[Objectives]This study was conducted to investigate the pharmacokinetic characteristics of arctigenin from water extract of Fructus Arctii in piglets,understand its absorption,distribution,transformation and excretion...[Objectives]This study was conducted to investigate the pharmacokinetic characteristics of arctigenin from water extract of Fructus Arctii in piglets,understand its absorption,distribution,transformation and excretion in piglets,and provide theoretical reference for the development and clinical use of new veterinary drugs.[Methods]Six healthy piglets,weighing(30.0±5.0)kg,were selected and administered by gavage with a Fructus Arctii water extract at 1.0 g/kg·bw.Blood was collected from the anterior vena cava at different time points.The concentration of arctigenin in pig plasma was determined by HPLC.The main pharmacokinetic parameters were:absorption half-life(t1/2 ka)(0.098±0.006)h,distribution half-life(t1/2α)(0.208±0.009)h,elimination half-life(t1/2β)(23.816±2.151)h,apparent volume of distribution(Vd)(32.212±4.033)L/kg,apparent volume of distribution(Vd)(32.212±4.033)L/kg,clearance(CLb)(2.384±1.589)L/(h·kg),peak time(tmax)(0.251±0.011)h,peak concentration(Cmax)(0.560±0.063)μg/ml,and the area under the curve(AUC)at the time of drug administration(9.620±0.752)μg·h/ml.[Results]After oral administration of the water extract powder of Fructus Arctii,arctigenin showed a two-compartment model with absorption in piglets,with the characteristics of fast absorption,wide distribution and slow elimination.Arctigenin might have a hepatoenteral circulation in piglets,and the drug effect could last for a long time.[Conclusions]This study provides a theoretical basis and reference for the development and clinical use of new veterinary drugs.展开更多
目的:利用知识图谱可视化展示牛蒡子在中医药领域的研究成果与热点,分析其研究现状及预测研究趋势,为牛蒡子的后续研究提供参考。方法:采用Citespace软件与VOSviewer软件对中国知网(CNKI)和Web of Science(WOS)数据库2001—2021年收录...目的:利用知识图谱可视化展示牛蒡子在中医药领域的研究成果与热点,分析其研究现状及预测研究趋势,为牛蒡子的后续研究提供参考。方法:采用Citespace软件与VOSviewer软件对中国知网(CNKI)和Web of Science(WOS)数据库2001—2021年收录的与牛蒡子研究相关的中文文献537篇、英文文献149篇,分别从发文量、作者、机构、关键词等方面进行计量学分析,绘制网络可视化图谱。结果:中国是对牛蒡子研究最多的国家,中、英文文献中发文量最多的机构均为辽宁中医药大学,载文量最多的中、英文期刊分别为《时珍国医国药》《Phytotherapy Research》。中、英文文献关键词分析结果显示研究方向主要集中于牛蒡子化学成分、药理作用、栽培种植、加工炮制。结论:目前研究热点主要是木脂素类化合物(牛蒡苷及牛蒡苷元)对炎症、肿瘤、糖尿病及其并发症等疾病的预防和治疗,对其抗炎、抗肿瘤、治疗糖尿病等的信号通路、基因靶点及机制的深入研究将是未来牛蒡子研究的主要发展趋势。展开更多
The present study was designed to optimize the processing of Fructus Arctii by response surface methodology(RSM).Based on single factor studies,a three-variable,three-level Box-Behnken design(BBD) was used to monitor ...The present study was designed to optimize the processing of Fructus Arctii by response surface methodology(RSM).Based on single factor studies,a three-variable,three-level Box-Behnken design(BBD) was used to monitor the effects of independent variables,including processing temperature and time,on the dependent variables.Response surfaces and contour plots of the contents of total lignans,chlorogenic acid,arctiin,and arctigenin were obtained through ultraviolet and visible(UV-Vis) monitoring and high performance liquid chromatography(HPLC).Fructus Arctii should be processed under heating in a pot at 311℃,medicine at 119 °C for 123 s with flipping frequently.The experimental values under the optimized processing technology were consistent with the predicted values.In conclusion,RSM is an effective method to optimize the processing of traditional Chinese medicine(TCM).展开更多
[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control soluti...[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control solution, chlorogenic acid control solution and Tangjiangshenkang granule test solution in the range of 200-400 nm. In the ultraviolet scanning diagram of arctiin reference solution, the maximum absorption wavelength of 280 nm was determined as the determination wavelength λ 1, the detection wavelength in the ultraviolet scanning diagram of chlorogenic acid reference solution ( λ 1=280 nm) was determined, and 350 nm was the reference wavelength λ 2;the content of total lignosides in Tangjiangshenkang granules was determined with arctiin as the reference substance. [Results] The precision, accuracy, and durability of this method were fine. The concentration of arctiin was linearly correlated with the absorbance difference in the range of 0.007 95-0.071 55 mg/mL ( r =0. 999 9). The average recovery of arctiin was 100.8%, and the RSD value was 1.04% ( n =6). Calculated as arctiin, three batches of Tangjiangshenkang granules contain no less than 20% of total lignosides. [Conclusions] The method has the advantages of simple operation, good accuracy, precision and reliable stability. It can be used as the content determination and quality control method of total lignosides in Tangjiangshenkang granules.展开更多
基金Hubei Provincial Technical Innovation Major Project(2019AEE006)Wuhan Animal and Poultry Chinese Herbal Medicine Development Engineering Technology Research Center(2014021511020460)Hubei Provincial Central Government Guiding Local Science and Technology Development Fundation of China(2020ZYYD029)。
