INTRODUCTION Cell apoptosis,which involves the biologic regulation of the numbers and vital activity of cells,is an important metaboloc process in both normal cells and tumor cells.
It was recently reported that arsenic trioxide （As<sub>2</sub>O<sub>3</sub>） can induce complete remission in patients with acute promyelocytic leukemia （APL）. In this present article, the ...It was recently reported that arsenic trioxide （As<sub>2</sub>O<sub>3</sub>） can induce complete remission in patients with acute promyelocytic leukemia （APL）. In this present article, the biological effect of As<sub>2</sub>O<sub>3</sub> on human cervical cancer HeLa cells and HeLa cells overexpressing Bcl-2 is studied. By MTT and colony forming ability assays, morphology alteration, flow cytometric analysis, DNA gel electrephoresis and in situ cell death detection （TUNEL）, it was found that As<sub>2</sub>O<sub>3</sub> inhibited the growth of HeLa cells and induced G2/M arrest and apoptosis of the cells. RT-PCR, Northern blot, Western blot analysis revealed that As<sub>2</sub>O<sub>3</sub> induced HeLa cell apoptosis possibly via decreasing the expression of c-myc and viral genes. HeLa cells overexpressing Bcl-2 partly resist As<sub>2</sub>O<sub>3</sub> induced apoptosis, which might be relative to preventing the cells from As<sub>2</sub>O<sub>3</sub> caused G2/M block, downregulation of c-myc gene expression and inhibition of viral gene expression was also noted, However, it was found that As<sub>2</sub>O<sub>3</sub> at a high展开更多
Objective To study the effect of arsenic trioxide (As 2O 3) on non APL acute myeloid leukemia (AML) cells and the interreactive effect between retinoic acid (RA) and As 2O 3 Methods RA sensitive (S) and RA ...Objective To study the effect of arsenic trioxide (As 2O 3) on non APL acute myeloid leukemia (AML) cells and the interreactive effect between retinoic acid (RA) and As 2O 3 Methods RA sensitive (S) and RA resistant (R) HL 60 non APL AML cells were used as an in vitro model Cell number and trypan blue were used to observe cell growth and survival Apoptosis was determined by morphological changes, using a DNA laddering assay, terminal deoxynucleotidyl transferase (TdT) fragment end labeling assay and a flow cytometry assay Results As 2O 3 induced apoptosis in both HL 60S and HL 60R cells, As 2O 3 induced apoptosis was both time and concentration dependent in a therapeutically achievable As 2O 3 range (0 25-4.0?μmol/L) Both all trans retinoic acid (ATRA) and 9 cis retinoic acid (9cRA) potentiated As 2O 3 induced apoptosis, as measured by quantitative TdT fragment end labeling and flow cytometry assays in both HL 60S and HL 60R cells ( P <0 05, for all RA+As 2O 3 combinations vs As 2O 3 alone in both sublines) Conclusions As 2O 3 may inhibit the growth of non APL AML cells by promoting programmed cell death RA can potentiate As 2O 3 induced apoptosis even in RA resistant HL 60 cells in which the classical ATRA response pathway is repressed owing to a homozygous inactivating mutation in the retinoic acid receptor α As 2O 3 can have clinical activity in non APL cases of AML and the enhanced activity might result from the combined As 2O 3 RA therapy展开更多
A comprehensive theoretical study on the bimolecular reaction of C2H502 with OH radicals was performed at the CCSD（T）/6-311＋＋G（2df2p）//B3LYP/6-311＋G（d,p） level of theory. The calculation results show that C2H...A comprehensive theoretical study on the bimolecular reaction of C2H502 with OH radicals was performed at the CCSD（T）/6-311＋＋G（2df2p）//B3LYP/6-311＋G（d,p） level of theory. The calculation results show that C2H5O2 ＋ OH reaction proceeds on both the singlet and the triplet potential energy surfaces（PESs）. On the singlet PES, the favorable pathway is the addition of OH radical to the terminal oxygen atom of C2H5O2 radical, leading to the formation of trioxide C2H5O3H with a barrierless process. Then, the trioxide directly decomposes to the products C2H50 and HO2 radicals. On the triplet PES, the predominant pathways are a and β hydrogen atom abstractions of C2H5O2 radical by OH radical-forming products 3CH3CHO2＋H2O and 3CH2CH2O2＋H2O, and the corresponding bar- tiers are 12.02（3TS8） and 19.19 kJ/mol（3TS9）, respectively. In addition, the comprehensive properties of trioxide C2H503H were investigated for the ftrst time. The results indicate that the trioxide complex RC1 can exist stably in the atmosphere owing to a significantly large and negative enthalpy of formation（-118.44 kJ/mol） as well as a high first excitation energy（5.94 eV）.展开更多
文摘INTRODUCTION Cell apoptosis,which involves the biologic regulation of the numbers and vital activity of cells,is an important metaboloc process in both normal cells and tumor cells.
