AIM:To determine hepatic expression of apurinic/apyrimidinic endonuclease 1(APE-1)and 8-hydroxydeoxyguanosine(8-OHdG)in patients with chronic hepatitis B and C.METHODS:Liver biopsies were obtained from 27 patients wit...AIM:To determine hepatic expression of apurinic/apyrimidinic endonuclease 1(APE-1)and 8-hydroxydeoxyguanosine(8-OHdG)in patients with chronic hepatitis B and C.METHODS:Liver biopsies were obtained from 27 patients with chronic hepatitis B virus(HBV),30 with chronic hepatitis C virus(HCV),6 with autoimmune hepatitis(AIH),and 6 with primary biliary cirrhosis(PBC).Normal liver tissue was obtained from surgical resection specimens of four patients.Hepatic APE-1 protein and mRNA expression were assayed by Western blot and by real-time polymerase chain reaction,respectively.Hepatocellular APE-1 and 8-OHdG expression were determined by immunohistochemistry.RESULTS:The staining intensity of hepatocellular nuclear APE-1 was lower in the HBV group than in the other groups(P < 0.05).Hepatic APE-1 protein levels were reduced in the HBV group relative to the other groups.Hepatic APE-1 mRNA levels were also lower in the HBV group.The proportion of hepatocytes with 8-OHdG-positive nuclei was increased in the HCV,AIH and PBC groups(P < 0.05),but not in the HBV group.Hepatocellular nuclear APE-1 levels were positively correlated with hepatocellular 8-OHdG levels in both the HBV and HCV groups(HBV,r = 0.34,P < 0.05;HCV,r = 0.54,P < 0.01).CONCLUSION:An imbalance between oxidative DNA damage and APE-1 expression may contribute to hepatocarcinogenesis in chronic viral hepatitis.展开更多
Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage cause...Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apudnic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.展开更多
目的探讨早期宫颈鳞癌组织中程序性死亡配体-1(programmed death ligand-1,PD-L1)和脱嘌呤/脱嘧啶内切核酸酶1(apurinic/apyrimidinic endonuclease1,APE1)的表达与临床特征及预后的关系。方法采用免疫组织化学的方法(IHC)检测PD-L1和A...目的探讨早期宫颈鳞癌组织中程序性死亡配体-1(programmed death ligand-1,PD-L1)和脱嘌呤/脱嘧啶内切核酸酶1(apurinic/apyrimidinic endonuclease1,APE1)的表达与临床特征及预后的关系。方法采用免疫组织化学的方法(IHC)检测PD-L1和APE1蛋白在64例早期宫颈鳞癌组织中的表达,分析PD-L1、APE1表达以及两者共表达与早期宫颈癌临床特征的关系;采用Spearman等级相关分析检测APE1与PD-L1表达的相关性,Kaplan-Meier法分析早期宫颈鳞癌患者中APE1与PD-L1的表达与术后生存的关系,Cox回归模型分析早期宫颈鳞癌患者预后的危险因素。结果早期宫颈癌患者APE1和PD-L1蛋白表达阳性率分别为70.31%和67.18%。PD-L1的表达与分期、淋巴结转移有显著相关性(P<0.05),而APE1的表达仅与淋巴结转移密切相关(P=0.038)。另外,APE1与PD-L1的表达正相关(r2=0.347,P=0.005),且两者共表达与淋巴结转移相关,其术后生存期比单一阳性表达的宫颈癌患者明显缩短(P<0.05)。多因素COX回归模型结果显示,APE1的表达和淋巴结转移是影响早期宫颈鳞癌预后的独立危险因素,危险度分别为16.187(P=0.01)和27.340(P<0.001)。结论PD-L1和APE1表达的联合检测对早期宫颈鳞癌预后评估具有潜在的临床价值。展开更多
文摘AIM:To determine hepatic expression of apurinic/apyrimidinic endonuclease 1(APE-1)and 8-hydroxydeoxyguanosine(8-OHdG)in patients with chronic hepatitis B and C.METHODS:Liver biopsies were obtained from 27 patients with chronic hepatitis B virus(HBV),30 with chronic hepatitis C virus(HCV),6 with autoimmune hepatitis(AIH),and 6 with primary biliary cirrhosis(PBC).Normal liver tissue was obtained from surgical resection specimens of four patients.Hepatic APE-1 protein and mRNA expression were assayed by Western blot and by real-time polymerase chain reaction,respectively.Hepatocellular APE-1 and 8-OHdG expression were determined by immunohistochemistry.RESULTS:The staining intensity of hepatocellular nuclear APE-1 was lower in the HBV group than in the other groups(P < 0.05).Hepatic APE-1 protein levels were reduced in the HBV group relative to the other groups.Hepatic APE-1 mRNA levels were also lower in the HBV group.The proportion of hepatocytes with 8-OHdG-positive nuclei was increased in the HCV,AIH and PBC groups(P < 0.05),but not in the HBV group.Hepatocellular nuclear APE-1 levels were positively correlated with hepatocellular 8-OHdG levels in both the HBV and HCV groups(HBV,r = 0.34,P < 0.05;HCV,r = 0.54,P < 0.01).CONCLUSION:An imbalance between oxidative DNA damage and APE-1 expression may contribute to hepatocarcinogenesis in chronic viral hepatitis.
基金supported by the National Natural Science Foundation of China, No. 30571790
文摘Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apudnic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.
文摘目的探讨早期宫颈鳞癌组织中程序性死亡配体-1(programmed death ligand-1,PD-L1)和脱嘌呤/脱嘧啶内切核酸酶1(apurinic/apyrimidinic endonuclease1,APE1)的表达与临床特征及预后的关系。方法采用免疫组织化学的方法(IHC)检测PD-L1和APE1蛋白在64例早期宫颈鳞癌组织中的表达,分析PD-L1、APE1表达以及两者共表达与早期宫颈癌临床特征的关系;采用Spearman等级相关分析检测APE1与PD-L1表达的相关性,Kaplan-Meier法分析早期宫颈鳞癌患者中APE1与PD-L1的表达与术后生存的关系,Cox回归模型分析早期宫颈鳞癌患者预后的危险因素。结果早期宫颈癌患者APE1和PD-L1蛋白表达阳性率分别为70.31%和67.18%。PD-L1的表达与分期、淋巴结转移有显著相关性(P<0.05),而APE1的表达仅与淋巴结转移密切相关(P=0.038)。另外,APE1与PD-L1的表达正相关(r2=0.347,P=0.005),且两者共表达与淋巴结转移相关,其术后生存期比单一阳性表达的宫颈癌患者明显缩短(P<0.05)。多因素COX回归模型结果显示,APE1的表达和淋巴结转移是影响早期宫颈鳞癌预后的独立危险因素,危险度分别为16.187(P=0.01)和27.340(P<0.001)。结论PD-L1和APE1表达的联合检测对早期宫颈鳞癌预后评估具有潜在的临床价值。