文摘[Objectives]This study was conducted to investigate the pharmacokinetic characteristics of arctigenin from water extract of Fructus Arctii in piglets,understand its absorption,distribution,transformation and excretion in piglets,and provide theoretical reference for the development and clinical use of new veterinary drugs.[Methods]Six healthy piglets,weighing(30.0±5.0)kg,were selected and administered by gavage with a Fructus Arctii water extract at 1.0 g/kg·bw.Blood was collected from the anterior vena cava at different time points.The concentration of arctigenin in pig plasma was determined by HPLC.The main pharmacokinetic parameters were:absorption half-life(t1/2 ka)(0.098±0.006)h,distribution half-life(t1/2α)(0.208±0.009)h,elimination half-life(t1/2β)(23.816±2.151)h,apparent volume of distribution(Vd)(32.212±4.033)L/kg,apparent volume of distribution(Vd)(32.212±4.033)L/kg,clearance(CLb)(2.384±1.589)L/(h·kg),peak time(tmax)(0.251±0.011)h,peak concentration(Cmax)(0.560±0.063)μg/ml,and the area under the curve(AUC)at the time of drug administration(9.620±0.752)μg·h/ml.[Results]After oral administration of the water extract powder of Fructus Arctii,arctigenin showed a two-compartment model with absorption in piglets,with the characteristics of fast absorption,wide distribution and slow elimination.Arctigenin might have a hepatoenteral circulation in piglets,and the drug effect could last for a long time.[Conclusions]This study provides a theoretical basis and reference for the development and clinical use of new veterinary drugs.
文摘目的:利用知识图谱可视化展示牛蒡子在中医药领域的研究成果与热点,分析其研究现状及预测研究趋势,为牛蒡子的后续研究提供参考。方法:采用Citespace软件与VOSviewer软件对中国知网(CNKI)和Web of Science(WOS)数据库2001—2021年收录的与牛蒡子研究相关的中文文献537篇、英文文献149篇,分别从发文量、作者、机构、关键词等方面进行计量学分析,绘制网络可视化图谱。结果:中国是对牛蒡子研究最多的国家,中、英文文献中发文量最多的机构均为辽宁中医药大学,载文量最多的中、英文期刊分别为《时珍国医国药》《Phytotherapy Research》。中、英文文献关键词分析结果显示研究方向主要集中于牛蒡子化学成分、药理作用、栽培种植、加工炮制。结论:目前研究热点主要是木脂素类化合物(牛蒡苷及牛蒡苷元)对炎症、肿瘤、糖尿病及其并发症等疾病的预防和治疗,对其抗炎、抗肿瘤、治疗糖尿病等的信号通路、基因靶点及机制的深入研究将是未来牛蒡子研究的主要发展趋势。
基金financially supported by the Natural Science Foundation of Jiangsu Province(BK2011135)Found Project for Transformation of Scientific and Technological Achievements of Jiangsu Province(No.BZ2011053)+1 种基金Open Project of National First Class Key Discipline for Science of Chinese Materia Medica,Nanjing University of Chinese Medicine(No.2011ZYX2-013)Scientific Innovation Research of University Graduate of Jiangsu Province(CXZZ130626)
文摘The present study was designed to optimize the processing of Fructus Arctii by response surface methodology(RSM).Based on single factor studies,a three-variable,three-level Box-Behnken design(BBD) was used to monitor the effects of independent variables,including processing temperature and time,on the dependent variables.Response surfaces and contour plots of the contents of total lignans,chlorogenic acid,arctiin,and arctigenin were obtained through ultraviolet and visible(UV-Vis) monitoring and high performance liquid chromatography(HPLC).Fructus Arctii should be processed under heating in a pot at 311℃,medicine at 119 °C for 123 s with flipping frequently.The experimental values under the optimized processing technology were consistent with the predicted values.In conclusion,RSM is an effective method to optimize the processing of traditional Chinese medicine(TCM).
基金Supported by Major Science and Technology Project"Major New Drug Innovation".
文摘[Objectives] To establish a determination method for the content of total lignanoids in Tangjiangshenkang granules. [Methods] Two-wavelength ultraviolet spectrophotometry (TWBS) was used to scan arctiin control solution, chlorogenic acid control solution and Tangjiangshenkang granule test solution in the range of 200-400 nm. In the ultraviolet scanning diagram of arctiin reference solution, the maximum absorption wavelength of 280 nm was determined as the determination wavelength λ 1, the detection wavelength in the ultraviolet scanning diagram of chlorogenic acid reference solution ( λ 1=280 nm) was determined, and 350 nm was the reference wavelength λ 2;the content of total lignosides in Tangjiangshenkang granules was determined with arctiin as the reference substance. [Results] The precision, accuracy, and durability of this method were fine. The concentration of arctiin was linearly correlated with the absorbance difference in the range of 0.007 95-0.071 55 mg/mL ( r =0. 999 9). The average recovery of arctiin was 100.8%, and the RSD value was 1.04% ( n =6). Calculated as arctiin, three batches of Tangjiangshenkang granules contain no less than 20% of total lignosides. [Conclusions] The method has the advantages of simple operation, good accuracy, precision and reliable stability. It can be used as the content determination and quality control method of total lignosides in Tangjiangshenkang granules.
