Objective: To elucidate the pattern of chemo therapy drugs induced apoptosis and its role in chemo therapy of acute leukemia. Methods: Apoptosis induced by Ara C in human myeloid leukemia cell line HL 60 was inve...Objective: To elucidate the pattern of chemo therapy drugs induced apoptosis and its role in chemo therapy of acute leukemia. Methods: Apoptosis induced by Ara C in human myeloid leukemia cell line HL 60 was investigated by applying light microscope, electron microscopy combined with DNA electrophoresis and flow cytometry analysis techniques. Results: Apoptosis per sisted throughout 36 h following addition of Ara C with a gradual augmentation. Efficiency of apoptosis was enhanced in a dosedependent pattern, HL 60 treated with six other chemotherapy drugs and peripheral white blood cells from a AML case undergoing DA regimen chemo therapy exhibited typical DNA ladder pattern. Further investigation indicated that chemotherapy drugs apop tosis came into being possibly by downregulating the expression of c myc and bcl 2 oncogenes. Conclusion: Chemotherapy induced apoptosis is the primary mecha nism of chemotherapy.展开更多
The myeloid leukemic cell line HL-60 was studied by using DNA gel electrophoresis, flow cytomery, McAb C-myc, McAb Bc1-2 and CFU-L. From zero to 36 h,the apoptosis rates of 8 different phases and other indexes were ob...The myeloid leukemic cell line HL-60 was studied by using DNA gel electrophoresis, flow cytomery, McAb C-myc, McAb Bc1-2 and CFU-L. From zero to 36 h,the apoptosis rates of 8 different phases and other indexes were observed. The results showed that with the prolonged time of drug incubation,apoptosis of HL-60 cells increased progressively. This effect can be enhanced obviously by rh-IL-3 and rh-GM-CSF. At the same time,the killed rate of leukemic cells by Ara-C induction was increased. C-myc expression was decreased and Bc1-2 expression did not display apparent change. Interestingly, the normal hemopoietic cells were not affected by these two kinds of cytokine. The theoretical basis was provided for concurrent use of rh-IL-3, rh-GM-CSF and cytotoxic drugs whose purpose is to elevate remission rate during the phase of induced remission of leukemia.展开更多
文摘Objective: To elucidate the pattern of chemo therapy drugs induced apoptosis and its role in chemo therapy of acute leukemia. Methods: Apoptosis induced by Ara C in human myeloid leukemia cell line HL 60 was investigated by applying light microscope, electron microscopy combined with DNA electrophoresis and flow cytometry analysis techniques. Results: Apoptosis per sisted throughout 36 h following addition of Ara C with a gradual augmentation. Efficiency of apoptosis was enhanced in a dosedependent pattern, HL 60 treated with six other chemotherapy drugs and peripheral white blood cells from a AML case undergoing DA regimen chemo therapy exhibited typical DNA ladder pattern. Further investigation indicated that chemotherapy drugs apop tosis came into being possibly by downregulating the expression of c myc and bcl 2 oncogenes. Conclusion: Chemotherapy induced apoptosis is the primary mecha nism of chemotherapy.
文摘The myeloid leukemic cell line HL-60 was studied by using DNA gel electrophoresis, flow cytomery, McAb C-myc, McAb Bc1-2 and CFU-L. From zero to 36 h,the apoptosis rates of 8 different phases and other indexes were observed. The results showed that with the prolonged time of drug incubation,apoptosis of HL-60 cells increased progressively. This effect can be enhanced obviously by rh-IL-3 and rh-GM-CSF. At the same time,the killed rate of leukemic cells by Ara-C induction was increased. C-myc expression was decreased and Bc1-2 expression did not display apparent change. Interestingly, the normal hemopoietic cells were not affected by these two kinds of cytokine. The theoretical basis was provided for concurrent use of rh-IL-3, rh-GM-CSF and cytotoxic drugs whose purpose is to elevate remission rate during the phase of induced remission of leukemia.
