A High-throughput Genomic Tool： Diversity Array Technology Complementary for Rice Genotyping

Diversity array technology （DART^TM） was a genotyping tool characterized gel-independent and high throughput. The main purpose of present study is to validate DArT for rice （Oryza sativa L.）genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose gene pool, and optimize this genomic tool in order to evaluate rice germplasm genetic diversity. To achieve this, firstly, a generalpurpose DArT array was developed. Ten representatives from 24 varieties were hybridized with the general-purpose array to determine the informativeness of the clones printed on the array. The informative 1 152 clones were re-arrayed on a slide and used to fingerprint 17 of 24 germplasms. Hybridizing targets prepared from the germplasm to be assayed to the DNA array gave DNA fingerprints of germplasms. Raw data were normalized and transformed into binary data, which were then analyzed by using NTSYSpc （Numerical taxonomy system for cluster and ordination analysis, v. 2.02j） software package. The graphically displayed dendrogram derived from the array experimental data was matched with simple sequence repeats genotyping outline and varieties＇ pedigree deviation of the different varieties. Considering DArT is a sequence-independent genotyping approach, it will be applied in studies of the genetic diversity and the gene mapping of diverse of organisms, especially for those crops with less-developed molecular markers.

Beam-pointing error compensation method of phased array radar seeker with phantom-bit technology

A phased array radar seeker（PARS） must be able to effectively decouple body motion and accurately extract the line-of-sight（LOS） rate for target missile tracking.In this study,the realtime two-channel beam pointing error（BPE） compensation method of PARS for LOS rate extraction is designed.The PARS discrete beam motion principium is analyzed,and the mathematical model of beam scanning control is finished.According to the principle of the antenna element shift phase,both the antenna element shift phase law and the causes of beam-pointing error under phantom-bit conditions are analyzed,and the effect of BPE caused by phantom-bit technology（PBT） on the extraction accuracy of the LOS rate is examined.A compensation method is given,which includes coordinate transforms,beam angle margin compensation,and detector dislocation angle calculation.When the method is used,the beam angle margin in the pitch and yaw directions is calculated to reduce the effect of the missile body disturbance and to improve LOS rate extraction precision by compensating for the detector dislocation angle.The simulation results validate the proposed method.

Towed line array sonar platform noise suppression based on spatial matrix filtering technology

The spatial matrix filter was designed and used for solving the problem to detect a weak target who was influenced by the strong nearby platform noise interference of the towed line array sonar. The MFP technology and the DOA estimation technology were combined together by using the sound propagation characteristics of both target and interference. The spatial matrix filter with platform noise zero response constraint was designed by the near-field platform noise normal modes copy vectors and the far-field plane wave bearing vectors together. The optimal solution of the optimization problem for designing the spatial matrix filter was deduced directly, and it was simplified by the generalized singular value decomposition. The total response error to the plane wave bearing vectors and the total response to the platform noise copy vectors were given. The phenomena that strong interferences existed in the bearing course and blind areas existed after filtering were analyzed by the correlation between the plat- form noise copy vectors and the plane wave bearing vectors. It could be found from simulations that it has less blind area and higher detection ability by using the spatial matrix filtering technology.

Analysis of Chinese women with primary ovarian insufficiency by high resolution array-comparative genomic hybridization

Background Primary ovarian insufficiency （POI） is defined as a primary ovarian defect characterized by absent menarche （primary amenorrhea） or premature depletion of ovarian follicles before the age of 40 years. The etiology of primary ovarian insufficiency in human female patients is still unclear. The purpose of this study is to investigate the potential genetic causes in primary amenorrhea patients by high resolution array based comparative genomic hybridization （array-CGH） analysis. Methods Following the standard karyotyping analysis, genomic DNA from whole blood of 15 primary amenorrhea patients and 15 normal control women was hybridized with Affymetrix cytogenetic 2.7M arrays following the standard protocol. Copy number variations identified by array-CGH were confirmed by real time polymerase chain reaction.

Combined experimental and simulation study on corrosion behavior of B10 copper-nickel alloy welded joint under local turbulence

The corrosion behavior of B10 copper-nickel alloy welded joints in seawater pipeline system was analyzed under local turbulence induced by weld residual height.The corrosion behavior was evaluated by array electrode technology,mor-phology and elemental characterization,and COMSOL Multiphysics simulation.The results provide a theoretical basis for the corrosion and leakage of B10 alloy in seawater pipeline under the action of turbulence.The results show that residual height-induced turbulence exhibits a significant effect on the corrosion behavior in different areas of welded joints in B10 alloy.Turbulence can damage some surfaces,causing polarity deflection followed by acceleration of corrosion,or it is easier to form a protective film to slow down corrosion.COMSOL Multiphysics results show that the shear rate and turbulent kinetic energy increase linearly with the increase in residual height and velocity.The corrosion behavior of alloy surface is influenced by controlling the mass transfer rate and surface state.