model, with the gap betweenthem reach to a maximum of 5.4% of the total power consumption.
基金supported by the National Natural Science Foundation of China(Nos.52071171,52202248 and 22209064)Liaoning Revitalization Talents Program—Pan Deng Scholars(XLYC1802005)+9 种基金Liaoning Bai Qian Wan Talents Program(LNBQW2018B0048)Key Project of Scientific Research of the Education Department of Liaoning Province(LZD201902)Shenyang Science and Technology Project(21-108-9-04)Australian Research Council(ARC)through Future Fel owship(FT210100298,FT210100806)Discovery Project(DP220100603)Linkage Project(LP210100467,LP210200504,and LP210200345)Industrial Transformation Training Centre(IC180100005)schemes,CSIRO Energy Centre and Kick-Start ProjectStudy Melbourne Research Partnerships program has been made possible by funding from the Victorian Government through Study MelbourneShiyanjia Lab(www.shiyanjia.com)for the support of the XPS testsupport from the University of Calgary’s Canada First Research Excel ence Fund program,the Global Research Initiative for Sustainable Low-Carbon Unconventional Energy
文摘Aqueous supercapacitors(SCs)have been regarded as a promising candidate for commercial energy storage device due to their superior safety,low cost,and environmental benignity.Unfortunately,an age-old challenge of achieving both long electrode lifespan and qualified energy-storage property blocks their practical application.Herein,we develop an electrode-electrolyte integrated optimization strategy to fulfill the real-life device requirements.Electrode optimization simultaneously regulates the nanomorphology and surface chemistry of the tungsten oxide anode,resulting in superior electrochemical performance given by an ideal“bird-nest”structure with optimal oxygen vacancy status;the anodes interact with and are protected from dissolution and structural collapse by the rationally designed hybrid electrolyte with optimized pH and facilitated cation desorption behavior.Collaboratively,a record-breaking durability of no capacitive decay after 250000 cycles is achieved.On the basis of this integrated optimization,the first aqueous pouch SCs with real-life practicability were manufactured by a soft-package encapsulation technique,which can steadily power commercial 3 C products such as tablets and smartphones and maintain safely working against extreme conditions.This work demonstrates the possibility of using aqueous energy storage devices with enhanced safety and lower cost to replace the commercial organic counterparts for wide range of daily applications.
基金Sponsored by the National Natural Science Foundation of China (Grant No. 50638020)
文摘For sludge samples from EBPR reactor fed with municipal wastewater,fluorescent in situ hybridization (FISH) operation process including moisture chamber,pretreatment,treatment with lysozyme and Proteinase K and washing time was optimized and improved.Preserving box was chosen to be moisture chamber due to its bigger depth /radii ratio,good sealability and big volume contrast with Petri dish.3-5 mm diameter glass balls could disperse samples without destroying microorganism cells and community structure.Impurities and ECPs could be removed easily and sludge samples became thinner after dispersing which benefit the observation.Permeabilized cells with lysozyme and Proteinase K could enhance probe penetration before hybridization.Experiments of different treatment time with lysozyme and Proteinase K were carried out.Best results were observed when sludge samples treated with lysozyme 10 min/Proteinase K 20 min or lysozyme 20 min/Proteinase K 10 min.Slides were washed at 48 ℃ for 10,20,30,40 and 60 min in parallel.The best washing time was 20 min when washing temperature was 48 ℃.Fluorescent dye could residue when washing time was 10 min and washing out happened when washed for 30 min or more.
文摘During the last few years we have witnessed impressive developments in the area of stochastic local search techniques for intelligent optimization and Reactive Search Optimization. In order to handle the complexity, in the framework of stochastic local search optimization, learning and optimization has been deeply interconnected through interaction with the decision maker via the visualization approach of the online graphs. Consequently a number of complex optimization problems, in particular multiobjective optimization problems, arising in widely different contexts have been effectively treated within the general framework of RSO. In solving real-life multiobjective optimization problems often most emphasis are spent on finding the complete Pareto-optimal set and less on decision-making. However the com-plete task of multiobjective optimization is considered as a combined task of optimization and decision-making. In this paper, we suggest an interactive procedure which will involve the decision-maker in the optimization process helping to choose a single solution at the end. Our proposed method works on the basis of Reactive Search Optimization (RSO) algorithms and available software architecture packages. The procedure is further compared with the excising novel method of Interactive Multiobjective Optimization and Decision-Making, using Evolutionary method (I-MODE). In order to evaluate the effectiveness of both methods the well-known study case of welded beam design problem is reconsidered.
文摘In this paper, based on existing results, decision making about portfolio investment schemes is discussed, ordering method of fuzzy numbers of interval value is shown, corresponding auxiliary models are established and solutions are provided with theories of fuzzy mathematics, optimization theory and numerical calculation, etc. Then it applies software programming to solve the portfolio investment situation between investors in savings and four securities according to the established models. The result shows that investors can choose the risk coefficient that they can bear to reach the maximum value of expected returns. The greater the risk coefficient, the greater the income, the smaller the risk coefficient and the smaller the income. Investors can determine their own portfolio strategy according to their own conditions in order to meet their own interests.
