[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the co...[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.展开更多
[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield o...[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield of Artemisia argyi leaf in Qichun(AALQ).[Methods]The content of the total flavonoids in AALQ was determined by UV-Vis spectrophotometry with apigenin as reference substance.The contents of volatile oil and tannins were determined by the methods in Chinese Pharmacopoeia(2015 edition).The moxa yield was detected by the self-developed method of grinding and sifting with high-speed pulverizer.[Results]Whether applying cake fertilizer,or compound fertilizer after applying barnyard manure,the contents of total flavonoids and tannins in AALQ were reduced in varying degrees,and the content of volatile oil was changed,which had adversely affect the accumulation of effective substances in the leaves of medicinal A.argyi as a whole.The contents of related chemical substances and moxa yield of A.argyi planted in the neighbouring Xishui County from Qichun,Hubei Province were relatively high.[Conclusions]The contents of effective substances and moxa yield of AALQ were significantly reduced as a whole when applied fertilizer in planting,especially after the application of barnyard manure,compound fertilizer was added.The quality of A.argyi leaf would not be adversely affected if it was transplanted from Qichun County,Hubei Province,which is the genuine producing area,to nearby areas with similar ecological environment.展开更多
目的:研究艾草总黄酮(total flavonoids from Artemisia argyi,TFAA)的最佳提取工艺及抗氧化活性。方法:采用超声辅助提取法,以乙醇浓度、提取温度、提取时间、料液比为考察因素,通过正交实验优选TFAA的最佳提取工艺。采用化学模拟体系...目的:研究艾草总黄酮(total flavonoids from Artemisia argyi,TFAA)的最佳提取工艺及抗氧化活性。方法:采用超声辅助提取法,以乙醇浓度、提取温度、提取时间、料液比为考察因素,通过正交实验优选TFAA的最佳提取工艺。采用化学模拟体系测定TFAA的抗氧化活性。结果:TFAA的最佳提取条件为乙醇浓度60%、提取温度90℃、提取时间1.5 h、料液比1∶45。TFAA清除DPPH.和ABTS.+的IC50分别为38.05μg/mL和31.08μg/mL。结论:经工艺优化后TFAA的提取量为177.5mg/g,具有很强的抗氧化活性。展开更多
文摘[Objectives] To analyze the volatile components,the contents of total flavonoids and total polysaccharides of the roots of Artemisia argyi Levl. et Van. var. argyi cv. Qiai,and to provide a scientific basis for the comprehensive development and utilization of the resources.[Methods]The volatile components of the roots were extracted by solid phase micro-extraction( SPME),and its volatile components were analyzed by gas chromatography-mass spectrometry( GC-MS); the contents of total flavonoids and total polysaccharides in the samples were measured by UV-spectrophotometry. [Results] 44 peaks were isolated from the roots and 30 components were identified,accounting for 80. 9% of the total volatile components; the content of total flavonoids in the roots was 9. 42%; the content of total polysaccharides in the roots was 11. 05%.[Conclusions] After a comprehensive investigation,the olefins in the roots generally have antibacterial activity,the contents of the total flavonoids and total polysaccharides contained in the roots were relatively high,the roots of Artemisia argyi do have broad prospects for the development.
文摘[Objectives]To make a preliminary study of the effects of the fertilizer application and transplantation from Qichun County,Hubei Province,China,to neighbouring area on the chemical substance contents and moxa yield of Artemisia argyi leaf in Qichun(AALQ).[Methods]The content of the total flavonoids in AALQ was determined by UV-Vis spectrophotometry with apigenin as reference substance.The contents of volatile oil and tannins were determined by the methods in Chinese Pharmacopoeia(2015 edition).The moxa yield was detected by the self-developed method of grinding and sifting with high-speed pulverizer.[Results]Whether applying cake fertilizer,or compound fertilizer after applying barnyard manure,the contents of total flavonoids and tannins in AALQ were reduced in varying degrees,and the content of volatile oil was changed,which had adversely affect the accumulation of effective substances in the leaves of medicinal A.argyi as a whole.The contents of related chemical substances and moxa yield of A.argyi planted in the neighbouring Xishui County from Qichun,Hubei Province were relatively high.[Conclusions]The contents of effective substances and moxa yield of AALQ were significantly reduced as a whole when applied fertilizer in planting,especially after the application of barnyard manure,compound fertilizer was added.The quality of A.argyi leaf would not be adversely affected if it was transplanted from Qichun County,Hubei Province,which is the genuine producing area,to nearby areas with similar ecological environment.
