[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,an...[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.展开更多
Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from th...Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.展开更多
文摘[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.
基金supported by the Technological Production Transformation Foundation by the Ministry of Science and Technology of China(2002370010495)the foundation of Shandong Fruit Tree Three-Zero Project
文摘Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.