文摘In this study, three methods such as CTAB,SDS and Shanlichun methods were used to extract genomic DNA from the seedling of rape to find the best method. The principle, characters and application of SRAP were introduced. In order to obtain the optimal SRAP reaction system, the factors including concentrations of DNA, dNTP, etc. of reaction system were modified to better the system of rape. Th9 result showed that the optimum concentrations were 15ng DNA template, 0.2mM dNTP, 1.0μM primer and 2.0U Taq enzyme in this 25μL SRAP-PCR system.
文摘Thin films of cadmium sulphide and cadmium telluride have been prepared by thermal evaporation under various conditions of deposition. These films have been characterized optically. electrically and for structure determination. The results of these characterizations along with the initial results of all thin film CdS/CdTe solar cells are presented in this paper
文摘In this paper, we conduct research on the golden thoughts and topology optimization and the applications on the environment space design. Environmental art and a science and art, is to create lasting and harmonious and the urban design, architectural design, murals, such as urban planning belong to the scope of environmental art and the people’s life, work, production and leisure are closely linked, along with people living standard and the improvement of living level, people have higher request for all kinds of the environmental quality. Our research combines the general principles of the related theories to propose the novel perspective that is innovative.
文摘The current status of university computer teaching, taking fully into account the differences between students, competency-based education philosophy as a guide, this paper discusses the application of modular and hierarchical optimization problems in teaching computer courses in colleges and universities, and in-depth analyzes the basic content and implementation strategies of module level teaching.
文摘This paper introduces a video application-aware cross-layer framework for joint performance-energy optimization,considering the scenario of multiple users upstreaming real-time Motion JPEG2000 video streams to the access point of a WiFi wireless local area network and extends the PHY-MAC run-time cross-layer scheduling strategy that we introduced in (Mangharam et al., 2005; Pollin et al., 2005) to also consider congested network situations where video packets have to be dropped. We show that an optimal solution at PHY-MAC level can be highly suboptimal at application level, and then show that making the cross-layer framework application-aware through a prioritized dropping policy capitalizing on the inherent scalability of Motion JPEG2000 video streams leads to drastic average video quality improvements and inter-user quality variation reductions of as much as 10 dB PSNR, without affecting the overall energy consumption requirements.
基金This work was supported by the Major Science and Technology Projects in Anhui Province,China(202003b06020021)the Natural Science Foundation of Anhui Province,China(2008085QC122)+1 种基金the Postgraduate Quality Engineering Project in Anhui Province,China(2022cxcysj0066)the Special Fund for Anhui Agriculture Research System,China.
文摘Extreme low-temperature incidents have become more frequent and severe as climate change intensifies.In HuangHuai-Hai wheat growing area of China,the late spring coldness occurring at the jointing-booting stage(the anther interval stage)has resulted in significant yield losses of winter wheat.This study attempts to develop an economical,feasible,and efficient cultivation technique for improving the low-temperature(LT)resistance of wheat by exploring the effects of twice-split phosphorus application(TSPA)on wheat antioxidant characteristics and carbon and nitrogen metabolism physiology under LT treatment at the anther interval stage using Yannong 19 as the experimental material.The treatments consisted of traditional phosphorus application and TSPA,followed by a-4℃ LT treatment and natural temperature(NT)control at the anther interval stage.Our analyses showed that,compared with the traditional application,the TSPA increased the net photosynthetic rate(P_(n)),stomatal conductance(Gs),and transpiration rate(T_(r))of leaves and reduced the intercellular carbon dioxide concentration(C_(i)).The activity of carbon and nitrogen metabolism enzymes in the young wheat spikes was also increased by the TSPA,which promoted the accumulation of soluble sugar(SS),sucrose(SUC),soluble protein(SP),and proline(Pro)in young wheat spike and reduced the toxicity of malondialdehyde(MDA).Due to the improved organic nutrition for reproductive development,the young wheat spikes exhibited enhanced LT resistance,which reduced the sterile spikelet number(SSN)per spike by 11.8%and increased the spikelet setting rate(SSR)and final yield by 6.0 and 8.4%,respectively,compared to the traditional application.The positive effects of split phosphorus application became more pronounced when the LT treatment was prolonged.
文摘The growth of Wireless Sensor Networks(WSNs)has revolutionized thefield of technology and it is used in different application frameworks.Unmanned edges and other critical locations can be monitored using the navigation sensor node.The WSN required low energy consumption to provide a high network and guarantee the ultimate goal.The main objective of this work is to propose hybrid energy optimization in local aware environments.The hybrid proposed work consists of clustering,optimization,direct and indirect communication and routing.The aim of this research work is to provide and framework for reduced energy and trusted communication with the shortest path to reach source to destination in WSN and an extending lifetime of wireless sensors.The proposed Artificial Fish Swarm Optimization algorithm is used for energy optimization in military applications which is simulated using Network Simulator(NS)tool.This work optimizes the energy level and the same is compared with various genetic algorithms(GA)and also the cluster selection process was compared with thefission-fusion(FF)selection method.The results of the proposed work show,improvement in energy optimization,throughput and time delay.