文摘It was recently reported that arsenic trioxide （As<sub>2</sub>O<sub>3</sub>） can induce complete remission in patients with acute promyelocytic leukemia （APL）. In this present article, the biological effect of As<sub>2</sub>O<sub>3</sub> on human cervical cancer HeLa cells and HeLa cells overexpressing Bcl-2 is studied. By MTT and colony forming ability assays, morphology alteration, flow cytometric analysis, DNA gel electrephoresis and in situ cell death detection （TUNEL）, it was found that As<sub>2</sub>O<sub>3</sub> inhibited the growth of HeLa cells and induced G2/M arrest and apoptosis of the cells. RT-PCR, Northern blot, Western blot analysis revealed that As<sub>2</sub>O<sub>3</sub> induced HeLa cell apoptosis possibly via decreasing the expression of c-myc and viral genes. HeLa cells overexpressing Bcl-2 partly resist As<sub>2</sub>O<sub>3</sub> induced apoptosis, which might be relative to preventing the cells from As<sub>2</sub>O<sub>3</sub> caused G2/M block, downregulation of c-myc gene expression and inhibition of viral gene expression was also noted, However, it was found that As<sub>2</sub>O<sub>3</sub> at a high
文摘Objective To study the effect of arsenic trioxide (As 2O 3) on non APL acute myeloid leukemia (AML) cells and the interreactive effect between retinoic acid (RA) and As 2O 3 Methods RA sensitive (S) and RA resistant (R) HL 60 non APL AML cells were used as an in vitro model Cell number and trypan blue were used to observe cell growth and survival Apoptosis was determined by morphological changes, using a DNA laddering assay, terminal deoxynucleotidyl transferase (TdT) fragment end labeling assay and a flow cytometry assay Results As 2O 3 induced apoptosis in both HL 60S and HL 60R cells, As 2O 3 induced apoptosis was both time and concentration dependent in a therapeutically achievable As 2O 3 range (0 25-4.0?μmol/L) Both all trans retinoic acid (ATRA) and 9 cis retinoic acid (9cRA) potentiated As 2O 3 induced apoptosis, as measured by quantitative TdT fragment end labeling and flow cytometry assays in both HL 60S and HL 60R cells ( P <0 05, for all RA+As 2O 3 combinations vs As 2O 3 alone in both sublines) Conclusions As 2O 3 may inhibit the growth of non APL AML cells by promoting programmed cell death RA can potentiate As 2O 3 induced apoptosis even in RA resistant HL 60 cells in which the classical ATRA response pathway is repressed owing to a homozygous inactivating mutation in the retinoic acid receptor α As 2O 3 can have clinical activity in non APL cases of AML and the enhanced activity might result from the combined As 2O 3 RA therapy
基金Supported by the National Natural Science Foundation of China（Nos.21473108, 21473107） and the Fundamental Research Funds for the Central Universities of China（No.GK201603035）.
文摘A comprehensive theoretical study on the bimolecular reaction of C2H502 with OH radicals was performed at the CCSD（T）/6-311＋＋G（2df2p）//B3LYP/6-311＋G（d,p） level of theory. The calculation results show that C2H5O2 ＋ OH reaction proceeds on both the singlet and the triplet potential energy surfaces（PESs）. On the singlet PES, the favorable pathway is the addition of OH radical to the terminal oxygen atom of C2H5O2 radical, leading to the formation of trioxide C2H5O3H with a barrierless process. Then, the trioxide directly decomposes to the products C2H50 and HO2 radicals. On the triplet PES, the predominant pathways are a and β hydrogen atom abstractions of C2H5O2 radical by OH radical-forming products 3CH3CHO2＋H2O and 3CH2CH2O2＋H2O, and the corresponding bar- tiers are 12.02（3TS8） and 19.19 kJ/mol（3TS9）, respectively. In addition, the comprehensive properties of trioxide C2H503H were investigated for the ftrst time. The results indicate that the trioxide complex RC1 can exist stably in the atmosphere owing to a significantly large and negative enthalpy of formation（-118.44 kJ/mol） as well as a high first excitation energy（5.94 eV）.
