Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an import...Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an important ornamental and medicinal value.To reveal the impact of elevational gradient on the habitat soil and plant physiological attributes of this species,we collected root topsoil(0–20 cm)and subsoil(20–40 cm)samples and upper leaves at the initial blooming phase,in a survey of six elevations at 1,257 m,1,538 m,1,744 m,1,970 m,2,135 m,and 2,376 m,with 18 block plots,and 5sampling points at each site.Temperature decreases with an increase in elevation,and soil variables,and enzymatic activities fluctuated in both the topsoil and subsoil,with all of them increasing with elevation and decreasing with soil depth.Redundancy analysis was conducted to explore the correlation between the distribution of A.crenata var.bicolor along the elevational gradient and soil nutrients and enzyme activities,the soil properties were mainly affected by p H at low elevations,and governed by total phosphorus(TP)and available nitrogen(AN)at high elevations.The levels of chlorophyll,carbohydrates,and enzymatic activity except for anthocyanin in this species showed significant variation depending on physiological attributes evaluated at the different collection elevations.The decline in chlorophyll a and b may be associated with the adaptive response to avoid environmental stress,while its higher soluble sugar and protein contents play important roles in escaping adverse climatic conditions,and the increases in activities of antioxidant enzymes except peroxidase(POD)reflect this species’higher capacity for reactive oxygen scavenging(ROS)at high elevations.This study provides supporting evidence that elevation significantly affects the physiological attributes of A.crenata var.bicolor on Gaoligong Mountain,which is helpful for understanding plant adaptation strategies and the plasticity of plant physiological traits along the elevational gradients.展开更多
目的建立HPLC-ELSD法同步测定朱砂根活性成分岩白菜素和百两金皂苷A的含量。方法采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相梯度洗脱,流速1.0 mL min-1,柱温30℃。ELSD参数:漂移管温度100℃,载气流速2....目的建立HPLC-ELSD法同步测定朱砂根活性成分岩白菜素和百两金皂苷A的含量。方法采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相梯度洗脱,流速1.0 mL min-1,柱温30℃。ELSD参数:漂移管温度100℃,载气流速2.5 mL min-1。结果岩白菜素和百两金皂苷A分别在0.42~8.40μg和0.51~10.20μg与峰面积呈良好的线性关系,平均回收率分别为101.2%、101.4%,RSD分别为1.6%、2.0%。结论建立的含量测定方法操作简便、结果准确、重现性好,可用于朱砂根药材的质量控制。展开更多
基金supported by the Doctoral Research Fund Project of Southwest Forestry University(CN)(Grant No.111806)。
文摘Plants overcome environmental stress by generating metabolic pathways.Thus,it is crucial to understand the physiological mechanisms of plant responses to changing environments.Ardisia crenata var.bicolor has an important ornamental and medicinal value.To reveal the impact of elevational gradient on the habitat soil and plant physiological attributes of this species,we collected root topsoil(0–20 cm)and subsoil(20–40 cm)samples and upper leaves at the initial blooming phase,in a survey of six elevations at 1,257 m,1,538 m,1,744 m,1,970 m,2,135 m,and 2,376 m,with 18 block plots,and 5sampling points at each site.Temperature decreases with an increase in elevation,and soil variables,and enzymatic activities fluctuated in both the topsoil and subsoil,with all of them increasing with elevation and decreasing with soil depth.Redundancy analysis was conducted to explore the correlation between the distribution of A.crenata var.bicolor along the elevational gradient and soil nutrients and enzyme activities,the soil properties were mainly affected by p H at low elevations,and governed by total phosphorus(TP)and available nitrogen(AN)at high elevations.The levels of chlorophyll,carbohydrates,and enzymatic activity except for anthocyanin in this species showed significant variation depending on physiological attributes evaluated at the different collection elevations.The decline in chlorophyll a and b may be associated with the adaptive response to avoid environmental stress,while its higher soluble sugar and protein contents play important roles in escaping adverse climatic conditions,and the increases in activities of antioxidant enzymes except peroxidase(POD)reflect this species’higher capacity for reactive oxygen scavenging(ROS)at high elevations.This study provides supporting evidence that elevation significantly affects the physiological attributes of A.crenata var.bicolor on Gaoligong Mountain,which is helpful for understanding plant adaptation strategies and the plasticity of plant physiological traits along the elevational gradients.
文摘目的建立HPLC-ELSD法同步测定朱砂根活性成分岩白菜素和百两金皂苷A的含量。方法采用Phenomenex Luna C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水为流动相梯度洗脱,流速1.0 mL min-1,柱温30℃。ELSD参数:漂移管温度100℃,载气流速2.5 mL min-1。结果岩白菜素和百两金皂苷A分别在0.42~8.40μg和0.51~10.20μg与峰面积呈良好的线性关系,平均回收率分别为101.2%、101.4%,RSD分别为1.6%、2.0%。结论建立的含量测定方法操作简便、结果准确、重现性好,可用于朱砂根药材的质量控制。